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Q16572

- VACHT_HUMAN

UniProt

Q16572 - VACHT_HUMAN

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Protein

Vesicular acetylcholine transporter

Gene

SLC18A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.1 Publication

GO - Molecular functioni

  1. acetylcholine transmembrane transporter activity Source: ProtInc

GO - Biological processi

  1. acetylcholine transport Source: ProtInc
  2. cation transmembrane transport Source: GOC
  3. neurotransmitter secretion Source: Reactome
  4. synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_15309. Acetylcholine Neurotransmitter Release Cycle.

Protein family/group databases

TCDBi2.A.1.2.28. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:SLC18A3
Synonyms:VACHT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:10936. SLC18A3.

Subcellular locationi

GO - Cellular componenti

  1. clathrin-sculpted acetylcholine transport vesicle membrane Source: Reactome
  2. integral component of plasma membrane Source: ProtInc
  3. membrane Source: ProtInc
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA326.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Vesicular acetylcholine transporterPRO_0000127517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ16572.
PRIDEiQ16572.

PTM databases

PhosphoSiteiQ16572.

Expressioni

Tissue specificityi

Peripheral and central cholinergic nervous systems.1 Publication

Gene expression databases

BgeeiQ16572.
CleanExiHS_SLC18A3.
GenevestigatoriQ16572.

Organism-specific databases

HPAiCAB037102.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000363229.

Structurei

3D structure databases

ProteinModelPortaliQ16572.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini55 – 12571Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini147 – 1526CytoplasmicSequence Analysis
Topological domaini174 – 1829Lumenal, vesicleSequence Analysis
Topological domaini204 – 21310CytoplasmicSequence Analysis
Topological domaini235 – 2428Lumenal, vesicleSequence Analysis
Topological domaini264 – 28926CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini311 – 32515Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini347 – 35610CytoplasmicSequence Analysis
Topological domaini378 – 38811Lumenal, vesicleSequence AnalysisAdd
BLAST
Topological domaini410 – 42213CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini444 – 4474Lumenal, vesicleSequence Analysis
Topological domaini469 – 53264CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei34 – 5421HelicalSequence AnalysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence AnalysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Transmembranei290 – 31021HelicalSequence AnalysisAdd
BLAST
Transmembranei326 – 34621HelicalSequence AnalysisAdd
BLAST
Transmembranei357 – 37721HelicalSequence AnalysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence AnalysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence AnalysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ16572.
KOiK14636.
OMAiRPRYPTE.
OrthoDBiEOG7JQBN2.
PhylomeDBiQ16572.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16572-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS
110 120 130 140 150
PTAAWPAGSA LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVLFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
ERPVSGQDGE PRSPPGPFDA CEDDYNYYYT RS
Length:532
Mass (Da):56,903
Last modified:November 30, 2010 - v2
Checksum:i445CDF48F08ED31D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111R → Q.
Corresponds to variant rs8187732 [ dbSNP | Ensembl ].
VAR_029152
Natural varianti13 – 131A → P.
Corresponds to variant rs8187733 [ dbSNP | Ensembl ].
VAR_020034
Natural varianti29 – 291R → W.
Corresponds to variant rs8187734 [ dbSNP | Ensembl ].
VAR_020035
Natural varianti520 – 5201A → E.4 Publications
Corresponds to variant rs8187730 [ dbSNP | Ensembl ].
VAR_024638

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10554 Genomic DNA. Translation: AAB92675.1.
U09210 mRNA. Translation: AAA20497.1.
AK313094 mRNA. Translation: BAG35918.1.
AC073366 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93093.1.
BC007765 mRNA. Translation: AAH07765.1.
CCDSiCCDS7231.1.
PIRiI38658.
RefSeqiNP_003046.2. NM_003055.2.
UniGeneiHs.654374.

Genome annotation databases

EnsembliENST00000374115; ENSP00000363229; ENSG00000187714.
GeneIDi6572.
KEGGihsa:6572.
UCSCiuc001jhw.3. human.

Polymorphism databases

DMDMi313104043.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10554 Genomic DNA. Translation: AAB92675.1 .
U09210 mRNA. Translation: AAA20497.1 .
AK313094 mRNA. Translation: BAG35918.1 .
AC073366 Genomic DNA. No translation available.
CH471187 Genomic DNA. Translation: EAW93093.1 .
BC007765 mRNA. Translation: AAH07765.1 .
CCDSi CCDS7231.1.
PIRi I38658.
RefSeqi NP_003046.2. NM_003055.2.
UniGenei Hs.654374.

3D structure databases

ProteinModelPortali Q16572.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000363229.

Chemistry

ChEMBLi CHEMBL4767.
GuidetoPHARMACOLOGYi 1013.

Protein family/group databases

TCDBi 2.A.1.2.28. the major facilitator superfamily (mfs).

PTM databases

PhosphoSitei Q16572.

Polymorphism databases

DMDMi 313104043.

Proteomic databases

PaxDbi Q16572.
PRIDEi Q16572.

Protocols and materials databases

DNASUi 6572.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000374115 ; ENSP00000363229 ; ENSG00000187714 .
GeneIDi 6572.
KEGGi hsa:6572.
UCSCi uc001jhw.3. human.

Organism-specific databases

CTDi 6572.
GeneCardsi GC10P050818.
HGNCi HGNC:10936. SLC18A3.
HPAi CAB037102.
MIMi 600336. gene.
neXtProti NX_Q16572.
PharmGKBi PA326.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG255549.
GeneTreei ENSGT00390000003851.
HOGENOMi HOG000018545.
HOVERGENi HBG055082.
InParanoidi Q16572.
KOi K14636.
OMAi RPRYPTE.
OrthoDBi EOG7JQBN2.
PhylomeDBi Q16572.
TreeFami TF313494.

Enzyme and pathway databases

Reactomei REACT_15309. Acetylcholine Neurotransmitter Release Cycle.

Miscellaneous databases

GenomeRNAii 6572.
NextBioi 25571.
PROi Q16572.
SOURCEi Search...

Gene expression databases

Bgeei Q16572.
CleanExi HS_SLC18A3.
Genevestigatori Q16572.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus."
    Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.
    J. Biol. Chem. 269:21929-21932(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT GLU-520.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
    Tissue: Thalamus.
  3. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-520.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
    Tissue: Brain.

Entry informationi

Entry nameiVACHT_HUMAN
AccessioniPrimary (citable) accession number: Q16572
Secondary accession number(s): B2R7S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: November 30, 2010
Last modified: October 29, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3