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Q15R04

- SYI_PSEA6

UniProt

Q15R04 - SYI_PSEA6

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei566 – 5661Aminoacyl-adenylateUniRule annotation
Binding sitei610 – 6101ATPUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPATL342610:GHGT-3247-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Patl_3178
OrganismiPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Taxonomic identifieri342610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000001981: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Isoleucine--tRNA ligasePRO_1000022102Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi342610.Patl_3178.

Structurei

3D structure databases

ProteinModelPortaliQ15R04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi607 – 6115"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15R04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETEFPMRGN LAQREPKMLQ DWTNKALYHK IRAAKKGKTP
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDVIVK SKTLSDFDSP YVPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGKKVTAA EFRQKCRDYA EKQINGQKAD FIRLGVLGEW
160 170 180 190 200
DKPYRTMDFS SEANIIRELG NVVRSGHLEK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYQDKVSPSI DVRFNIADEA SFTKQFTAIG EGLGEGTISV VIWTTTPWTL
260 270 280 290 300
PANRAVSLHP ELEYSLVQVQ SENGNERLVV ATDLLDECVK RFGFENTHVL
310 320 330 340 350
GTALGQALEN MQVQHPFYDF TVPLILGEHV TTDSGTGCVH TAPGHGVEDF
360 370 380 390 400
NVGRQYNLEV ANPVGANGVY LENTPIFAGE HVFKANEHVV EVLAERGALV
410 420 430 440 450
HHVKYTHSYP HCWRHKTPLI FRATPQWFIS MDKNGLRAAS IKEIHKTQWI
460 470 480 490 500
PEWGESRIES MVAGRPDWCI SRQRTWGVPI ALFVHKDTGD LHPNTDALIE
510 520 530 540 550
QVAQRVESEG IQAWWDIDPQ EMLGDDADTF VKVLDTLDVW FDSGVSHYFV
560 570 580 590 600
VDKRDDIPAS ADLYLEGSDQ HRGWFMSSLM TSVATKGHAP YKQVLTHGFT
610 620 630 640 650
VDGKGKKMSK SLGNTVAPQQ VTNKLGADIL RLWVASTDYR SEIAVSDEIL
660 670 680 690 700
KRSADAYRRI RNTSRFLLAN LTGFNPKTDL VPFEEMVELD KWAVSRAHTM
710 720 730 740 750
QREIVKAYEA YDLLVVTQKL MQFCSIEMGS FYLDIIKDRQ YTAKSDSHAR
760 770 780 790 800
RSCQSALYHI VEALVRWMAP ITSFTAQEIW QEMPWQEDEF VFTGTWYTGL
810 820 830 840 850
TAQADNTEFN DAFWQQVLAV KDEVNRTIEL ARKEGTIGGS LEAEVKIYAT
860 870 880 890 900
AELAELLSKL QDEARFVFIT SSATVEAVES KPADATETEV PGLWLHISAS
910 920 930 940
EGKKCARCWH HREDVGTNES HPELCQRCVT NVDGEGETRA YA
Length:942
Mass (Da):106,094
Last modified:July 25, 2006 - v1
Checksum:iE8CDF6611BE0E24F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000388 Genomic DNA. Translation: ABG41684.1.
RefSeqiWP_011575915.1. NC_008228.1.
YP_662738.1. NC_008228.1.

Genome annotation databases

EnsemblBacteriaiABG41684; ABG41684; Patl_3178.
GeneIDi4174955.
KEGGipat:Patl_3178.
PATRICi23051666. VBIPseAtl25434_3386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000388 Genomic DNA. Translation: ABG41684.1 .
RefSeqi WP_011575915.1. NC_008228.1.
YP_662738.1. NC_008228.1.

3D structure databases

ProteinModelPortali Q15R04.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342610.Patl_3178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG41684 ; ABG41684 ; Patl_3178 .
GeneIDi 4174955.
KEGGi pat:Patl_3178.
PATRICi 23051666. VBIPseAtl25434_3386.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PATL342610:GHGT-3247-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T6c / ATCC BAA-1087.

Entry informationi

Entry nameiSYI_PSEA6
AccessioniPrimary (citable) accession number: Q15R04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3