Reviewed, UniProtKB/Swiss-Prot Q160C1 (FOLD2_ROSDO)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folD 2
Including the following 2 domains:
    1- Recommended name:
            Methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.5
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	RD1_4236

Organism

Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans) [Complete proteome] [HAMAP]

Taxonomic identifier

375451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Roseobacter

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate pathway. HAMAP MF_01576
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Bifunctional protein folD 2 HAMAP MF_01576		PRO_0000268483

Sequences

	Sequence		Length	Mass (Da)
	Q160C1-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 3FE1088477B13EA0	FASTA	300	31,422
	10 20 30 40 50 60 MAATIIDGKA FAAKVRAQVA EHVTRIKQDH GITPGLAVVL VGEDPASQVY VRSKGKQTVE 70 80 90 100 110 120 AGMNSYEHKL DADTSEADLL AVVDKLNKDP NVHGILVQLP LPKHLDEDLI INSIDPAKDV 130 140 150 160 170 180 DGFHISNVGL LGTGQKSMVP CTPLGCLMML REHHGSLSGM NAVVIGRSNI VGKPMAQLLL 190 200 210 220 230 240 NDSCTVTIAH SRTKDLPDVV RGADIVVAAV GRPEMVPGDW IKPGATVIDV GINRIDAPEK 250 260 270 280 290 300 GEGKTRLVGD CHYDSCAETA GAITPVPGGV GPMTIACLLA NTVTSCCRAN GLEEPTGLTA

References

[1]

"The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism." Swingley W.D., Sadekar S., Mastrian S.D., Matthies H.J., Hao J., Ramos H., Acharya C.R., Conrad A.L., Taylor H.L., Dejesa L.C., Shah M.K., O'Huallachain M.E., Lince M.T., Blankenship R.E., Beatty J.T., Touchman J.W. J. Bacteriol. 189:683-690(2007) [PubMed: 17098896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000362 Genomic DNA. Translation: ABG33672.1.
RefSeq	YP_684358.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4195554.
GenomeReviews	Gene locus RD1_4236 in contig CP000362_GR.
KEGG	rde:RD1_4236.
NMPDR	fig|375451.6.peg.3934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q160C1.
OMA	Q160C1. MQIIDGK.
Enzyme and pathway databases
BioCyc	RDEN375451:RD1_4236-MON.
Family and domain databases
HAMAP	MF_01576. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_ROSDO

Accession

Primary (citable) accession number: Q160C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 25, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)