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Q16352

- AINX_HUMAN

UniProt

Q16352 - AINX_HUMAN

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Protein

Alpha-internexin

Gene
INA, NEF5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

GO - Molecular functioni

  1. structural constituent of cytoskeleton Source: ProtInc

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. neurofilament cytoskeleton organization Source: Ensembl
  3. substantia nigra development Source: UniProt
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-internexin
Short name:
Alpha-Inx
Alternative name(s):
66 kDa neurofilament protein
Short name:
NF-66
Short name:
Neurofilament-66
Neurofilament 5
Gene namesi
Name:INA
Synonyms:NEF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:6057. INA.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProt
  2. neurofilament Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29867.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Alpha-internexinPRO_0000063783Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721Phosphoserine By similarity
Modified residuei290 – 2901N6-acetyllysine1 Publication
Modified residuei335 – 3351Phosphoserine By similarity
Modified residuei496 – 4961Phosphoserine1 Publication

Post-translational modificationi

O-glycosylated By similarity.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ16352.
PaxDbiQ16352.
PeptideAtlasiQ16352.
PRIDEiQ16352.

PTM databases

PhosphoSiteiQ16352.

Expressioni

Tissue specificityi

Found predominantly in adult CNS.

Developmental stagei

Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.

Gene expression databases

ArrayExpressiQ16352.
BgeeiQ16352.
CleanExiHS_INA.
GenevestigatoriQ16352.

Organism-specific databases

HPAiCAB002059.
HPA008057.

Interactioni

Protein-protein interaction databases

BioGridi114566. 9 interactions.
IntActiQ16352. 3 interactions.
STRINGi9606.ENSP00000358865.

Structurei

3D structure databases

ProteinModelPortaliQ16352.
SMRiQ16352. Positions 91-231, 259-329, 333-402.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8787HeadAdd
BLAST
Regioni88 – 408321RodAdd
BLAST
Regioni88 – 12942Coil 1AAdd
BLAST
Regioni130 – 14213Linker 1Add
BLAST
Regioni143 – 23896Coil 1BAdd
BLAST
Regioni239 – 26224Linker 2Add
BLAST
Regioni263 – 408146Coil 2Add
BLAST
Regioni409 – 49991TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi449 – 4546Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149366.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ16352.
KOiK07608.
OMAiRCNVASS.
OrthoDBiEOG7FV3Q8.
PhylomeDBiQ16352.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q16352-1 [UniParc]FASTAAdd to Basket

« Hide

MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS    50
SAACSSASSL GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG 100
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELRDL 150
RAQLEEASSA RSQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ 200
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA 250
EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR 300
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE 350
VAGYQDSIGQ LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL 400
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSATTSKVS STGLSLKKEE 450
EEEEASKVAS KKTSQIGESF EEILEETVIS TKKTEKSNIE ETTISSQKI 499
Length:499
Mass (Da):55,391
Last modified:January 23, 2002 - v2
Checksum:i4C972764E9E68D3E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921T → S.1 Publication
Corresponds to variant rs1063455 [ dbSNP | Ensembl ].
VAR_049808
Natural varianti110 – 1101E → Q in a breast cancer sample; somatic mutation. 1 Publication
VAR_036369
Natural varianti149 – 1491D → H.
Corresponds to variant rs1063456 [ dbSNP | Ensembl ].
VAR_033497

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 415GFRSQ → ASVE in AAB34482. 1 Publication
Sequence conflicti67 – 671R → A in AAB34482. 1 Publication
Sequence conflicti128 – 1325ALRQR → RCDT in AAB34482. 1 Publication
Sequence conflicti141 – 1411E → Q in AAB34482. 1 Publication
Sequence conflicti147 – 1526LRDLRA → PRHLP in AAB34482. 1 Publication
Sequence conflicti191 – 1988GAERALKA → RRARLKR in AAB34482. 1 Publication
Sequence conflicti244 – 2441A → R in AAB34482. 1 Publication
Sequence conflicti263 – 2631S → A in AAB34482. 1 Publication
Sequence conflicti301 – 3011S → T in AAB34482. 1 Publication
Sequence conflicti310 – 3112EE → DQ in AAB34482. 1 Publication
Sequence conflicti318 – 3181Missing in AAB34482. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S78296 mRNA. Translation: AAB34482.1.
AL591408 Genomic DNA. Translation: CAI16744.1.
CH471066 Genomic DNA. Translation: EAW49653.1.
BC006359 mRNA. Translation: AAH06359.1.
CCDSiCCDS7545.1.
PIRiI52658.
RefSeqiNP_116116.1. NM_032727.3.
UniGeneiHs.500916.

Genome annotation databases

EnsembliENST00000369849; ENSP00000358865; ENSG00000148798.
GeneIDi9118.
KEGGihsa:9118.
UCSCiuc001kws.3. human.

Polymorphism databases

DMDMi20141266.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S78296 mRNA. Translation: AAB34482.1 .
AL591408 Genomic DNA. Translation: CAI16744.1 .
CH471066 Genomic DNA. Translation: EAW49653.1 .
BC006359 mRNA. Translation: AAH06359.1 .
CCDSi CCDS7545.1.
PIRi I52658.
RefSeqi NP_116116.1. NM_032727.3.
UniGenei Hs.500916.

3D structure databases

ProteinModelPortali Q16352.
SMRi Q16352. Positions 91-231, 259-329, 333-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114566. 9 interactions.
IntActi Q16352. 3 interactions.
STRINGi 9606.ENSP00000358865.

PTM databases

PhosphoSitei Q16352.

Polymorphism databases

DMDMi 20141266.

Proteomic databases

MaxQBi Q16352.
PaxDbi Q16352.
PeptideAtlasi Q16352.
PRIDEi Q16352.

Protocols and materials databases

DNASUi 9118.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000369849 ; ENSP00000358865 ; ENSG00000148798 .
GeneIDi 9118.
KEGGi hsa:9118.
UCSCi uc001kws.3. human.

Organism-specific databases

CTDi 9118.
GeneCardsi GC10P105026.
HGNCi HGNC:6057. INA.
HPAi CAB002059.
HPA008057.
MIMi 605338. gene.
neXtProti NX_Q16352.
PharmGKBi PA29867.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG149366.
HOGENOMi HOG000230977.
HOVERGENi HBG013015.
InParanoidi Q16352.
KOi K07608.
OMAi RCNVASS.
OrthoDBi EOG7FV3Q8.
PhylomeDBi Q16352.
TreeFami TF330122.

Miscellaneous databases

ChiTaRSi INA. human.
GenomeRNAii 9118.
NextBioi 34171.
PROi Q16352.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q16352.
Bgeei Q16352.
CleanExi HS_INA.
Genevestigatori Q16352.

Family and domain databases

InterProi IPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view ]
PANTHERi PTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
Pfami PF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view ]
PROSITEi PS00226. IF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and developmental expression of human 66 kd neurofilament protein."
    Chan S.-O., Chiu F.-C.
    Brain Res. Mol. Brain Res. 29:177-184(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-92.
    Tissue: Fetal brain.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 46-83; 105-111; 121-130; 139-145; 216-228; 279-288; 323-330; 339-367; 378-397 AND 407-430, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-496, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-110.

Entry informationi

Entry nameiAINX_HUMAN
AccessioniPrimary (citable) accession number: Q16352
Secondary accession number(s): B1AQK0, Q9BRC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2002
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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