Q16520 (BATF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Basic leucine zipper transcriptional factor ATF-like

Alternative name(s):
B-cell-activating transcription factor
Short name=B-ATF
SF-HT-activated gene 2 protein
Short name=SFA-2

Gene names

Name:

BATF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate to CD8+ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8+ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs By similarity.
Subunit structure	Heterodimer; mainly heterodimerizes with JUNB. The BATF-JUNB heterodimer interacts with IRF4 and IRF8. Interacts (via bZIP domain) with IRF4 and IRF8; the interaction is direct By similarity. Also forms heterodimers with JUN and JUND. Also interacts with IFI35. Ref.1 Ref.6
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells By similarity.
Tissue specificity	Expressed at highest levels in lung, and at lower levels in placenta, liver, kidney, spleen, and peripheral blood. Detected in SW480 colorectal cancer cell line and several hematopoietic tumor cell lines, including Raji Burkitt's lymphoma. Strongly expressed in mature B- and T-lymphocytes. Also expressed in moderate levels in lymph node and appendix and at low levels in thymus and bone marrow (Ref.7). Ref.1 Ref.2 Ref.7
Induction	Up-regulated by PDCD1 following infection by HIV-1 virus, leading to inhibit T-cell functions and exhaust T-cells. Up-regulated by Epstein-Barr virus (EBV) protein EBNA2 following infection by EBV. Ref.8 Ref.9
Post-translational modification	Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription By similarity. Phosphorylated By similarity.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, signal transduction by p53 class mediator Inferred from sequence or structural similarity. Source: UniProtKB T-helper 17 cell lineage commitment Inferred from sequence or structural similarity. Source: UniProtKB T-helper 2 cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB cytokine production Inferred from sequence or structural similarity. Source: UniProtKB defense response to protozoan Inferred from sequence or structural similarity. Source: UniProtKB hematopoietic stem cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB isotype switching Inferred from sequence or structural similarity. Source: UniProtKB lymphoid progenitor cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB myeloid dendritic cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 125	125	Basic leucine zipper transcriptional factor ATF-like		PRO_0000076595
Regions
	Domain	26 – 89	64	bZIP
	Region	28 – 50	23	Basic motif By similarity
	Region	54 – 75	22	Leucine-zipper By similarity
Amino acid modifications
	Modified residue	43	1	Phosphoserine By similarity
	Modified residue	48	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q16520 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7FD633C6F1963DF4	FASTA	125	14,120
	10 20 30 40 50 60 MPHSSDSSDS SFSRSPPPGK QDSSDDVRRV QRREKNRIAA QKSRQRQTQK ADTLHLESED 70 80 90 100 110 120 LEKQNAALRK EIKQLTEELK YFTSVLNSHE PLCSVLAAST PSPPEVVYSA HAFHQPHVSS PRFQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family." Dorsey M.J., Tae H.-J., Sollenberger K.G., Mascarenhas N.T., Johansen L.M., Taparowsky E.J. Oncogene 11:2255-2265(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH JUN PROTEINS, TISSUE SPECIFICITY.
[2]	"SFA-2, a novel bZIP transcription factor induced by human T-cell leukemia virus type I, is highly expressed in mature lymphocytes." Hasegawa H., Utsunomiya Y., Kishimoto K., Tange Y., Yasukawa M., Fujita S. Biochem. Biophys. Res. Commun. 222:164-170(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"Genomic organization of human B-ATF, a target for regulation by EBV and HTLV-1." Meyer N.P., Johansen L.M., Tae H.-J., Budde P.P., Williams K.L., Taparowsky E.J. Mamm. Genome 9:849-852(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[6]	"IFP 35 forms complexes with B-ATF, a member of the AP1 family of transcription factors." Wang X., Johansen L.M., Tae H.-J., Taparowsky E.J. Biochem. Biophys. Res. Commun. 229:316-322(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IFI35.
[7]	"B-ATF functions as a negative regulator of AP-1 mediated transcription and blocks cellular transformation by Ras and Fos." Echlin D.R., Tae H.-J., Mitin N., Taparowsky E.J. Oncogene 19:1752-1763(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"EBNA2 and activated Notch induce expression of BATF." Johansen L.M., Deppmann C.D., Erickson K.D., Coffin W.F. III, Thornton T.M., Humphrey S.E., Martin J.M., Taparowsky E.J. J. Virol. 77:6029-6040(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"Transcriptional analysis of HIV-specific CD8+ T cells shows that PD-1 inhibits T cell function by upregulating BATF." Quigley M., Pereyra F., Nilsson B., Porichis F., Fonseca C., Eichbaum Q., Julg B., Jesneck J.L., Brosnahan K., Imam S., Russell K., Toth I., Piechocka-Trocha A., Dolfi D., Angelosanto J., Crawford A., Shin H., Kwon D.S. , Zupkosky J., Francisco L., Freeman G.J., Wherry E.J., Kaufmann D.E., Walker B.D., Ebert B., Haining W.N. Nat. Med. 16:1147-1151(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15460 mRNA. Translation: AAC50314.1. D42106 mRNA. Translation: BAA07686.1. AF016898 Genomic DNA. Translation: AAC78243.1. AC007182 Genomic DNA. Translation: AAD51372.1. BC032294 mRNA. Translation: AAH32294.1.
IPI	IPI00007422.
PIR	JC4799.
RefSeq	NP_006390.1. NM_006399.3.
UniGene	Hs.509964.
3D structure databases
ProteinModelPortal	Q16520.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16520. 3 interactions.
MINT	MINT-1440747.
STRING	9606.ENSP00000286639.
PTM databases
PhosphoSite	Q16520.
Polymorphism databases
DMDM	32171340.
Proteomic databases
PaxDb	Q16520.
PRIDE	Q16520.
Protocols and materials databases
DNASU	10538.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000286639; ENSP00000286639; ENSG00000156127.
GeneID	10538.
KEGG	hsa:10538.
UCSC	uc001xrr.3. human.
Organism-specific databases
CTD	10538.
GeneCards	GC14P075988.
HGNC	HGNC:958. BATF.
MIM	612476. gene.
neXtProt	NX_Q16520.
PharmGKB	PA25268.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG257556.
HOGENOM	HOG000236325.
HOVERGEN	HBG039173.
InParanoid	Q16520.
KO	K09034.
OMA	THAFHQP.
OrthoDB	EOG48GW4S.
PhylomeDB	Q16520.
Gene expression databases
ArrayExpress	Q16520.
Bgee	Q16520.
CleanEx	HS_BATF.
Genevestigator	Q16520.
GermOnline	ENSG00000156127. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. IPR000837. Leuzip_Fos. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
PRINTS	PR00042. LEUZIPPRFOS.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10538.
NextBio	39981.
SOURCE	Search...

Entry information

Entry name

BATF_HUMAN

Accession

Primary (citable) accession number: Q16520

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.