Reviewed, UniProtKB/Swiss-Prot Q15NK1 (CYSI_PSEA6)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Patl_4038

Organism

Pseudoalteromonas atlantica (strain T6c / BAA-1087) [Complete proteome] [HAMAP]

Taxonomic identifier

342610 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Pseudoalteromonadaceae › Pseudoalteromonas

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000292961
Sites
	Metal binding	433	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	439	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	482	1	Iron (siroheme axial ligand) By similarity
	Metal binding	482	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15NK1-1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 46A2EFB0690E54C4	FASTA	569	63,144
	10 20 30 40 50 60 MTTDNKFVVE GKLADNERLK GESNFLRGTI AEDLKDDLTG AFVGDNFQLI RFHGMYQQDD 70 80 90 100 110 120 RDLRAERAKQ KLEPLQNVML RARLPGGIIK PQQWLAIDKF AEEKSLYGSI RLTTRQTFQF 130 140 150 160 170 180 HGVLKPNIKL MHQTLNQVGI DSIATAGDVN RNVLCTSNPI ESAVHQEAYE WATKISEHLL 190 200 210 220 230 240 PKTRAYAEIW LDGEKKETTD HEPILGANYL PRKFKTTVAI PPLNDVDVHA NDLNFIAISK 250 260 270 280 290 300 DGKLVGFNVL VGGGLAMTHG DHATFPRKAS DFGFIKVEDT LAIAEAVVST QRDWGNRVNR 310 320 330 340 350 360 KNAKTKYTLE RVGVENFKAE VEKRSGVTFG ESQAYEFTER GDRIGWVEGI DGKHHLTLFI 370 380 390 400 410 420 ENGRILDYPG KPLKTGCAEI AKIHDGDFRL TANQNLIVAG VSEQNKAKVE EIARAHGLIE 430 440 450 460 470 480 DDLSAQRKDS MACVALPTCP LAMAEAERYL PEAVTQLEGI LAKHAIAQKS IIYRVTGCPN 490 500 510 520 530 540 GCGRSMLAEI GLVGKGPGKY NLHLGGNRQG TRIPKMYKEN IGEQQIMDEL DVLIGQWAKE 550 560 AQSDESFGDF VIRTGVIAEV VNSAEDFYA

References

[1]

"Complete sequence of Pseudoalteromonas atlantica T6c." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000388 Genomic DNA. Translation: ABG42537.1.
RefSeq	YP_663591.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4175827.
GenomeReviews	Gene locus Patl_4038 in contig CP000388_GR.
KEGG	pat:Patl_4038.
NMPDR	fig|342610.3.peg.2295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q15NK1.
Enzyme and pathway databases
BioCyc	PATL342610:PATL_4038-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_PSEA6

Accession

Primary (citable) accession number: Q15NK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	July 25, 2006
Last modified:	November 25, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)