Q16633 (OBF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 105.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: POU domain class 2-associating factor 1 Alternative name(s): B-cell-specific coactivator OBF-1 BOB-1 OCA-B OCT-binding factor 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 256 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
| Subcellular location | |
| Tissue specificity | B-cell specific. |
| Post-translational modification | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation Probable. Ref.8 Ref.9 |
| Involvement in disease | Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. |
| Sequence similarities | Belongs to the POU2AF1 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Coding sequence diversity | Chromosomal rearrangement Polymorphism |
| Disease | Proto-oncogene |
| PTM | Ubl conjugation |
| Technical term | 3D-structure Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | humoral immune response Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoterTraceable author statement. Source: ProtInc |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | transcription coactivator activity Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 256 | 256 | POU domain class 2-associating factor 1 | PRO_0000058018 | |||||||
Natural variations | |||||||||||
| Natural variant | 141 | 1 | T → A. Corresponds to variant rs1042750 [ dbSNP | Ensembl ]. | VAR_005521 | |||||||
| Natural variant | 194 | 1 | Q → R. Corresponds to variant rs1042751 [ dbSNP | Ensembl ]. | VAR_005522 | |||||||
Secondary structure | |||||||||||
Helix Strand Turn | |||||||||||
| Helix | 28 – 34 | 7 | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." Strubin M., Newell J.W., Matthias P. Cell 80:497-506(1995) [PubMed: 7859290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen. |
| [2] | "A B-cell coactivator of octamer-binding transcription factors." Gstaiger M., Knoepfel L., Georgiev O., Schaffner W., Hovens C.M. Nature 373:360-362(1995) [PubMed: 7779176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood lymphocyte. |
| [3] | "Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation." Galiegue-Zouitina S., Quief S., Hildebrand M.-P., Denis C., Lecocq G., Collyn-D'Hooghe M., Bastard C., Yuille M., Dyer M.J., Kerckaert J.-P. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995) [PubMed: 8574789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma. |
| [4] | "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B." Luo Y., Roeder R.G. Mol. Cell. Biol. 15:4115-4124(1995) [PubMed: 7623806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-27 AND 235-256. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Small intestine. |
| [6] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph. |
| [8] | "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1." Tiedt R., Bartholdy B.A., Matthias G., Newell J.W., Matthias P. EMBO J. 20:4143-4152(2001) [PubMed: 11483517] [Abstract] Cited for: INTERACTION WITH SIAH1, DEGRADATION. |
| [9] | "Regulation of BOB.1/OBF.1 stability by SIAH." Boehm J., He Y., Greiner A., Staudt L., Wirth T. EMBO J. 20:4153-4162(2001) [PubMed: 11483518] [Abstract] Cited for: INTERACTION WITH SIAH1 AND SIAH2, DEGRADATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | Z47550 mRNA. Translation: CAA87630.1. X83504 mRNA. Translation: CAA58494.1. Z49194 mRNA. Translation: CAA89053.1. AK313573 mRNA. Translation: BAG36346.1. CH471065 Genomic DNA. Translation: EAW67137.1. BC032549 mRNA. Translation: AAH32549.1. | ||||||||||||
| IPI | IPI00293727. | ||||||||||||
| PIR | A55652. | ||||||||||||
| RefSeq | NP_006226.2. NM_006235.2. | ||||||||||||
| UniGene | Hs.654525. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q16633. | ||||||||||||
| DisProt | DP00172. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | Q16633. 2 interactions. | ||||||||||||
| STRING | Q16633. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q16633. | ||||||||||||
Polymorphism databases | |||||||||||||
| DMDM | 2833276. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | Q16633. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000228217; ENSP00000228217; ENSG00000110777. | ||||||||||||
| GeneID | 5450. | ||||||||||||
| KEGG | hsa:5450. | ||||||||||||
| UCSC | uc001plg.2. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 5450. | ||||||||||||
| GeneCards | GC11M111222. | ||||||||||||
| H-InvDB | HIX0010102. | ||||||||||||
| HGNC | HGNC:9211. POU2AF1. | ||||||||||||
| HPA | CAB011193. | ||||||||||||
| MIM | 601206. gene. | ||||||||||||
| neXtProt | NX_Q16633. | ||||||||||||
| PharmGKB | PA33535. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | prNOG09488. | ||||||||||||
| GeneTree | ENSGT00390000017499. | ||||||||||||
| HOGENOM | HBG444267. | ||||||||||||
| HOVERGEN | HBG007859. | ||||||||||||
| InParanoid | Q16633. | ||||||||||||
| OMA | YSTDMYV. | ||||||||||||
| OrthoDB | EOG495ZTD. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q16633. | ||||||||||||
| Bgee | Q16633. | ||||||||||||
| CleanEx | HS_POU2AF1. | ||||||||||||
| Genevestigator | Q16633. | ||||||||||||
| GermOnline | ENSG00000110777. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR015389. PD-C2-AF1. [Graphical view] | ||||||||||||
| Pfam | PF09310. PD-C2-AF1. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| NextBio | 21093. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | OBF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q16633 Secondary accession number(s): B2R8Z9, Q14983 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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