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Q16633

- OBF1_HUMAN

UniProt

Q16633 - OBF1_HUMAN

Protein

POU domain class 2-associating factor 1

Gene

POU2AF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

    GO - Molecular functioni

    1. DNA binding Source: Ensembl
    2. protein binding Source: IntAct
    3. transcription coactivator activity Source: ProtInc
    4. transcription cofactor activity Source: ProtInc

    GO - Biological processi

    1. humoral immune response Source: ProtInc
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription from RNA polymerase II promoter Source: ProtInc

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain class 2-associating factor 1
    Alternative name(s):
    B-cell-specific coactivator OBF-1
    BOB-1
    OCA-B
    OCT-binding factor 1
    Gene namesi
    Name:POU2AF1
    Synonyms:OBF1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:9211. POU2AF1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    Orphaneti186. Primary biliary cirrhosis.
    PharmGKBiPA33535.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256POU domain class 2-associating factor 1PRO_0000058018Add
    BLAST

    Post-translational modificationi

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.Curated

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ16633.
    PRIDEiQ16633.

    PTM databases

    PhosphoSiteiQ16633.

    Expressioni

    Tissue specificityi

    B-cell specific.

    Gene expression databases

    ArrayExpressiQ16633.
    BgeeiQ16633.
    CleanExiHS_POU2AF1.
    GenevestigatoriQ16633.

    Organism-specific databases

    HPAiCAB011193.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SIAH1Q8IUQ42EBI-943588,EBI-747107

    Protein-protein interaction databases

    BioGridi111446. 11 interactions.
    IntActiQ16633. 3 interactions.
    MINTiMINT-206323.
    STRINGi9606.ENSP00000228217.

    Structurei

    Secondary structure

    1
    256
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi28 – 347

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CQTX-ray3.20I/J1-44[»]
    DisProtiDP00172.
    ProteinModelPortaliQ16633.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ16633.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the POU2AF1 family.Curated

    Phylogenomic databases

    eggNOGiNOG80789.
    HOGENOMiHOG000059584.
    HOVERGENiHBG007859.
    InParanoidiQ16633.
    OMAiCLDMEGS.
    OrthoDBiEOG7JDQZ9.
    PhylomeDBiQ16633.
    TreeFamiTF332565.

    Family and domain databases

    InterProiIPR015389. PD-C2-AF1.
    [Graphical view]
    PfamiPF09310. PD-C2-AF1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q16633-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV    50
    VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA 100
    PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN 150
    VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL 200
    PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT 250
    LSVEGF 256
    Length:256
    Mass (Da):27,436
    Last modified:November 1, 1996 - v1
    Checksum:i2C46F1796774D614
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti141 – 1411T → A.
    Corresponds to variant rs1042750 [ dbSNP | Ensembl ].
    VAR_005521
    Natural varianti194 – 1941Q → R.
    Corresponds to variant rs1042751 [ dbSNP | Ensembl ].
    VAR_005522

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47550 mRNA. Translation: CAA87630.1.
    X83504 mRNA. Translation: CAA58494.1.
    Z49194 mRNA. Translation: CAA89053.1.
    AK313573 mRNA. Translation: BAG36346.1.
    CH471065 Genomic DNA. Translation: EAW67137.1.
    BC032549 mRNA. Translation: AAH32549.1.
    CCDSiCCDS31675.1.
    PIRiA55652.
    RefSeqiNP_006226.2. NM_006235.2.
    UniGeneiHs.654525.

    Genome annotation databases

    EnsembliENST00000393067; ENSP00000376786; ENSG00000110777.
    GeneIDi5450.
    KEGGihsa:5450.
    UCSCiuc001plg.4. human.

    Polymorphism databases

    DMDMi2833276.

