Reviewed, UniProtKB/Swiss-Prot Q15XV2 (ACEK_PSEA6)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Patl_0758

Organism

Pseudoalteromonas atlantica (strain T6c / BAA-1087) [Complete proteome] [HAMAP]

Taxonomic identifier

342610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288292
Regions
	Nucleotide binding	324 – 330	7	ATP By similarity
Sites
	Active site	380	1	By similarity
	Binding site	345	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15XV2-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 8245846F42EE0066	FASTA	577	67,321
	10 20 30 40 50 60 MNNTHSSVLN KVAYLILHGF DKSYRWHSRI TRDAQQRFEQ ALWQETQKAV KERIAIYERT 70 80 90 100 110 120 LADAVGEIYQ QVFPHQENNQ FWFDLKTRYQ KILSDHPQYE LAETFYNSVL GRIFKHQKIN 130 140 150 160 170 180 DEMMFIMPTR CYLAGLQRHL VVHSFDTSGT VRRMLEDIFS QYHFDIAFQD MQRDLQHLDG 190 200 210 220 230 240 ALRARLNREQ LASVHTVEML KPVFYRSKSA YLIGRICMPD ETLPFVIPLS IAEAETSGEK 250 260 270 280 290 300 HKIVVEALLT ERQDLSVVFS FARAYFMADT QHPAEVVAFL HELLPHKKKF ELYIALGLYK 310 320 330 340 350 360 HGKTVFYRNF LAHLEESNDQ FSIAPGIRGL VMAVFHLPSY GVVFKIIKDE FPESKKITRQ 370 380 390 400 410 420 HVKDCYKLVK MTDRVGRMAD THEYVNFRLP RHRVEQALVD ELLDCCASSI ELTDEEVIIK 430 440 450 460 470 480 HLYIERKMTP LNIFLEQQPD PALITSALND LGLCIKQIAA AHIFAGDMLH KNFGITRGGR 490 500 510 520 530 540 VIFYDYDEIC YLTEREFRTL PKSDDPYAID TLSVGPTDVF PEQFEHFIVG KKHLKQELKA 550 560 570 LHGEIMTAEY WQHMQAQSLK GDVPDFIPYN QAKRFVN

References

[1]

"Complete sequence of Pseudoalteromonas atlantica T6c." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000388 Genomic DNA. Translation: ABG39286.1.
RefSeq	YP_660340.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q15XV2.
Genome annotation databases
GeneID	4173936.
GenomeReviews	Gene locus Patl_0758 in contig CP000388_GR.
KEGG	pat:Patl_0758.
NMPDR	fig|342610.3.peg.3031.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q15XV2.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	PATL342610:PATL_0758-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEA6

Accession

Primary (citable) accession number: Q15XV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)