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Q16581 (C3AR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C3a anaphylatoxin chemotactic receptor

Short name=C3AR
Short name=C3a-R
Gene names
Name:C3AR1
Synonyms:AZ3B, C3R1, HNFAG09
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Post-translational modification

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

O-glycosylated. Ref.10

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 8898085. Source: ProtInc

blood circulation

Traceable author statement Ref.2. Source: ProtInc

chemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

complement receptor mediated signaling pathway

Traceable author statement Ref.2. Source: GOC

inflammatory response

Traceable author statement Ref.2. Source: ProtInc

metabolic process

Traceable author statement Ref.2. Source: GOC

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from genetic interaction PubMed 10571060. Source: UniProtKB

positive regulation of macrophage chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of neutrophil chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation vascular endothelial growth factor production

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.4. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionC3a anaphylatoxin receptor activity

Traceable author statement Ref.4. Source: ProtInc

G-protein coupled receptor activity

Inferred from genetic interaction PubMed 10571060. Source: UniProtKB

N-formyl peptide receptor activity

Inferred from electronic annotation. Source: InterPro

complement component C3a receptor activity

Traceable author statement Ref.2. Source: ProtInc

phosphatidylinositol phospholipase C activity

Traceable author statement Ref.2. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482C3a anaphylatoxin chemotactic receptor
PRO_0000069202

Regions

Topological domain1 – 2323Extracellular Potential
Transmembrane24 – 4623Helical; Name=1; Potential
Topological domain47 – 5711Cytoplasmic Potential
Transmembrane58 – 8023Helical; Name=2; Potential
Topological domain81 – 9616Extracellular Potential
Transmembrane97 – 11822Helical; Name=3; Potential
Topological domain119 – 13921Cytoplasmic Potential
Transmembrane140 – 16021Helical; Name=4; Potential
Topological domain161 – 340180Extracellular Potential
Transmembrane341 – 36020Helical; Name=5; Potential
Topological domain361 – 37717Cytoplasmic Potential
Transmembrane378 – 40023Helical; Name=6; Potential
Topological domain401 – 41717Extracellular Potential
Transmembrane418 – 43821Helical; Name=7; Potential
Topological domain439 – 48244Cytoplasmic Potential

Amino acid modifications

Modified residue1741Sulfotyrosine Ref.9
Modified residue1841Sulfotyrosine Ref.9
Modified residue3181Sulfotyrosine Ref.9
Glycosylation91N-linked (GlcNAc...) Potential
Glycosylation1941N-linked (GlcNAc...) Potential
Glycosylation2661O-linked (GalNAc...) Ref.10
Disulfide bond95 ↔ 172 By similarity

Natural variations

Natural variant1361V → A. Ref.7
Corresponds to variant rs11567806 [ dbSNP | Ensembl ].
VAR_019164

Experimental info

Sequence conflict1511F → C in AAC50657. Ref.4
Sequence conflict2031P → R in AAC50374. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q16581 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: 287E219D98CED203

FASTA48253,864
        10         20         30         40         50         60 
MASFSAETNS TDLLSQPWNE PPVILSMVIL SLTFLLGLPG NGLVLWVAGL KMQRTVNTIW 

        70         80         90        100        110        120 
FLHLTLADLL CCLSLPFSLA HLALQGQWPY GRFLCKLIPS IIVLNMFASV FLLTAISLDR 

       130        140        150        160        170        180 
CLVVFKPIWC QNHRNVGMAC SICGCIWVVA FVMCIPVFVY REIFTTDNHN RCGYKFGLSS 

       190        200        210        220        230        240 
SLDYPDFYGD PLENRSLENI VQPPGEMNDR LDPSSFQTND HPWTVPTVFQ PQTFQRPSAD 

       250        260        270        280        290        300 
SLPRGSARLT SQNLYSNVFK PADVVSPKIP SGFPIEDHET SPLDNSDAFL STHLKLFPSA 

       310        320        330        340        350        360 
SSNSFYESEL PQGFQDYYNL GQFTDDDQVP TPLVAITITR LVVGFLLPSV IMIACYSFIV 

