Q16537 (2A5E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

Alternative name(s):
PP2A B subunit isoform B'-epsilon
PP2A B subunit isoform B56-epsilon
PP2A B subunit isoform PR61-epsilon
PP2A B subunit isoform R5-epsilon

Gene names

Name:

PPP2R5E

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	Found in a complex with at least ARL2, PPP2CB; PPP2R1A, PPP2R2A, PPP2R5E and TBCD By similarity. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. Ref.4
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated on serine residues.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B56 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA protein phosphatase type 2A complex Inferred from electronic annotation. Source: InterPro
Molecular_function	protein phosphatase type 2A regulator activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHEK2	O96017	3	EBI-968374,EBI-1180783
PPP2R1A	P30153	3	EBI-968374,EBI-302388
PPP2R1B	P30154	3	EBI-968374,EBI-357094

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform		PRO_0000071454
Amino acid modifications
	Modified residue	30	1	Phosphoserine Ref.8
	Modified residue	32	1	Phosphoserine Ref.8
	Modified residue	34	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q16537 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DD9CE11433F499CF	FASTA	467	54,699
	10 20 30 40 50 60 MSSAPTTPPS VDKVDGFSRK SVRKARQKRS QSSSQFRSQG KPIELTPLPL LKDVPSSEQP 70 80 90 100 110 120 ELFLKKLQQC CVIFDFMDTL SDLKMKEYKR STLNELVDYI TISRGCLTEQ TYPEVVRMVS 130 140 150 160 170 180 CNIFRTLPPS DSNEFDPEED EPTLEASWPH LQLVYEFFIR FLESQEFQPS IAKKYIDQKF 190 200 210 220 230 240 VLQLLELFDS EDPRERDYLK TVLHRIYGKF LGLRAFIRKQ INNIFLRFVY ETEHFNGVAE 250 260 270 280 290 300 LLEILGSIIN GFALPLKAEH KQFLVKVLIP LHTVRSLSLF HAQLAYCIVQ FLEKDPSLTE 310 320 330 340 350 360 PVIRGLMKFW PKTCSQKEVM FLGELEEILD VIEPSQFVKI QEPLFKQIAK CVSSPHFQVA 370 380 390 400 410 420 ERALYYWNNE YIMSLIEENS NVILPIMFSS LYRISKEHWN PAIVALVYNV LKAFMEMNST 430 440 450 460 MFDELTATYK SDRQREKKKE KEREELWKKL EDLELKRGLR RDGIIPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The variable subunit associated with protein phosphatase 2A0 defines a novel multimember family of regulatory subunits." Zolnierowicz S., van Hoof C., Andjelkovic N., Cron P., Stevens I., Merlevede W., Goris J., Hemmings B.A. Biochem. J. 317:187-194(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 449-455. Tissue: Fetal retina.
[2]	"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm." McCright B., Rivers A.M., Audlin S., Virshup D.M. J. Biol. Chem. 271:22081-22089(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Shugoshin collaborates with protein phosphatase 2A to protect cohesin." Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y. Nature 441:46-52(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SGOL1.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30; SER-32 AND SER-34, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z69029 mRNA. Translation: CAA93153.1. L76703 mRNA. Translation: AAB69752.1. BC093766 mRNA. Translation: AAH93766.1. BC101479 mRNA. Translation: AAI01480.1. BC143231 mRNA. Translation: AAI43232.1.
IPI	IPI00002853.
RefSeq	NP_006237.1. NM_006246.2.
UniGene	Hs.334868.
3D structure databases
ProteinModelPortal	Q16537.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16537. 25 interactions.
MINT	MINT-1198703.
STRING	9606.ENSP00000337641.
PTM databases
PhosphoSite	Q16537.
Polymorphism databases
DMDM	7387498.
Proteomic databases
PaxDb	Q16537.
PRIDE	Q16537.
Protocols and materials databases
DNASU	5529.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337537; ENSP00000337641; ENSG00000154001.
GeneID	5529.
KEGG	hsa:5529.
UCSC	uc001xgd.1. human.
Organism-specific databases
CTD	5529.
GeneCards	GC14M063838.
HGNC	HGNC:9313. PPP2R5E.
HPA	HPA006034.
MIM	601647. gene.
neXtProt	NX_Q16537.
PharmGKB	PA33677.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264925.
HOGENOM	HOG000067326.
HOVERGEN	HBG000009.
InParanoid	Q16537.
KO	K11584.
OMA	VSRGYLT.
OrthoDB	EOG483D4M.
PhylomeDB	Q16537.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases. REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q16537.
Bgee	Q16537.
CleanEx	HS_PPP2R5E.
Genevestigator	Q16537.
GermOnline	ENSG00000154001. Homo sapiens.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR002554. PP2A_B56. [Graphical view]
PANTHER	PTHR10257. PTHR10257. 1 hit.
Pfam	PF01603. B56. 1 hit. [Graphical view]
PIRSF	PIRSF028043. PP2A_B56. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PPP2R5E. human.
GenomeRNAi	5529.
NextBio	21418.
SOURCE	Search...

Entry information

Entry name

2A5E_HUMAN

Accession

Primary (citable) accession number: Q16537
Secondary accession number(s): A4FU37, B7ZKK8, Q52LW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.