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Protein

Eugenol synthase 1

Gene

EGS1

Organism
Ocimum basilicum (Sweet basil)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of the phenylpropene eugenol from coniferyl acetate. Phenylpropenes are produced by plants as defense compounds with antimicrobial and antianimal properties, or as floral attractants of pollinators. Eugenol is a characteristic aromatic constituent of spices.1 Publication

Catalytic activityi

Eugenol + acetic acid + NADP+ = coniferyl acetate + NADPH.1 Publication
Eugenol + a carboxylate + NADP+ = a coniferyl ester + NADPH.1 Publication

Enzyme regulationi

Inhibited by zinc and copper ions. Repressed by 4-bromo-cinnamyl acetate.1 Publication

Kineticsi

  1. KM=5.1 mM for coniferyl acetate (at pH 6.5 and 28 degrees Celsius)1 Publication
  2. KM=131 µM for NADPH (at pH 6.5 and 28 degrees Celsius)1 Publication
  1. Vmax=19.9 nmol/sec/mg enzyme (at pH 6.5 and 28 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Pathwayi: phenylpropanoid biosynthesis

This protein is involved in the pathway phenylpropanoid biosynthesis, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway phenylpropanoid biosynthesis and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Phenylpropanoid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13834.
BRENDAi1.1.1.318. 4385.
SABIO-RKQ15GI4.
UniPathwayiUPA00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Eugenol synthase 1 (EC:1.1.1.318)
Gene namesi
Name:EGS1
OrganismiOcimum basilicum (Sweet basil)
Taxonomic identifieri39350 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeOcimeaeOcimum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Eugenol synthase 1PRO_0000314576Add
BLAST

Expressioni

Tissue specificityi

Trichome glands.1 Publication

Structurei

Secondary structure

1
314
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 134Combined sources
Turni14 – 163Combined sources
Helixi20 – 2910Combined sources
Beta strandi34 – 385Combined sources
Helixi45 – 539Combined sources
Beta strandi57 – 604Combined sources
Helixi66 – 738Combined sources
Beta strandi77 – 815Combined sources
Helixi85 – 873Combined sources
Turni88 – 903Combined sources
Helixi91 – 10111Combined sources
Beta strandi106 – 1083Combined sources
Turni116 – 1183Combined sources
Helixi123 – 14119Combined sources
Beta strandi146 – 1505Combined sources
Helixi155 – 1639Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi181 – 1855Combined sources
Helixi187 – 19711Combined sources
Helixi201 – 2033Combined sources
Beta strandi206 – 2094Combined sources
Helixi213 – 2153Combined sources
Beta strandi216 – 2183Combined sources
Helixi219 – 23012Combined sources
Beta strandi235 – 2395Combined sources
Helixi241 – 25010Combined sources
Turni253 – 2553Combined sources
Helixi256 – 26611Combined sources
Turni270 – 2723Combined sources
Helixi283 – 2853Combined sources
Helixi295 – 30410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QW8X-ray1.60A/B1-314[»]
2QX7X-ray1.75A/B1-314[»]
2QYSX-ray1.80A/B1-314[»]
2QZZX-ray1.60A/B1-314[»]
2R2GX-ray1.80A/B1-314[»]
2R6JX-ray1.50A/B1-314[»]
3C3XX-ray2.15A/B1-314[»]
ProteinModelPortaliQ15GI4.
SMRiQ15GI4. Positions 5-314.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15GI4.

Family & Domainsi

Sequence similaritiesi

Belongs to the NmrA-type oxidoreductase family.Curated

Phylogenomic databases

KOiK17055.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q15GI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENGMKSKI LIFGGTGYIG NHMVKGSLKL GHPTYVFTRP NSSKTTLLDE
60 70 80 90 100
FQSLGAIIVK GELDEHEKLV ELMKKVDVVI SALAFPQILD QFKILEAIKV
110 120 130 140 150
AGNIKRFLPS DFGVEEDRIN ALPPFEALIE RKRMIRRAIE EANIPYTYVS
160 170 180 190 200
ANCFASYFIN YLLRPYDPKD EITVYGTGEA KFAMNYEQDI GLYTIKVATD
210 220 230 240 250
PRALNRVVIY RPSTNIITQL ELISRWEKKI GKKFKKIHVP EEEIVALTKE
260 270 280 290 300
LPEPENIPIA ILHCLFIDGA TMSYDFKEND VEASTLYPEL KFTTIDELLD
310
IFVHDPPPPA SAAF
Length:314
Mass (Da):35,607
Last modified:July 25, 2006 - v1
Checksum:iB221B0E8E733213B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372812 mRNA. Translation: ABD17321.1.

Genome annotation databases

KEGGiag:ABD17321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372812 mRNA. Translation: ABD17321.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QW8X-ray1.60A/B1-314[»]
2QX7X-ray1.75A/B1-314[»]
2QYSX-ray1.80A/B1-314[»]
2QZZX-ray1.60A/B1-314[»]
2R2GX-ray1.80A/B1-314[»]
2R6JX-ray1.50A/B1-314[»]
3C3XX-ray2.15A/B1-314[»]
ProteinModelPortaliQ15GI4.
SMRiQ15GI4. Positions 5-314.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:ABD17321.

Phylogenomic databases

KOiK17055.

Enzyme and pathway databases

UniPathwayiUPA00711.
BioCyciMetaCyc:MONOMER-13834.
BRENDAi1.1.1.318. 4385.
SABIO-RKQ15GI4.

Miscellaneous databases

EvolutionaryTraceiQ15GI4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester."
    Koeduka T., Fridman E., Gang D.R., Vassao D.G., Jackson B.L., Kish C.M., Orlova I., Spassova S.M., Lewis N.G., Noel J.P., Baiga T.J., Dudareva N., Pichersky E.
    Proc. Natl. Acad. Sci. U.S.A. 103:10128-10133(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, CATALYTIC ACTIVITY.
    Strain: cv. SW.
    Tissue: Trichome gland.

Entry informationi

Entry nameiEGS1_OCIBA
AccessioniPrimary (citable) accession number: Q15GI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 25, 2006
Last modified: December 9, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.