       370        380        390        400        410        420 
FRMQRGRFAK SQSKTFRVAV VVVAVFLVCW TPYHIFGVLS LLTDPETPLG KTLMSWDHVC 

       430        440        450        460        470        480 
IALASANSCF NPFLYALLGK DFRKKARQSI QGILEAAFSE ELTRSTHCPS NNVISERNST 


TV 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of a novel G protein-coupled receptor with a large extracellular loop structure."
Roglic A., Prossnitz E.R., Cavanagh S.L., Pan Z., Zou A., Ye R.D.
Biochim. Biophys. Acta 1305:39-43(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Expression cloning of the human C3a anaphylatoxin receptor (C3aR) from differentiated U-937 cells."
Crass T., Raffetseder U., Martin U., Grove M., Klos A., Koehl J., Bautsch W.
Eur. J. Immunol. 26:1944-1950(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]Bautsch W.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"Molecular cloning and characterization of the human anaphylatoxin C3a receptor."
Ames R.S., Li Y., Sarau H.M., Nuthulaganti P., Foley J.J., Ellis C., Zeng Z., Su K., Jurewicz A.J., Hertzberg R.P., Bergsma D.J., Kumar C.
J. Biol. Chem. 271:20231-20234(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]SeattleSNPs variation discovery resource
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-136.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[9]"Sulfation of tyrosine 174 in the human C3a receptor is essential for binding of C3a anaphylatoxin."
Gao J., Choe H., Bota D., Wright P.L., Gerard C., Gerard N.P.
J. Biol. Chem. 278:37902-37908(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SULFATION AT TYR-174; TYR-184 AND TYR-318.
[10]"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
Halim A., Ruetschi U., Larson G., Nilsson J.
J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28488 mRNA. Translation: AAC50374.1.
Z73157 mRNA. Translation: CAA97504.1.
U62027 mRNA. Translation: AAC50657.1.
AB065870 Genomic DNA. Translation: BAC06088.1.
AY268431 Genomic DNA. Translation: AAP23198.1.
AY455929 Genomic DNA. Translation: AAR13862.1.
BC020742 mRNA. Translation: AAH20742.1.
CCDSCCDS8588.1.
PIRS65766.
RefSeqNP_004045.1. NM_004054.2.
UniGeneHs.591148.

3D structure databases

ProteinModelPortalQ16581.
SMRQ16581. Positions 24-452.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107180. 1 interaction.
STRING9606.ENSP00000302079.

Chemistry

BindingDBQ16581.
ChEMBLCHEMBL4761.
GuidetoPHARMACOLOGY31.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ16581.

Polymorphism databases

DMDM37538305.

Proteomic databases

PaxDbQ16581.
PRIDEQ16581.

Protocols and materials databases

DNASU719.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307637; ENSP00000302079; ENSG00000171860.
GeneID719.
KEGGhsa:719.
UCSCuc001qtv.1. human.

Organism-specific databases

CTD719.
GeneCardsGC12M008210.
HGNCHGNC:1319. C3AR1.
HPACAB009405.
MIM605246. gene.
neXtProtNX_Q16581.
PharmGKBPA25898.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148047.
HOGENOMHOG000261616.
HOVERGENHBG002433.
InParanoidQ16581.
KOK04009.
OMALWVAGLK.
OrthoDBEOG7PGDQQ.
PhylomeDBQ16581.
TreeFamTF330976.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ16581.
BgeeQ16581.
CleanExHS_C3AR1.
GenevestigatorQ16581.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR001644. Anaphtx_C3AR1.
IPR002234. Anphylx_rcpt.
IPR000826. Formyl_rcpt-rel.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF4. PTHR24225:SF4. 1 hit.
PfamPF00001. 7tm_1. 2 hits.
[Graphical view]
PRINTSPR01104. ANPHYLATOXNR.
PR01060. C3ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiC3a_receptor.
GenomeRNAi719.
NextBio2926.
PROQ16581.
SOURCESearch...

Entry information

Entry nameC3AR_HUMAN
AccessionPrimary (citable) accession number: Q16581
Secondary accession number(s): O43771, Q92868
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 3, 2003
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries