Q16581 (C3AR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C3a anaphylatoxin chemotactic receptor
Short name=C3AR
Short name=C3a-R

Gene names

Name:	C3AR1
Synonyms:	AZ3B, C3R1, HNFAG09

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
Post-translational modification	Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin. O-glycosylated. Ref.10
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood circulation Traceable author statement Ref.2. Source: ProtInc chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW elevation of cytosolic calcium ion concentration Inferred from genetic interaction PubMed 10571060. Source: UniProtKB inflammatory response Traceable author statement Ref.2. Source: ProtInc positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of macrophage chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation vascular endothelial growth factor production Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.4. Source: ProtInc
Molecular_function	C3a anaphylatoxin receptor activity Traceable author statement Ref.4. Source: ProtInc N-formyl peptide receptor activity Inferred from electronic annotation. Source: InterPro complement component C3a receptor activity Traceable author statement Ref.2. Source: ProtInc phosphatidylinositol phospholipase C activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	C3a anaphylatoxin chemotactic receptor		PRO_0000069202
Regions
	Topological domain	1 – 23	23	Extracellular Potential
	Transmembrane	24 – 46	23	Helical; Name=1; Potential
	Topological domain	47 – 57	11	Cytoplasmic Potential
	Transmembrane	58 – 80	23	Helical; Name=2; Potential
	Topological domain	81 – 96	16	Extracellular Potential
	Transmembrane	97 – 118	22	Helical; Name=3; Potential
	Topological domain	119 – 139	21	Cytoplasmic Potential
	Transmembrane	140 – 160	21	Helical; Name=4; Potential
	Topological domain	161 – 340	180	Extracellular Potential
	Transmembrane	341 – 360	20	Helical; Name=5; Potential
	Topological domain	361 – 377	17	Cytoplasmic Potential
	Transmembrane	378 – 400	23	Helical; Name=6; Potential
	Topological domain	401 – 417	17	Extracellular Potential
	Transmembrane	418 – 438	21	Helical; Name=7; Potential
	Topological domain	439 – 482	44	Cytoplasmic Potential
Amino acid modifications
	Modified residue	174	1	Sulfotyrosine Ref.9
	Modified residue	184	1	Sulfotyrosine Ref.9
	Modified residue	318	1	Sulfotyrosine Ref.9
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	194	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	O-linked (GalNAc...) Ref.10
	Disulfide bond	95 ↔ 172		By similarity
Natural variations
	Natural variant	136	1	V → A. Ref.7 Corresponds to variant rs11567806 [ dbSNP | Ensembl ].		VAR_019164
Experimental info
	Sequence conflict	151	1	F → C in AAC50657. Ref.4
	Sequence conflict	203	1	P → R in AAC50374. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q16581 [UniParc]. Last modified October 3, 2003. Version 2. Checksum: 287E219D98CED203	FASTA	482	53,864
	10 20 30 40 50 60 MASFSAETNS TDLLSQPWNE PPVILSMVIL SLTFLLGLPG NGLVLWVAGL KMQRTVNTIW 70 80 90 100 110 120 FLHLTLADLL CCLSLPFSLA HLALQGQWPY GRFLCKLIPS IIVLNMFASV FLLTAISLDR 130 140 150 160 170 180 CLVVFKPIWC QNHRNVGMAC SICGCIWVVA FVMCIPVFVY REIFTTDNHN RCGYKFGLSS 190 200 210 220 230 240 SLDYPDFYGD PLENRSLENI VQPPGEMNDR LDPSSFQTND HPWTVPTVFQ PQTFQRPSAD 250 260 270 280 290 300 SLPRGSARLT SQNLYSNVFK PADVVSPKIP SGFPIEDHET SPLDNSDAFL STHLKLFPSA 310 320 330 340 350 360 SSNSFYESEL PQGFQDYYNL GQFTDDDQVP TPLVAITITR LVVGFLLPSV IMIACYSFIV 370 380 390 400 410 420 FRMQRGRFAK SQSKTFRVAV VVVAVFLVCW TPYHIFGVLS LLTDPETPLG KTLMSWDHVC 430 440 450 460 470 480 IALASANSCF NPFLYALLGK DFRKKARQSI QGILEAAFSE ELTRSTHCPS NNVISERNST TV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of a novel G protein-coupled receptor with a large extracellular loop structure." Roglic A., Prossnitz E.R., Cavanagh S.L., Pan Z., Zou A., Ye R.D. Biochim. Biophys. Acta 1305:39-43(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells." Crass T., Raffetseder U., Martin U., Grove M., Klos A., Koehl J., Bautsch W. Eur. J. Immunol. 26:1944-1950(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Bautsch W. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Molecular cloning and characterization of the human anaphylatoxin C3a receptor." Ames R.S., Li Y., Sarau H.M., Nuthulaganti P., Foley J.J., Ellis C., Zeng Z., Su K., Jurewicz A.J., Hertzberg R.P., Bergsma D.J., Kumar C. J. Biol. Chem. 271:20231-20234(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes." Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	SeattleSNPs variation discovery resource Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-136.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[9]	"Sulfation of tyrosine 174 in the human C3a receptor is essential for binding of C3a anaphylatoxin." Gao J., Choe H., Bota D., Wright P.L., Gerard C., Gerard N.P. J. Biol. Chem. 278:37902-37908(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SULFATION AT TYR-174; TYR-184 AND TYR-318.
[10]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT SER-266, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28488 mRNA. Translation: AAC50374.1. Z73157 mRNA. Translation: CAA97504.1. U62027 mRNA. Translation: AAC50657.1. AB065870 Genomic DNA. Translation: BAC06088.1. AY268431 Genomic DNA. Translation: AAP23198.1. AY455929 Genomic DNA. Translation: AAR13862.1. BC020742 mRNA. Translation: AAH20742.1.
IPI	IPI00293530.
PIR	S65766.
RefSeq	NP_004045.1. NM_004054.2.
UniGene	Hs.591148.
3D structure databases
ProteinModelPortal	Q16581.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000302079.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q16581.
Polymorphism databases
DMDM	37538305.
Proteomic databases
PaxDb	Q16581.
PRIDE	Q16581.
Protocols and materials databases
DNASU	719.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307637; ENSP00000302079; ENSG00000171860.
GeneID	719.
KEGG	hsa:719.
UCSC	uc001qtv.1. human.
Organism-specific databases
CTD	719.
GeneCards	GC12M008210.
HGNC	HGNC:1319. C3AR1.
HPA	CAB009405.
MIM	605246. gene.
neXtProt	NX_Q16581.
PharmGKB	PA25898.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148047.
HOGENOM	HOG000261616.
HOVERGEN	HBG002433.
InParanoid	Q16581.
KO	K04009.
OMA	LWVAGLK.
OrthoDB	EOG4PC9S8.
PhylomeDB	Q16581.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q16581.
Bgee	Q16581.
CleanEx	HS_C3AR1.
Genevestigator	Q16581.
GermOnline	ENSG00000171860. Homo sapiens.
Family and domain databases
InterPro	IPR002234. Anphylx_rcpt. IPR001644. C3A_anaphtx_rcpt. IPR000826. Formyl_pep_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24225. PTHR24225. 1 hit.
Pfam	PF00001. 7tm_1. 2 hits. [Graphical view]
PRINTS	PR01104. ANPHYLATOXNR. PR01060. C3ANPHYLTXNR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q16581.
ChEMBL	CHEMBL4761.
GenomeRNAi	719.
NextBio	2926.
SOURCE	Search...

Entry information

Entry name

C3AR_HUMAN

Accession

Primary (citable) accession number: Q16581
Secondary accession number(s): O43771, Q92868

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 3, 2003
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.