Reviewed, UniProtKB/Swiss-Prot Q16548 (B2LA1_HUMAN)

Names and origin

Protein names

Recommended name:
    Bcl-2-related protein A1
Alternative name(s):
    Bcl-2-like protein 5
      Short name=Bcl2-L-5
    Protein BFL-1
    Hemopoietic-specific early response protein
    Protein GRS

Gene names

Name:	BCL2A1
Synonyms:	BCL2L5, BFL1, GRS, HBPA1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection By similarity.
Subunit structure	Interacts directly with BAK1, BID, BMF and BBC3 By similarity. Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Ref.8
Subcellular location	Cytoplasm.
Tissue specificity	Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
Induction	By phorbol ester and inflammatory cytokines, such as TNF-alpha, or IL-1-beta, but not by growth factors.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.2 Traceable author statement. Source: ProtInc apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BBC3	Q9BXH1	1	EBI-1003550,EBI-519884
BCL2L11	O43521	1	EBI-1003550,EBI-526406
BID	P55957	1	EBI-1003550,EBI-519672
NR4A1	P22736	1	EBI-1003550,EBI-721550

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 175

175

Bcl-2-related protein A1

PRO_0000143094

Regions

Motif

77 – 97

21

BH1

Motif

132 – 147

16

BH2

Compositional bias

24 – 33

10

Ala/Pro-rich

Natural variations

Natural variant

19

1

C → Y: dbSNP rs1138357. Ref.5

VAR_020342

Natural variant

39

1

N → K: dbSNP rs1138358. Ref.5

VAR_020343

Natural variant

82

1

G → D: dbSNP rs3826007. Ref.5

VAR_020344

Natural variant

117

1

E → D: dbSNP rs34080999.

VAR_044059

Experimental info

Sequence conflict

72

1

N → T in CAA70566. Ref.3

Sequence conflict

107

1

Q → H in CAA70566. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

175

Helix Strand Turn

Details...

Helix

1 – 20

20

Helix

32 – 51

20

Helix

53 – 56

4

Helix

64 – 79

16

Helix

86 – 106

21

Helix

113 – 115

3

Helix

116 – 136

21

Helix

139 – 142

4

Helix

144 – 148

5

Sequences

	Sequence		Length	Mass (Da)
	Q16548-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 329D98AF2BE07A0D	FASTA	175	20,132
	10 20 30 40 50 60 MTDCEFGYIY RLAQDYLQCV LQIPQPGSGP SKTSRVLQNV AFSVQKEVEK NLKSCLDNVN 70 80 90 100 110 120 VVSVDTARTL FNQVMEKEFE DGIINWGRIV TIFAFEGILI KKLLRQQIAP DVDTYKEISY 130 140 150 160 170 FVAEFIMNNT GEWIRQNGGW ENGFVKKFEP KSGWMTFLEV TGKICEMLSL LKQYC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow." Choi S.S., Park I.-C., Yun J.W., Sung Y.C., Hong S.-I., Shin H.-S. Oncogene 11:1693-1698(1995) [PubMed: 7478596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning of human Bcl-2 homologue: inflammatory cytokines induce human A1 in cultured endothelial cells." Karsan A., Yee E., Kaushansky K., Harlan J.M. Blood 87:3089-3096(1996) [PubMed: 8605321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical vein.
[3]	"GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes." Kenny J.J., Knobloch T.J., Augustus M., Carter K.C., Rosen C.A., Lang J.C. Oncogene 14:997-1001(1997) [PubMed: 9050999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TYR-19; LYS-39 AND ASP-82.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[8]	"Characterization of Bax-sigma, a cell death-inducing isoform of Bax." Schmitt E., Paquet C., Beauchemin M., Dever-Bertrand J., Bertrand R. Biochem. Biophys. Res. Commun. 270:868-879(2000) [PubMed: 10772918] [Abstract] Cited for: INTERACTION WITH BAX.
[9]	"Human BCL-2A1 in complex with BIM." Structural genomics consortium (SGC) Submitted (FEB-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 149-608 IN COMPLEX WITH BCL2L11.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U27467 mRNA. Translation: AAC50288.1.
U29680 mRNA. Translation: AAC50438.1.
Y09397 mRNA. Translation: CAA70566.1.
BT007103 mRNA. Translation: AAP35767.1.
CR541937 mRNA. Translation: CAG46735.1.
CR541962 mRNA. Translation: CAG46760.1.
CH471136 Genomic DNA. Translation: EAW99129.1.
BC016281 mRNA. Translation: AAH16281.1.

IPI

IPI00002881.

PIR

I39055.

RefSeq

NP_004040.1.

UniGene

Hs.227817

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VM6	X-ray	2.20	A	1-149	[»]
3I1H	X-ray	2.20	A	1-151	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q16548. 4 interactions.

STRING

Q16548.

Proteomic databases

PRIDE

Q16548.

Genome annotation databases

Ensembl

ENST00000267953; ENSP00000267953; ENSG00000140379; Homo sapiens. [Genome view]

GeneID

597.

UCSC

uc002bfc.2. human.

Organism-specific databases

CTD

597.

GeneCards

GC15M078040.

H-InvDB

HIX0012488.

HGNC

HGNC:991. BCL2A1.

HPA

CAB010382.

MIM

601056. gene.

PharmGKB

PA25303.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG06809.

HOGENOM

HBG127246.

HOVERGEN

Q16548.

InParanoid

Q16548.

OrthoDB

EOG9WDGX1.

PhylomeDB

Q16548.

Enzyme and pathway databases

Pathway_Interaction_DB

bcr_5pathway. BCR signaling pathway.

Gene expression databases

ArrayExpress

Q16548.

Bgee

Q16548.

CleanEx

HS_BCL2A1.

Genevestigator

Q16548.

GermOnline

ENSG00000140379. Homo sapiens.

Family and domain databases

InterPro

IPR013282. Apop_reg_Bcl2Rel.
IPR002475. BCL2_apoptsis.
IPR000712. Bcl2_BH.
IPR020717. Bcl2_BH1_motif_CS.
IPR020726. Bcl2_BH2_motif_CS.
[Graphical view]

Pfam

PF00452. Bcl-2. 1 hit.
[Graphical view]

PRINTS

PR01862. BCL2FAMILY.
PR01867. BCL2RLATEDA1.

SMART

SM00337. BCL. 1 hit.
[Graphical view]

PROSITE

PS50062. BCL2_FAMILY. 1 hit.
PS01080. BH1. 1 hit.
PS01258. BH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

2429.

SOURCE

Search...

Entry information

Entry name

B2LA1_HUMAN

Accession

Primary (citable) accession number: Q16548
Secondary accession number(s): Q6FGZ4, Q6FH19, Q99524

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.