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Protein

Serine/arginine-rich splicing factor 7

Gene

SRSF7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri104 – 120CCHC-typePROSITE-ProRule annotationAdd BLAST17

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: Reactome
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • RNA export from nucleus Source: Reactome
  • RNA splicing Source: ProtInc
  • termination of RNA polymerase II transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115875-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiQ16629.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 7
Alternative name(s):
Splicing factor 9G8
Splicing factor, arginine/serine-rich 7
Gene namesi
Name:SRSF7
Synonyms:SFRS7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:10789. SRSF7.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87 – 88RR → EE: Abolishes interaction with NXF1. 1 Publication2
Mutagenesisi97 – 98RR → EE: Abolishes interaction with NXF1. 1 Publication2

Organism-specific databases

DisGeNETi6432.
OpenTargetsiENSG00000115875.
PharmGKBiPA35705.

Polymorphism and mutation databases

BioMutaiSRSF7.
DMDMi3929380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819321 – 238Serine/arginine-rich splicing factor 7Add BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24N6-acetyllysineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei163PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei181PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei192PhosphoserineCombined sources1
Modified residuei194PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei233PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ16629.
MaxQBiQ16629.
PaxDbiQ16629.
PeptideAtlasiQ16629.
PRIDEiQ16629.
TopDownProteomicsiQ16629-1. [Q16629-1]
Q16629-2. [Q16629-2]
Q16629-3. [Q16629-3]
Q16629-4. [Q16629-4]

2D gel databases

SWISS-2DPAGEP99058.

PTM databases

iPTMnetiQ16629.
PhosphoSitePlusiQ16629.
SwissPalmiQ16629.

Expressioni

Tissue specificityi

Brain, liver, kidney and lung.

Gene expression databases

BgeeiENSG00000115875.
CleanExiHS_SFRS7.
ExpressionAtlasiQ16629. baseline and differential.
GenevisibleiQ16629. HS.

Organism-specific databases

HPAiHPA043850.
HPA056926.

Interactioni

Subunit structurei

Found in large molecular weight complexes containing CCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interacts with CCNL2 and CPSF6. Interacts with NXF1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LUC7L2Q9Y3833EBI-398885,EBI-352851
NXF1Q9UBU94EBI-398885,EBI-398874
RBBP6Q7Z6E93EBI-398885,EBI-2117026
SDCBPO005605EBI-398885,EBI-727004
Srek1Q9JKL73EBI-398885,EBI-6452221From a different organism.
SRPK1Q96SB42EBI-398885,EBI-539478
SRPK2P783622EBI-398885,EBI-593303

Protein-protein interaction databases

BioGridi112330. 108 interactors.
DIPiDIP-31790N.
IntActiQ16629. 36 interactors.
MINTiMINT-3032898.
STRINGi9606.ENSP00000325905.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 16Combined sources5
Helixi24 – 34Combined sources11
Beta strandi38 – 53Combined sources16
Helixi57 – 69Combined sources13
Beta strandi72 – 74Combined sources3
Beta strandi78 – 84Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HVZNMR-A12-98[»]
ProteinModelPortaliQ16629.
SMRiQ16629.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16629.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 84RRMPROSITE-ProRule annotationAdd BLAST74
Repeati153 – 16018
Repeati161 – 16828
Repeati169 – 17638
Repeati177 – 18448
Repeati211 – 2185; approximate8
Repeati219 – 2266; approximate8

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 98Sufficient for interaction with NXF1Add BLAST18
Regioni153 – 2266 X 8 AA repeats of R-R-S-R-S-X-S-XAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 238Arg/Ser-rich (RS domain)Add BLAST118

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri104 – 120CCHC-typePROSITE-ProRule annotationAdd BLAST17

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000276234.
HOVERGENiHBG107480.
InParanoidiQ16629.
KOiK12896.
OMAiASPERMG.
OrthoDBiEOG091G17YQ.
PhylomeDBiQ16629.
TreeFamiTF351858.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Isoforms, often lacking the RS domain and differentially expressed in fetal tissues, may be involved in modulation of 9G8 function.
Isoform 1 (identifier: Q16629-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRYGRYGGE TKVYVGNLGT GAGKGELERA FSYYGPLRTV WIARNPPGFA
60 70 80 90 100
FVEFEDPRDA EDAVRGLDGK VICGSRVRVE LSTGMPRRSR FDRPPARRPF
110 120 130 140 150
DPNDRCYECG EKGHYAYDCH RYSRRRRSRS RSRSHSRSRG RRYSRSRSRS
160 170 180 190 200
RGRRSRSASP RRSRSISLRR SRSASLRRSR SGSIKGSRYF QSPSRSRSRS
210 220 230
RSISRPRSSR SKSRSPSPKR SRSPSGSPRR SASPERMD
Length:238
Mass (Da):27,367
Last modified:November 1, 1996 - v1
Checksum:i49136754D9630853
GO
Isoform 2 (identifier: Q16629-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-135: SRSRSH → AENLRR
     136-238: Missing.

Show »
Length:135
Mass (Da):15,573
Checksum:iD390DEB80ADEA752
GO
Isoform 3 (identifier: Q16629-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-132: SRS → YLF
     133-238: Missing.

Show »
Length:132
Mass (Da):15,257
Checksum:iF586F82752DEDCF0
GO
Isoform 4 (identifier: Q16629-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     209-220: Missing.

