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Protein

Alpha-internexin

Gene

INA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148798-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-internexin
Short name:
Alpha-Inx
Alternative name(s):
66 kDa neurofilament protein
Short name:
NF-66
Short name:
Neurofilament-66
Neurofilament 5
Gene namesi
Name:INA
Synonyms:NEF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:6057. INA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic ribonucleoprotein granule Source: ParkinsonsUK-UCL
  • extracellular space Source: UniProtKB
  • intermediate filament cytoskeleton Source: HPA
  • myelin sheath Source: Ensembl
  • neurofilament Source: ProtInc
  • nuclear membrane Source: HPA
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

Pathology & Biotechi

Organism-specific databases

DisGeNETi9118.
OpenTargetsiENSG00000148798.
PharmGKBiPA29867.

Polymorphism and mutation databases

BioMutaiINA.
DMDMi20141266.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637831 – 499Alpha-internexinAdd BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei290N6-acetyllysineCombined sources1
Modified residuei335PhosphoserineBy similarity1
Modified residuei469PhosphoserineBy similarity1
Modified residuei496PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ16352.
MaxQBiQ16352.
PaxDbiQ16352.
PeptideAtlasiQ16352.
PRIDEiQ16352.

PTM databases

iPTMnetiQ16352.
PhosphoSitePlusiQ16352.
SwissPalmiQ16352.

Expressioni

Tissue specificityi

Found predominantly in adult CNS.

Developmental stagei

Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.

Gene expression databases

BgeeiENSG00000148798.
CleanExiHS_INA.
GenevisibleiQ16352. HS.

Organism-specific databases

HPAiCAB002059.
HPA008057.

Interactioni

Protein-protein interaction databases

BioGridi114566. 36 interactors.
IntActiQ16352. 7 interactors.
STRINGi9606.ENSP00000358865.

Structurei

3D structure databases

ProteinModelPortaliQ16352.
SMRiQ16352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 87HeadAdd BLAST87
Regioni88 – 408RodAdd BLAST321
Regioni88 – 129Coil 1AAdd BLAST42
Regioni130 – 142Linker 1Add BLAST13
Regioni143 – 238Coil 1BAdd BLAST96
Regioni239 – 262Linker 2Add BLAST24
Regioni263 – 408Coil 2Add BLAST146
Regioni409 – 499TailAdd BLAST91

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi449 – 454Poly-Glu6

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ16352.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG091G12MK.
PhylomeDBiQ16352.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS
60 70 80 90 100
SAACSSASSL GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELRDL
160 170 180 190 200
RAQLEEASSA RSQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSATTSKVS STGLSLKKEE
460 470 480 490
EEEEASKVAS KKTSQIGESF EEILEETVIS TKKTEKSNIE ETTISSQKI
Length:499
Mass (Da):55,391
Last modified:January 23, 2002 - v2
Checksum:i4C972764E9E68D3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 41GFRSQ → ASVE in AAB34482 (PubMed:7769995).Curated5
Sequence conflicti67R → A in AAB34482 (PubMed:7769995).Curated1
Sequence conflicti128 – 132ALRQR → RCDT in AAB34482 (PubMed:7769995).Curated5
Sequence conflicti141E → Q in AAB34482 (PubMed:7769995).Curated1
Sequence conflicti147 – 152LRDLRA → PRHLP in AAB34482 (PubMed:7769995).Curated6
Sequence conflicti191 – 198GAERALKA → RRARLKR in AAB34482 (PubMed:7769995).Curated8
Sequence conflicti244A → R in AAB34482 (PubMed:7769995).Curated1
Sequence conflicti263S → A in AAB34482 (PubMed:7769995).Curated1
Sequence conflicti301S → T in AAB34482 (PubMed:7769995).Curated1
Sequence conflicti310 – 311EE → DQ in AAB34482 (PubMed:7769995).Curated2
Sequence conflicti318Missing in AAB34482 (PubMed:7769995).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04980892T → S.1 PublicationCorresponds to variant rs1063455dbSNPEnsembl.1
Natural variantiVAR_036369110E → Q in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_033497149D → H.Corresponds to variant rs1063456dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78296 mRNA. Translation: AAB34482.1.
AL591408 Genomic DNA. Translation: CAI16744.1.
CH471066 Genomic DNA. Translation: EAW49653.1.
BC006359 mRNA. Translation: AAH06359.1.
CCDSiCCDS7545.1.
PIRiI52658.
RefSeqiNP_116116.1. NM_032727.3.
UniGeneiHs.500916.

Genome annotation databases

EnsembliENST00000369849; ENSP00000358865; ENSG00000148798.
GeneIDi9118.
KEGGihsa:9118.
UCSCiuc001kws.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S78296 mRNA. Translation: AAB34482.1.
AL591408 Genomic DNA. Translation: CAI16744.1.
CH471066 Genomic DNA. Translation: EAW49653.1.
BC006359 mRNA. Translation: AAH06359.1.
CCDSiCCDS7545.1.
PIRiI52658.
RefSeqiNP_116116.1. NM_032727.3.
UniGeneiHs.500916.

3D structure databases

ProteinModelPortaliQ16352.
SMRiQ16352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114566. 36 interactors.
IntActiQ16352. 7 interactors.
STRINGi9606.ENSP00000358865.

PTM databases

iPTMnetiQ16352.
PhosphoSitePlusiQ16352.
SwissPalmiQ16352.

Polymorphism and mutation databases

BioMutaiINA.
DMDMi20141266.

Proteomic databases

EPDiQ16352.
MaxQBiQ16352.
PaxDbiQ16352.
PeptideAtlasiQ16352.
PRIDEiQ16352.

Protocols and materials databases

DNASUi9118.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369849; ENSP00000358865; ENSG00000148798.
GeneIDi9118.
KEGGihsa:9118.
UCSCiuc001kws.3. human.

Organism-specific databases

CTDi9118.
DisGeNETi9118.
GeneCardsiINA.
HGNCiHGNC:6057. INA.
HPAiCAB002059.
HPA008057.
MIMi605338. gene.
neXtProtiNX_Q16352.
OpenTargetsiENSG00000148798.
PharmGKBiPA29867.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ16352.
KOiK07608.
OMAiASSYRKV.
OrthoDBiEOG091G12MK.
PhylomeDBiQ16352.
TreeFamiTF330122.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148798-MONOMER.

Miscellaneous databases

ChiTaRSiINA. human.
GenomeRNAii9118.
PROiQ16352.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148798.
CleanExiHS_INA.
GenevisibleiQ16352. HS.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAINX_HUMAN
AccessioniPrimary (citable) accession number: Q16352
Secondary accession number(s): B1AQK0, Q9BRC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2002
Last modified: November 30, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.