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Q15N09

- BIOH_PSEA6

UniProt

Q15N09 - BIOH_PSEA6

Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

    Catalytic activityi

    Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Active sitei84 – 841NucleophileUniRule annotation
    Active sitei209 – 2091UniRule annotation
    Active sitei238 – 2381UniRule annotation
    Binding sitei238 – 2381SubstrateUniRule annotation

    GO - Molecular functioni

    1. carboxylic ester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Biotin biosynthesis

    Enzyme and pathway databases

    BioCyciPATL342610:GHGT-4312-MONOMER.
    UniPathwayiUPA00078.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
    Alternative name(s):
    Biotin synthesis protein BioHUniRule annotation
    Carboxylesterase BioHUniRule annotation
    Gene namesi
    Name:bioHUniRule annotation
    Ordered Locus Names:Patl_4230
    OrganismiPseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)
    Taxonomic identifieri342610 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPseudoalteromonadaceaePseudoalteromonas
    ProteomesiUP000001981: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 258258Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000085809Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi342610.Patl_4230.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15N09.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni84 – 852Substrate bindingUniRule annotation
    Regioni145 – 1495Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000028062.
    KOiK02170.
    OMAiFDKAAHA.
    OrthoDBiEOG6FJNH9.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_01260. Carboxylester.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR010076. BioH.
    [Graphical view]
    PRINTSiPR00111. ABHYDROLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR01738. bioH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q15N09-1 [UniParc]FASTAAdd to Basket

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    MHTTLKTRTV GSGPNFVLLH GWGVNSGVWQ PIAKQLEQHF SVTYVDLPGF    50
    GENNQIMPKP YDLKNLAECV ANVLPENSVL AGWSLGGLVA QHVALLEPTN 100
    VKQLILIATS PKFQKGNDWA GIDPNILQAF SQQLVKNLSK TIERFLAIQA 150
    MGSDSAKTDI RKIKNSIEAS PQADIAALTA GLDILEHVDL RDQIAALKMP 200
    IHWMLGRLDS LVPVKLQGYV QRSLAKNHSV TIFPHASHAP FISHTEDFLI 250
    DLMENTLR 258
    Length:258
    Mass (Da):28,421
    Last modified:July 25, 2006 - v1
    Checksum:iCCBFA701ECAA6DEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000388 Genomic DNA. Translation: ABG42729.1.
    RefSeqiYP_663783.1. NC_008228.1.

    Genome annotation databases

    EnsemblBacteriaiABG42729; ABG42729; Patl_4230.
    GeneIDi4176020.
    KEGGipat:Patl_4230.
    PATRICi23053920. VBIPseAtl25434_4500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000388 Genomic DNA. Translation: ABG42729.1 .
    RefSeqi YP_663783.1. NC_008228.1.

    3D structure databases

    ProteinModelPortali Q15N09.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342610.Patl_4230.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG42729 ; ABG42729 ; Patl_4230 .
    GeneIDi 4176020.
    KEGGi pat:Patl_4230.
    PATRICi 23053920. VBIPseAtl25434_4500.

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000028062.
    KOi K02170.
    OMAi FDKAAHA.
    OrthoDBi EOG6FJNH9.

    Enzyme and pathway databases

    UniPathwayi UPA00078 .
    BioCyci PATL342610:GHGT-4312-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    HAMAPi MF_01260. Carboxylester.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR010076. BioH.
    [Graphical view ]
    PRINTSi PR00111. ABHYDROLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    TIGRFAMsi TIGR01738. bioH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: T6c / ATCC BAA-1087.

    Entry informationi

    Entry nameiBIOH_PSEA6
    AccessioniPrimary (citable) accession number: Q15N09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3