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Q16627 (CCL14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 14
Alternative name(s):
Chemokine CC-1/CC-3
Short name=HCC-1/HCC-3
HCC-1(1-74)
NCC-2
Small-inducible cytokine A14

Cleaved into the following 3 chains:

  1. HCC-1(3-74)
  2. HCC-1(4-74)
  3. HCC-1(9-74)
Gene names
Name:CCL14
Synonyms:NCC2, SCYA14
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length93 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Ref.7

Subcellular location

Secreted.

Tissue specificity

Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. Ref.2

Post-translational modification

The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.

HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. Ref.6

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform HCC-1 (identifier: Q16627-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform HCC-3 (identifier: Q16627-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-27: R → QTGGKPKVVKIQLKLVG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.1 Ref.6 Ref.7
Chain20 – 9374C-C motif chemokine 14
PRO_0000005204
Chain22 – 9372HCC-1(3-74)
PRO_0000005205
Chain23 – 9371HCC-1(4-74)
PRO_0000005206
Chain28 – 9366HCC-1(9-74)
PRO_0000005207

Amino acid modifications

Glycosylation261O-linked (GalNAc...); partial Ref.6
Disulfide bond35 ↔ 59
Disulfide bond36 ↔ 75

Natural variations

Alternative sequence271R → QTGGKPKVVKIQLKLVG in isoform HCC-3.
VSP_001060
Natural variant611K → E.
Corresponds to variant rs16971802 [ dbSNP | Ensembl ].
VAR_048707

Secondary structure

.............. 93
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform HCC-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DDDB899DC9148836

FASTA9310,678
        10         20         30         40         50         60 
MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM DYYETNSQCS 

        70         80         90 
KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN 

« Hide

Isoform HCC-3 [UniParc].

Checksum: 73591D5CFF6D3C28
Show »

FASTA10912,297

References

« Hide 'large scale' references
[1]"HCC-1, a novel chemokine from human plasma."
Schulz-Knappe P., Maegert H.-J., Dewald B., Meyer M., Cetin Y., Kubbies M., Tomeczkowski J., Kirchhoff K., Raida M., Adermann K., Kist A., Reinecke M., Sillard R., Pardigol A., Uguccioni M., Baggiolini M., Forssmann W.-G.
J. Exp. Med. 183:295-299(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM HCC-1), PROTEIN SEQUENCE OF 20-93.
Tissue: Bone marrow.
[2]"HCC-2, a human chemokine: gene structure, expression pattern, and biological activity."
Pardigol A., Forssmann U., Zucht H.-D., Loetscher P., Schulz-Knappe P., Baggiolini M., Forssmann W.-G., Maegert H.-J.
Proc. Natl. Acad. Sci. U.S.A. 95:6308-6313(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS HCC-1 AND HCC-3), TISSUE SPECIFICITY.
Tissue: Liver.
[3]"Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, LEC, and RANTES."
Nomiyama H., Fukuda S., Iio M., Tanase S., Miura R., Yoshie O.
J. Interferon Cytokine Res. 19:227-234(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HCC-1).
Tissue: Pancreas and Spleen.
[6]"Posttranslationally processed forms of the human chemokine HCC-1."
Richter R., Schulz-Knappe P., John H., Forssmann W.-G.
Biochemistry 39:10799-10805(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-32, IDENTIFICATION OF HCC-1(3-74) AND HCC-1(4-74), MASS SPECTROMETRY, GLYCOSYLATION AT SER-26.
[7]"Natural proteolytic processing of hemofiltrate CC chemokine 1 generates a potent CC chemokine receptor (CCR)1 and CCR5 agonist with anti-HIV properties."
Detheux M., Staendker L., Vakili J., Muench J., Forssmann U., Adermann K., Poehlmann S., Vassart G., Kirchhoff F., Parmentier M., Forssmann W.-G.
J. Exp. Med. 192:1501-1508(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-48, IDENTIFICATION OF HCC-1(9-74), MASS SPECTROMETRY, FUNCTION.
[8]"Structural and functional characterization of CC chemokine CCL14."
Blain K.Y., Kwiatkowski W., Zhao Q., La Fleur D., Naik C., Chun T.-W., Tsareva T., Kanakaraj P., Laird M.W., Shah R., George L., Sanyal I., Moore P.A., Demeler B., Choe S.
Biochemistry 46:10008-10015(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS) OF 28-93.
+Additional computationally mapped references.

Web resources

Wikipedia

CCL14 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49270 mRNA. Translation: CAA89264.1.
Z70292 mRNA. Translation: CAA94307.1.
Z70293 mRNA. Translation: CAA94309.1.
Z49269 Genomic DNA. Translation: CAA89263.1.
AF088219 Genomic DNA. Translation: AAC63329.1.
AF088219 Genomic DNA. Translation: AAF23982.1.
CH471147 Genomic DNA. Translation: EAW80105.1.
CH471147 Genomic DNA. Translation: EAW80106.1.
CH471147 Genomic DNA. Translation: EAW80107.1.
CH471147 Genomic DNA. Translation: EAW80108.1.
BC038289 mRNA. Translation: AAH38289.1.
BC045165 mRNA. Translation: AAH45165.1.
RefSeqNP_116738.1. NM_032962.4.
NP_116739.1. NM_032963.3.
UniGeneHs.272493.
Hs.740501.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Q8RX-ray1.82E/F/G/H28-93[»]
2Q8TX-ray2.23A/B/C/D20-93[»]
ProteinModelPortalQ16627.
SMRQ16627. Positions 28-93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112261. 5 interactions.
DIPDIP-5861N.
IntActQ16627. 4 interactions.
STRING9606.ENSP00000409197.

PTM databases

PhosphoSiteQ16627.

Polymorphism databases

DMDM2493670.

Proteomic databases

PaxDbQ16627.
PRIDEQ16627.

Protocols and materials databases

DNASU6358.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000394509; ENSP00000378017; ENSG00000213494. [Q16627-1]
ENST00000435911; ENSP00000409197; ENSG00000213494. [Q16627-2]
ENST00000536149; ENSP00000441771; ENSG00000213494. [Q16627-2]
GeneID6358.
KEGGhsa:6358.
UCSCuc010wcq.1. human. [Q16627-2]
uc010wcr.1. human. [Q16627-1]

Organism-specific databases

CTD6358.
GeneCardsGC17M034343.
H-InvDBHIX0039168.
HGNCHGNC:10612. CCL14.
HPACAB004423.
HPA030268.
MIM601392. gene.
neXtProtNX_Q16627.
PharmGKBPA35545.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG84308.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidQ16627.
KOK05514.
OMAVVKIQLK.
TreeFamTF334888.

Gene expression databases

ArrayExpressQ16627.
BgeeQ16627.
CleanExHS_CCL14.
GenevestigatorQ16627.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ16627.
GenomeRNAi6358.
NextBio24696.
PROQ16627.
SOURCESearch...

Entry information

Entry nameCCL14_HUMAN
AccessionPrimary (citable) accession number: Q16627
Secondary accession number(s): E1P649, E1P650, Q13954
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM