Q162H5 (Q162H5_ROSDO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase HAMAP MF_01265
EC=1.4.1.21 HAMAP MF_01265

Gene names

Name:	nadX HAMAP MF_01265
Ordered Locus Names:	RD1_3642

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP] EMBL ABG33118.1

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family. HAMAP MF_01265

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis HAMAP MF_01265
Ligand	NAD HAMAP MF_01265 NADP HAMAP MF_01265
Molecular function	Oxidoreductase HAMAP MF_01265
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	213	1	By similarity HAMAP MF_01265
	Binding site	117	1	NAD; via amide nitrogen By similarity HAMAP MF_01265
	Binding site	183	1	NAD By similarity HAMAP MF_01265

Sequences

	Sequence		Length	Mass (Da)
	Q162H5 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 36B7B959E76A1F88	FASTA	260	27,170
	10 20 30 40 50 60 MKIGIIGEGA IGRYVMDQLD RDGRAPVVSL VRPQRLIGAQ DTGMTRVFHV CDLPPGLDLM 70 80 90 100 110 120 VDCAGHAALI EHGAAILKRG IDLVTVSLGA LADQHVEQSL RQAALTGGAQ LHLASGAIGA 130 140 150 160 170 180 LDCLQAARVG GLSAVRYTGR KPPAGWRGSA AQERVDLDML TTAAAHFEGT ARQAARQYPK 190 200 210 220 230 240 NANVAAAVAI AGLGFERTRV TLIADPDVTA NIHEIEASGA FGRFSFRIEG TALPDNPRSS 250 260 ALAAMSVISA IARRRAPVTT

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000362 Genomic DNA. Translation: ABG33118.1.
RefSeq	YP_683804.1. NC_008209.1.
3D structure databases
ProteinModelPortal	Q162H5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q162H5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4195484.
GenomeReviews	Gene locus RD1_3642 in contig CP000362_GR.
KEGG	rde:RD1_3642.
NMPDR	fig|375451.6.peg.3388.
PATRIC	23365237. VBIRosDen86677_3484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13303.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_3642-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q162H5_ROSDO

Accession

Primary (citable) accession number: Q162H5

Entry history

Integrated into UniProtKB/TrEMBL:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)