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Protein

Vesicular acetylcholine transporter

Gene

SLC18A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.1 Publication

GO - Molecular functioni

GO - Biological processi

  • acetylcholine transport Source: ProtInc
  • chemical synaptic transmission Source: GO_Central
  • neurotransmitter secretion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

BioCyciZFISH:G66-31718-MONOMER.
ReactomeiR-HSA-264642. Acetylcholine Neurotransmitter Release Cycle.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.

Protein family/group databases

TCDBi2.A.1.2.28. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:SLC18A3
Synonyms:VACHT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:10936. SLC18A3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 125Lumenal, vesicleSequence analysisAdd BLAST71
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 152CytoplasmicSequence analysis6
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 182Lumenal, vesicleSequence analysis9
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 213CytoplasmicSequence analysis10
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 242Lumenal, vesicleSequence analysis8
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 289CytoplasmicSequence analysisAdd BLAST26
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Topological domaini311 – 325Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 356CytoplasmicSequence analysis10
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 388Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 422CytoplasmicSequence analysisAdd BLAST13
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 447Lumenal, vesicleSequence analysis4
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 532CytoplasmicSequence analysisAdd BLAST64

GO - Cellular componenti

  • AP-1 adaptor complex Source: GO_Central
  • AP-2 adaptor complex Source: GO_Central
  • clathrin-sculpted acetylcholine transport vesicle membrane Source: Reactome
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • synaptic vesicle membrane Source: GO_Central
  • terminal bouton Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6572.
OpenTargetsiENSG00000187714.
PharmGKBiPA326.

Chemistry databases

ChEMBLiCHEMBL4767.
GuidetoPHARMACOLOGYi1013.

Polymorphism and mutation databases

BioMutaiSLC18A3.
DMDMi313104043.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275171 – 532Vesicular acetylcholine transporterAdd BLAST532

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ16572.
PRIDEiQ16572.

PTM databases

iPTMnetiQ16572.
PhosphoSitePlusiQ16572.

Expressioni

Tissue specificityi

Peripheral and central cholinergic nervous systems.1 Publication

Gene expression databases

BgeeiENSG00000187714.
CleanExiHS_SLC18A3.
GenevisibleiQ16572. HS.

Organism-specific databases

HPAiCAB037102.

Interactioni

Subunit structurei

Interacts with SEC14L1.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000363229.

Chemistry databases

BindingDBiQ16572.

Structurei

3D structure databases

ProteinModelPortaliQ16572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni471 – 532Mediates interaction with SEC14L1By similarityAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ16572.
KOiK14636.
OMAiEVWEPTL.
OrthoDBiEOG091G0534.
PhylomeDBiQ16572.
TreeFamiTF313494.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS
110 120 130 140 150
PTAAWPAGSA LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVLFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
ERPVSGQDGE PRSPPGPFDA CEDDYNYYYT RS
Length:532
Mass (Da):56,903
Last modified:November 30, 2010 - v2
Checksum:i445CDF48F08ED31D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02915211R → Q.Corresponds to variant rs8187732dbSNPEnsembl.1
Natural variantiVAR_02003413A → P.Corresponds to variant rs8187733dbSNPEnsembl.1
Natural variantiVAR_02003529R → W.Corresponds to variant rs8187734dbSNPEnsembl.1
Natural variantiVAR_024638520A → E.4 PublicationsCorresponds to variant rs8187730dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10554 Genomic DNA. Translation: AAB92675.1.
U09210 mRNA. Translation: AAA20497.1.
AK313094 mRNA. Translation: BAG35918.1.
AC073366 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93093.1.
BC007765 mRNA. Translation: AAH07765.1.
CCDSiCCDS7231.1.
PIRiI38658.
RefSeqiNP_003046.2. NM_003055.2.
UniGeneiHs.654374.

Genome annotation databases

EnsembliENST00000374115; ENSP00000363229; ENSG00000187714.
GeneIDi6572.
KEGGihsa:6572.
UCSCiuc001jhw.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10554 Genomic DNA. Translation: AAB92675.1.
U09210 mRNA. Translation: AAA20497.1.
AK313094 mRNA. Translation: BAG35918.1.
AC073366 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93093.1.
BC007765 mRNA. Translation: AAH07765.1.
CCDSiCCDS7231.1.
PIRiI38658.
RefSeqiNP_003046.2. NM_003055.2.
UniGeneiHs.654374.

3D structure databases

ProteinModelPortaliQ16572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000363229.

Chemistry databases

BindingDBiQ16572.
ChEMBLiCHEMBL4767.
GuidetoPHARMACOLOGYi1013.

Protein family/group databases

TCDBi2.A.1.2.28. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ16572.
PhosphoSitePlusiQ16572.

Polymorphism and mutation databases

BioMutaiSLC18A3.
DMDMi313104043.

Proteomic databases

PaxDbiQ16572.
PRIDEiQ16572.

Protocols and materials databases

DNASUi6572.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374115; ENSP00000363229; ENSG00000187714.
GeneIDi6572.
KEGGihsa:6572.
UCSCiuc001jhw.3. human.

Organism-specific databases

CTDi6572.
DisGeNETi6572.
GeneCardsiSLC18A3.
HGNCiHGNC:10936. SLC18A3.
HPAiCAB037102.
MIMi600336. gene.
neXtProtiNX_Q16572.
OpenTargetsiENSG00000187714.
PharmGKBiPA326.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ16572.
KOiK14636.
OMAiEVWEPTL.
OrthoDBiEOG091G0534.
PhylomeDBiQ16572.
TreeFamiTF313494.

Enzyme and pathway databases

BioCyciZFISH:G66-31718-MONOMER.
ReactomeiR-HSA-264642. Acetylcholine Neurotransmitter Release Cycle.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

GenomeRNAii6572.
PROiQ16572.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187714.
CleanExiHS_SLC18A3.
GenevisibleiQ16572. HS.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVACHT_HUMAN
AccessioniPrimary (citable) accession number: Q16572
Secondary accession number(s): B2R7S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.