Reviewed, UniProtKB/Swiss-Prot Q16633 (OBF1_HUMAN)

Names and origin

Protein names

Recommended name:
    POU domain class 2-associating factor 1
Alternative name(s):
    B-cell-specific coactivator OBF-1
    OCT-binding factor 1
    BOB-1
    OCA-B

Gene names

Name:	POU2AF1
Synonyms:	OBF1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Subcellular location	Nucleus.
Tissue specificity	B-cell specific.
Post-translational modification	Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation Probable.
Involvement in disease	A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
Sequence similarities	Belongs to the POU2AF1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Chromosomal rearrangement Polymorphism
Disease	Proto-oncogene
PTM	Ubl conjugation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	humoral immune response Ref.1 Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Syk	P48025	1	EBI-943588,EBI-300116	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 256

256

POU domain class 2-associating factor 1

PRO_0000058018

Natural variations

Natural variant

141

1

T → A

VAR_005521

Natural variant

194

1

Q → R

VAR_005522

Secondary structure

1

.

.

.

256

Helix Strand Turn

Details...

Helix

28 – 34

7

Sequences

	Sequence		Length	Mass (Da)
	Q16633-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2C46F1796774D614	FASTA	256	27,436
	10 20 30 40 50 60 MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY 70 80 90 100 110 120 TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA 130 140 150 160 170 180 DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP 190 200 210 220 230 240 QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE 250 DSDAYALNHT LSVEGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." Strubin M., Newell J.W., Matthias P. Cell 80:497-506(1995) [PubMed: 7859290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[2]	"A B-cell coactivator of octamer-binding transcription factors." Gstaiger M., Knoepfel L., Georgiev O., Schaffner W., Hovens C.M. Nature 373:360-362(1995) [PubMed: 7779176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood lymphocyte.
[3]	"Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation." Galiegue-Zouitina S., Quief S., Hildebrand M.-P., Denis C., Lecocq G., Collyn-D'Hooghe M., Bastard C., Yuille M., Dyer M.J., Kerckaert J.-P. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995) [PubMed: 8574789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma.
[4]	"Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B." Luo Y., Roeder R.G. Mol. Cell. Biol. 15:4115-4124(1995) [PubMed: 7623806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-27 AND 235-256.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[6]	"The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." Tiedt R., Bartholdy B.A., Matthias G., Newell J.W., Matthias P. EMBO J. 20:4143-4152(2001) [PubMed: 11483517] [Abstract] Cited for: INTERACTION WITH SIAH1, DEGRADATION.
[7]	"Regulation of BOB.1/OBF.1 stability by SIAH." Boehm J., He Y., Greiner A., Staudt L., Wirth T. EMBO J. 20:4153-4162(2001) [PubMed: 11483518] [Abstract] Cited for: INTERACTION WITH SIAH1 AND SIAH2, DEGRADATION.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z47550 mRNA. Translation: CAA87630.1.
X83504 mRNA. Translation: CAA58494.1.
Z49194 mRNA. Translation: CAA89053.1.
BC032549 mRNA. Translation: AAH32549.1.

PIR

A55652.

RefSeq

NP_006226.2.

UniGene

Hs.654525

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CQT	X-ray	3.20	I/J	1-44	[»]

DisProt

DP00172.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q16633.

Genome annotation databases

Ensembl

ENSG00000110777. Homo sapiens. [Contig view]

GeneID

5450.

KEGG

hsa:5450.

Organism-specific databases

H-InvDB

HIX0010102.

HGNC

HGNC:9211. POU2AF1.

HPA

CAB011193.

MIM

601206. gene.

PharmGKB

PA33535.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q16633.

HOVERGEN

Q16633.

Gene expression databases

ArrayExpress

Q16633.

CleanEx

HS_POU2AF1.

GermOnline

ENSG00000110777. Homo sapiens.

Family and domain databases

InterPro

IPR015389. PD-C2-AF1.
[Graphical view]

Pfam

PF09310. PD-C2-AF1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

21093.

SOURCE

Search...

Entry information

Entry name

OBF1_HUMAN

Accession

Primary (citable) accession number: Q16633
Secondary accession number(s): Q14983

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)