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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei84 – 841NucleophileUniRule annotation
Active sitei209 – 2091UniRule annotation
Active sitei238 – 2381UniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciPATL342610:GHGT-4312-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Patl_4230
OrganismiPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Taxonomic identifieri342610 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
ProteomesiUP000001981: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000085809Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi342610.Patl_4230.

Structurei

3D structure databases

ProteinModelPortaliQ15N09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 852Substrate bindingUniRule annotation
Regioni145 – 1495Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiFDKAAHA.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q15N09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTTLKTRTV GSGPNFVLLH GWGVNSGVWQ PIAKQLEQHF SVTYVDLPGF
60 70 80 90 100
GENNQIMPKP YDLKNLAECV ANVLPENSVL AGWSLGGLVA QHVALLEPTN
110 120 130 140 150
VKQLILIATS PKFQKGNDWA GIDPNILQAF SQQLVKNLSK TIERFLAIQA
160 170 180 190 200
MGSDSAKTDI RKIKNSIEAS PQADIAALTA GLDILEHVDL RDQIAALKMP
210 220 230 240 250
IHWMLGRLDS LVPVKLQGYV QRSLAKNHSV TIFPHASHAP FISHTEDFLI

DLMENTLR
Length:258
Mass (Da):28,421
Last modified:July 25, 2006 - v1
Checksum:iCCBFA701ECAA6DEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000388 Genomic DNA. Translation: ABG42729.1.
RefSeqiYP_663783.1. NC_008228.1.

Genome annotation databases

EnsemblBacteriaiABG42729; ABG42729; Patl_4230.
GeneIDi4176020.
KEGGipat:Patl_4230.
PATRICi23053920. VBIPseAtl25434_4500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000388 Genomic DNA. Translation: ABG42729.1.
RefSeqiYP_663783.1. NC_008228.1.

3D structure databases

ProteinModelPortaliQ15N09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342610.Patl_4230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG42729; ABG42729; Patl_4230.
GeneIDi4176020.
KEGGipat:Patl_4230.
PATRICi23053920. VBIPseAtl25434_4500.

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiFDKAAHA.
OrthoDBiEOG6FJNH9.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciPATL342610:GHGT-4312-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T6c / ATCC BAA-1087.

Entry informationi

Entry nameiBIOH_PSEA6
AccessioniPrimary (citable) accession number: Q15N09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 25, 2006
Last modified: March 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.