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Protein

Proteasomal ubiquitin receptor ADRM1

Gene

ADRM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Within the complex, functions as a proteasomal ubiquitin receptor. Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation. UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex.8 Publications

GO - Molecular functioni

  • endopeptidase activator activity Source: UniProtKB
  • protease binding Source: UniProtKB
  • proteasome binding Source: UniProtKB
  • ubiquitin binding Source: GO_Central

GO - Biological processi

  • proteasome assembly Source: UniProtKB
  • protein deubiquitination Source: Reactome
  • transcription elongation from RNA polymerase II promoter Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: GO_Central

Enzyme and pathway databases

ReactomeiR-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasomal ubiquitin receptor ADRM1
Alternative name(s):
110 kDa cell membrane glycoprotein
Short name:
Gp110
Adhesion-regulating molecule 1
Short name:
ARM-1
Proteasome regulatory particle non-ATPase 13
Short name:
hRpn13
Rpn13 homolog
Gene namesi
Name:ADRM1
Synonyms:GP110
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000130706.12.
HGNCiHGNC:15759. ADRM1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi11047.
OpenTargetsiENSG00000130706.
PharmGKBiPA24599.

Chemistry databases

ChEMBLiCHEMBL2364701.

Polymorphism and mutation databases

DMDMi20141265.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000206312 – 407Proteasomal ubiquitin receptor ADRM1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Cross-linki34Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei127PhosphotyrosineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei211PhosphoserineCombined sources1
Modified residuei217PhosphothreonineCombined sources1
Modified residuei405PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ16186.
MaxQBiQ16186.
PaxDbiQ16186.
PeptideAtlasiQ16186.
PRIDEiQ16186.

PTM databases

iPTMnetiQ16186.
PhosphoSitePlusiQ16186.

Expressioni

Gene expression databases

BgeeiENSG00000130706.
CleanExiHS_ADRM1.
ExpressionAtlasiQ16186. baseline and differential.
GenevisibleiQ16186. HS.

Organism-specific databases

HPAiHPA042266.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP) (PubMed:16990800). Interacts with the proteasomal scaffolding protein PSMD1 (PubMed:16990800,PubMed:16815440, PubMed:16906146, PubMed:20471946). Interacts with deubiquitinase UCHL5; this interaction activates the auto-inhibited UCHL5 by deoligomerizing it (PubMed:17139257, PubMed:24752541, PubMed:25702870, PubMed:25702872). Interacts with UBQLN2 and ubiquitin (PubMed:27396824).9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protease binding Source: UniProtKB
  • proteasome binding Source: UniProtKB
  • ubiquitin binding Source: GO_Central

Protein-protein interaction databases

BioGridi116235. 77 interactors.
DIPiDIP-42668N.
IntActiQ16186. 58 interactors.
MINTiMINT-2869171.
STRINGi9606.ENSP00000253003.

Structurei

Secondary structure

1407
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 34Combined sources12
Beta strandi37 – 40Combined sources4
Beta strandi45 – 51Combined sources7
Beta strandi57 – 63Combined sources7
Turni64 – 66Combined sources3
Beta strandi69 – 75Combined sources7
Beta strandi79 – 84Combined sources6
Beta strandi89 – 91Combined sources3
Beta strandi93 – 98Combined sources6
Turni99 – 101Combined sources3
Beta strandi104 – 109Combined sources6
Beta strandi111 – 114Combined sources4
Helixi117 – 129Combined sources13
Turni191 – 195Combined sources5
Beta strandi202 – 204Combined sources3
Beta strandi249 – 253Combined sources5
Turni254 – 256Combined sources3
Helixi266 – 273Combined sources8
Beta strandi276 – 278Combined sources3
Beta strandi282 – 284Combined sources3
Helixi288 – 291Combined sources4
Helixi294 – 297Combined sources4
Helixi300 – 302Combined sources3
Helixi304 – 310Combined sources7
Helixi311 – 313Combined sources3
Helixi324 – 326Combined sources3
Helixi327 – 332Combined sources6
Helixi334 – 348Combined sources15
Turni349 – 351Combined sources3
Helixi353 – 358Combined sources6
Helixi363 – 371Combined sources9
Helixi374 – 383Combined sources10
Beta strandi386 – 388Combined sources3
Beta strandi395 – 397Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KQZNMR-A253-407[»]
2KR0NMR-A1-407[»]
2L5VNMR-A260-407[»]
2MKZNMR-A270-407[»]
2NBKNMR-A20-130[»]
2NBVNMR-A1-150[»]
4UELX-ray2.30C266-388[»]
4UEMX-ray2.82B266-388[»]
4WLQX-ray2.85B286-384[»]
4WLRX-ray2.00B285-386[»]
5IRSX-ray1.80A2-150[»]
5V1YX-ray1.42A/B19-132[»]
5V1ZX-ray2.00A/B19-132[»]
DisProtiDP00839.
ProteinModelPortaliQ16186.
SMRiQ16186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ16186.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 130Pru (pleckstrin-like receptor for ubiquitin) domain1 PublicationAdd BLAST109
Regioni253 – 407Interaction with UCHL51 PublicationAdd BLAST155