Note: No experimental confirmation available.
Show »
Length:226
Mass (Da):26,012
Checksum:iA2FAEE66A5E4DCE7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56D → G in BAG59658 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005872130 – 135SRSRSH → AENLRR in isoform 2. Curated6
Alternative sequenceiVSP_005874130 – 132SRS → YLF in isoform 3. Curated3
Alternative sequenceiVSP_005875133 – 238Missing in isoform 3. CuratedAdd BLAST106
Alternative sequenceiVSP_005873136 – 238Missing in isoform 2. CuratedAdd BLAST103
Alternative sequenceiVSP_045840209 – 220Missing in isoform 4. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22253 mRNA. Translation: AAA35495.1.
L41887 Genomic DNA. Translation: AAA88098.1.
AB445102 mRNA. Translation: BAI99737.1.
AY166860 mRNA. Translation: AAN87842.1.
BT006745 mRNA. Translation: AAP35391.1.
AY513287 mRNA. Translation: AAT08040.1.
AK297161 mRNA. Translation: BAG59658.1.
AC074366 Genomic DNA. Translation: AAX93102.1.
CH471053 Genomic DNA. Translation: EAX00365.1.
CH471053 Genomic DNA. Translation: EAX00366.1.
BC000997 mRNA. Translation: AAH00997.1.
BC017369 mRNA. Translation: AAH17369.1.
BC017908 mRNA. Translation: AAH17908.1.
BC022328 mRNA. Translation: AAH22328.1.
CCDSiCCDS33183.1. [Q16629-1]
CCDS56115.1. [Q16629-4]
PIRiA57198.
RefSeqiNP_001026854.1. NM_001031684.2. [Q16629-1]
NP_001182375.1. NM_001195446.1. [Q16629-4]
UniGeneiHs.309090.

Genome annotation databases

EnsembliENST00000313117; ENSP00000325905; ENSG00000115875. [Q16629-1]
ENST00000431066; ENSP00000398159; ENSG00000115875. [Q16629-3]
ENST00000443213; ENSP00000402544; ENSG00000115875. [Q16629-3]
ENST00000446327; ENSP00000402264; ENSG00000115875. [Q16629-4]
GeneIDi6432.
KEGGihsa:6432.
UCSCiuc002rqz.3. human. [Q16629-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22253 mRNA. Translation: AAA35495.1.
L41887 Genomic DNA. Translation: AAA88098.1.
AB445102 mRNA. Translation: BAI99737.1.
AY166860 mRNA. Translation: AAN87842.1.
BT006745 mRNA. Translation: AAP35391.1.
AY513287 mRNA. Translation: AAT08040.1.
AK297161 mRNA. Translation: BAG59658.1.
AC074366 Genomic DNA. Translation: AAX93102.1.
CH471053 Genomic DNA. Translation: EAX00365.1.
CH471053 Genomic DNA. Translation: EAX00366.1.
BC000997 mRNA. Translation: AAH00997.1.
BC017369 mRNA. Translation: AAH17369.1.
BC017908 mRNA. Translation: AAH17908.1.
BC022328 mRNA. Translation: AAH22328.1.
CCDSiCCDS33183.1. [Q16629-1]
CCDS56115.1. [Q16629-4]
PIRiA57198.
RefSeqiNP_001026854.1. NM_001031684.2. [Q16629-1]
NP_001182375.1. NM_001195446.1. [Q16629-4]
UniGeneiHs.309090.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HVZNMR-A12-98[»]
ProteinModelPortaliQ16629.
SMRiQ16629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112330. 108 interactors.
DIPiDIP-31790N.
IntActiQ16629. 36 interactors.
MINTiMINT-3032898.
STRINGi9606.ENSP00000325905.

PTM databases

iPTMnetiQ16629.
PhosphoSitePlusiQ16629.
SwissPalmiQ16629.

Polymorphism and mutation databases

BioMutaiSRSF7.
DMDMi3929380.

2D gel databases

SWISS-2DPAGEP99058.

Proteomic databases

EPDiQ16629.
MaxQBiQ16629.
PaxDbiQ16629.
PeptideAtlasiQ16629.
PRIDEiQ16629.
TopDownProteomicsiQ16629-1. [Q16629-1]
Q16629-2. [Q16629-2]
Q16629-3. [Q16629-3]
Q16629-4. [Q16629-4]

Protocols and materials databases

DNASUi6432.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313117; ENSP00000325905; ENSG00000115875. [Q16629-1]
ENST00000431066; ENSP00000398159; ENSG00000115875. [Q16629-3]
ENST00000443213; ENSP00000402544; ENSG00000115875. [Q16629-3]
ENST00000446327; ENSP00000402264; ENSG00000115875. [Q16629-4]
GeneIDi6432.
KEGGihsa:6432.
UCSCiuc002rqz.3. human. [Q16629-1]

Organism-specific databases

CTDi6432.
DisGeNETi6432.
GeneCardsiSRSF7.
HGNCiHGNC:10789. SRSF7.
HPAiHPA043850.
HPA056926.
MIMi600572. gene.
neXtProtiNX_Q16629.
OpenTargetsiENSG00000115875.
PharmGKBiPA35705.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0107. Eukaryota.
ENOG4111N8Y. LUCA.
GeneTreeiENSGT00700000104103.
HOGENOMiHOG000276234.
HOVERGENiHBG107480.
InParanoidiQ16629.
KOiK12896.
OMAiASPERMG.
OrthoDBiEOG091G17YQ.
PhylomeDBiQ16629.
TreeFamiTF351858.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115875-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72187. mRNA 3'-end processing.
SIGNORiQ16629.

Miscellaneous databases

ChiTaRSiSRSF7. human.
EvolutionaryTraceiQ16629.
GeneWikiiSFRS7.
GenomeRNAii6432.
PROiQ16629.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115875.
CleanExiHS_SFRS7.
ExpressionAtlasiQ16629. baseline and differential.
GenevisibleiQ16629. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF7_HUMAN
AccessioniPrimary (citable) accession number: Q16629
Secondary accession number(s): B4DLU6, G5E9M3, Q564D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 190 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.