Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q16537 (2A5E_HUMAN)

Last modified February 9, 2010. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
Alternative name(s):
    PP2A B subunit isoform B'-epsilon
    PP2A B subunit isoform B56-epsilon
    PP2A B subunit isoform PR61-epsilon
    PP2A B subunit isoform R5-epsilon

Gene names

Name:

PPP2R5E

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. Ref.4
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated on serine residues. Ref.6
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B56 family.

Hide | Top

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: HPA intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA protein phosphatase type 2A complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 2A regulator activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHEK2	O96017	3	EBI-968374,EBI-1180783
PPP2R1B	P30154	1	EBI-968374,EBI-357094

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 467

467

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

PRO_0000071454

Amino acid modifications

Modified residue

Phosphothreonine Ref.6

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q16537-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DD9CE11433F499CF
Show »

FASTA

467

54,699

        10         20         30         40         50         60 
MSSAPTTPPS VDKVDGFSRK SVRKARQKRS QSSSQFRSQG KPIELTPLPL LKDVPSSEQP 

        70         80         90        100        110        120 
ELFLKKLQQC CVIFDFMDTL SDLKMKEYKR STLNELVDYI TISRGCLTEQ TYPEVVRMVS 

       130        140        150        160        170        180 
CNIFRTLPPS DSNEFDPEED EPTLEASWPH LQLVYEFFIR FLESQEFQPS IAKKYIDQKF 

       190        200        210        220        230        240 
VLQLLELFDS EDPRERDYLK TVLHRIYGKF LGLRAFIRKQ INNIFLRFVY ETEHFNGVAE 

       250        260        270        280        290        300 
LLEILGSIIN GFALPLKAEH KQFLVKVLIP LHTVRSLSLF HAQLAYCIVQ FLEKDPSLTE 

       310        320        330        340        350        360 
PVIRGLMKFW PKTCSQKEVM FLGELEEILD VIEPSQFVKI QEPLFKQIAK CVSSPHFQVA 

       370        380        390        400        410        420 
ERALYYWNNE YIMSLIEENS NVILPIMFSS LYRISKEHWN PAIVALVYNV LKAFMEMNST 

       430        440        450        460 
MFDELTATYK SDRQREKKKE KEREELWKKL EDLELKRGLR RDGIIPT

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The variable subunit associated with protein phosphatase 2A0 defines a novel multimember family of regulatory subunits." Zolnierowicz S., van Hoof C., Andjelkovic N., Cron P., Stevens I., Merlevede W., Goris J., Hemmings B.A. Biochem. J. 317:187-194(1996) [PubMed: 8694763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 449-455. Tissue: Fetal retina.
[2]	"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm." McCright B., Rivers A.M., Audlin S., Virshup D.M. J. Biol. Chem. 271:22081-22089(1996) [PubMed: 8703017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Shugoshin collaborates with protein phosphatase 2A to protect cohesin." Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y. Nature 441:46-52(2006) [PubMed: 16541025] [Abstract] Cited for: INTERACTION WITH SGOL1.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-7, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69029 mRNA. Translation: CAA93153.1. L76703 mRNA. Translation: AAB69752.1. BC093766 mRNA. Translation: AAH93766.1. BC101479 mRNA. Translation: AAI01480.1. BC143231 mRNA. Translation: AAI43232.1.
IPI	IPI00002853.
RefSeq	NP_006237.1.
UniGene	Hs.334868
3D structure databases
SMR	Q16537. Positions 51-423.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16537. 7 interactions.
STRING	Q16537.
PTM databases
PhosphoSite	Q16537.
Proteomic databases
PRIDE	Q16537.
Genome annotation databases
Ensembl	ENST00000337537; ENSP00000337641; ENSG00000154001; Homo sapiens. [Genome view] ENST00000422769; ENSP00000404632; ENSG00000154001; Homo sapiens. [Genome view]
GeneID	5529.
KEGG	hsa:5529.
UCSC	uc001xgd.1. human.
Organism-specific databases
CTD	5529.
GeneCards	GC14M062911.
H-InvDB	HIX0037720.
HGNC	HGNC:9313. PPP2R5E.
HPA	HPA006034.
MIM	601647. gene.
PharmGKB	PA33677.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17395.
HOVERGEN	Q16537.
InParanoid	Q16537.
OrthoDB	EOG970X2R.
PhylomeDB	Q16537.
Gene expression databases
ArrayExpress	Q16537.
Bgee	Q16537.
CleanEx	HS_PPP2R5E.
Genevestigator	Q16537.
GermOnline	ENSG00000154001. Homo sapiens.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR002554. PP2A_B56. [Graphical view]
PANTHER	PTHR10257. B56. 1 hit.
Pfam	PF01603. B56. 1 hit. [Graphical view]
PIRSF	PIRSF028043. PP2A_B56. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	21418.
SOURCE	Search...

Hide | Top

Entry information

Entry name

2A5E_HUMAN

Accession

Primary (citable) accession number: Q16537
Secondary accession number(s): A4FU37, B7ZKK8, Q52LW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Hide | Top

Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents