Reviewed,
UniProtKB/Swiss-Prot Q16633 (OBF1_HUMAN)
Last modified
November 25, 2008.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (7) |
Third-party data |
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Names and origin
| Protein names | Recommended name: POU domain class 2-associating factor 1 Alternative name(s): B-cell-specific coactivator OBF-1 OCT-binding factor 1 BOB-1 OCA-B | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 256 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
| Subcellular location | |
| Tissue specificity | B-cell specific. |
| Post-translational modification | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation Probable. |
| Involvement in disease | A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. |
| Sequence similarities | Belongs to the POU2AF1 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Coding sequence diversity | Chromosomal rearrangement Polymorphism |
| Disease | Proto-oncogene |
| PTM | Ubl conjugation |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | humoral immune response Ref.1 Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Syk | P48025 | 1 | EBI-943588,EBI-300116 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 256 | 256 | POU domain class 2-associating factor 1 | PRO_0000058018 | |||||||
Natural variations | |||||||||||
| Natural variant | 141 | 1 | T → A | VAR_005521 | |||||||
| Natural variant | 194 | 1 | Q → R | VAR_005522 | |||||||
Secondary structure | |||||||||||
Helix Strand Turn | |||||||||||
| Helix | 28 – 34 | 7 | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." Strubin M., Newell J.W., Matthias P. Cell 80:497-506(1995) [PubMed: 7859290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen. |
| [2] | "A B-cell coactivator of octamer-binding transcription factors." Gstaiger M., Knoepfel L., Georgiev O., Schaffner W., Hovens C.M. Nature 373:360-362(1995) [PubMed: 7779176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood lymphocyte. |
| [3] | "Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation." Galiegue-Zouitina S., Quief S., Hildebrand M.-P., Denis C., Lecocq G., Collyn-D'Hooghe M., Bastard C., Yuille M., Dyer M.J., Kerckaert J.-P. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995) [PubMed: 8574789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma. |
| [4] | "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B." Luo Y., Roeder R.G. Mol. Cell. Biol. 15:4115-4124(1995) [PubMed: 7623806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-27 AND 235-256. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph. |
| [6] | "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." Tiedt R., Bartholdy B.A., Matthias G., Newell J.W., Matthias P. EMBO J. 20:4143-4152(2001) [PubMed: 11483517] [Abstract] Cited for: INTERACTION WITH SIAH1, DEGRADATION. |
| [7] | "Regulation of BOB.1/OBF.1 stability by SIAH." Boehm J., He Y., Greiner A., Staudt L., Wirth T. EMBO J. 20:4153-4162(2001) [PubMed: 11483518] [Abstract] Cited for: INTERACTION WITH SIAH1 AND SIAH2, DEGRADATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Z47550 mRNA. Translation: CAA87630.1. X83504 mRNA. Translation: CAA58494.1. Z49194 mRNA. Translation: CAA89053.1. BC032549 mRNA. Translation: AAH32549.1. | |||||||||||||
| PIR | A55652. | ||||||||||||
| RefSeq | NP_006226.2. | ||||||||||||
| UniGene | Hs.654525 | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| DisProt | DP00172. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | Q16633. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000110777. Homo sapiens. [Contig view] | ||||||||||||
| GeneID | 5450. | ||||||||||||
| KEGG | hsa:5450. | ||||||||||||
Organism-specific databases | |||||||||||||
| H-InvDB | HIX0010102. | ||||||||||||
| HGNC | HGNC:9211. POU2AF1. | ||||||||||||
| HPA | CAB011193. | ||||||||||||
| MIM | 601206. gene. | ||||||||||||
| PharmGKB | PA33535. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q16633. | ||||||||||||
| HOVERGEN | Q16633. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q16633. | ||||||||||||
| CleanEx | HS_POU2AF1. | ||||||||||||
| GermOnline | ENSG00000110777. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR015389. PD-C2-AF1. [Graphical view] | ||||||||||||
| Pfam | PF09310. PD-C2-AF1. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 21093. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | OBF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q16633 Secondary accession number(s): Q14983 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


