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Protein

Transcription initiation factor TFIID subunit 12

Gene

TAF12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription.

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • RNA polymerase II activating transcription factor binding Source: GO_Central
  • TBP-class protein binding Source: GO_Central
  • transcription coactivator activity Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120656-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-3214847. HATs acetylate histones.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ16514.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 12
Alternative name(s):
Transcription initiation factor TFIID 20/15 kDa subunits
Short name:
TAFII-20/TAFII-15
Short name:
TAFII20/TAFII15
Gene namesi
Name:TAF12
Synonyms:TAF15, TAF2J, TAFII20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11545. TAF12.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Reactome
  • PCAF complex Source: UniProtKB
  • SAGA complex Source: GO_Central
  • STAGA complex Source: UniProtKB
  • transcription factor TFIID complex Source: MGI
  • transcription factor TFTC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6883.
OpenTargetsiENSG00000120656.
PharmGKBiPA36320.

Polymorphism and mutation databases

BioMutaiTAF12.
DMDMi3024708.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000335801 – 161Transcription initiation factor TFIID subunit 12Add BLAST161

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphothreonineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei59PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ16514.
MaxQBiQ16514.
PaxDbiQ16514.
PeptideAtlasiQ16514.
PRIDEiQ16514.

PTM databases

iPTMnetiQ16514.
PhosphoSitePlusiQ16514.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000120656.
CleanExiHS_TAF12.
HS_TAF15.
GenevisibleiQ16514. HS.

Organism-specific databases

HPAiHPA008519.

Interactioni

Subunit structurei

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP; additional interactions between TAFII20 and TAFII28 or TAFII30 were detected. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, SUPT3H, TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, STAF65-gamma/KIAA0764, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Interacts (isoform TAFII15 and isoform TAFII20) with ATF7 (via the transactivation domain); the interaction is prevented by sumoylation of ATF7 and promotes (isoform TAFII20 only) the transactivation of ATF7.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKAR2BQ6DHZ23EBI-1034238,EBI-10185196
TAF1CQ155722EBI-1034238,EBI-2510659
TBPP202262EBI-1034238,EBI-355371

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112746. 51 interactors.
DIPiDIP-496N.
IntActiQ16514. 23 interactors.
MINTiMINT-3032603.
STRINGi9606.ENSP00000263974.

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi60 – 70Combined sources11
Helixi78 – 105Combined sources28
Helixi113 – 123Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3OX-ray2.30B/D57-128[»]
ProteinModelPortaliQ16514.
SMRiQ16514.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16514.

Family & Domainsi

Sequence similaritiesi

Belongs to the TAF12 family.Curated
Contains 1 histone-fold domain.Curated

Phylogenomic databases

eggNOGiKOG1142. Eukaryota.
COG5624. LUCA.
GeneTreeiENSGT00390000002144.
HOGENOMiHOG000035092.
HOVERGENiHBG055174.
InParanoidiQ16514.
KOiK03126.
OMAiHSSMANN.
OrthoDBiEOG091G0Q1X.
PhylomeDBiQ16514.
TreeFamiTF323652.

Family and domain databases

CDDicd07981. TAF12. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003228. TFIID_TAF12.
[Graphical view]
PfamiPF03847. TFIID_20kDa. 1 hit.
[Graphical view]
ProDomiPD012998. PD012998. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform TAFII20 (identifier: Q16514-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
60 70 80 90 100
SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIADD FIESVVTAAC
110 120 130 140 150
QLARHRKSST LEVKDVQLHL ERQWNMWIPG FGSEEIRPYK KACTTEAHKQ
160
RMALIRKTTK K
Length:161
Mass (Da):17,924
Last modified:November 1, 1996 - v1
Checksum:i054B017CD7BF99CE
GO
Isoform TAFII15 (identifier: Q16514-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:131
Mass (Da):14,825
Checksum:i655B769918311CE1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188881 – 30Missing in isoform TAFII15. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57693 mRNA. Translation: AAC50600.1.
U57693 mRNA. Translation: AAC50601.1.
D50544 mRNA. Translation: BAA09112.1.
X84002 mRNA. Translation: CAA58826.1.
BT007031 mRNA. Translation: AAP35678.1.
AY206865 Genomic DNA. Translation: AAO13491.1.
AL513497 Genomic DNA. Translation: CAI22291.1.
CH471059 Genomic DNA. Translation: EAX07682.1.
CH471059 Genomic DNA. Translation: EAX07683.1.
BC011986 mRNA. Translation: AAH11986.1.
CCDSiCCDS326.1. [Q16514-1]
PIRiJC4676.
RefSeqiNP_001128690.1. NM_001135218.1. [Q16514-1]
NP_005635.1. NM_005644.3. [Q16514-1]
UniGeneiHs.530251.

Genome annotation databases

EnsembliENST00000263974; ENSP00000263974; ENSG00000120656. [Q16514-1]
ENST00000373824; ENSP00000362930; ENSG00000120656. [Q16514-1]
GeneIDi6883.
KEGGihsa:6883.
UCSCiuc001bqx.5. human. [Q16514-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57693 mRNA. Translation: AAC50600.1.
U57693 mRNA. Translation: AAC50601.1.
D50544 mRNA. Translation: BAA09112.1.
X84002 mRNA. Translation: CAA58826.1.
BT007031 mRNA. Translation: AAP35678.1.
AY206865 Genomic DNA. Translation: AAO13491.1.
AL513497 Genomic DNA. Translation: CAI22291.1.
CH471059 Genomic DNA. Translation: EAX07682.1.
CH471059 Genomic DNA. Translation: EAX07683.1.
BC011986 mRNA. Translation: AAH11986.1.
CCDSiCCDS326.1. [Q16514-1]
PIRiJC4676.
RefSeqiNP_001128690.1. NM_001135218.1. [Q16514-1]
NP_005635.1. NM_005644.3. [Q16514-1]
UniGeneiHs.530251.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3OX-ray2.30B/D57-128[»]
ProteinModelPortaliQ16514.
SMRiQ16514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112746. 51 interactors.
DIPiDIP-496N.
IntActiQ16514. 23 interactors.
MINTiMINT-3032603.
STRINGi9606.ENSP00000263974.

PTM databases

iPTMnetiQ16514.
PhosphoSitePlusiQ16514.

Polymorphism and mutation databases

BioMutaiTAF12.
DMDMi3024708.

Proteomic databases

EPDiQ16514.
MaxQBiQ16514.
PaxDbiQ16514.
PeptideAtlasiQ16514.
PRIDEiQ16514.

Protocols and materials databases

DNASUi6883.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263974; ENSP00000263974; ENSG00000120656. [Q16514-1]
ENST00000373824; ENSP00000362930; ENSG00000120656. [Q16514-1]
GeneIDi6883.
KEGGihsa:6883.
UCSCiuc001bqx.5. human. [Q16514-1]

Organism-specific databases

CTDi6883.
DisGeNETi6883.
GeneCardsiTAF12.
HGNCiHGNC:11545. TAF12.
HPAiHPA008519.
MIMi600773. gene.
neXtProtiNX_Q16514.
OpenTargetsiENSG00000120656.
PharmGKBiPA36320.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1142. Eukaryota.
COG5624. LUCA.
GeneTreeiENSGT00390000002144.
HOGENOMiHOG000035092.
HOVERGENiHBG055174.
InParanoidiQ16514.
KOiK03126.
OMAiHSSMANN.
OrthoDBiEOG091G0Q1X.
PhylomeDBiQ16514.
TreeFamiTF323652.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120656-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-3214847. HATs acetylate histones.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiQ16514.

Miscellaneous databases

EvolutionaryTraceiQ16514.
GeneWikiiTAF12.
GenomeRNAii6883.
PROiQ16514.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120656.
CleanExiHS_TAF12.
HS_TAF15.
GenevisibleiQ16514. HS.

Family and domain databases

CDDicd07981. TAF12. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003228. TFIID_TAF12.
[Graphical view]
PfamiPF03847. TFIID_20kDa. 1 hit.
[Graphical view]
ProDomiPD012998. PD012998. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF12_HUMAN
AccessioniPrimary (citable) accession number: Q16514
Secondary accession number(s): D3DPM5, Q15775, Q5T077
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 178 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.