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Q165E2 (GLGB_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:RD1_2875
OrganismRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier375451 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7317311,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260693

Sites

Active site4091Nucleophile By similarity
Active site4621Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q165E2 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 59845556CC367F52

FASTA73181,701
        10         20         30         40         50         60 
MNISQTDAEK LAAGCHGNPF SVLGLHEVAG KLSLRVFLPG AETVEALDPK TGRVIVTLNQ 

        70         80         90        100        110        120 
TETPGLFEGT AARRKKRFAY HLRITQGAHQ WQMDDPYRFG PVIGDIDEYL LGEGSHRRLW 

       130        140        150        160        170        180 
SVLGAHVITH EGVAGTHFAV WAPNAQRVSV VGEFNVWDGR RMMMRQRGAT GVWEIFVPGI 

       190        200        210        220        230        240 
GEGEIYKYEI IAPDGSLLPQ KADPVGFGAE HPPRTGSVVR KIDGYAWDDA QWMTRRADVQ 

       250        260        270        280        290        300 
RIDQPVSIYE VHLGSWRRVP EDGNRPLSYV ELADQLVAYV KDMGFTHMEV MPVSEFPFDG 

       310        320        330        340        350        360 
SWGYQPIGLF APTIRHGTLD EFRALVAACH RENIGVILDW VPGHFPEDAH GLGRFDGTAL 

       370        380        390        400        410        420 
YEHADRKEGF HPDWNTLVYN YGRAEVANYL AANALYWLQE HHVDGLRVDA VASMLYRDYS 

       430        440        450        460        470        480 
RKDGEWIPNR DGGRENYEAI SFLQKVNTES YGEVPGIMTI AEESTAFPGV SAPVDAGGLG 

       490        500        510        520        530        540 
FGFKWNMGWM NDTLQYMQQD PIHRKYHHHE MTFGLHYAFS ENFILPLSHD EVVHGKGSLL 

       550        560        570        580        590        600 
DKMPGQGDDK FANLRAYYGF MWGHPGKKLL FMGCEFAQGV EWNHDSSLDW HLLDHPQHRG 

       610        620        630        640        650        660 
MQNLVRDLNA LYRENAALHG LDCAAQGFEW IDENNAEASV LAWLRHGPDG GAPMLVVSNF 

       670        680        690        700        710        720 
TPVERSHYRL GVPQAGRWVE RLNTNADCYG GTGLGNLGGV QSADIAAAGR PFSIDVVLPP 

       730 
LTTLFFQLDD G 

« Hide

References

[1]"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism."
Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W.
J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000362 Genomic DNA. Translation: ABG32401.1.
RefSeqYP_683087.1. NC_008209.1.

3D structure databases

ProteinModelPortalQ165E2.
SMRQ165E2. Positions 115-729.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING375451.RD1_2875.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG32401; ABG32401; RD1_2875.
GeneID4196033.
KEGGrde:RD1_2875.
PATRIC23363741. VBIRosDen86677_2743.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAPIGVAYK.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRDEN375451:GJIZ-2707-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_ROSDO
AccessionPrimary (citable) accession number: Q165E2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries