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Protein

POU domain class 2-associating factor 1

Gene

POU2AF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

GO - Molecular functioni

  • DNA binding Source: Ensembl
  • transcription coactivator activity Source: ProtInc
  • transcription cofactor activity Source: ProtInc

GO - Biological processi

  • humoral immune response Source: ProtInc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110777-MONOMER.
SIGNORiQ16633.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain class 2-associating factor 1
Alternative name(s):
B-cell-specific coactivator OBF-1
BOB-1
OCA-B
OCT-binding factor 1
Gene namesi
Name:POU2AF1
Synonyms:OBF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9211. POU2AF1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi5450.
MalaCardsiPOU2AF1.
OpenTargetsiENSG00000110777.
Orphaneti186. Primary biliary cirrhosis.
PharmGKBiPA33535.

Polymorphism and mutation databases

BioMutaiPOU2AF1.
DMDMi2833276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580181 – 256POU domain class 2-associating factor 1Add BLAST256

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ16633.
PaxDbiQ16633.
PeptideAtlasiQ16633.
PRIDEiQ16633.

PTM databases

iPTMnetiQ16633.
PhosphoSitePlusiQ16633.

Expressioni

Tissue specificityi

B-cell specific.

Gene expression databases

BgeeiENSG00000110777.
CleanExiHS_POU2AF1.
ExpressionAtlasiQ16633. baseline and differential.
GenevisibleiQ16633. HS.

Organism-specific databases

HPAiCAB011193.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SIAH1Q8IUQ42EBI-943588,EBI-747107

Protein-protein interaction databases

BioGridi111446. 12 interactors.
IntActiQ16633. 9 interactors.
MINTiMINT-206323.
STRINGi9606.ENSP00000376786.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi28 – 34Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CQTX-ray3.20I/J1-44[»]
DisProtiDP00172.
ProteinModelPortaliQ16633.
SMRiQ16633.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16633.

Family & Domainsi

Sequence similaritiesi

Belongs to the POU2AF1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJXZ. Eukaryota.
ENOG41123CN. LUCA.
GeneTreeiENSGT00390000017499.
HOGENOMiHOG000059584.
HOVERGENiHBG007859.
InParanoidiQ16633.
OMAiKPYQGVR.
OrthoDBiEOG091G0EUL.
PhylomeDBiQ16633.
TreeFamiTF332565.

Family and domain databases

InterProiIPR015389. PD-C2-AF1.
[Graphical view]
PfamiPF09310. PD-C2-AF1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV
60 70 80 90 100
VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA
110 120 130 140 150
PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN
160 170 180 190 200
VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL
210 220 230 240 250
PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT

LSVEGF
Length:256
Mass (Da):27,436
Last modified:November 1, 1996 - v1
Checksum:i2C46F1796774D614
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_005521141T → A.Corresponds to variant rs1042750dbSNPEnsembl.1
Natural variantiVAR_005522194Q → R.Corresponds to variant rs1042751dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47550 mRNA. Translation: CAA87630.1.
X83504 mRNA. Translation: CAA58494.1.
Z49194 mRNA. Translation: CAA89053.1.
AK313573 mRNA. Translation: BAG36346.1.
CH471065 Genomic DNA. Translation: EAW67137.1.
BC032549 mRNA. Translation: AAH32549.1.
CCDSiCCDS31675.1.
PIRiA55652.
RefSeqiNP_006226.2. NM_006235.2.
UniGeneiHs.654525.

Genome annotation databases

EnsembliENST00000393067; ENSP00000376786; ENSG00000110777.
GeneIDi5450.
KEGGihsa:5450.
UCSCiuc001plg.5. human.

Keywords - Coding sequence diversityi

Chromosomal rearrangement, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47550 mRNA. Translation: CAA87630.1.
X83504 mRNA. Translation: CAA58494.1.
Z49194 mRNA. Translation: CAA89053.1.
AK313573 mRNA. Translation: BAG36346.1.
CH471065 Genomic DNA. Translation: EAW67137.1.
BC032549 mRNA. Translation: AAH32549.1.
CCDSiCCDS31675.1.
PIRiA55652.
RefSeqiNP_006226.2. NM_006235.2.
UniGeneiHs.654525.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CQTX-ray3.20I/J1-44[»]
DisProtiDP00172.
ProteinModelPortaliQ16633.
SMRiQ16633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111446. 12 interactors.
IntActiQ16633. 9 interactors.
MINTiMINT-206323.
STRINGi9606.ENSP00000376786.

PTM databases

iPTMnetiQ16633.
PhosphoSitePlusiQ16633.

Polymorphism and mutation databases

BioMutaiPOU2AF1.
DMDMi2833276.

Proteomic databases

MaxQBiQ16633.
PaxDbiQ16633.
PeptideAtlasiQ16633.
PRIDEiQ16633.

Protocols and materials databases

DNASUi5450.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393067; ENSP00000376786; ENSG00000110777.
GeneIDi5450.
KEGGihsa:5450.
UCSCiuc001plg.5. human.

Organism-specific databases

CTDi5450.
DisGeNETi5450.
GeneCardsiPOU2AF1.
HGNCiHGNC:9211. POU2AF1.
HPAiCAB011193.
MalaCardsiPOU2AF1.
MIMi601206. gene.
neXtProtiNX_Q16633.
OpenTargetsiENSG00000110777.
Orphaneti186. Primary biliary cirrhosis.
PharmGKBiPA33535.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJXZ. Eukaryota.
ENOG41123CN. LUCA.
GeneTreeiENSGT00390000017499.
HOGENOMiHOG000059584.
HOVERGENiHBG007859.
InParanoidiQ16633.
OMAiKPYQGVR.
OrthoDBiEOG091G0EUL.
PhylomeDBiQ16633.
TreeFamiTF332565.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000110777-MONOMER.
SIGNORiQ16633.

Miscellaneous databases

ChiTaRSiPOU2AF1. human.
EvolutionaryTraceiQ16633.
GeneWikiiPOU2AF1.
GenomeRNAii5450.
PROiQ16633.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110777.
CleanExiHS_POU2AF1.
ExpressionAtlasiQ16633. baseline and differential.
GenevisibleiQ16633. HS.

Family and domain databases

InterProiIPR015389. PD-C2-AF1.
[Graphical view]
PfamiPF09310. PD-C2-AF1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOBF1_HUMAN
AccessioniPrimary (citable) accession number: Q16633
Secondary accession number(s): B2R8Z9, Q14983
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.