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Q15N09 (BIOH_PSEA6) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esterase

EC=3.1.1.85
Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene names
Name:bioH
Ordered Locus Names:Patl_4230
OrganismPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) [Complete proteome] [HAMAP]
Taxonomic identifier342610 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity. HAMAP-Rule MF_01260

Catalytic activity

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol. HAMAP-Rule MF_01260

Pathway

Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01260

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01260

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01260.

Sequence similarities

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   Molecular functionHydrolase
Serine esterase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarboxylic ester hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258Pimeloyl-[acyl-carrier protein] methyl ester esterase HAMAP-Rule MF_01260
PRO_1000085809

Regions

Region84 – 852Substrate binding By similarity
Region145 – 1495Substrate binding By similarity

Sites

Active site841Nucleophile By similarity
Active site2091 By similarity
Active site2381 By similarity
Binding site221Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2381Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q15N09 [UniParc].

Last modified July 25, 2006. Version 1.
Checksum: CCBFA701ECAA6DEF

FASTA25828,421
        10         20         30         40         50         60 
MHTTLKTRTV GSGPNFVLLH GWGVNSGVWQ PIAKQLEQHF SVTYVDLPGF GENNQIMPKP 

        70         80         90        100        110        120 
YDLKNLAECV ANVLPENSVL AGWSLGGLVA QHVALLEPTN VKQLILIATS PKFQKGNDWA 

       130        140        150        160        170        180 
GIDPNILQAF SQQLVKNLSK TIERFLAIQA MGSDSAKTDI RKIKNSIEAS PQADIAALTA 

       190        200        210        220        230        240 
GLDILEHVDL RDQIAALKMP IHWMLGRLDS LVPVKLQGYV QRSLAKNHSV TIFPHASHAP 

       250 
FISHTEDFLI DLMENTLR 

« Hide

References

[1]"Complete sequence of Pseudoalteromonas atlantica T6c."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C., Bartlett D., Higgins B.P., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T6c / ATCC BAA-1087.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000388 Genomic DNA. Translation: ABG42729.1.
RefSeqYP_663783.1. NC_008228.1.

3D structure databases

ProteinModelPortalQ15N09.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING342610.Patl_4230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG42729; ABG42729; Patl_4230.
GeneID4176020.
KEGGpat:Patl_4230.
PATRIC23053920. VBIPseAtl25434_4500.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000028062.
KOK02170.
OMAFDKAAHA.
OrthoDBEOG6FJNH9.

Enzyme and pathway databases

BioCycPATL342610:GHGT-4312-MONOMER.
UniPathwayUPA00078.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
HAMAPMF_01260. Carboxylester.
InterProIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PRINTSPR00111. ABHYDROLASE.
SUPFAMSSF53474. SSF53474. 1 hit.
TIGRFAMsTIGR01738. bioH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOH_PSEA6
AccessionPrimary (citable) accession number: Q15N09
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways