Reviewed,
UniProtKB/Swiss-Prot Q16611 (BAK_HUMAN)
Last modified
November 25, 2008.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (8) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Bcl-2 homologous antagonist/killer Alternative name(s): Apoptosis regulator BAK Bcl-2-like 7 protein | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 211 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the a. repressor BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. |
| Subunit structure | Interacts with BCL2A1 By similarity. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). |
| Subcellular location | Membrane; Single-pass membrane proteinPotential. |
| Tissue specificity | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. |
| Domain | Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. |
| Sequence similarities | Belongs to the Bcl-2 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-519866,EBI-519866 | ||
| ACTR2 | P61160 | 1 | EBI-519866,EBI-353580 | |
| AKAP13 | Q12802 | 1 | EBI-519866,EBI-1373806 | |
| BAX | Q07812 | 1 | EBI-519866,EBI-516580 | |
| BCL2 | P10415 | 1 | EBI-519866,EBI-77694 | |
| BCL2L1 | Q07817-1 | 5 | EBI-519866,EBI-287195 | |
| BID | P55957 | 1 | EBI-519866,EBI-519672 | |
| DFFA | O00273 | 1 | EBI-519866,EBI-727171 | |
| FBL | P22087 | 1 | EBI-519866,EBI-358318 | |
| KRT31 | Q15323 | 1 | EBI-519866,EBI-948001 | |
| MCL1 | Q07820 | 3 | EBI-519866,EBI-1003422 | |
| Mcl1 | P97287 | 3 | EBI-519866,EBI-707292 | From a different organism. |
| MYCBP2 | O75592 | 1 | EBI-519866,EBI-1043774 | |
| SRRM2 | Q9UQ35 | 1 | EBI-519866,EBI-1050142 | |
| VDAC2 | P45880 | 1 | EBI-519866,EBI-354022 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 211 | 211 | Bcl-2 homologous antagonist/killer | PRO_0000143059 | |||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||
| Transmembrane | 188 – 205 | 18 | Potential | ||||||||||||||||||||||||||
| Motif | 74 – 88 | 15 | BH3 | ||||||||||||||||||||||||||
| Motif | 117 – 136 | 20 | BH1 | ||||||||||||||||||||||||||
| Motif | 169 – 184 | 16 | BH2 | ||||||||||||||||||||||||||
Sites | |||||||||||||||||||||||||||||
| Metal binding | 160 | 1 | Zinc; shared with dimeric partner | ||||||||||||||||||||||||||
| Metal binding | 164 | 1 | Zinc; shared with dimeric partner | ||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||
| Natural variant | 28 | 1 | A → V: dbSNP rs4987115. | VAR_018829 | |||||||||||||||||||||||||
| Natural variant | 69 | 1 | S → R: dbSNP rs5745592. | VAR_018830 | |||||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||||||
| Mutagenesis | 164 | 1 | H → A: Strongly reduced zinc binding and homodimerization | ||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Helix | 24 – 50 | 27 | |||||||||||||||||||||||||||
| Helix | 51 – 53 | 3 | |||||||||||||||||||||||||||
| Helix | 59 – 62 | 4 | |||||||||||||||||||||||||||
| Helix | 70 – 85 | 16 | |||||||||||||||||||||||||||
| Helix | 87 – 97 | 11 | |||||||||||||||||||||||||||
| Helix | 104 – 118 | 15 | |||||||||||||||||||||||||||
| Turn | 119 – 121 | 3 | |||||||||||||||||||||||||||
| Helix | 125 – 144 | 20 | |||||||||||||||||||||||||||
| Helix | 151 – 164 | 14 | |||||||||||||||||||||||||||
| Helix | 167 – 173 | 7 | |||||||||||||||||||||||||||
| Helix | 177 – 180 | 4 | |||||||||||||||||||||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K." Farrow S.N., White J.H.M., Martinou I., Raven T., Pun K.-T., Grinham C.J., Martinou J.-C., Brown R. Nature 374:731-733(1995) [PubMed: 7715729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell. |
| [2] | "Induction of apoptosis by the Bcl-2 homologue Bak." Chittenden T., Harrington E.A., O'Connor R., Flemington C., Lutz R.J., Evan G.I., Guild B.C. Nature 374:733-736(1995) [PubMed: 7715730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." Kiefer M.C., Brauer M.J., Powers V.C., Wu J.J., Umansky S.R., Tomei L.D., Barr P.J. Nature 374:736-739(1995) [PubMed: 7715731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Rieder M.J., Livingston R.J., Daniels M.R., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-28 AND ARG-69. |
| [5] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung. |
| [7] | "Estrogen alters expression of apoptosis-regulators, Bcl-2, Bcl-xL and Bak, as well as susceptibility to therapeutic agents of human breast cancer cells." Eguchi H., Hayashi S. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 96-206. |
| [8] | "A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions." Chittenden T., Flemington C., Houghton A.B., Ebb R.G., Gallo G.J., Elangovan B., Chinnadurai G., Lutz R.J. EMBO J. 14:5589-5596(1995) [PubMed: 8521816] [Abstract] Cited for: MUTAGENESIS, FUNCTION OF BH3 MOTIF. |
| [9] | "Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis." Sattler M., Liang H., Nettesheim D., Meadows R.P., Harlan J.E., Eberstadt M., Yoon H.S., Shuker S.B., Chang B.S., Minn A.J., Thompson C.B., Fesik S.W. Science 275:983-986(1997) [PubMed: 9020082] [Abstract] Cited for: STRUCTURE BY NMR OF 72-87 IN COMPLEX WITH BCL2L1 ISOFORM BCL-X(L). |
| [10] | "The X-ray structure of a BAK homodimer reveals an inhibitory zinc binding site." Moldoveanu T., Liu Q., Tocilj A., Watson M., Shore G., Gehring K. Mol. Cell 24:677-688(2006) [PubMed: 17157251] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.48 ANGSTROMS) OF 16-186, FUNCTION, SUBUNIT, MUTAGENESIS OF HIS-164, ZINC BINDING. |
| [11] | "Crystal structure of MS0836." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X84213 mRNA. Translation: CAA58997.1. U23765 mRNA. Translation: AAA93066.1. U16811 mRNA. Translation: AAA74466.1. AY260471 Genomic DNA. Translation: AAO74828.1. Z93017 Genomic DNA. Translation: CAB65626.1. BC004431 mRNA. Translation: AAH04431.1. D88397 Genomic DNA. Translation: BAA13606.1. | |||||||||||||||||||||||||||||||||||||
| PIR | S58873. | ||||||||||||||||||||||||||||||||||||
| RefSeq | NP_001179.1. | ||||||||||||||||||||||||||||||||||||
| UniGene | Hs.485139 | ||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||
| DIP | DIP:935N. | ||||||||||||||||||||||||||||||||||||
| IntAct | Q16611. | ||||||||||||||||||||||||||||||||||||
Polymorphism databases | |||||||||||||||||||||||||||||||||||||
| NIEHS-SNPs | Search... | ||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||
| Ensembl | ENSG00000030110. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||||||||
| GeneID | 578. | ||||||||||||||||||||||||||||||||||||
| KEGG | hsa:578. | ||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||
| H-InvDB | HIX0005780. HIX0040824. HIX0058969. | ||||||||||||||||||||||||||||||||||||
| HGNC | HGNC:949. BAK1. | ||||||||||||||||||||||||||||||||||||
| HPA | CAB005029. | ||||||||||||||||||||||||||||||||||||
| MIM | 600516. gene. | ||||||||||||||||||||||||||||||||||||
| PharmGKB | PA25253. | ||||||||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||
| HOVERGEN | Q16611. | ||||||||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||||||||
| Reactome | REACT_578. Apoptosis. | ||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||
| CleanEx | HS_BAK1. | ||||||||||||||||||||||||||||||||||||
| GermOnline | ENSG00000030110. Homo sapiens. | ||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||
| InterPro | IPR002475. BCL2_apoptsis. IPR000712. Bcl2_BH. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| Pfam | PF00452. Bcl-2. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| PRINTS | PR01862. BCL2FAMILY. | ||||||||||||||||||||||||||||||||||||
| SMART | SM00337. BCL. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| PROSITE | PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01259. BH3. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||
| LinkHub | Q16611. | ||||||||||||||||||||||||||||||||||||
| NextBio | 2359. | ||||||||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | BAK_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q16611 Secondary accession number(s): Q92533 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |
| Forthcoming format changes Announcement of forthcoming format changes |

Clusters with


