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Q15ZS8 (TRMB_PSEA6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Patl_0078
OrganismPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) [Complete proteome] [HAMAP]
Taxonomic identifier342610 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288202

Regions

Region217 – 2204Substrate binding By similarity

Sites

Active site1441 By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site941S-adenosyl-L-methionine By similarity
Binding site1211S-adenosyl-L-methionine By similarity
Binding site1441S-adenosyl-L-methionine By similarity
Binding site1481Substrate By similarity
Binding site1801Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q15ZS8 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 3865642D6AF7F1C2

FASTA23927,136
        10         20         30         40         50         60 
MSQFKSKEEA AESGVYIRKI QSFVKREGRL TKGQEKAIES NWETMGLEHK SGLLDMNEVF 

        70         80         90        100        110        120 
GRTAPVVLEI GFGMGKSLVT MAKNEPDKDF IGIEVHRPGI GACLADAQEQ GVTNLRVYEH 

       130        140        150        160        170        180 
DAVEVLADCI ADGSLSRMQL FFPDPWHKKR HHKRRIVQPE FVEKLRGKLA IGGVFHMATD 

       190        200        210        220        230 
WENYAEHMLE IMAVAPGYKN LSSTSDYVPR PEQRPLTKFE QRGHRLGHGV WDLMFERVA 

« Hide

References

[1]"Complete sequence of Pseudoalteromonas atlantica T6c."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T6c / ATCC BAA-1087.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000388 Genomic DNA. Translation: ABG38610.1.
RefSeqYP_659664.1. NC_008228.1.

3D structure databases

ProteinModelPortalQ15ZS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342610.Patl_0078.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG38610; ABG38610; Patl_0078.
GeneID4171779.
KEGGpat:Patl_0078.
PATRIC23044940. VBIPseAtl25434_0081.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycPATL342610:GHGT-84-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PSEA6
AccessionPrimary (citable) accession number: Q15ZS8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways