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Q16478 (GRIK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate receptor ionotropic, kainate 5

Short name=GluK5
Alternative name(s):
Excitatory amino acid receptor 2
Short name=EAA2
Glutamate receptor KA-2
Short name=KA2
Gene names
Name:GRIK5
Synonyms:GRIK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length980 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.

Subunit structure

Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

establishment of localization in cell

Inferred from electronic annotation. Source: Ensembl

ion transmembrane transport

Inferred from Biological aspect of Ancestor. Source: GOC

ionotropic glutamate receptor signaling pathway

Inferred from direct assay Ref.1. Source: GOC

positive regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

protein retention in ER lumen

Inferred from electronic annotation. Source: Ensembl

receptor clustering

Inferred from electronic annotation. Source: Ensembl

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of synaptic vesicle fusion to presynaptic membrane

Inferred from electronic annotation. Source: Ensembl

synaptic transmission

Traceable author statement. Source: Reactome

synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from Biological aspect of Ancestor. Source: RefGenome

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

kainate selective glutamate receptor complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

perikaryon

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

postsynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

presynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionextracellular-glutamate-gated ion channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

kainate selective glutamate receptor activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q16478-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q16478-2)

The sequence of this isoform differs from the canonical sequence as follows:
     839-980: VSVCQEMLQE...AGPRELAEHE → TPALHPAACQ...ATLVTSNFHV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 980966Glutamate receptor ionotropic, kainate 5
PRO_0000011552

Regions

Topological domain15 – 544530Extracellular Potential
Transmembrane545 – 56521Helical; Potential
Topological domain566 – 62257Cytoplasmic Potential
Transmembrane623 – 64321Helical; Potential
Topological domain644 – 803160Extracellular Potential
Transmembrane804 – 82421Helical; Potential
Topological domain825 – 980156Cytoplasmic Potential
Compositional bias860 – 8667Poly-Arg

Amino acid modifications

Glycosylation2191N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3721N-linked (GlcNAc...) Potential
Glycosylation3941N-linked (GlcNAc...) Potential
Glycosylation4001N-linked (GlcNAc...) Potential
Glycosylation4071N-linked (GlcNAc...) Potential
Glycosylation4141N-linked (GlcNAc...) Potential
Glycosylation4781N-linked (GlcNAc...) Potential
Disulfide bond36 ↔ 292 By similarity
Disulfide bond83 ↔ 334 By similarity
Disulfide bond165 ↔ 170 By similarity

Natural variations

Alternative sequence839 – 980142VSVCQ…LAEHE → TPALHPAACQCSALGPRTPL KEPSMLLVKVPSTRVQVAFS RTSLRQVCPFLLQHQLSSLY WIQATNVQICCHFSSLKPSP DLTFPPSHRPLSSLLFTALA AVGGLPDASSFFFPPISSCP PLQSGIGPCHSTEATLVTSN FHV in isoform 2.
VSP_035585
Natural variant5271V → M.
Corresponds to variant rs2230298 [ dbSNP | Ensembl ].
VAR_047000

Experimental info

Sequence conflict4361A → G in AAB22591. Ref.1
Sequence conflict459 – 4613RFR → PFP in AAB22591. Ref.1
Sequence conflict7111R → A in AAB22591. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 72466FFF64079388

FASTA980109,265
        10         20         30         40         50         60 
MPAELLLLLI VAFASPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ INGIIEVPAK 

        70         80         90        100        110        120 
ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP ASASTVSHIC GEKEIPHIKV 

       130        140        150        160        170        180 
GPEETPRLQY LRFASVSLYP SNEDVSLAVS RILKSFNYPS ASLICAKAEC LLRLEELVRG 

       190        200        210        220        230        240 
FLISKETLSV RMLDDSRDPT PLLKEIRDDK VSTIIIDANA SISHLILRKA SELGMTSAFY 

       250        260        270        280        290        300 
KYILTTMDFP ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYLGP 

       310        320        330        340        350        360 
ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL RMVEYDGLTG 

       370        380        390        400        410        420 
RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN ATTLDINLSQ TLANKTLVVT 

       430        440        450        460        470        480 
TILENPYVMR RPNFQALSGN ERFEGFCVDM LRELAELLRF RYRLRLVEDG LYGAPEPNGS 

       490        500        510        520        530        540 
WTGMVGELIN RKADLAVAAF TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD 

