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Protein

Pseudouridine-5'-phosphate glycosidase

Gene

psuG

Organism
Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.UniRule annotation

Catalytic activityi

Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei26Proton donorUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei107Substrate; via amide nitrogenUniRule annotation1
Metal bindingi139ManganeseUniRule annotation1
Active sitei160NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudouridine-5'-phosphate glycosidaseUniRule annotation (EC:4.2.1.70UniRule annotation)
Short name:
PsiMP glycosidaseUniRule annotation
Gene namesi
Name:psuGUniRule annotation
Ordered Locus Names:RD1_2734
OrganismiRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)
Taxonomic identifieri375451 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter
Proteomesi
  • UP000007029 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003905421 – 310Pseudouridine-5'-phosphate glycosidaseAdd BLAST310

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi375451.RD1_2734.

Structurei

3D structure databases

ProteinModelPortaliQ165S4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 143Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the pseudouridine-5'-phosphate glycosidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D53. Bacteria.
COG2313. LUCA.
HOGENOMiHOG000064311.
KOiK16329.
OMAiIIAHGMP.
OrthoDBiPOG091H08AG.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
HAMAPiMF_01876. PsiMP_glycosidase. 1 hit.
InterProiIPR007342. PsuG.
IPR029061. THDP-binding.
[Graphical view]
PANTHERiPTHR10584:SF1. PTHR10584:SF1. 1 hit.
PfamiPF04227. Indigoidine_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q165S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLPLRHSA EVAAAKAAGR PIVALESTII THGMPYPQNL EVARQVEDDL
60 70 80 90 100
RAAGVTPATI AVMDGTLHIG LEAHQLEALA QAKGVAKLSR ADIAACMATG
110 120 130 140 150
GTGATTVSAT MIAAHLAGIS VFATGGIGGV HRGAESSFDV SADLLELAQT
160 170 180 190 200
PVTVVAAGAK AILDVAKTLE VLETQGVPVI TVGQDSFPAF WSAESVFKSP
210 220 230 240 250
LRMDDPVGIA AAHQMRVDLG LPGGQLVANP IPHTDEIPAT ELAPIIATAQ
260 270 280 290 300
ADAEKHGIKG KAVTPYLLQR IYELTQGRSL TANIALVRNN ARLAGAIAQA
310
LIANARIAGK
Length:310
Mass (Da):31,819
Last modified:July 25, 2006 - v1
Checksum:i6C6C378C7A65A249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG32269.1.
RefSeqiWP_011568886.1. NC_008209.1.

Genome annotation databases

EnsemblBacteriaiABG32269; ABG32269; RD1_2734.
KEGGirde:RD1_2734.
PATRICi23363467. VBIRosDen86677_2609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000362 Genomic DNA. Translation: ABG32269.1.
RefSeqiWP_011568886.1. NC_008209.1.

3D structure databases

ProteinModelPortaliQ165S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi375451.RD1_2734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG32269; ABG32269; RD1_2734.
KEGGirde:RD1_2734.
PATRICi23363467. VBIRosDen86677_2609.

Phylogenomic databases

eggNOGiENOG4105D53. Bacteria.
COG2313. LUCA.
HOGENOMiHOG000064311.
KOiK16329.
OMAiIIAHGMP.
OrthoDBiPOG091H08AG.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
HAMAPiMF_01876. PsiMP_glycosidase. 1 hit.
InterProiIPR007342. PsuG.
IPR029061. THDP-binding.
[Graphical view]
PANTHERiPTHR10584:SF1. PTHR10584:SF1. 1 hit.
PfamiPF04227. Indigoidine_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSUG_ROSDO
AccessioniPrimary (citable) accession number: Q165S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: July 25, 2006
Last modified: November 30, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.