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Q15SR5 (BIOB_PSEA6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Patl_2557
OrganismPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) [Complete proteome] [HAMAP]
Taxonomic identifier342610 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Biotin synthase HAMAP-Rule MF_01694
PRO_0000381551

Sites

Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1411Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2011Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2731Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q15SR5 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: 5A1132038ABBC37D

FASTA36941,341
        10         20         30         40         50         60 
MSVNVDATSS SGASEVRHDW TLAEVNALFA MPFNDLLFKA QCVHRAHFDP NYVQVSTLLS 

        70         80         90        100        110        120 
IKTGACPEDC KYCPQSARYD TGLEKERLME IEKVIQRARE AKAAGSTRFC MGAAWRNPKE 

       130        140        150        160        170        180 
RDMPYVTDMV REVKKLGLET CMTLGMLTRE QAIALQQAGL DYYNHNLDTS PEFYGDIITT 

       190        200        210        220        230        240 
RTYQDRLNTL DAVREAGMNV CSGGIVGMGE TGSDRSGLLM QLANLEHQPE SVPINMLVKV 

       250        260        270        280        290        300 
KGTPMENVED LDYFEFIRTI AVARIMMPKS FVRLSAGREA MNEQMQAMCF MAGANSIFYG 

       310        320        330        340        350        360 
CKLLTTSNPE THEDVMLFKK LGINAKQTRE YSDEAHQAAL LDEIQTNEAP QAPEMFYDAT 


QKKPETVNI 

« Hide

References

[1]"Complete sequence of Pseudoalteromonas atlantica T6c."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T6c / ATCC BAA-1087.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000388 Genomic DNA. Translation: ABG41073.1.
RefSeqYP_662127.1. NC_008228.1.

3D structure databases

ProteinModelPortalQ15SR5.
SMRQ15SR5. Positions 17-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342610.Patl_2557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG41073; ABG41073; Patl_2557.
GeneID4174312.
KEGGpat:Patl_2557.
PATRIC23050310. VBIPseAtl25434_2732.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPATL342610:GHGT-2604-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PSEA6
AccessionPrimary (citable) accession number: Q15SR5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 25, 2006
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways