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Q16558

- KCMB1_HUMAN

UniProt

Q16558 - KCMB1_HUMAN

Protein

Calcium-activated potassium channel subunit beta-1

Gene

KCNMB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 5 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.

    GO - Molecular functioni

    1. calcium-activated potassium channel activity Source: ProtInc
    2. potassium channel regulator activity Source: ProtInc

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. potassium ion transmembrane transport Source: GOC
    3. potassium ion transport Source: ProtInc
    4. response to calcium ion Source: Ensembl
    5. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_23767. cGMP effects.
    REACT_75896. Ca2+ activated K+ channels.

    Protein family/group databases

    TCDBi8.A.14.1.2. the ca(2+)-activated k(+) channel auxiliary subunit slowpoke- (slo) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-activated potassium channel subunit beta-1
    Alternative name(s):
    BK channel subunit beta-1
    Short name:
    BKbeta
    Short name:
    BKbeta1
    Short name:
    Hbeta1
    Calcium-activated potassium channel, subfamily M subunit beta-1
    Short name:
    Calcium-activated potassium channel subunit beta
    Charybdotoxin receptor subunit beta-1
    K(VCA)beta-1
    Maxi K channel subunit beta-1
    Slo-beta-1
    Short name:
    Slo-beta
    Gene namesi
    Name:KCNMB1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:6285. KCNMB1.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: Reactome
    2. voltage-gated potassium channel complex Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    MIMi608622. phenotype.
    PharmGKBiPA221.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 191190Calcium-activated potassium channel subunit beta-1PRO_0000187046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ16558.

    PTM databases

    PhosphoSiteiQ16558.

    Expressioni

    Tissue specificityi

    Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.

    Gene expression databases

    BgeeiQ16558.
    CleanExiHS_KCNMB1.
    GenevestigatoriQ16558.

    Organism-specific databases

    HPAiHPA020435.

    Interactioni

    Subunit structurei

    Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer.

    Protein-protein interaction databases

    BioGridi109980. 4 interactions.
    IntActiQ16558. 1 interaction.
    MINTiMINT-1470084.
    STRINGi9606.ENSP00000274629.

    Structurei

    3D structure databases

    ProteinModelPortaliQ16558.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini40 – 157118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini179 – 19113CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3921Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40171.
    HOVERGENiHBG052223.
    InParanoidiQ16558.
    KOiK04937.
    OMAiIIAMVKI.
    OrthoDBiEOG77WWDC.
    PhylomeDBiQ16558.
    TreeFamiTF328589.

    Family and domain databases

    InterProiIPR003930. K_chnl_Ca-activ_BK_bsu.
    [Graphical view]
    PANTHERiPTHR10258. PTHR10258. 1 hit.
    PfamiPF03185. CaKB. 1 hit.
    [Graphical view]
    PRINTSiPR01450. BKCHANNELB.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q16558-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVKKLVMAQK RGETRALCLG VTMVVCAVIT YYILVTTVLP LYQKSVWTQE    50
    SKCHLIETNI RDQEELKGKK VPQYPCLWVN VSAAGRWAVL YHTEDTRDQN 100
    QQCSYIPGSV DNYQTARADV EKVRAKFQEQ QVFYCFSAPR GNETSVLFQR 150
    LYGPQALLFS LFWPTFLLTG GLLIIAMVKS NQYLSILAAQ K 191
    Length:191
    Mass (Da):21,797
    Last modified:March 23, 2010 - v5
    Checksum:i547432FF194E770D
    GO
    Isoform 2 (identifier: Q16558-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         103-130: CSYIPGSVDNYQTARADVEKVRAKFQEQ → VLNWRDGDTSLYPCQVCEPVPNCPCPRG
         131-191: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:130
    Mass (Da):14,858
    Checksum:i2E612865F4130EEE
    GO

    Polymorphismi

    Genetic variation in KCNMB1 can influence the severity of diastolic hypertension.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti65 – 651E → K Has a protective effect against diastolic hypertension. 1 Publication
    Corresponds to variant rs11739136 [ dbSNP | Ensembl ].
    VAR_019325
    Natural varianti110 – 1101V → L.
    Corresponds to variant rs2301149 [ dbSNP | Ensembl ].
    VAR_047009

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei103 – 13028CSYIP…KFQEQ → VLNWRDGDTSLYPCQVCEPV PNCPCPRG in isoform 2. 1 PublicationVSP_009822Add
    BLAST
    Alternative sequencei131 – 19161Missing in isoform 2. 1 PublicationVSP_009823Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25138 mRNA. Translation: AAB02394.1.
    U38907 mRNA. Translation: AAA81327.1.
    U42600 mRNA. Translation: AAB16827.1.
    U42601 Genomic DNA. Translation: AAB16825.1.
    U42603, U42602 Genomic DNA. Translation: AAB16826.1.
    U61536 mRNA. Translation: AAB61396.1.
    U61537 Genomic DNA. Translation: AAB61397.1.
    AF026002 mRNA. Translation: AAB88805.1.
    AY044441 mRNA. Translation: AAK95827.1.
    AY515264 mRNA. Translation: AAS20193.1.
    AK313979 mRNA. Translation: BAG36693.1.
    CH471062 Genomic DNA. Translation: EAW61474.1.
    BC025707 mRNA. Translation: AAH25707.1.
    CCDSiCCDS4373.1. [Q16558-1]
    PIRiS68842.
    RefSeqiNP_004128.1. NM_004137.3. [Q16558-1]
    UniGeneiHs.484099.
    Hs.744947.

    Genome annotation databases

    EnsembliENST00000274629; ENSP00000274629; ENSG00000145936. [Q16558-1]
    ENST00000521859; ENSP00000427940; ENSG00000145936. [Q16558-2]
    GeneIDi3779.
    KEGGihsa:3779.
    UCSCiuc003maq.2. human. [Q16558-1]
    uc003mar.4. human. [Q16558-2]

    Polymorphism databases

    DMDMi292495100.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25138 mRNA. Translation: AAB02394.1 .
    U38907 mRNA. Translation: AAA81327.1 .
    U42600 mRNA. Translation: AAB16827.1 .
    U42601 Genomic DNA. Translation: AAB16825.1 .
    U42603 , U42602 Genomic DNA. Translation: AAB16826.1 .
    U61536 mRNA. Translation: AAB61396.1 .
    U61537 Genomic DNA. Translation: AAB61397.1 .
    AF026002 mRNA. Translation: AAB88805.1 .
    AY044441 mRNA. Translation: AAK95827.1 .
    AY515264 mRNA. Translation: AAS20193.1 .
    AK313979 mRNA. Translation: BAG36693.1 .
    CH471062 Genomic DNA. Translation: EAW61474.1 .
    BC025707 mRNA. Translation: AAH25707.1 .
    CCDSi CCDS4373.1. [Q16558-1 ]
    PIRi S68842.
    RefSeqi NP_004128.1. NM_004137.3. [Q16558-1 ]
    UniGenei Hs.484099.
    Hs.744947.

    3D structure databases

    ProteinModelPortali Q16558.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109980. 4 interactions.
    IntActi Q16558. 1 interaction.
    MINTi MINT-1470084.
    STRINGi 9606.ENSP00000274629.

    Chemistry

    BindingDBi Q16558.
    ChEMBLi CHEMBL3038495.

    Protein family/group databases

    TCDBi 8.A.14.1.2. the ca(2+)-activated k(+) channel auxiliary subunit slowpoke- (slo) family.

    PTM databases

    PhosphoSitei Q16558.

    Polymorphism databases

    DMDMi 292495100.

    Proteomic databases

    PRIDEi Q16558.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274629 ; ENSP00000274629 ; ENSG00000145936 . [Q16558-1 ]
    ENST00000521859 ; ENSP00000427940 ; ENSG00000145936 . [Q16558-2 ]
    GeneIDi 3779.
    KEGGi hsa:3779.
    UCSCi uc003maq.2. human. [Q16558-1 ]
    uc003mar.4. human. [Q16558-2 ]

    Organism-specific databases

    CTDi 3779.
    GeneCardsi GC05M169737.
    HGNCi HGNC:6285. KCNMB1.
    HPAi HPA020435.
    MIMi 603951. gene.
    608622. phenotype.
    neXtProti NX_Q16558.
    PharmGKBi PA221.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40171.
    HOVERGENi HBG052223.
    InParanoidi Q16558.
    KOi K04937.
    OMAi IIAMVKI.
    OrthoDBi EOG77WWDC.
    PhylomeDBi Q16558.
    TreeFami TF328589.

    Enzyme and pathway databases

    Reactomei REACT_23767. cGMP effects.
    REACT_75896. Ca2+ activated K+ channels.

    Miscellaneous databases

    GeneWikii KCNMB1.
    GenomeRNAii 3779.
    NextBioi 14829.
    PROi Q16558.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q16558.
    CleanExi HS_KCNMB1.
    Genevestigatori Q16558.

    Family and domain databases

    InterProi IPR003930. K_chnl_Ca-activ_BK_bsu.
    [Graphical view ]
    PANTHERi PTHR10258. PTHR10258. 1 hit.
    Pfami PF03185. CaKB. 1 hit.
    [Graphical view ]
    PRINTSi PR01450. BKCHANNELB.
    ProtoNeti Search...

    Publicationsi

    1. "A calcium switch for the functional coupling between alpha (hslo) and beta subunits (KV,Ca beta) of maxi K channels."
      Meera P., Wallner M., Jiang Z., Toro L.
      FEBS Lett. 382:84-88(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Uterus.
    2. "Phenotypic alteration of a human BK (hSlo) channel by hSlobeta subunit coexpression: changes in blocker sensitivity, activation/relaxation and inactivation kinetics, and protein kinase A modulation."
      Dworetzky S.I., Boissard C.G., Lum-Ragan J.T., McKay M.C., Post-Munson D.J., Trojnacki J.T., Chang C.P., Gribkoff V.K.
      J. Neurosci. 16:4543-4550(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Uterus.
    3. "Cloning, expression, and distribution of a Ca(2+)-activated K+ channel beta-subunit from human brain."
      Tseng-Crank J., Godinot N., Johansen T.E., Ahring P.K., Strobaek D., Mertz R., Foster C.D., Olesen S.P., Reinhart P.H.
      Proc. Natl. Acad. Sci. U.S.A. 93:9200-9205(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Sequence of the gene encoding the beta subunit of a human, large-conductance, calcium-activate, K+ channel."
      Folander K., Biazzo D., Swanson R.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Tissue: Aortic smooth muscle.
    5. "Identification of potassium channels in human lens epithelium."
      Rae J.L., Shepard A.R.
      (In) Civan M.M. (eds.); The eye's aqueous humor, from secretion to glaucoma, pp.69-104, Academic Press, San Diego (1998)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    6. "Calcium-activated potassium channel expression in human myometrium: effect of pregnancy."
      Mazzone J.N., Kaiser R.A., Buxton I.L.O.
      Proc. West. Pharmacol. Soc. 45:184-186(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Myometrium.
    7. "Role of the BK-beta1 subunit in human mesangial cells."
      Pluznick J.L., Sansom S.C.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    8. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Cervix.
    9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Pancreas.
    11. "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin."
      Meera P., Wallner M., Toro L.
      Proc. Natl. Acad. Sci. U.S.A. 97:5562-5567(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION.
    12. "Acute activation of Maxi-K channels (hSlo) by estradiol binding to the beta subunit."
      Valverde M.A., Rojas P., Amigo J., Cosmelli D., Orio P., Bahamonde M.I., Mann G.E., Vergara C., Latorre R.
      Science 285:1929-1931(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: BINDING TO E2.
    13. "New disguises for an old channel: MaxiK channel beta-subunits."
      Orio P., Rojas P., Ferreira G., Latorre R.
      News Physiol. Sci. 17:156-161(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    14. "Gain-of-function mutation in the KCNMB1 potassium channel subunit is associated with low prevalence of diastolic hypertension."
      Fernandez-Fernandez J.M., Tomas M., Vazquez E., Orio P., Latorre R., Senti M., Marrugat J., Valverde M.A.
      J. Clin. Invest. 113:1032-1039(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT LYS-65.

    Entry informationi

    Entry nameiKCMB1_HUMAN
    AccessioniPrimary (citable) accession number: Q16558
    Secondary accession number(s): O00707
    , O00708, P78475, Q53YR0, Q8TAX3, Q93005
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 122 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3