Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q15JG1

- Q15JG1_STRHY

UniProt

Q15JG1 - Q15JG1_STRHY

Protein
Submitted name:

Putative glycosyltransferase

Gene

vldE

Organism
Streptomyces hygroscopicus subsp. limoneus
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (25 Jul 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi15 – 151Magnesium 1; via carbonyl oxygen
    Metal bindingi55 – 551Magnesium 1
    Metal bindingi59 – 591Magnesium 1
    Metal bindingi105 – 1051Magnesium 2
    Metal bindingi114 – 1141Magnesium 4Imported
    Metal bindingi187 – 1871Magnesium 3Imported
    Metal bindingi218 – 2181Magnesium 4Imported
    Metal bindingi287 – 2871Magnesium 3; via pros nitrogenImported
    Metal bindingi292 – 2921Magnesium 2
    Metal bindingi321 – 3211Magnesium 3; via carbonyl oxygenImported
    Metal bindingi326 – 3261Magnesium 2
    Metal bindingi412 – 4121Magnesium 4Imported

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. trehalose biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    TransferaseImported

    Keywords - Ligandi

    MagnesiumImported, Metal-bindingImported

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13774.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Putative glycosyltransferaseImported
    Gene namesi
    Name:vldEImported
    OrganismiStreptomyces hygroscopicus subsp. limoneusImported
    Taxonomic identifieri264445 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3T5TX-ray1.70A/B2-497[»]
    3T7DX-ray1.70A/B2-497[»]
    3VDMX-ray1.98A/B1-497[»]
    3VDNX-ray2.55B1-497[»]
    4F96X-ray2.15A/B1-497[»]
    4F97X-ray2.11A/B1-497[»]
    4F9FX-ray2.81A/B/C/D/E/F1-497[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q15JG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGSEIFLAS KRAAITYDTD PATGEPRAWL APGGTGNVVA EQAGVLNISW    50
    IASADSEDDR RASALNPDGV TMELHSGREI LVRLIRHDPA VFRNVQNFMT 100
    ANLMWAANNY GWDRWTQPSF GSDAREGWAD FGRFTRDFAD AILKSSAQSA 150
    DPVYLVHDYQ LVGVPALLRE QRPDAPILLF VHIPWPSADY WRILPKEIRT 200
    GILHGMLPAT TIGFFADRWC RNFLESVADL LPDARIDREA MTVEWRGHRT 250
    RLRTMPLGYS PLTLDGRNPQ LPEGIEEWAD GHRLVVHSGR TDPIKNAERA 300
    VRAFVLAARG GGLEKTRMLV RMNPNRLYVP ANADYVHRVE TAVAEANAEL 350
    GSDTVRIDND NDVNHTIACF RRADLLIFNS TVDGQNLSTF EAPLVNERDA 400
    DVILSETCGA AEVLGEYCRS VNPFDLVEQA EAISAALAAG PRQRAEAAAR 450
    RRDAARPWTL EAWVQAQLDG LAADHAARTA TAERFDTAPA VSTRADL 497
    Length:497
    Mass (Da):54,943
    Last modified:July 25, 2006 - v1
    Checksum:i91CDAB5D51B859DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ223652 Genomic DNA. Translation: ABC67269.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ223652 Genomic DNA. Translation: ABC67269.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3T5T X-ray 1.70 A/B 2-497 [» ]
    3T7D X-ray 1.70 A/B 2-497 [» ]
    3VDM X-ray 1.98 A/B 1-497 [» ]
    3VDN X-ray 2.55 B 1-497 [» ]
    4F96 X-ray 2.15 A/B 1-497 [» ]
    4F97 X-ray 2.11 A/B 1-497 [» ]
    4F9F X-ray 2.81 A/B/C/D/E/F 1-497 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13774.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from Streptomyces hygroscopicus var. limoneus KCCM 11405 (IFO 12704)."
      Singh D., Seo M.J., Kwon H.J., Rajkarnikar A., Kim K.R., Kim S.O., Suh J.W.
      Gene 376:13-23(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: KCCM 11405Imported.
    2. "Structural basis for the substrate specificity of ValL."
      Zhang H., Zheng L., Qian H.
      Submitted (JUL-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-497 IN COMPLEX WITH MAGNESIUM.
    3. "Structural basis for the substrate specificity of ValL."
      Zhang H., Zheng L., Qian H., Chen J.
      Submitted (JUL-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-497 IN COMPLEX WITH MAGNESIUM.
    4. "Mechanistic insights into validoxylamine A 7'-phosphate synthesis by VldE using the structure of the entire product complex."
      Cavalier M.C., Yim Y.S., Asamizu S., Neau D., Almabruk K.H., Mahmud T., Lee Y.H.
      PLoS ONE 7:e44934-e44934(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS).
    5. "Crystal Structure of the VldE, the pseudo-glycosyltransferase, which catalyzes non-glycosidic C-N coupling in Validamycin A biosynthesis."
      Cavalier M.C., Yim Y.-S., Asamizu S., Neau D., Mahmud T., Lee Y.-H.
      Submitted (JAN-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).
    6. "Crystal Structure of VldE, the pseudo-glycosyltransferase which catalyzes non-glycosidic C-N coupling in Validamycin A biosynthesis."
      Cavalier M.C., Yim Y.-S., Asamizu S., Neau D., Mahmud T., Lee Y.-H.
      Submitted (JAN-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS).

    Entry informationi

    Entry nameiQ15JG1_STRHY
    AccessioniPrimary (citable) accession number: Q15JG1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3