Reviewed, UniProtKB/Swiss-Prot Q15QK3 (E4PD_PSEA6)

Names and origin

Protein names

Recommended name:
    D-erythrose-4-phosphate dehydrogenase
      Short name=E4PDH
    EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	Patl_3329

Organism

Pseudoalteromonas atlantica (strain T6c / BAA-1087) [Complete proteome] [HAMAP]

Taxonomic identifier

342610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity.
Catalytic activity	D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	D-erythrose-4-phosphate dehydrogenase		PRO_0000293153
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	153 – 155	3	Substrate binding Potential
	Region	212 – 213	2	Substrate binding Potential
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	199	1	Substrate Potential
	Binding site	235	1	Substrate Potential
	Binding site	317	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15QK3-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: E5283885B5CC349A	FASTA	342	37,500
	10 20 30 40 50 60 MIRVAINGFG RVGRNVLRAL YETGQNKQIQ IVAINELAEP EGVAHLLKYD TAHGRFRFPV 70 80 90 100 110 120 VLDNQQLVVA GDHITLLQQE NIRDLPWAQL DIDVVLECTG VNNERVHGEQ HIAQGAKKVL 130 140 150 160 170 180 FSQPCGPDVD ATIIMGINEE SLKASDSIVS AGSCTTNCIV PVIQVLDEAF SVSSGTITTI 190 200 210 220 230 240 HSSMHDQQVI DAYHPDLRRT RAASQSIIPV DTKLARGIER VLPKFAGKFE AIAVRVPTIN 250 260 270 280 290 300 VSAMDLSVTL DAKVTIDDVN QALKTVKNGR LNGILDFTEE PLVSVDFNHD AHSCIVDGTQ 310 320 330 340 TRVSHKQLVK LLVWCDNEWG FSNRMIDIVL VMNTLSLGNT GK

References

[1]

"Complete sequence of Pseudoalteromonas atlantica T6c." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000388 Genomic DNA. Translation: ABG41835.1.
RefSeq	YP_662889.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4175106.
GenomeReviews	Gene locus Patl_3329 in contig CP000388_GR.
KEGG	pat:Patl_3329.
NMPDR	fig|342610.3.peg.1260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q15QK3.
Enzyme and pathway databases
BioCyc	PATL342610:PATL_3329-MON.
Family and domain databases
HAMAP	MF_01640. [Tree]
InterPro	IPR006422. E4P_DHase_bac. IPR000173. GlycerAld_3-P_DHase. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_PSEA6

Accession

Primary (citable) accession number: Q15QK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)