Q15JG1 (Q15JG1_STRHY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Putative glycosyltransferase EMBL ABC67269.1

Gene names

Name:

vldE EMBL ABC67269.1

Organism

Streptomyces hygroscopicus subsp. limoneus EMBL ABC67269.1

Taxonomic identifier

264445 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces hygroscopicus

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Transferase EMBL ABC67269.1
Technical term	3D-structure PDB 3T5T PDB 3T7D PDB 3VDM PDB 3VDN PDB 4F96 PDB 4F97 PDB 4F9F
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q15JG1 [UniParc]. Last modified July 25, 2006. Version 1. Checksum: 91CDAB5D51B859DC	FASTA	497	54,943
	10 20 30 40 50 60 MTGSEIFLAS KRAAITYDTD PATGEPRAWL APGGTGNVVA EQAGVLNISW IASADSEDDR 70 80 90 100 110 120 RASALNPDGV TMELHSGREI LVRLIRHDPA VFRNVQNFMT ANLMWAANNY GWDRWTQPSF 130 140 150 160 170 180 GSDAREGWAD FGRFTRDFAD AILKSSAQSA DPVYLVHDYQ LVGVPALLRE QRPDAPILLF 190 200 210 220 230 240 VHIPWPSADY WRILPKEIRT GILHGMLPAT TIGFFADRWC RNFLESVADL LPDARIDREA 250 260 270 280 290 300 MTVEWRGHRT RLRTMPLGYS PLTLDGRNPQ LPEGIEEWAD GHRLVVHSGR TDPIKNAERA 310 320 330 340 350 360 VRAFVLAARG GGLEKTRMLV RMNPNRLYVP ANADYVHRVE TAVAEANAEL GSDTVRIDND 370 380 390 400 410 420 NDVNHTIACF RRADLLIFNS TVDGQNLSTF EAPLVNERDA DVILSETCGA AEVLGEYCRS 430 440 450 460 470 480 VNPFDLVEQA EAISAALAAG PRQRAEAAAR RRDAARPWTL EAWVQAQLDG LAADHAARTA 490 TAERFDTAPA VSTRADL

References

[1]	"Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from Streptomyces hygroscopicus var. limoneus KCCM 11405 (IFO 12704)." Singh D., Seo M.J., Kwon H.J., Rajkarnikar A., Kim K.R., Kim S.O., Suh J.W. Gene 376:13-23(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: KCCM 11405 EMBL ABC67269.1.
[2]	"Structural basis for the substrate specificity of ValL." Zhang H., Zheng L., Qian H., Chen J. Submitted (JUL-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-497.
[3]	"Structural basis for the substrate specificity of ValL." Zhang H., Zheng L., Qian H. Submitted (JUL-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-497.
[4]	"Mechanistic insights into validoxylamine A 7'-phosphate synthesis by VldE using the structure of the entire product complex." Cavalier M.C., Yim Y.S., Asamizu S., Neau D., Almabruk K.H., Mahmud T., Lee Y.H. PLoS ONE 7:e44934-e44934(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS).
[5]	"Crystal Structure of the VldE, the pseudo-glycosyltransferase, which catalyzes non-glycosidic C-N coupling in Validamycin A biosynthesis." Cavalier M.C., Yim Y.-S., Asamizu S., Neau D., Mahmud T., Lee Y.-H. Submitted (JAN-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
[6]	"Crystal Structure of VldE, the pseudo-glycosyltransferase which catalyzes non-glycosidic C-N coupling in Validamycin A biosynthesis." Cavalier M.C., Yim Y.-S., Asamizu S., Neau D., Mahmud T., Lee Y.-H. Submitted (JAN-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ223652 Genomic DNA. Translation: ABC67269.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3T5T	X-ray	1.70	A/B	2-497	[»]
3T7D	X-ray	1.70	A/B	2-497	[»]
3VDM	X-ray	1.98	A/B	1-497	[»]
3VDN	X-ray	2.55	B	1-497	[»]
4F96	X-ray	2.15	A/B	1-497	[»]
4F97	X-ray	2.11	A/B	1-497	[»]
4F9F	X-ray	2.81	A/B/C/D/E/F	1-497	[»]

ModBase

Search...

Protein family/group databases

CAZy

GT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-13774.

Family and domain databases

InterPro

IPR001830. Glyco_trans_20.
[Graphical view]

Pfam

PF00982. Glyco_transf_20. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q15JG1_STRHY

Accession

Primary (citable) accession number: Q15JG1

Entry history

Integrated into UniProtKB/TrEMBL:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)