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi135 – 202Gly-richAdd BLAST68
Compositional biasi193 – 257Ser-richAdd BLAST65
Compositional biasi203 – 213Poly-SerAdd BLAST11

Domaini

The Pru (pleckstrin-like receptor for ubiquitin) domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.1 Publication

Sequence similaritiesi

Belongs to the ADRM1 family.Curated

Phylogenomic databases

eggNOGiKOG3037. Eukaryota.
ENOG410XSJJ. LUCA.
GeneTreeiENSGT00390000013839.
HOGENOMiHOG000005947.
HOVERGENiHBG073518.
InParanoidiQ16186.
KOiK06691.
OMAiGKMVHPD.
OrthoDBiEOG091G0FEO.
PhylomeDBiQ16186.
TreeFamiTF313410.

Family and domain databases

InterProiView protein in InterPro
IPR006773. 26S_Psome_Ubiquitin-recp_Rpn13.
IPR032368. RPN13_C.
PANTHERiPTHR12225. PTHR12225. 1 hit.
PfamiView protein in Pfam
PF04683. Proteasom_Rpn13. 1 hit.
PF16550. RPN13_C. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q16186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ
60 70 80 90 100
QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA
110 120 130 140 150
GSKRLFFWMQ EPKTDQDEEH CRKVNEYLNN PPMPGALGAS GSSGHELSAL
160 170 180 190 200
GGEGGLQSLL GNMSHSQLMQ LIGPAGLGGL GGLGALTGPG LASLLGSSGP
210 220 230 240 250
PGSSSSSSSR SQSAAVTPSS TTSSTRATPA PSAPAAASAT SPSPAPSSGN
260 270 280 290 300
GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLAS VLTPEIMAPI
310 320 330 340 350
LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASG
360 370 380 390 400
QLGPLMCQFG LPAEAVEAAN KGDVEAFAKA MQNNAKPEQK EGDTKDKKDE

EEDMSLD
Length:407
Mass (Da):42,153
Last modified:January 23, 2002 - v2
Checksum:i2D38811DCA231864
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142S → T in BAA11023 (PubMed:8033103).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64154 mRNA. Translation: BAA11023.1.
AL354836 Genomic DNA. No translation available.
BR000321 mRNA. Translation: FAA00246.1.
BC010733 mRNA. Translation: AAH10733.1.
BC017245 mRNA. Translation: AAH17245.1.
CCDSiCCDS13496.1.
PIRiI52703.
RefSeqiNP_001268366.1. NM_001281437.1.
NP_001268367.1. NM_001281438.1.
NP_008933.2. NM_007002.3.
NP_783163.1. NM_175573.2.
XP_005260314.1. XM_005260257.1.
UniGeneiHs.90107.

Genome annotation databases

EnsembliENST00000253003; ENSP00000253003; ENSG00000130706.
ENST00000491935; ENSP00000478877; ENSG00000130706.
GeneIDi11047.
KEGGihsa:11047.
UCSCiuc002ycn.5. human.

Similar proteinsi

Entry informationi

Entry nameiADRM1_HUMAN
AccessioniPrimary (citable) accession number: Q16186
Secondary accession number(s): A0PKB1, Q96FJ7, Q9H1P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: November 22, 2017
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although initially described as a cell membrane glycoprotein, ADRM1 is intracellular and non-glycosylated, and has probably no direct role in cell adhesion.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families