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Q16572

- VACHT_HUMAN

UniProt

Q16572 - VACHT_HUMAN

Protein

Vesicular acetylcholine transporter

Gene

SLC18A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Involved in acetylcholine transport into synaptic vesicles.1 Publication

    GO - Molecular functioni

    1. acetylcholine transmembrane transporter activity Source: ProtInc

    GO - Biological processi

    1. acetylcholine transport Source: ProtInc
    2. neurotransmitter secretion Source: Reactome
    3. synaptic transmission Source: Reactome

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_15309. Acetylcholine Neurotransmitter Release Cycle.

    Protein family/group databases

    TCDBi2.A.1.2.28. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular acetylcholine transporter
    Short name:
    VAChT
    Alternative name(s):
    Solute carrier family 18 member 3
    Gene namesi
    Name:SLC18A3
    Synonyms:VACHT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:10936. SLC18A3.

    Subcellular locationi

    GO - Cellular componenti

    1. clathrin-sculpted acetylcholine transport vesicle membrane Source: Reactome
    2. integral component of plasma membrane Source: ProtInc
    3. membrane Source: ProtInc
    4. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA326.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Vesicular acetylcholine transporterPRO_0000127517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi89 – 891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ16572.
    PRIDEiQ16572.

    PTM databases

    PhosphoSiteiQ16572.

    Expressioni

    Tissue specificityi

    Peripheral and central cholinergic nervous systems.1 Publication

    Gene expression databases

    BgeeiQ16572.
    CleanExiHS_SLC18A3.
    GenevestigatoriQ16572.

    Organism-specific databases

    HPAiCAB037102.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000363229.

    Structurei

    3D structure databases

    ProteinModelPortaliQ16572.
    SMRiQ16572. Positions 410-442.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini55 – 12571Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini147 – 1526CytoplasmicSequence Analysis
    Topological domaini174 – 1829Lumenal, vesicleSequence Analysis
    Topological domaini204 – 21310CytoplasmicSequence Analysis
    Topological domaini235 – 2428Lumenal, vesicleSequence Analysis
    Topological domaini264 – 28926CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini311 – 32515Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini347 – 35610CytoplasmicSequence Analysis
    Topological domaini378 – 38811Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini410 – 42213CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini444 – 4474Lumenal, vesicleSequence Analysis
    Topological domaini469 – 53264CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 5421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei126 – 14621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei183 – 20321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei214 – 23421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei290 – 31021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei326 – 34621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei357 – 37721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei389 – 40921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255549.
    HOGENOMiHOG000018545.
    HOVERGENiHBG055082.
    InParanoidiQ16572.
    KOiK14636.
    OMAiRPRYPTE.
    OrthoDBiEOG7JQBN2.
    PhylomeDBiQ16572.
    TreeFamiTF313494.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q16572-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESAEPAGQA RAAATKLSEA VGAALQEPRR QRRLVLVIVC VALLLDNMLY    50
    MVIVPIVPDY IAHMRGGGEG PTRTPEVWEP TLPLPTPANA SAYTANTSAS 100
    PTAAWPAGSA LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS 150
    YDVPLLIGLG VMFASTVLFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI 200
    ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA 250
    VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 300
    CNIPLAFLEP TIATWMKHTM AASEWEMGMA WLPAFVPHVL GVYLTVRLAA 350
    RYPHLQWLYG ALGLAVIGAS SCIVPACRSF APLVVSLCGL CFGIALVDTA 400
    LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ 450
    LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR 500
    ERPVSGQDGE PRSPPGPFDA CEDDYNYYYT RS 532
    Length:532
    Mass (Da):56,903
    Last modified:November 30, 2010 - v2
    Checksum:i445CDF48F08ED31D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti11 – 111R → Q.
    Corresponds to variant rs8187732 [ dbSNP | Ensembl ].
    VAR_029152
    Natural varianti13 – 131A → P.
    Corresponds to variant rs8187733 [ dbSNP | Ensembl ].
    VAR_020034
    Natural varianti29 – 291R → W.
    Corresponds to variant rs8187734 [ dbSNP | Ensembl ].
    VAR_020035
    Natural varianti520 – 5201A → E.4 Publications
    Corresponds to variant rs8187730 [ dbSNP | Ensembl ].
    VAR_024638

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10554 Genomic DNA. Translation: AAB92675.1.
    U09210 mRNA. Translation: AAA20497.1.
    AK313094 mRNA. Translation: BAG35918.1.
    AC073366 Genomic DNA. No translation available.
    CH471187 Genomic DNA. Translation: EAW93093.1.
    BC007765 mRNA. Translation: AAH07765.1.
    CCDSiCCDS7231.1.
    PIRiI38658.
    RefSeqiNP_003046.2. NM_003055.2.
    UniGeneiHs.654374.

    Genome annotation databases

    EnsembliENST00000374115; ENSP00000363229; ENSG00000187714.
    GeneIDi6572.
    KEGGihsa:6572.
    UCSCiuc001jhw.3. human.

    Polymorphism databases

    DMDMi313104043.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10554 Genomic DNA. Translation: AAB92675.1 .
    U09210 mRNA. Translation: AAA20497.1 .
    AK313094 mRNA. Translation: BAG35918.1 .
    AC073366 Genomic DNA. No translation available.
    CH471187 Genomic DNA. Translation: EAW93093.1 .
    BC007765 mRNA. Translation: AAH07765.1 .
    CCDSi CCDS7231.1.
    PIRi I38658.
    RefSeqi NP_003046.2. NM_003055.2.
    UniGenei Hs.654374.

    3D structure databases

    ProteinModelPortali Q16572.
    SMRi Q16572. Positions 410-442.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000363229.

    Chemistry

    ChEMBLi CHEMBL4767.
    GuidetoPHARMACOLOGYi 1013.

    Protein family/group databases

    TCDBi 2.A.1.2.28. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei Q16572.

    Polymorphism databases

    DMDMi 313104043.

    Proteomic databases

    PaxDbi Q16572.
    PRIDEi Q16572.

    Protocols and materials databases

    DNASUi 6572.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000374115 ; ENSP00000363229 ; ENSG00000187714 .
    GeneIDi 6572.
    KEGGi hsa:6572.
    UCSCi uc001jhw.3. human.

    Organism-specific databases

    CTDi 6572.
    GeneCardsi GC10P050818.
    HGNCi HGNC:10936. SLC18A3.
    HPAi CAB037102.
    MIMi 600336. gene.
    neXtProti NX_Q16572.
    PharmGKBi PA326.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG255549.
    HOGENOMi HOG000018545.
    HOVERGENi HBG055082.
    InParanoidi Q16572.
    KOi K14636.
    OMAi RPRYPTE.
    OrthoDBi EOG7JQBN2.
    PhylomeDBi Q16572.
    TreeFami TF313494.

    Enzyme and pathway databases

    Reactomei REACT_15309. Acetylcholine Neurotransmitter Release Cycle.

    Miscellaneous databases

    GenomeRNAii 6572.
    NextBioi 25571.
    PROi Q16572.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q16572.
    CleanExi HS_SLC18A3.
    Genevestigatori Q16572.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus."
      Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.
      J. Biol. Chem. 269:21929-21932(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT GLU-520.
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
      Tissue: Thalamus.
    3. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-520.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-520.
      Tissue: Brain.

    Entry informationi

    Entry nameiVACHT_HUMAN
    AccessioniPrimary (citable) accession number: Q16572
    Secondary accession number(s): B2R7S1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3