Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q16572 (VACHT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular acetylcholine transporter

Short name=VAChT
Alternative name(s):
Solute carrier family 18 member 3
Gene names
Name:SLC18A3
Synonyms:VACHT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in acetylcholine transport into synaptic vesicles. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Peripheral and central cholinergic nervous systems. Ref.1

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Vesicular acetylcholine transporter
PRO_0000127517

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Potential
Topological domain55 – 12571Lumenal, vesicle Potential
Transmembrane126 – 14621Helical; Potential
Topological domain147 – 1526Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 1829Lumenal, vesicle Potential
Transmembrane183 – 20321Helical; Potential
Topological domain204 – 21310Cytoplasmic Potential
Transmembrane214 – 23421Helical; Potential
Topological domain235 – 2428Lumenal, vesicle Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 28926Cytoplasmic Potential
Transmembrane290 – 31021Helical; Potential
Topological domain311 – 32515Lumenal, vesicle Potential
Transmembrane326 – 34621Helical; Potential
Topological domain347 – 35610Cytoplasmic Potential
Transmembrane357 – 37721Helical; Potential
Topological domain378 – 38811Lumenal, vesicle Potential
Transmembrane389 – 40921Helical; Potential
Topological domain410 – 42213Cytoplasmic Potential
Transmembrane423 – 44321Helical; Potential
Topological domain444 – 4474Lumenal, vesicle Potential
Transmembrane448 – 46821Helical; Potential
Topological domain469 – 53264Cytoplasmic Potential

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential

Natural variations

Natural variant111R → Q.
Corresponds to variant rs8187732 [ dbSNP | Ensembl ].
VAR_029152
Natural variant131A → P.
Corresponds to variant rs8187733 [ dbSNP | Ensembl ].
VAR_020034
Natural variant291R → W.
Corresponds to variant rs8187734 [ dbSNP | Ensembl ].
VAR_020035
Natural variant5201A → E. Ref.1 Ref.2 Ref.4 Ref.5
Corresponds to variant rs8187730 [ dbSNP | Ensembl ].
VAR_024638

Sequences

Sequence LengthMass (Da)Tools
Q16572 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 445CDF48F08ED31D

FASTA53256,903
        10         20         30         40         50         60 
MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 

        70         80         90        100        110        120 
IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS PTAAWPAGSA LRPRYPTESE 

       130        140        150        160        170        180 
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVLFA FAEDYATLFA 

       190        200        210        220        230        240 
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 

       250        260        270        280        290        300 
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 

       310        320        330        340        350        360 
CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 

       370        380        390        400        410        420 
ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 

       430        440        450        460        470        480 
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 

       490        500        510        520        530 
ERDVLLDEPP QGLYDAVRLR ERPVSGQDGE PRSPPGPFDA CEDDYNYYYT RS 

« Hide

References

« Hide 'large scale' references
[1]"Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus."
Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.
J. Biol. Chem. 269:21929-21932(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT GLU-520.
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
Tissue: Thalamus.
[3]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-520.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10554 Genomic DNA. Translation: AAB92675.1.
U09210 mRNA. Translation: AAA20497.1.
AK313094 mRNA. Translation: BAG35918.1.
AC073366 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93093.1.
BC007765 mRNA. Translation: AAH07765.1.
CCDSCCDS7231.1.
PIRI38658.
RefSeqNP_003046.2. NM_003055.2.
UniGeneHs.654374.

3D structure databases

ProteinModelPortalQ16572.
SMRQ16572. Positions 410-442.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000363229.

Chemistry

ChEMBLCHEMBL4767.
GuidetoPHARMACOLOGY1013.

Protein family/group databases

TCDB2.A.1.2.28. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ16572.

Polymorphism databases

DMDM313104043.

Proteomic databases

PaxDbQ16572.
PRIDEQ16572.

Protocols and materials databases

DNASU6572.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374115; ENSP00000363229; ENSG00000187714.
GeneID6572.
KEGGhsa:6572.
UCSCuc001jhw.3. human.

Organism-specific databases

CTD6572.
GeneCardsGC10P050818.
HGNCHGNC:10936. SLC18A3.
HPACAB037102.
MIM600336. gene.
neXtProtNX_Q16572.
PharmGKBPA326.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255549.
HOGENOMHOG000018545.
HOVERGENHBG055082.
InParanoidQ16572.
KOK14636.
OMARPRYPTE.
OrthoDBEOG7JQBN2.
PhylomeDBQ16572.
TreeFamTF313494.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeQ16572.
CleanExHS_SLC18A3.
GenevestigatorQ16572.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi6572.
NextBio25571.
PROQ16572.
SOURCESearch...

Entry information

Entry nameVACHT_HUMAN
AccessionPrimary (citable) accession number: Q16572
Secondary accession number(s): B2R7S1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: November 30, 2010
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM