Reviewed, UniProtKB/Swiss-Prot Q15R69 (PUR4_PSEA6)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	Patl_3113

Organism

Pseudoalteromonas atlantica (strain T6c / BAA-1087) [Complete proteome] [HAMAP]

Taxonomic identifier

342610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264585
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	306 – 317	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15R69-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: ADA7B6143201C924	FASTA	1,295	140,706
	10 20 30 40 50 60 MLVLRGAPAL SEFRTKKLMQ SIEALNLPVH ALFAEFVHFA QVSAQLEAND QQVLDKLLTY 70 80 90 100 110 120 GPKIQEKQHK GLLLLVIPRA GTISPWSSKA TDIAHNCGLN NVERLERGCA FYIDADPLND 130 140 150 160 170 180 TQLIQLKNVL HDRMTQSVVS DLQDASILFK HEAPKPLTSI DVLGGGREEL VSANVRLGLA 190 200 210 220 230 240 LAEDEVDYLV SSFEKLGRNP NDIELYMFAQ ANSEHCRHKI FNADWTIDGQ TQPKSLFKMI 250 260 270 280 290 300 KNTYEKCPDY VHSAYADNAA VMEGSFAGRF FPQADNNQYR YHHEDIDILM KVETHNHPTA 310 320 330 340 350 360 IAPFSGAATG SGGEIRDEGA TGRGSKPKAG LVGFSVSNLR IPGFEQPWEN DYGKPERIVT 370 380 390 400 410 420 AFDIMLNGPL GGAAFNNEFG RPNILGYFRT YEQKVTSFNG EEVRGYHKPI MLAGGLGNIR 430 440 450 460 470 480 KQHTQKGEIT VGAKLIALGG PAMNIGLGGG AASSMASGES NEDLDFASVQ RDNPEMERRC 490 500 510 520 530 540 QEVIDKCWQL GENNPIQFIH DVGAGGLSNA FPELVNDGGR GAVFSLRNVP NDEPGMTPLE 550 560 570 580 590 600 VWCNESQERY VMSVAPENLA TFTDICARER APFAVVGEAT QARHLTLDDS HFNNKPIDMP 610 620 630 640 650 660 LDVLLGKAPK MHRDVQSKAL TGNAFDVSNI DLDEAALRLL HLPAVAEKTF LITIGDRSVT 670 680 690 700 710 720 GLVNRDQMVG PWQIPVADVA VTAAAFDTYQ GEAMSLGERT PAALLNYGAS ARLAVGEALT 730 740 750 760 770 780 NIAAADIGDL KRIKLSANWM AAAGHPGEDA GLYEAVKAVG EELCPALGLT IPVGKDSMSM 790 800 810 820 830 840 KTQWQDAQGD KAVTSPMSLV ITAFGAVKDV RKTLTPELTT KGDTCLMLID LGAGQNRMGA 850 860 870 880 890 900 SCLAQVYQQL GDKTPDVDSP ELLKGFFLAI QQLVNEKALH AYHDRSDGGL FTTLVEMAFA 910 920 930 940 950 960 GNTGLDIKLD ALTGDNASAL FNEELGAVIQ FDADKLNQVD AILAANGLSG VSHVIGTLSD 970 980 990 1000 1010 1020 DDQIRFSREG EPVLQNSRGV YRNAWAQTTH HMQRLRDNPE CAEQELASKN DLNNPGLHAA 1030 1040 1050 1060 1070 1080 LSFDVTEDVA APYIAKGIAP KMAILREQGV NSHVEMAAAF DRAGFASVDV HMSDILAGRV 1090 1100 1110 1120 1130 1140 SLSEFQGLVA CGGFSYGDVL GAGEGWAKSI LFNAMARDEF SAFFERNETF SLGVCNGCQM 1150 1160 1170 1180 1190 1200 LSNLKSLIPG AEHWPHFVTN QSERFEARVA MLEVKDSPSI FFKGMQGSKM PIAVSHGEGR 1210 1220 1230 1240 1250 1260 AEFAQTTGLE AALDSNTIAL QYVDNYGKVT EQYPANPNGS PAGISGLTSK DGRATIMMPH 1270 1280 1290 PERVFRTVAN SWHPDDWQED SPWMRMFRNA RVYLG

References

[1]

"Complete sequence of Pseudoalteromonas atlantica T6c." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000388 Genomic DNA. Translation: ABG41619.1.
RefSeq	YP_662673.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q15R69.
Genome annotation databases
GeneID	4174886.
GenomeReviews	Gene locus Patl_3113 in contig CP000388_GR.
KEGG	pat:Patl_3113.
NMPDR	fig|342610.3.peg.3939.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q15R69.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	PATL342610:PATL_3113-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_PSEA6

Accession

Primary (citable) accession number: Q15R69

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 25, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)