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Protein

C3a anaphylatoxin chemotactic receptor

Gene

C3AR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

GO - Molecular functioni

  • C3a anaphylatoxin receptor activity Source: ProtInc
  • complement component C3a receptor activity Source: ProtInc
  • G-protein coupled receptor activity Source: UniProtKB
  • phosphatidylinositol phospholipase C activity Source: ProtInc

GO - Biological processi

  • blood circulation Source: ProtInc
  • chemotaxis Source: UniProtKB-KW
  • complement receptor mediated signaling pathway Source: GOC
  • G-protein coupled receptor signaling pathway Source: ProtInc
  • inflammatory response Source: ProtInc
  • metabolic process Source: GOC
  • positive regulation of angiogenesis Source: Ensembl
  • positive regulation of cytosolic calcium ion concentration Source: UniProtKB
  • positive regulation of macrophage chemotaxis Source: Ensembl
  • positive regulation of neutrophil chemotaxis Source: Ensembl
  • positive regulation of vascular endothelial growth factor production Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C3a anaphylatoxin chemotactic receptor
Short name:
C3AR
Short name:
C3a-R
Gene namesi
Name:C3AR1
Synonyms:AZ3B, C3R1, HNFAG09
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:1319. C3AR1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323ExtracellularSequence AnalysisAdd
BLAST
Transmembranei24 – 4623Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini47 – 5711CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei58 – 8023Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini81 – 9616ExtracellularSequence AnalysisAdd
BLAST
Transmembranei97 – 11822Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini119 – 13921CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei140 – 16021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini161 – 340180ExtracellularSequence AnalysisAdd
BLAST
Transmembranei341 – 36020Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini361 – 37717CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei378 – 40023Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini401 – 41717ExtracellularSequence AnalysisAdd
BLAST
Transmembranei418 – 43821Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini439 – 48244CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: GOC
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25898.

Polymorphism and mutation databases

BioMutaiC3AR1.
DMDMi37538305.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482C3a anaphylatoxin chemotactic receptorPRO_0000069202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi9 – 91N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi95 ↔ 172PROSITE-ProRule annotation
Modified residuei174 – 1741Sulfotyrosine1 Publication
Modified residuei184 – 1841Sulfotyrosine1 Publication
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi266 – 2661O-linked (GalNAc...)1 Publication
Modified residuei318 – 3181Sulfotyrosine1 Publication

Post-translational modificationi

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.1 Publication
O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiQ16581.
PRIDEiQ16581.

PTM databases

PhosphoSiteiQ16581.

Expressioni

Tissue specificityi

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Gene expression databases

BgeeiQ16581.
CleanExiHS_C3AR1.
ExpressionAtlasiQ16581. baseline and differential.
GenevisibleiQ16581. HS.

Organism-specific databases

HPAiCAB009405.

Interactioni

Protein-protein interaction databases

BioGridi107180. 1 interaction.
STRINGi9606.ENSP00000302079.

Structurei

3D structure databases

ProteinModelPortaliQ16581.
SMRiQ16581. Positions 22-188, 310-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148047.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000261616.
HOVERGENiHBG002433.
InParanoidiQ16581.
KOiK04009.
OMAiLWVAGLK.
OrthoDBiEOG7PGDQQ.
PhylomeDBiQ16581.
TreeFamiTF330976.

Family and domain databases

InterProiIPR001644. Anaphtx_C3AR1.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF4. PTHR24225:SF4. 1 hit.
PfamiPF00001. 7tm_1. 2 hits.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR01060. C3ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16581-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFSAETNS TDLLSQPWNE PPVILSMVIL SLTFLLGLPG NGLVLWVAGL
60 70 80 90 100
KMQRTVNTIW FLHLTLADLL CCLSLPFSLA HLALQGQWPY GRFLCKLIPS
110 120 130 140 150
IIVLNMFASV FLLTAISLDR CLVVFKPIWC QNHRNVGMAC SICGCIWVVA
160 170 180 190 200
FVMCIPVFVY REIFTTDNHN RCGYKFGLSS SLDYPDFYGD PLENRSLENI
210 220 230 240 250
VQPPGEMNDR LDPSSFQTND HPWTVPTVFQ PQTFQRPSAD SLPRGSARLT
260 270 280 290 300
SQNLYSNVFK PADVVSPKIP SGFPIEDHET SPLDNSDAFL STHLKLFPSA
310 320 330 340 350
SSNSFYESEL PQGFQDYYNL GQFTDDDQVP TPLVAITITR LVVGFLLPSV
360 370 380 390 400
IMIACYSFIV FRMQRGRFAK SQSKTFRVAV VVVAVFLVCW TPYHIFGVLS
410 420 430 440 450
LLTDPETPLG KTLMSWDHVC IALASANSCF NPFLYALLGK DFRKKARQSI
460 470 480
QGILEAAFSE ELTRSTHCPS NNVISERNST TV
Length:482
Mass (Da):53,864
Last modified:October 3, 2003 - v2
Checksum:i287E219D98CED203
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511F → C in AAC50657 (PubMed:8702752).Curated
Sequence conflicti203 – 2031P → R in AAC50374 (PubMed:8605247).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti136 – 1361V → A.1 Publication
Corresponds to variant rs11567806 [ dbSNP | Ensembl ].
VAR_019164

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28488 mRNA. Translation: AAC50374.1.
Z73157 mRNA. Translation: CAA97504.1.
U62027 mRNA. Translation: AAC50657.1.
AB065870 Genomic DNA. Translation: BAC06088.1.
AY268431 Genomic DNA. Translation: AAP23198.1.
AY455929 Genomic DNA. Translation: AAR13862.1.
BC020742 mRNA. Translation: AAH20742.1.
CCDSiCCDS8588.1.
PIRiS65766.
RefSeqiNP_004045.1. NM_004054.2.
UniGeneiHs.591148.

Genome annotation databases

EnsembliENST00000307637; ENSP00000302079; ENSG00000171860.
GeneIDi719.
KEGGihsa:719.
UCSCiuc001qtv.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28488 mRNA. Translation: AAC50374.1.
Z73157 mRNA. Translation: CAA97504.1.
U62027 mRNA. Translation: AAC50657.1.
AB065870 Genomic DNA. Translation: BAC06088.1.
AY268431 Genomic DNA. Translation: AAP23198.1.
AY455929 Genomic DNA. Translation: AAR13862.1.
BC020742 mRNA. Translation: AAH20742.1.
CCDSiCCDS8588.1.
PIRiS65766.
RefSeqiNP_004045.1. NM_004054.2.
UniGeneiHs.591148.

3D structure databases

ProteinModelPortaliQ16581.
SMRiQ16581. Positions 22-188, 310-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107180. 1 interaction.
STRINGi9606.ENSP00000302079.

Chemistry

BindingDBiQ16581.
ChEMBLiCHEMBL4761.
GuidetoPHARMACOLOGYi31.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ16581.

Polymorphism and mutation databases

BioMutaiC3AR1.
DMDMi37538305.

Proteomic databases

PaxDbiQ16581.
PRIDEiQ16581.

Protocols and materials databases

DNASUi719.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307637; ENSP00000302079; ENSG00000171860.
GeneIDi719.
KEGGihsa:719.
UCSCiuc001qtv.1. human.

Organism-specific databases

CTDi719.
GeneCardsiGC12M008210.
HGNCiHGNC:1319. C3AR1.
HPAiCAB009405.
MIMi605246. gene.
neXtProtiNX_Q16581.
PharmGKBiPA25898.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG148047.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000261616.
HOVERGENiHBG002433.
InParanoidiQ16581.
KOiK04009.
OMAiLWVAGLK.
OrthoDBiEOG7PGDQQ.
PhylomeDBiQ16581.
TreeFamiTF330976.

Enzyme and pathway databases

ReactomeiREACT_14819. Peptide ligand-binding receptors.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiC3AR1. human.
GeneWikiiC3a_receptor.
GenomeRNAii719.
NextBioi2926.
PROiQ16581.
SOURCEiSearch...

Gene expression databases

BgeeiQ16581.
CleanExiHS_C3AR1.
ExpressionAtlasiQ16581. baseline and differential.
GenevisibleiQ16581. HS.

Family and domain databases

InterProiIPR001644. Anaphtx_C3AR1.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF4. PTHR24225:SF4. 1 hit.
PfamiPF00001. 7tm_1. 2 hits.
[Graphical view]
PRINTSiPR01104. ANPHYLATOXNR.
PR01060. C3ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of a novel G protein-coupled receptor with a large extracellular loop structure."
    Roglic A., Prossnitz E.R., Cavanagh S.L., Pan Z., Zou A., Ye R.D.
    Biochim. Biophys. Acta 1305:39-43(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells."
    Crass T., Raffetseder U., Martin U., Grove M., Klos A., Koehl J., Bautsch W.
    Eur. J. Immunol. 26:1944-1950(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Bautsch W.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
    Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Kopatz S.A., Aronstam R.S., Sharma S.V.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. SeattleSNPs variation discovery resource
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-136.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  9. "Sulfation of tyrosine 174 in the human C3a receptor is essential for binding of C3a anaphylatoxin."
    Gao J., Choe H., Bota D., Wright P.L., Gerard C., Gerard N.P.
    J. Biol. Chem. 278:37902-37908(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SULFATION AT TYR-174; TYR-184 AND TYR-318.
  10. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiC3AR_HUMAN
AccessioniPrimary (citable) accession number: Q16581
Secondary accession number(s): O43771, Q92868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2003
Last modified: June 24, 2015
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.