ID VACHT_HUMAN Reviewed; 532 AA. AC Q16572; B2R7S1; DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot. DT 30-NOV-2010, sequence version 2. DT 11-NOV-2015, entry version 131. DE RecName: Full=Vesicular acetylcholine transporter; DE Short=VAChT; DE AltName: Full=Solute carrier family 18 member 3; GN Name=SLC18A3; Synonyms=VACHT; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE RP SPECIFICITY, AND VARIANT GLU-520. RC TISSUE=Brain; RX PubMed=8071310; RA Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., RA Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.; RT "Functional identification of a vesicular acetylcholine transporter RT and its expression from a 'cholinergic' gene locus."; RL J. Biol. Chem. 269:21929-21932(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-520. RC TISSUE=Thalamus; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15164054; DOI=10.1038/nature02462; RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P., RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., RA Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., RA Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., RA Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., RA Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., RA Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., RA Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., RA Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., RA Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., RA Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., RA Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., RA Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., RA Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., RA Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., RA Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., RA Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., RA Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., RA Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., RA Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., RA Doucette-Stamm L., Beck S., Smith D.R., Rogers J.; RT "The DNA sequence and comparative analysis of human chromosome 10."; RL Nature 429:375-381(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLU-520. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-520. RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). CC -!- FUNCTION: Involved in acetylcholine transport into synaptic CC vesicles. {ECO:0000269|PubMed:8071310}. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. CC -!- TISSUE SPECIFICITY: Peripheral and central cholinergic nervous CC systems. {ECO:0000269|PubMed:8071310}. CC -!- SIMILARITY: Belongs to the major facilitator superfamily. CC Vesicular transporter family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U10554; AAB92675.1; -; Genomic_DNA. DR EMBL; U09210; AAA20497.1; -; mRNA. DR EMBL; AK313094; BAG35918.1; -; mRNA. DR EMBL; AC073366; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CH471187; EAW93093.1; -; Genomic_DNA. DR EMBL; BC007765; AAH07765.1; -; mRNA. DR CCDS; CCDS7231.1; -. DR PIR; I38658; I38658. DR RefSeq; NP_003046.2; NM_003055.2. DR UniGene; Hs.654374; -. DR ProteinModelPortal; Q16572; -. DR STRING; 9606.ENSP00000363229; -. DR BindingDB; Q16572; -. DR ChEMBL; CHEMBL4767; -. DR GuidetoPHARMACOLOGY; 1013; -. DR TCDB; 2.A.1.2.28; the major facilitator superfamily (mfs). DR PhosphoSite; Q16572; -. DR BioMuta; SLC18A3; -. DR DMDM; 313104043; -. DR PaxDb; Q16572; -. DR PRIDE; Q16572; -. DR DNASU; 6572; -. DR Ensembl; ENST00000374115; ENSP00000363229; ENSG00000187714. DR GeneID; 6572; -. DR KEGG; hsa:6572; -. DR UCSC; uc001jhw.3; human. DR CTD; 6572; -. DR GeneCards; SLC18A3; -. DR HGNC; HGNC:10936; SLC18A3. DR HPA; CAB037102; -. DR MIM; 600336; gene. DR neXtProt; NX_Q16572; -. DR PharmGKB; PA326; -. DR eggNOG; KOG3764; Eukaryota. DR eggNOG; ENOG410XW69; LUCA. DR GeneTree; ENSGT00390000003851; -. DR HOGENOM; HOG000018545; -. DR HOVERGEN; HBG055082; -. DR InParanoid; Q16572; -. DR KO; K14636; -. DR OMA; DRMSYDV; -. DR OrthoDB; EOG7JQBN2; -. DR PhylomeDB; Q16572; -. DR TreeFam; TF313494; -. DR Reactome; R-HSA-264642; Acetylcholine Neurotransmitter Release Cycle. DR GenomeRNAi; 6572; -. DR NextBio; 25571; -. DR PRO; PR:Q16572; -. DR Proteomes; UP000005640; Chromosome 10. DR Bgee; Q16572; -. DR CleanEx; HS_SLC18A3; -. DR Genevisible; Q16572; HS. DR GO; GO:0060201; C:clathrin-sculpted acetylcholine transport vesicle membrane; TAS:Reactome. DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc. DR GO; GO:0016020; C:membrane; TAS:ProtInc. DR GO; GO:0005886; C:plasma membrane; TAS:Reactome. DR GO; GO:0043195; C:terminal bouton; IEA:Ensembl. DR GO; GO:0005277; F:acetylcholine transmembrane transporter activity; TAS:ProtInc. DR GO; GO:0015870; P:acetylcholine transport; TAS:ProtInc. DR GO; GO:0072488; P:ammonium transmembrane transport; TAS:GOC. DR GO; GO:0007269; P:neurotransmitter secretion; TAS:Reactome. DR GO; GO:0007268; P:synaptic transmission; TAS:Reactome. DR InterPro; IPR011701; MFS. DR InterPro; IPR020846; MFS_dom. DR Pfam; PF07690; MFS_1; 1. DR SUPFAM; SSF103473; SSF103473; 2. DR PROSITE; PS50850; MFS; 1. PE 2: Evidence at transcript level; KW Complete proteome; Glycoprotein; Membrane; Neurotransmitter transport; KW Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; KW Transport. FT CHAIN 1 532 Vesicular acetylcholine transporter. FT /FTId=PRO_0000127517. FT TOPO_DOM 1 33 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 34 54 Helical. {ECO:0000255}. FT TOPO_DOM 55 125 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 126 146 Helical. {ECO:0000255}. FT TOPO_DOM 147 152 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 153 173 Helical. {ECO:0000255}. FT TOPO_DOM 174 182 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 183 203 Helical. {ECO:0000255}. FT TOPO_DOM 204 213 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 214 234 Helical. {ECO:0000255}. FT TOPO_DOM 235 242 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 243 263 Helical. {ECO:0000255}. FT TOPO_DOM 264 289 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 290 310 Helical. {ECO:0000255}. FT TOPO_DOM 311 325 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 326 346 Helical. {ECO:0000255}. FT TOPO_DOM 347 356 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 357 377 Helical. {ECO:0000255}. FT TOPO_DOM 378 388 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 389 409 Helical. {ECO:0000255}. FT TOPO_DOM 410 422 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 423 443 Helical. {ECO:0000255}. FT TOPO_DOM 444 447 Lumenal, vesicle. {ECO:0000255}. FT TRANSMEM 448 468 Helical. {ECO:0000255}. FT TOPO_DOM 469 532 Cytoplasmic. {ECO:0000255}. FT CARBOHYD 89 89 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 96 96 N-linked (GlcNAc...). {ECO:0000255}. FT VARIANT 11 11 R -> Q (in dbSNP:rs8187732). FT /FTId=VAR_029152. FT VARIANT 13 13 A -> P (in dbSNP:rs8187733). FT /FTId=VAR_020034. FT VARIANT 29 29 R -> W (in dbSNP:rs8187734). FT /FTId=VAR_020035. FT VARIANT 520 520 A -> E (in dbSNP:rs8187730). FT {ECO:0000269|PubMed:14702039, FT ECO:0000269|PubMed:15489334, FT ECO:0000269|PubMed:8071310, FT ECO:0000269|Ref.4}. FT /FTId=VAR_024638. SQ SEQUENCE 532 AA; 56903 MW; 445CDF48F08ED31D CRC64; MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS PTAAWPAGSA LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVLFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR ERPVSGQDGE PRSPPGPFDA CEDDYNYYYT RS //