Q16478 (GRIK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor ionotropic, kainate 5
Short name=GluK5

Alternative name(s):
Excitatory amino acid receptor 2
Short name=EAA2
Glutamate receptor KA-2
Short name=KA2

Gene names

Name:	GRIK5
Synonyms:	GRIK2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > domoate > L-glutamate >> AMPA >> NMDA = 1S,3R-ACPD.
Subunit structure	Tetramer of two or more different subunits. Associates with GRIK1 (both edited and unedited versions), GRIK2, or GRIK3 to form functional channels. Homomeric associations do not produce any channel activity By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK5 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to glucose stimulus Inferred from electronic annotation. Source: Compara establishment of localization in cell Inferred from electronic annotation. Source: Compara positive regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara protein retention in ER lumen Inferred from electronic annotation. Source: Compara receptor clustering Inferred from electronic annotation. Source: Compara regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of synaptic vesicle fusion to presynaptic membrane Inferred from electronic annotation. Source: Compara synaptic transmission Non-traceable author statement. Source: UniProtKB synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Non-traceable author statement. Source: UniProtKB kainate selective glutamate receptor complex Inferred from electronic annotation. Source: Compara perikaryon Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement. Source: Reactome postsynaptic density Inferred from electronic annotation. Source: Compara postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic membrane Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	extracellular-glutamate-gated ion channel activity Inferred from electronic annotation. Source: InterPro kainate selective glutamate receptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q16478-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q16478-2)

The sequence of this isoform differs from the canonical sequence as follows:
     839-980: VSVCQEMLQE...AGPRELAEHE → TPALHPAACQ...ATLVTSNFHV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 14	14	Potential
	Chain	15 – 980	966	Glutamate receptor ionotropic, kainate 5		PRO_0000011552
Regions
	Topological domain	15 – 544	530	Extracellular Potential
	Transmembrane	545 – 565	21	Helical; Potential
	Topological domain	566 – 622	57	Cytoplasmic Potential
	Transmembrane	623 – 643	21	Helical; Potential
	Topological domain	644 – 803	160	Extracellular Potential
	Transmembrane	804 – 824	21	Helical; Potential
	Topological domain	825 – 980	156	Cytoplasmic Potential
	Compositional bias	860 – 866	7	Poly-Arg
Amino acid modifications
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	372	1	N-linked (GlcNAc...) Potential
	Glycosylation	394	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	407	1	N-linked (GlcNAc...) Potential
	Glycosylation	414	1	N-linked (GlcNAc...) Potential
	Glycosylation	478	1	N-linked (GlcNAc...) Potential
	Disulfide bond	36 ↔ 292		By similarity
	Disulfide bond	83 ↔ 334		By similarity
	Disulfide bond	165 ↔ 170		By similarity
Natural variations
	Alternative sequence	839 – 980	142	VSVCQ…LAEHE → TPALHPAACQCSALGPRTPL KEPSMLLVKVPSTRVQVAFS RTSLRQVCPFLLQHQLSSLY WIQATNVQICCHFSSLKPSP DLTFPPSHRPLSSLLFTALA AVGGLPDASSFFFPPISSCP PLQSGIGPCHSTEATLVTSN FHV in isoform 2.		VSP_035585
	Natural variant	527	1	V → M. Corresponds to variant rs2230298 [ dbSNP | Ensembl ].		VAR_047000
Experimental info
	Sequence conflict	436	1	A → G in AAB22591. Ref.1
	Sequence conflict	459 – 461	3	RFR → PFP in AAB22591. Ref.1
	Sequence conflict	711	1	R → A in AAB22591. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 72466FFF64079388	FASTA	980	109,265
	10 20 30 40 50 60 MPAELLLLLI VAFASPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ INGIIEVPAK 70 80 90 100 110 120 ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP ASASTVSHIC GEKEIPHIKV 130 140 150 160 170 180 GPEETPRLQY LRFASVSLYP SNEDVSLAVS RILKSFNYPS ASLICAKAEC LLRLEELVRG 190 200 210 220 230 240 FLISKETLSV RMLDDSRDPT PLLKEIRDDK VSTIIIDANA SISHLILRKA SELGMTSAFY 250 260 270 280 290 300 KYILTTMDFP ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYLGP 310 320 330 340 350 360 ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL RMVEYDGLTG 370 380 390 400 410 420 RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN ATTLDINLSQ TLANKTLVVT 430 440 450 460 470 480 TILENPYVMR RPNFQALSGN ERFEGFCVDM LRELAELLRF RYRLRLVEDG LYGAPEPNGS 490 500 510 520 530 540 WTGMVGELIN RKADLAVAAF TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD 550 560 570 580 590 600 PFSPAVWLFM LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV 610 620 630 640 650 660 GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV PVESADDLAD 670 680 690 700 710 720 QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV FVKSTEEGIA RVLNSRYAFL 730 740 750 760 770 780 LESTMNEYHR RLNCNLTQIG GLLDTKGYGI GMPLGSPFRD EITLAILQLQ ENNRLEILKR 790 800 810 820 830 840 KWWEGGRCPK EEDHRAKGLG MENIGGIFIV LICGLIIAVF VAVMEFIWST RRSAESEEVS 850 860 870 880 890 900 VCQEMLQELR HAVSCRKTSR SRRRRRPGGP SRALLSLRAV REMRLSNGKL YSAGAGGDAG 910 920 930 940 950 960 SAHGGPQRLL DDPGPPSGAR PAAPTPCTHV RVCQECRRIQ ALRASGAGAP PRGLGVPAEA 970 980 TSPPRPRPGP AGPRELAEHE
	Isoform 2 [UniParc]. Checksum: 71DFC5DE362DF1C6	FASTA	981	109,658
	10 20 30 40 50 60 MPAELLLLLI VAFASPSCQV LSSLRMAAIL DDQTVCGRGE RLALALAREQ INGIIEVPAK 70 80 90 100 110 120 ARVEVDIFEL QRDSQYETTD TMCQILPKGV VSVLGPSSSP ASASTVSHIC GEKEIPHIKV 130 140 150 160 170 180 GPEETPRLQY LRFASVSLYP SNEDVSLAVS RILKSFNYPS ASLICAKAEC LLRLEELVRG 190 200 210 220 230 240 FLISKETLSV RMLDDSRDPT PLLKEIRDDK VSTIIIDANA SISHLILRKA SELGMTSAFY 250 260 270 280 290 300 KYILTTMDFP ILHLDGIVED SSNILGFSMF NTSHPFYPEF VRSLNMSWRE NCEASTYLGP 310 320 330 340 350 360 ALSAALMFDA VHVVVSAVRE LNRSQEIGVK PLACTSANIW PHGTSLMNYL RMVEYDGLTG 370 380 390 400 410 420 RVEFNSKGQR TNYTLRILEK SRQGHREIGV WYSNRTLAMN ATTLDINLSQ TLANKTLVVT 430 440 450 460 470 480 TILENPYVMR RPNFQALSGN ERFEGFCVDM LRELAELLRF RYRLRLVEDG LYGAPEPNGS 490 500 510 520 530 540 WTGMVGELIN RKADLAVAAF TITAEREKVI DFSKPFMTLG ISILYRVHMG RKPGYFSFLD 550 560 570 580 590 600 PFSPAVWLFM LLAYLAVSCV LFLAARLSPY EWYNPHPCLR ARPHILENQY TLGNSLWFPV 610 620 630 640 650 660 GGFMQQGSEI MPRALSTRCV SGVWWAFTLI IISSYTANLA AFLTVQRMEV PVESADDLAD 670 680 690 700 710 720 QTNIEYGTIH AGSTMTFFQN SRYQTYQRMW NYMQSKQPSV FVKSTEEGIA RVLNSRYAFL 730 740 750 760 770 780 LESTMNEYHR RLNCNLTQIG GLLDTKGYGI GMPLGSPFRD EITLAILQLQ ENNRLEILKR 790 800 810 820 830 840 KWWEGGRCPK EEDHRAKGLG MENIGGIFIV LICGLIIAVF VAVMEFIWST RRSAESEETP 850 860 870 880 890 900 ALHPAACQCS ALGPRTPLKE PSMLLVKVPS TRVQVAFSRT SLRQVCPFLL QHQLSSLYWI 910 920 930 940 950 960 QATNVQICCH FSSLKPSPDL TFPPSHRPLS SLLFTALAAV GGLPDASSFF FPPISSCPPL 970 980 QSGIGPCHST EATLVTSNFH V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular structure and pharmacological characterization of humEAA2, a novel human kainate receptor subunit." Kamboj R.K., Schoepp D.D., Nutt S., Shekter L., Korczak B., True R.A., Zimmerman D.M., Wosnick M.A. Mol. Pharmacol. 42:10-15(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hippocampus.
[2]	"Myeloid progenitor cell growth and apoptosis involves known and cell-specific ionotropic glutamate receptors." Langer A., Xu D., Kuehcke K., Fehse B., Abdallah S., Lother H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Erythroleukemia.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S40369 mRNA. Translation: AAB22591.1. AJ249209 mRNA. Translation: CAC80547.1. CH471126 Genomic DNA. Translation: EAW57090.1.
IPI	IPI00103335. IPI00293288.
PIR	I57936.
RefSeq	NP_002079.3. NM_002088.4.
UniGene	Hs.367799.
3D structure databases
ProteinModelPortal	Q16478.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q16478. 4 interactions.
MINT	MINT-1427286.
STRING	9606.ENSP00000262895.
PTM databases
PhosphoSite	Q16478.
Polymorphism databases
DMDM	209572626.
Proteomic databases
PaxDb	Q16478.
PRIDE	Q16478.
Protocols and materials databases
DNASU	2901.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262895; ENSP00000262895; ENSG00000105737. ENST00000301218; ENSP00000301218; ENSG00000105737. ENST00000593562; ENSP00000470251; ENSG00000105737.
GeneID	2901.
KEGG	hsa:2901.
UCSC	uc002osi.1. human.
Organism-specific databases
CTD	2901.
GeneCards	GC19M042502.
H-InvDB	HIX0022359. HIX0027521.
HGNC	HGNC:4583. GRIK5.
MIM	600283. gene.
neXtProt	NX_Q16478.
PharmGKB	PA28977.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG316680.
HOGENOM	HOG000234371.
HOVERGEN	HBG051839.
KO	K05205.
OMA	NINSVME.
OrthoDB	EOG4RV2QP.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
Bgee	Q16478.
CleanEx	HS_GRIK2. HS_GRIK5.
Genevestigator	Q16478.
GermOnline	ENSG00000105737. Homo sapiens.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR019594. Glu_rcpt_Glu/Gly-bd. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. [Graphical view]
Pfam	PF01094. ANF_receptor. 1 hit. PF00060. Lig_chan. 1 hit. PF10613. Lig_chan-Glu_bd. 1 hit. [Graphical view]
PRINTS	PR00177. NMDARECEPTOR.
SMART	SM00918. Lig_chan-Glu_bd. 1 hit. SM00079. PBPe. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q16478.
ChEMBL	CHEMBL2675.
DrugBank	DB00142. L-Glutamic Acid.
GenomeRNAi	2901.
NextBio	11483.
SOURCE	Search...

Entry information

Entry name

GRIK5_HUMAN

Accession

Primary (citable) accession number: Q16478
Secondary accession number(s): Q8WWG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 14, 2008
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.