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Protein

Eugenol synthase 1

Gene

EGS1

Organism
Ocimum basilicum (Sweet basil)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of the phenylpropene eugenol from coniferyl acetate. Phenylpropenes are produced by plants as defense compounds with antimicrobial and antianimal properties, or as floral attractants of pollinators. Eugenol is a characteristic aromatic constituent of spices.1 Publication

Catalytic activityi

Eugenol + acetic acid + NADP+ = coniferyl acetate + NADPH.1 Publication
Eugenol + a carboxylate + NADP+ = a coniferyl ester + NADPH.1 Publication

Enzyme regulationi

Inhibited by zinc and copper ions. Repressed by 4-bromo-cinnamyl acetate.1 Publication

Kineticsi

  1. KM=5.1 mM for coniferyl acetate (at pH 6.5 and 28 degrees Celsius)1 Publication
  2. KM=131 µM for NADPH (at pH 6.5 and 28 degrees Celsius)1 Publication
  1. Vmax=19.9 nmol/sec/mg enzyme (at pH 6.5 and 28 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi: phenylpropanoid biosynthesis

This protein is involved in the pathway phenylpropanoid biosynthesis, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylpropanoid biosynthesis and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Phenylpropanoid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13834.
BRENDAi1.1.1.318. 4385.
SABIO-RKQ15GI4.
UniPathwayiUPA00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Eugenol synthase 1 (EC:1.1.1.318)
Gene namesi
Name:EGS1
OrganismiOcimum basilicum (Sweet basil)
Taxonomic identifieri39350 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeOcimeaeOcimum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003145761 – 314Eugenol synthase 1Add BLAST314

Proteomic databases

PRIDEiQ15GI4.

Expressioni

Tissue specificityi

Trichome glands.1 Publication

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Turni14 – 16Combined sources3
Helixi20 – 29Combined sources10
Beta strandi34 – 38Combined sources5
Helixi45 – 53Combined sources9
Beta strandi57 – 60Combined sources4
Helixi66 – 73Combined sources8
Beta strandi77 – 81Combined sources5
Helixi85 – 87Combined sources3
Turni88 – 90Combined sources3
Helixi91 – 101Combined sources11
Beta strandi106 – 108Combined sources3
Turni116 – 118Combined sources3
Helixi123 – 141Combined sources19
Beta strandi146 – 150Combined sources5
Helixi155 – 163Combined sources9
Beta strandi170 – 175Combined sources6
Beta strandi181 – 185Combined sources5
Helixi187 – 197Combined sources11
Helixi201 – 203Combined sources3
Beta strandi206 – 209Combined sources4
Helixi213 – 215Combined sources3
Beta strandi216 – 218Combined sources3
Helixi219 – 230Combined sources12
Beta strandi235 – 239Combined sources5
Helixi241 – 250Combined sources10
Turni253 – 255Combined sources3
Helixi256 – 266Combined sources11
Turni270 – 272Combined sources3
Helixi283 – 285Combined sources3
Helixi295 – 304Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QW8X-ray1.60A/B1-314[»]
2QX7X-ray1.75A/B1-314[»]
2QYSX-ray1.80A/B1-314[»]
2QZZX-ray1.60A/B1-314[»]
2R2GX-ray1.80A/B1-314[»]
2R6JX-ray1.50A/B1-314[»]
3C3XX-ray2.15A/B1-314[»]
ProteinModelPortaliQ15GI4.
SMRiQ15GI4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15GI4.

Family & Domainsi

Sequence similaritiesi

Belongs to the NmrA-type oxidoreductase family.Curated

Phylogenomic databases

KOiK17055.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q15GI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENGMKSKI LIFGGTGYIG NHMVKGSLKL GHPTYVFTRP NSSKTTLLDE
60 70 80 90 100
FQSLGAIIVK GELDEHEKLV ELMKKVDVVI SALAFPQILD QFKILEAIKV
110 120 130 140 150
AGNIKRFLPS DFGVEEDRIN ALPPFEALIE RKRMIRRAIE EANIPYTYVS
160 170 180 190 200
ANCFASYFIN YLLRPYDPKD EITVYGTGEA KFAMNYEQDI GLYTIKVATD
210 220 230 240 250
PRALNRVVIY RPSTNIITQL ELISRWEKKI GKKFKKIHVP EEEIVALTKE
260 270 280 290 300
LPEPENIPIA ILHCLFIDGA TMSYDFKEND VEASTLYPEL KFTTIDELLD
310
IFVHDPPPPA SAAF
Length:314
Mass (Da):35,607
Last modified:July 25, 2006 - v1
Checksum:iB221B0E8E733213B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372812 mRNA. Translation: ABD17321.1.

Genome annotation databases

KEGGiag:ABD17321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372812 mRNA. Translation: ABD17321.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QW8X-ray1.60A/B1-314[»]
2QX7X-ray1.75A/B1-314[»]
2QYSX-ray1.80A/B1-314[»]
2QZZX-ray1.60A/B1-314[»]
2R2GX-ray1.80A/B1-314[»]
2R6JX-ray1.50A/B1-314[»]
3C3XX-ray2.15A/B1-314[»]
ProteinModelPortaliQ15GI4.
SMRiQ15GI4.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ15GI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:ABD17321.

Phylogenomic databases

KOiK17055.

Enzyme and pathway databases

UniPathwayiUPA00711.
BioCyciMetaCyc:MONOMER-13834.
BRENDAi1.1.1.318. 4385.
SABIO-RKQ15GI4.

Miscellaneous databases

EvolutionaryTraceiQ15GI4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEGS1_OCIBA
AccessioniPrimary (citable) accession number: Q15GI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.