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Q162K5 (THIM_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:RD1_3610
OrganismRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier375451 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRoseobacter

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000336569

Sites

Binding site421Substrate; via amide nitrogen By similarity
Binding site1171ATP By similarity
Binding site1631ATP By similarity
Binding site1901Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q162K5 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: CA5B990BE05FA6EF

FASTA25726,310
        10         20         30         40         50         60 
MAPHDLLTAL RDQNPLVHCI TNYVAMNIAA NVVLAAGASP AMVHAPEEIA EFTPICGALT 

        70         80         90        100        110        120 
INIGTISTPW QASMMAAAAT ASAQNIPWVL DPVAHFISVY RREAAQQLLA QRPTILRGNA 

       130        140        150        160        170        180 
SEILALTGET GAGKGADSGD SVDAAQGAAK KLAAQFGTVV AITGPVDYLT DGTREAQVSG 

       190        200        210        220        230        240 
GSSLMPQVTA LGCSQTALMG AYAATGPAFD AALAALAHFK VAGSTAAQRA DGPGSFQMHF 

       250 
LDALANTQPF ELAQVIR 

« Hide

References

[1]"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism."
Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W.
J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000362 Genomic DNA. Translation: ABG33088.1.
RefSeqYP_683774.1. NC_008209.1.

3D structure databases

ProteinModelPortalQ162K5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING375451.RD1_3610.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG33088; ABG33088; RD1_3610.
GeneID4195087.
KEGGrde:RD1_3610.
PATRIC23365175. VBIRosDen86677_3453.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMANACTQLA.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycRDEN375451:GJIZ-3404-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_ROSDO
AccessionPrimary (citable) accession number: Q162K5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways