Q16534 (HLF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hepatic leukemia factor

Gene names

Name:

HLF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subunit structure	Binds DNA specifically as homodimer or heterodimer with other PAR factors.
Subcellular location	Nucleus Probable.
Tissue specificity	Highly expressed in liver; lower levels in lung and kidney.
Induction	Accumulates according to a robust circadian rhythm By similarity.
Involvement in disease	A chromosomal aberration involving HLF is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(17;19)(q22;p13.3) with TCF3.
Sequence similarities	Belongs to the bZIP family. PAR subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence AAA58445.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Biological rhythms Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Chromosomal rearrangement
Disease	Proto-oncogene
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Traceable author statement Ref.2. Source: ProtInc rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Traceable author statement PubMed 8639829. Source: ProtInc
Cellular_component	nucleus Traceable author statement Ref.2. Source: ProtInc
Molecular_function	double-stranded DNA binding Traceable author statement Ref.2. Source: ProtInc sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Hepatic leukemia factor		PRO_0000076510
Regions
	Domain	225 – 288	64	bZIP
	Region	227 – 247	21	Basic motif By similarity
	Region	248 – 255	8	Leucine-zipper By similarity
	Compositional bias	158 – 209	52	Pro-rich (proline/acidic region (PAR))
Natural variations
	Natural variant	253	1	I → F in fusion protein; decreases DNA-binding activity.		VAR_008515

Sequences

	Sequence		Length	Mass (Da)
	Q16534 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 80572A727BBB5635	FASTA	295	33,199
	10 20 30 40 50 60 MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP 70 80 90 100 110 120 QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA 130 140 150 160 170 180 PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA 190 200 210 220 230 240 DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK 250 260 270 280 290 RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hlf, a novel hepatic bZIP protein, shows altered DNA-binding properties following fusion to E2A in t(17;19) acute lymphoblastic leukemia." Hunger S.P., Ohyashiki K., Toyama K., Cleary M.L. Genes Dev. 6:1608-1620(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH TCF3. Tissue: Hepatoma.
[2]	"Fusion of the leucine zipper gene HLF to the E2A gene in human acute B-lineage leukemia." Inaba T., Roberts W.M., Shapiro L.H., Jolly K.W., Raimondi S.C., Smith S.D., Look A.T. Science 257:531-534(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH TCF3. Tissue: Hepatoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95585 mRNA. Translation: AAA52675.1. M95586 mRNA. Translation: AAA58445.1. Different initiation. X68985 mRNA. Translation: CAA48777.1. BC036093 mRNA. Translation: AAH36093.1.
IPI	IPI00002850.
PIR	A44064.
RefSeq	NP_002117.1. NM_002126.4.
UniGene	Hs.196952.
3D structure databases
ProteinModelPortal	Q16534.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16534. 25 interactions.
STRING	9606.ENSP00000226067.
PTM databases
PhosphoSite	Q16534.
Polymorphism databases
DMDM	6919907.
Proteomic databases
PaxDb	Q16534.
PRIDE	Q16534.
Protocols and materials databases
DNASU	3131.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000226067; ENSP00000226067; ENSG00000108924.
GeneID	3131.
KEGG	hsa:3131.
UCSC	uc002iug.1. human.
Organism-specific databases
CTD	3131.
GeneCards	GC17P053342.
HGNC	HGNC:4977. HLF.
MIM	142385. gene.
neXtProt	NX_Q16534.
Orphanet	99860. Precursor B-cell acute lymphoblastic leukemia.
PharmGKB	PA29311.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG329624.
HOGENOM	HOG000234383.
HOVERGEN	HBG004983.
InParanoid	Q16534.
KO	K09057.
OMA	PTYGVLK.
OrthoDB	EOG4QNMWZ.
PhylomeDB	Q16534.
Gene expression databases
ArrayExpress	Q16534.
Bgee	Q16534.
CleanEx	HS_HLF.
Genevestigator	Q16534.
GermOnline	ENSG00000108924. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3131.
NextBio	12421.
SOURCE	Search...

Entry information

Entry name

HLF_HUMAN

Accession

Primary (citable) accession number: Q16534

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.