    Keywords - Coding sequence diversityi

    Chromosomal rearrangement, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47550 mRNA. Translation: CAA87630.1 .
    X83504 mRNA. Translation: CAA58494.1 .
    Z49194 mRNA. Translation: CAA89053.1 .
    AK313573 mRNA. Translation: BAG36346.1 .
    CH471065 Genomic DNA. Translation: EAW67137.1 .
    BC032549 mRNA. Translation: AAH32549.1 .
    CCDSi CCDS31675.1.
    PIRi A55652.
    RefSeqi NP_006226.2. NM_006235.2.
    UniGenei Hs.654525.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CQT X-ray 3.20 I/J 1-44 [» ]
    DisProti DP00172.
    ProteinModelPortali Q16633.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111446. 11 interactions.
    IntActi Q16633. 3 interactions.
    MINTi MINT-206323.
    STRINGi 9606.ENSP00000228217.

    PTM databases

    PhosphoSitei Q16633.

    Polymorphism databases

    DMDMi 2833276.

    Proteomic databases

    MaxQBi Q16633.
    PRIDEi Q16633.

    Protocols and materials databases

    DNASUi 5450.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000393067 ; ENSP00000376786 ; ENSG00000110777 .
    GeneIDi 5450.
    KEGGi hsa:5450.
    UCSCi uc001plg.4. human.

    Organism-specific databases

    CTDi 5450.
    GeneCardsi GC11M111222.
    HGNCi HGNC:9211. POU2AF1.
    HPAi CAB011193.
    MIMi 601206. gene.
    neXtProti NX_Q16633.
    Orphaneti 186. Primary biliary cirrhosis.
    PharmGKBi PA33535.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG80789.
    HOGENOMi HOG000059584.
    HOVERGENi HBG007859.
    InParanoidi Q16633.
    OMAi CLDMEGS.
    OrthoDBi EOG7JDQZ9.
    PhylomeDBi Q16633.
    TreeFami TF332565.

    Miscellaneous databases

    EvolutionaryTracei Q16633.
    GeneWikii POU2AF1.
    GenomeRNAii 5450.
    NextBioi 21093.
    PROi Q16633.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q16633.
    Bgeei Q16633.
    CleanExi HS_POU2AF1.
    Genevestigatori Q16633.

    Family and domain databases

    InterProi IPR015389. PD-C2-AF1.
    [Graphical view ]
    Pfami PF09310. PD-C2-AF1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins."
      Strubin M., Newell J.W., Matthias P.
      Cell 80:497-506(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    2. "A B-cell coactivator of octamer-binding transcription factors."
      Gstaiger M., Knoepfel L., Georgiev O., Schaffner W., Hovens C.M.
      Nature 373:360-362(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Peripheral blood lymphocyte.
    3. "Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation."
      Galiegue-Zouitina S., Quief S., Hildebrand M.-P., Denis C., Lecocq G., Collyn-D'Hooghe M., Bastard C., Yuille M., Dyer M.J., Kerckaert J.-P.
      C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphoma.
    4. "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B."
      Luo Y., Roeder R.G.
      Mol. Cell. Biol. 15:4115-4124(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-27 AND 235-256.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Small intestine.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lymph.
    8. "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1."
      Tiedt R., Bartholdy B.A., Matthias G., Newell J.W., Matthias P.
      EMBO J. 20:4143-4152(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SIAH1, DEGRADATION.
    9. "Regulation of BOB.1/OBF.1 stability by SIAH."
      Boehm J., He Y., Greiner A., Staudt L., Wirth T.
      EMBO J. 20:4153-4162(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SIAH1 AND SIAH2, DEGRADATION.
    10. "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface."
      Chasman D., Cepek K., Sharp P.A., Pabo C.O.
      Genes Dev. 13:2650-2657(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 1-44 IN COMPLEX WITH OCT1 AND DNA.

    Entry informationi

    Entry nameiOBF1_HUMAN
    AccessioniPrimary (citable) accession number: Q16633
    Secondary accession number(s): B2R8Z9, Q14983
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3