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Q160T4 (SYH_ROSDO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Histidine--tRNA ligase
EC=6.1.1.21
Alternative name(s):
Histidyl-tRNA synthetase
Short name=HisRS
Gene names
Name:hisS
Ordered Locus Names:RD1_4065
OrganismRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]
Taxonomic identifier375451 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter ›

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP-Rule MF_00127

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processhistidyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

histidine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 519519Histidine--tRNA ligase HAMAP-Rule MF_00127
PRO_1000016438

Sequences

Sequence LengthMass (Da)Tools
Q160T4 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 7132937E94BB3EA8

FASTA51957,028
        10         20         30         40         50         60 
MAKPKKTPRP KAQTPKGFRD YFGAEVSQRA EMLQRIAGVY HRYGFDALES SGVETVEALG 

        70         80         90        100        110        120 
KFLPDVDRPN EGVFAWQEDA DADKPGDWLA LRYDLTAPLA RVYAQHQNDL PKPYRRYAMG 

       130        140        150        160        170        180 
PVWRNEKPGP GRFRQFYQCD ADTVGASSVA ADAEICAMLC DCLEAVGIER GDYVVRINNR 

       190        200        210        220        230        240 
KVLNGVLEVI GALGDEPRLS QHFQEMAENL GKKMTAAKVG IVLRTIDKLD RLGKEGVRSL 

       250        260        270        280        290        300 
LGPGRKDESG DLTFGADLSK AQIDVVMSFL EAKGGTNSET LINLREIVGR SRIGKEGVDE 

       310        320        330        340        350        360 
LEKISELLSA GSYGPDRIVI DPSVVRGLGY YTGPVYEAEL TFEIQDDKGR TRNFGSVAGG 

       370        380        390        400        410        420 
GRYDDLVKRF TGQEVPATGV SIGVDRLLAA LHAKGRLDTT DVGPVVVTVM DRDRMADYQA 

       430        440        450        460        470        480 
MVAELRQAGI RAEVYLGNPK NFGNQLKYAD TRNSPVAVIE GGDEHEKGVV QIKDLILGAE 

       490        500        510 
IAKSATLEEW KDRPSQFEVP RAELVAKVRE ILAQHGLEK

« Hide

References

[1]"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism."
Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W.
J. Bacteriol. 189:683-690(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33942 / OCh 114.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000362 Genomic DNA. Translation: ABG33509.1.
RefSeqYP_684195.1. NC_008209.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING375451.RD1_4065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG33509; ABG33509; RD1_4065.
GeneID4195330.
KEGGrde:RD1_4065.
PATRIC23366043. VBIRosDen86677_3886.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0124.
HOGENOMHOG000018071.
KOK01892.
OMAENTQKGR.
ProtClustDBPRK00037.

Enzyme and pathway databases

BioCycRDEN375451:GJIZ-3827-MONOMER.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
HAMAPMF_00127. His_tRNA_synth.
InterProIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
[Graphical view]
PANTHERPTHR11476. PTHR11476. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
TIGRFAMsTIGR00442. hisS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYH_ROSDO
AccessionPrimary (citable) accession number: Q160T4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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