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Protein

Validamine 7-phosphate valienyltransferase

Gene

vldE

Organism
Streptomyces hygroscopicus subsp. limoneus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the antifungal agent validamycin A (PubMed:16725283). Catalyzes the condensation between GDP-valienol and validamine 7-phosphate via a nonglycosidic C-N bond formation to yield validoxylamine A 7'-phosphate (PubMed:21766819).2 Publications

Catalytic activityi

GDP-valienol + validamine 7-phosphate = validoxylamine A 7'-phosphate + GDP.1 Publication

Kineticsi

Kcat is 7 min(-1) with GDP-valienol as substrate. Kcat is 7 min(-1) with validamine 7-phosphate as substrate.1 Publication
  1. KM=59.7 µM for GDP-valienol1 Publication
  2. KM=308 µM for validamine 7-phosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei158GDP-valienolCombined sources1 Publication1
    Binding sitei182Validamine 7-phosphateCombined sources1 Publication1
    Binding sitei290GDP-valienolCombined sources1 Publication1
    Binding sitei295GDP-valienolCombined sources1 Publication1
    Binding sitei321GDP-valienolCombined sources1 Publication1
    Binding sitei366GDP-valienolCombined sources1 Publication1
    Binding sitei391GDP-valienolCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processAntibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13774

    Protein family/group databases

    CAZyiGT20 Glycosyltransferase Family 20

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Validamine 7-phosphate valienyltransferase1 Publication (EC:2.5.1.1351 Publication)
    Alternative name(s):
    Pseudoglycosyltransferase1 Publication
    Trehalose 6-phosphate synthase-like enzyme VldE1 Publication
    Gene namesi
    Name:vldE1 Publication
    ORF Names:SHL15_8006Imported
    OrganismiStreptomyces hygroscopicus subsp. limoneus
    Taxonomic identifieri264445 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
    Proteomesi
    • UP000057753 Componenti: Chromosome II

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004428461 – 497Validamine 7-phosphate valienyltransferaseAdd BLAST497

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1497
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 9Combined sources6
    Beta strandi15 – 19Combined sources5
    Turni21 – 23Combined sources3
    Beta strandi26 – 30Combined sources5
    Helixi35 – 40Combined sources6
    Beta strandi42 – 44Combined sources3
    Beta strandi45 – 53Combined sources9
    Helixi57 – 65Combined sources9
    Beta strandi70 – 73Combined sources4
    Helixi75 – 77Combined sources3
    Beta strandi79 – 85Combined sources7
    Helixi89 – 98Combined sources10
    Turni101 – 103Combined sources3
    Helixi104 – 108Combined sources5
    Beta strandi114 – 116Combined sources3
    Helixi122 – 145Combined sources24
    Turni146 – 148Combined sources3
    Beta strandi150 – 158Combined sources9
    Helixi159 – 161Combined sources3
    Helixi164 – 171Combined sources8
    Beta strandi173 – 175Combined sources3
    Beta strandi177 – 181Combined sources5
    Helixi188 – 191Combined sources4
    Helixi196 – 206Combined sources11
    Beta strandi209 – 216Combined sources8
    Helixi217 – 230Combined sources14
    Beta strandi235 – 237Combined sources3
    Turni238 – 241Combined sources4
    Beta strandi242 – 245Combined sources4
    Beta strandi248 – 254Combined sources7
    Helixi261 – 264Combined sources4
    Helixi275 – 279Combined sources5
    Beta strandi282 – 292Combined sources11
    Helixi293 – 295Combined sources3
    Helixi297 – 309Combined sources13
    Beta strandi316 – 323Combined sources8
    Helixi330 – 350Combined sources21
    Beta strandi354 – 359Combined sources6
    Helixi363 – 372Combined sources10
    Beta strandi374 – 378Combined sources5
    Beta strandi381 – 384Combined sources4
    Helixi388 – 395Combined sources8
    Beta strandi401 – 405Combined sources5
    Helixi411 – 414Combined sources4
    Helixi415 – 417Combined sources3
    Beta strandi418 – 421Combined sources4
    Helixi426 – 438Combined sources13
    Helixi441 – 455Combined sources15
    Helixi460 – 480Combined sources21

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3VDMX-ray1.98A/B1-497[»]
    3VDNX-ray2.55B1-497[»]
    4F96X-ray2.15A/B1-497[»]
    4F97X-ray2.11A/B1-497[»]
    4F9FX-ray2.81A/B/C/D/E/F1-497[»]
    SMRiQ15JG1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni325 – 326GDP-valienol bindingCombined sources1 Publication2
    Regioni361 – 362GDP-valienol bindingCombined sources1 Publication2
    Regioni383 – 386Validamine 7-phosphate bindingCombined sources1 Publication4
    Regioni387 – 388GDP-valienol bindingCombined sources1 Publication2

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Family and domain databases

    CDDicd03788 GT1_TPS, 1 hit
    InterProiView protein in InterPro
    IPR001830 Glyco_trans_20
    PfamiView protein in Pfam
    PF00982 Glyco_transf_20, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q15JG1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTGSEIFLAS KRAAITYDTD PATGEPRAWL APGGTGNVVA EQAGVLNISW
    60 70 80 90 100
    IASADSEDDR RASALNPDGV TMELHSGREI LVRLIRHDPA VFRNVQNFMT
    110 120 130 140 150
    ANLMWAANNY GWDRWTQPSF GSDAREGWAD FGRFTRDFAD AILKSSAQSA
    160 170 180 190 200
    DPVYLVHDYQ LVGVPALLRE QRPDAPILLF VHIPWPSADY WRILPKEIRT
    210 220 230 240 250
    GILHGMLPAT TIGFFADRWC RNFLESVADL LPDARIDREA MTVEWRGHRT
    260 270 280 290 300
    RLRTMPLGYS PLTLDGRNPQ LPEGIEEWAD GHRLVVHSGR TDPIKNAERA
    310 320 330 340 350
    VRAFVLAARG GGLEKTRMLV RMNPNRLYVP ANADYVHRVE TAVAEANAEL
    360 370 380 390 400
    GSDTVRIDND NDVNHTIACF RRADLLIFNS TVDGQNLSTF EAPLVNERDA
    410 420 430 440 450
    DVILSETCGA AEVLGEYCRS VNPFDLVEQA EAISAALAAG PRQRAEAAAR
    460 470 480 490
    RRDAARPWTL EAWVQAQLDG LAADHAARTA TAERFDTAPA VSTRADL
    Length:497
    Mass (Da):54,943
    Last modified:July 25, 2006 - v1
    Checksum:i91CDAB5D51B859DC
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ223652 Genomic DNA Translation: ABC67269.1
    CP013220 Genomic DNA Translation: ALO98983.1
    RefSeqiWP_014668900.1, NZ_CP013220.1

    Genome annotation databases

    EnsemblBacteriaiALO98983; ALO98983; SHL15_8006
    PATRICifig|264445.3.peg.8517

    Similar proteinsi

    Entry informationi

    Entry nameiVLDE_STRHL
    AccessioniPrimary (citable) accession number: Q15JG1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2018
    Last sequence update: July 25, 2006
    Last modified: March 28, 2018
    This is version 55 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome
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    Main funding by: National Institutes of Health