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Q16568

- CART_HUMAN

UniProt

Q16568 - CART_HUMAN

Protein

Cocaine- and amphetamine-regulated transcript protein

Gene

CARTPT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. activation of MAPKK activity Source: HGNC
    2. adult feeding behavior Source: HGNC
    3. cell-cell signaling Source: ProtInc
    4. cellular glucose homeostasis Source: HGNC
    5. cellular response to starvation Source: HGNC
    6. circadian regulation of gene expression Source: HGNC
    7. G-protein coupled receptor signaling pathway Source: HGNC
    8. negative regulation of appetite Source: HGNC
    9. negative regulation of bone resorption Source: HGNC
    10. negative regulation of glucagon secretion Source: Ensembl
    11. negative regulation of osteoclast differentiation Source: HGNC
    12. neuropeptide signaling pathway Source: UniProtKB-KW
    13. positive regulation of blood pressure Source: HGNC
    14. positive regulation of epinephrine secretion Source: HGNC
    15. positive regulation of transmission of nerve impulse Source: HGNC
    16. regulation of insulin secretion Source: Ensembl
    17. signal transduction Source: ProtInc
    18. somatostatin secretion Source: Ensembl
    19. synaptic transmission Source: UniProtKB-KW

    Keywords - Molecular functioni

    Neuropeptide, Neurotransmitter

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cocaine- and amphetamine-regulated transcript protein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:CARTPT
    Synonyms:CART
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:24323. CARTPT.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular space Source: HGNC
    2. secretory granule Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Keywords - Diseasei

    Obesity

    Organism-specific databases

    PharmGKBiPA162381084.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27271 PublicationAdd
    BLAST
    Chaini28 – 11689Cocaine- and amphetamine-regulated transcript proteinPRO_0000004433Add
    BLAST
    Peptidei28 – 6639CART(1-39)PRO_0000004434Add
    BLAST
    Peptidei69 – 11648CART(42-89)PRO_0000004435Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi82 ↔ 100
    Disulfide bondi88 ↔ 108
    Disulfide bondi102 ↔ 115

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    PaxDbiQ16568.
    PRIDEiQ16568.

    PTM databases

    PhosphoSiteiQ16568.

    Expressioni

    Tissue specificityi

    Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus.

    Inductioni

    By leptin.

    Gene expression databases

    BgeeiQ16568.
    CleanExiHS_CARTPT.
    GenevestigatoriQ16568.

    Organism-specific databases

    HPAiHPA046278.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CEACAM1P136883EBI-4314526,EBI-4314481
    CEACAM6P401993EBI-4314526,EBI-4314501

    Protein-protein interaction databases

    BioGridi114970. 2 interactions.
    IntActiQ16568. 2 interactions.
    STRINGi9606.ENSP00000296777.

    Structurei

    Secondary structure

    1
    116
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi84 – 863
    Beta strandi88 – 914
    Beta strandi93 – 986
    Turni110 – 1123

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HY9NMR-A76-116[»]
    ProteinModelPortaliQ16568.
    SMRiQ16568. Positions 76-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ16568.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CART family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG47455.
    HOGENOMiHOG000111306.
    HOVERGENiHBG018929.
    InParanoidiQ16568.
    OMAiSHEKELP.
    OrthoDBiEOG7JDR0T.
    PhylomeDBiQ16568.
    TreeFamiTF332948.

    Family and domain databases

    Gene3Di4.10.40.30. 1 hit.
    InterProiIPR009106. CART.
    [Graphical view]
    PANTHERiPTHR16655. PTHR16655. 1 hit.
    PfamiPF06373. CART. 1 hit.
    [Graphical view]
    SUPFAMiSSF64546. SSF64546. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q16568-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESSRVRLLP LLGAALLLML PLLGTRAQED AELQPRALDI YSAVDDASHE    50
    KELIEALQEV LKKLKSKRVP IYEKKYGQVP MCDAGEQCAV RKGARIGKLC 100
    DCPRGTSCNS FLLKCL 116
    Length:116
    Mass (Da):12,829
    Last modified:November 1, 1996 - v1
    Checksum:iFC396CA2C032AA83
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti61 – 611L → F Cosegregates with obesity phenotype in a large family. 1 Publication
    VAR_012199
    Natural varianti66 – 661S → T.1 Publication
    Corresponds to variant rs78242624 [ dbSNP | Ensembl ].
    VAR_012200
    Natural varianti113 – 1131L → M.
    Corresponds to variant rs12517689 [ dbSNP | Ensembl ].
    VAR_053022

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16826 mRNA. Translation: AAB08010.1.
    U20325 Genomic DNA. Translation: AAB08011.1.
    CR542216 mRNA. Translation: CAG47012.1.
    CH471084 Genomic DNA. Translation: EAW95694.1.
    BC029882 mRNA. Translation: AAH29882.1.
    CCDSiCCDS4011.1.
    PIRiJC4669.
    RefSeqiNP_004282.1. NM_004291.3.
    UniGeneiHs.1707.

    Genome annotation databases

    EnsembliENST00000296777; ENSP00000296777; ENSG00000164326.
    GeneIDi9607.
    KEGGihsa:9607.
    UCSCiuc003kbv.2. human.

    Polymorphism databases

    DMDMi2833274.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16826 mRNA. Translation: AAB08010.1 .
    U20325 Genomic DNA. Translation: AAB08011.1 .
    CR542216 mRNA. Translation: CAG47012.1 .
    CH471084 Genomic DNA. Translation: EAW95694.1 .
    BC029882 mRNA. Translation: AAH29882.1 .
    CCDSi CCDS4011.1.
    PIRi JC4669.
    RefSeqi NP_004282.1. NM_004291.3.
    UniGenei Hs.1707.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HY9 NMR - A 76-116 [» ]
    ProteinModelPortali Q16568.
    SMRi Q16568. Positions 76-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114970. 2 interactions.
    IntActi Q16568. 2 interactions.
    STRINGi 9606.ENSP00000296777.

    Chemistry

    DrugBanki DB00182. Amphetamine.

    PTM databases

    PhosphoSitei Q16568.

    Polymorphism databases

    DMDMi 2833274.

    Proteomic databases

    PaxDbi Q16568.
    PRIDEi Q16568.

    Protocols and materials databases

    DNASUi 9607.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296777 ; ENSP00000296777 ; ENSG00000164326 .
    GeneIDi 9607.
    KEGGi hsa:9607.
    UCSCi uc003kbv.2. human.

    Organism-specific databases

    CTDi 9607.
    GeneCardsi GC05P071051.
    HGNCi HGNC:24323. CARTPT.
    HPAi HPA046278.
    MIMi 602606. gene.
    neXtProti NX_Q16568.
    PharmGKBi PA162381084.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47455.
    HOGENOMi HOG000111306.
    HOVERGENi HBG018929.
    InParanoidi Q16568.
    OMAi SHEKELP.
    OrthoDBi EOG7JDR0T.
    PhylomeDBi Q16568.
    TreeFami TF332948.

    Miscellaneous databases

    EvolutionaryTracei Q16568.
    GenomeRNAii 9607.
    NextBioi 36043.
    PROi Q16568.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q16568.
    CleanExi HS_CARTPT.
    Genevestigatori Q16568.

    Family and domain databases

    Gene3Di 4.10.40.30. 1 hit.
    InterProi IPR009106. CART.
    [Graphical view ]
    PANTHERi PTHR16655. PTHR16655. 1 hit.
    Pfami PF06373. CART. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64546. SSF64546. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the human cDNA and genomic DNA encoding CART: a cocaine- and amphetamine-regulated transcript."
      Douglass J.O., Daoud S.
      Gene 169:241-245(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-42.
    6. Cited for: FUNCTION.
    7. "Solution structure of the satiety factor, CART, reveals new functionality of a well-known fold."
      Ludvigsen S., Thim L., Blom A.M., Wulff B.S.
      Biochemistry 40:9082-9088(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 75-116.
    8. "The CART gene and human obesity: mutational analysis and population genetics."
      Challis B.G., Yeo G.S.H., Farooqi I.S., Luan J., Aminian S., Halsall D.J., Keogh J.M., Wareham N.J., O'Rahilly S.
      Diabetes 49:872-875(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT THR-66.
    9. "Mutational screening of the CART gene in obese children: identifying a mutation (Leu34Phe) associated with reduced resting energy expenditure and cosegregating with obesity phenotype in a large family."
      del Giudice E.M., Santoro N., Cirillo G., D'Urso L., Di Toro R., Perrone L.
      Diabetes 50:2157-2160(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT PHE-61.

    Entry informationi

    Entry nameiCART_HUMAN
    AccessioniPrimary (citable) accession number: Q16568
    Secondary accession number(s): Q6FG92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3