       550        560        570        580        590        600 
PFSPAVWLFM LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV 

       610        620        630        640        650        660 
GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV PVESADDLAD 

       670        680        690        700        710        720 
QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV FVKSTEEGIA RVLNSRYAFL 

       730        740        750        760        770        780 
LESTMNEYHR RLNCNLTQIG GLLDTKGYGI GMPLGSPFRD EITLAILQLQ ENNRLEILKR 

       790        800        810        820        830        840 
KWWEGGRCPK EEDHRAKGLG MENIGGIFIV LICGLIIAVF VAVMEFIWST RRSAESEEVS 

       850        860        870        880        890        900 
VCQEMLQELR HAVSCRKTSR SRRRRRPGGP SRALLSLRAV REMRLSNGKL YSAGAGGDAG 

       910        920        930        940        950        960 
SAHGGPQRLL DDPGPPSGAR PAAPTPCTHV RVCQECRRIQ ALRASGAGAP PRGLGVPAEA 

       970        980 
TSPPRPRPGP AGPRELAEHE 

« Hide

Isoform 2 [UniParc].

Checksum: 71DFC5DE362DF1C6
Show »

FASTA981109,658

References

« Hide 'large scale' references
[1]"Molecular structure and pharmacological characterization of humEAA2, a novel human kainate receptor subunit."
Kamboj R.K., Schoepp D.D., Nutt S., Shekter L., Korczak B., True R.A., Zimmerman D.M., Wosnick M.A.
Mol. Pharmacol. 42:10-15(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Hippocampus.
[2]"Myeloid progenitor cell growth and apoptosis involves known and cell-specific ionotropic glutamate receptors."
Langer A., Xu D., Kuehcke K., Fehse B., Abdallah S., Lother H.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Erythroleukemia.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S40369 mRNA. Translation: AAB22591.1.
AJ249209 mRNA. Translation: CAC80547.1.
CH471126 Genomic DNA. Translation: EAW57090.1.
PIRI57936.
RefSeqNP_002079.3. NM_002088.4.
XP_005258878.1. XM_005258821.1.
UniGeneHs.367799.

3D structure databases

ProteinModelPortalQ16478.
SMRQ16478. Positions 22-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109158. 7 interactions.
IntActQ16478. 4 interactions.
MINTMINT-1427286.
STRING9606.ENSP00000262895.

Chemistry

BindingDBQ16478.
ChEMBLCHEMBL2109241.
DrugBankDB00142. L-Glutamic Acid.
GuidetoPHARMACOLOGY454.

PTM databases

PhosphoSiteQ16478.

Polymorphism databases

DMDM209572626.

Proteomic databases

PaxDbQ16478.
PRIDEQ16478.

Protocols and materials databases

DNASU2901.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262895; ENSP00000262895; ENSG00000105737. [Q16478-1]
ENST00000301218; ENSP00000301218; ENSG00000105737. [Q16478-2]
ENST00000593562; ENSP00000470251; ENSG00000105737. [Q16478-1]
ENST00000607949; ENSP00000476282; ENSG00000272706. [Q16478-1]
ENST00000608156; ENSP00000477354; ENSG00000272706. [Q16478-1]
ENST00000609917; ENSP00000476376; ENSG00000272706. [Q16478-2]
GeneID2901.
KEGGhsa:2901.
UCSCuc002osi.2. human. [Q16478-1]

Organism-specific databases

CTD2901.
GeneCardsGC19M042502.
H-InvDBHIX0022359.
HIX0027521.
HGNCHGNC:4583. GRIK5.
MIM600283. gene.
neXtProtNX_Q16478.
PharmGKBPA28977.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG316680.
HOGENOMHOG000234371.
HOVERGENHBG051839.
KOK05205.
OMAGLNCNLT.
OrthoDBEOG71G9W6.
PhylomeDBQ16478.
TreeFamTF334668.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

ArrayExpressQ16478.
BgeeQ16478.
CleanExHS_GRIK2.
HS_GRIK5.
GenevestigatorQ16478.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
ProtoNetSearch...

Other

GeneWikiGRIK5.
GenomeRNAi2901.
NextBio11483.
PROQ16478.
SOURCESearch...

Entry information

Entry nameGRIK5_HUMAN
AccessionPrimary (citable) accession number: Q16478
Secondary accession number(s): Q8WWG8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 14, 2008
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM