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Protein

Glutamate receptor ionotropic, kainate 5

Gene

GRIK5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_21346. Activation of Ca-permeable Kainate Receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, kainate 5
Short name:
GluK5
Alternative name(s):
Excitatory amino acid receptor 2
Short name:
EAA2
Glutamate receptor KA-2
Short name:
KA2
Gene namesi
Name:GRIK5
Synonyms:GRIK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:4583. GRIK5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini15 – 544530ExtracellularSequence AnalysisAdd
BLAST
Transmembranei545 – 56521HelicalSequence AnalysisAdd
BLAST
Topological domaini566 – 62257CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei623 – 64321HelicalSequence AnalysisAdd
BLAST
Topological domaini644 – 803160ExtracellularSequence AnalysisAdd
BLAST
Transmembranei804 – 82421HelicalSequence AnalysisAdd
BLAST
Topological domaini825 – 980156CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28977.

Polymorphism and mutation databases

BioMutaiGRIK5.
DMDMi209572626.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence AnalysisAdd
BLAST
Chaini15 – 980966Glutamate receptor ionotropic, kainate 5PRO_0000011552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 292By similarity
Disulfide bondi83 ↔ 334By similarity
Disulfide bondi165 ↔ 170By similarity
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ16478.
PRIDEiQ16478.

PTM databases

PhosphoSiteiQ16478.

Expressioni

Gene expression databases

BgeeiQ16478.
CleanExiHS_GRIK2.
HS_GRIK5.
ExpressionAtlasiQ16478. baseline and differential.
GenevisibleiQ16478. HS.

Interactioni

Subunit structurei

Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi109158. 7 interactions.
IntActiQ16478. 4 interactions.
MINTiMINT-1427286.
STRINGi9606.ENSP00000262895.

Structurei

3D structure databases

ProteinModelPortaliQ16478.
SMRiQ16478. Positions 22-828.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi860 – 8667Poly-Arg

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG316680.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234371.
HOVERGENiHBG051839.
InParanoidiQ16478.
KOiK05205.
OMAiCTSSNIW.
OrthoDBiEOG71G9W6.
PhylomeDBiQ16478.
TreeFamiTF334668.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q16478-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAELLLLLI VAFASPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ
60 70 80 90 100
INGIIEVPAK ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP
110 120 130 140 150
ASASTVSHIC GEKEIPHIKV GPEETPRLQY LRFASVSLYP SNEDVSLAVS
160 170 180 190 200
RILKSFNYPS ASLICAKAEC LLRLEELVRG FLISKETLSV RMLDDSRDPT
210 220 230 240 250
PLLKEIRDDK VSTIIIDANA SISHLILRKA SELGMTSAFY KYILTTMDFP
260 270 280 290 300
ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYLGP
310 320 330 340 350
ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL
360 370 380 390 400
RMVEYDGLTG RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN
410 420 430 440 450
ATTLDINLSQ TLANKTLVVT TILENPYVMR RPNFQALSGN ERFEGFCVDM
460 470 480 490 500
LRELAELLRF RYRLRLVEDG LYGAPEPNGS WTGMVGELIN RKADLAVAAF
510 520 530 540 550
TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD PFSPAVWLFM
560 570 580 590 600
LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV
610 620 630 640 650
GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV
660 670 680 690 700
PVESADDLAD QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV
710 720 730 740 750
FVKSTEEGIA RVLNSRYAFL LESTMNEYHR RLNCNLTQIG GLLDTKGYGI
760 770 780 790 800
GMPLGSPFRD EITLAILQLQ ENNRLEILKR KWWEGGRCPK EEDHRAKGLG
810 820 830 840 850
MENIGGIFIV LICGLIIAVF VAVMEFIWST RRSAESEEVS VCQEMLQELR
860 870 880 890 900
HAVSCRKTSR SRRRRRPGGP SRALLSLRAV REMRLSNGKL YSAGAGGDAG
910 920 930 940 950
SAHGGPQRLL DDPGPPSGAR PAAPTPCTHV RVCQECRRIQ ALRASGAGAP
960 970 980
PRGLGVPAEA TSPPRPRPGP AGPRELAEHE
Length:980
Mass (Da):109,265
Last modified:October 14, 2008 - v2
Checksum:i72466FFF64079388
GO
Isoform 2 (identifier: Q16478-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     839-980: VSVCQEMLQE...AGPRELAEHE → TPALHPAACQ...ATLVTSNFHV

Note: No experimental confirmation available.
Show »
Length:981
Mass (Da):109,658
Checksum:i71DFC5DE362DF1C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4361A → G in AAB22591 (PubMed:1321949).Curated
Sequence conflicti459 – 4613RFR → PFP in AAB22591 (PubMed:1321949).Curated
Sequence conflicti711 – 7111R → A in AAB22591 (PubMed:1321949).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti527 – 5271V → M.
Corresponds to variant rs2230298 [ dbSNP | Ensembl ].
VAR_047000

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei839 – 980142VSVCQ…LAEHE → TPALHPAACQCSALGPRTPL KEPSMLLVKVPSTRVQVAFS RTSLRQVCPFLLQHQLSSLY WIQATNVQICCHFSSLKPSP DLTFPPSHRPLSSLLFTALA AVGGLPDASSFFFPPISSCP PLQSGIGPCHSTEATLVTSN FHV in isoform 2. 1 PublicationVSP_035585Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S40369 mRNA. Translation: AAB22591.1.
AJ249209 mRNA. Translation: CAC80547.1.
CH471126 Genomic DNA. Translation: EAW57090.1.
CCDSiCCDS12595.1. [Q16478-1]
CCDS77305.1. [Q16478-2]
PIRiI57936.
RefSeqiNP_001287959.1. NM_001301030.1. [Q16478-2]
NP_002079.3. NM_002088.4. [Q16478-1]
XP_005258878.1. XM_005258821.2. [Q16478-1]
XP_011525165.1. XM_011526863.1. [Q16478-2]
UniGeneiHs.367799.

Genome annotation databases

EnsembliENST00000262895; ENSP00000262895; ENSG00000105737.
ENST00000301218; ENSP00000301218; ENSG00000105737. [Q16478-2]
ENST00000593562; ENSP00000470251; ENSG00000105737.
GeneIDi2901.
KEGGihsa:2901.
UCSCiuc002osi.2. human. [Q16478-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S40369 mRNA. Translation: AAB22591.1.
AJ249209 mRNA. Translation: CAC80547.1.
CH471126 Genomic DNA. Translation: EAW57090.1.
CCDSiCCDS12595.1. [Q16478-1]
CCDS77305.1. [Q16478-2]
PIRiI57936.
RefSeqiNP_001287959.1. NM_001301030.1. [Q16478-2]
NP_002079.3. NM_002088.4. [Q16478-1]
XP_005258878.1. XM_005258821.2. [Q16478-1]
XP_011525165.1. XM_011526863.1. [Q16478-2]
UniGeneiHs.367799.

3D structure databases

ProteinModelPortaliQ16478.
SMRiQ16478. Positions 22-828.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109158. 7 interactions.
IntActiQ16478. 4 interactions.
MINTiMINT-1427286.
STRINGi9606.ENSP00000262895.

Chemistry

BindingDBiQ16478.
ChEMBLiCHEMBL2109241.
GuidetoPHARMACOLOGYi454.

PTM databases

PhosphoSiteiQ16478.

Polymorphism and mutation databases

BioMutaiGRIK5.
DMDMi209572626.

Proteomic databases

PaxDbiQ16478.
PRIDEiQ16478.

Protocols and materials databases

DNASUi2901.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262895; ENSP00000262895; ENSG00000105737.
ENST00000301218; ENSP00000301218; ENSG00000105737. [Q16478-2]
ENST00000593562; ENSP00000470251; ENSG00000105737.
GeneIDi2901.
KEGGihsa:2901.
UCSCiuc002osi.2. human. [Q16478-1]

Organism-specific databases

CTDi2901.
GeneCardsiGC19M042502.
H-InvDBHIX0022359.
HIX0027521.
HGNCiHGNC:4583. GRIK5.
MIMi600283. gene.
neXtProtiNX_Q16478.
PharmGKBiPA28977.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG316680.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234371.
HOVERGENiHBG051839.
InParanoidiQ16478.
KOiK05205.
OMAiCTSSNIW.
OrthoDBiEOG71G9W6.
PhylomeDBiQ16478.
TreeFamiTF334668.

Enzyme and pathway databases

ReactomeiREACT_21346. Activation of Ca-permeable Kainate Receptor.

Miscellaneous databases

GeneWikiiGRIK5.
GenomeRNAii2901.
NextBioi11483.
PROiQ16478.
SOURCEiSearch...

Gene expression databases

BgeeiQ16478.
CleanExiHS_GRIK2.
HS_GRIK5.
ExpressionAtlasiQ16478. baseline and differential.
GenevisibleiQ16478. HS.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular structure and pharmacological characterization of humEAA2, a novel human kainate receptor subunit."
    Kamboj R.K., Schoepp D.D., Nutt S., Shekter L., Korczak B., True R.A., Zimmerman D.M., Wosnick M.A.
    Mol. Pharmacol. 42:10-15(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Hippocampus.
  2. "Myeloid progenitor cell growth and apoptosis involves known and cell-specific ionotropic glutamate receptors."
    Langer A., Xu D., Kuehcke K., Fehse B., Abdallah S., Lother H.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Erythroleukemia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiGRIK5_HUMAN
AccessioniPrimary (citable) accession number: Q16478
Secondary accession number(s): Q8WWG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 14, 2008
Last modified: July 22, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.