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Q15853 (USF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Upstream stimulatory factor 2
Alternative name(s):
Class B basic helix-loop-helix protein 12
Short name=bHLHb12
FOS-interacting protein
Short name=FIP
Major late transcription factor 2
Upstream transcription factor 2
Gene names
Name:USF2
Synonyms:BHLHB12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Subunit structure

Interacts with MAF By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.

Subcellular location

Nucleus.

Tissue specificity

Ubiquitous.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlactation

Inferred from electronic annotation. Source: Ensembl

late viral transcription

Inferred by curator Ref.1. Source: BHF-UCL

lipid homeostasis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter by glucose

Inferred from mutant phenotype PubMed 7852331. Source: BHF-UCL

regulation of transcription from RNA polymerase II promoter by glucose

Inferred by curator Ref.1. Source: BHF-UCL

transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.1. Source: GOC

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 15358760Ref.1. Source: BHF-UCL

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype Ref.1. Source: BHF-UCL

bHLH transcription factor binding

Inferred from physical interaction Ref.1. Source: BHF-UCL

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 19505873. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction Ref.1. Source: BHF-UCL

protein homodimerization activity

Inferred from physical interaction Ref.1. Source: BHF-UCL

sequence-specific DNA binding

Inferred from direct assay Ref.1. Source: BHF-UCL

sequence-specific DNA binding transcription factor activity

Traceable author statement Ref.8Ref.2. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform USF2A (identifier: Q15853-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform USF2A-delta-H (identifier: Q15853-2)

The sequence of this isoform differs from the canonical sequence as follows:
     275-282: Missing.
Isoform USF2B (identifier: Q15853-3)

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.
Isoform USF2c (identifier: Q15853-4)

The sequence of this isoform differs from the canonical sequence as follows:
     76-206: Missing.
Note: Can bind as a homodimer to the E-box of the cathepsin B (CTSB) promoter.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Upstream stimulatory factor 2
PRO_0000127500

Regions

Domain235 – 29056bHLH
Region307 – 32822Leucine-zipper
Compositional bias11 – 2010Poly-Ala
Compositional bias245 – 2484Poly-Arg

Natural variations

Alternative sequence76 – 206131Missing in isoform USF2c.
VSP_047804
Alternative sequence77 – 14367Missing in isoform USF2B.
VSP_002164
Alternative sequence275 – 2828Missing in isoform USF2A-delta-H.
VSP_002165

Experimental info

Sequence conflict46 – 6419QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV Ref.7
Sequence conflict93 – 1008GDTAGAVS → EFHSWRRH in M77476. Ref.8
Sequence conflict1221A → V in M77476. Ref.8
Sequence conflict3081Q → R in CAG38742. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform USF2A [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 78CEFE97AC4C10CF

FASTA34636,955
        10         20         30         40         50         60 
MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG 

        70         80         90        100        110        120 
DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD 

       130        140        150        160        170        180 
GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA 

       190        200        210        220        230        240 
VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH 

       250        260        270        280        290        300 
NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 

       310        320        330        340 
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ 

« Hide

Isoform USF2A-delta-H [UniParc].

Checksum: 681B0AE58A84042B
Show »

FASTA33836,184
Isoform USF2B [UniParc].

Checksum: 15C53A2CC946CEA7
Show »

FASTA27930,570
Isoform USF2c [UniParc].

Checksum: E10CB131E46B58D2
Show »

FASTA21523,988

References

« Hide 'large scale' references
[1]"Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms."
Viollet B., Lefrancois-Martinez A.-M., Henrion A., Kahn A., Raymondjean M., Martinez A.
J. Biol. Chem. 271:1405-1415(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A; USF2B AND USF2A-DELTA-H).
Tissue: Liver.
[2]"Structure, sequence, and chromosome 19 localization of human USF2 and its rearrangement in a patient with multicystic renal dysplasia."
Groenen P.M.A., Garcia E., Debeer P., Devriendt K., Fryns J.-P., van de Ven W.J.M.
Genomics 38:141-148(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]"Isolation of a novel USF2 isoform: repressor of cathepsin B expression."
Yan S., Sloane B.F.
Gene 337:199-206(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM USF2C).
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
Tissue: Blood.
[7]"Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers."
Sirito M., Walker S., Lin Q., Kozlowski M.T., Klein W.H., Sawadogo M.
Gene Expr. 2:231-240(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 46-346.
Tissue: B-cell.
[8]"Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos."
Blanar M.A., Rutter W.J.
Science 256:1014-1018(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 93-346.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90824 mRNA. Translation: CAA62339.1.
X90825 mRNA. Translation: CAA62340.1.
X90826 mRNA. Translation: CAA62341.1.
Y07661 Genomic DNA. Translation: CAA68942.1.
AY147880 mRNA. Translation: AAN63092.1.
CR536504 mRNA. Translation: CAG38742.1.
AD000684 Genomic DNA. Translation: AAB51179.1.
BC049821 mRNA. Translation: AAH49821.1.
S50537 mRNA. Translation: AAB24368.1.
M77476 mRNA. No translation available.
CCDSCCDS12452.1. [Q15853-1]
CCDS12453.1. [Q15853-3]
PIRI54074.
RefSeqNP_003358.1. NM_003367.2. [Q15853-1]
NP_997174.1. NM_207291.1. [Q15853-3]
UniGeneHs.454534.

3D structure databases

ProteinModelPortalQ15853.
SMRQ15853. Positions 233-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113238. 18 interactions.
IntActQ15853. 10 interactions.
STRING9606.ENSP00000222305.

PTM databases

PhosphoSiteQ15853.

Polymorphism databases

DMDM2833271.

Proteomic databases

MaxQBQ15853.
PaxDbQ15853.
PRIDEQ15853.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222305; ENSP00000222305; ENSG00000105698. [Q15853-1]
ENST00000343550; ENSP00000340633; ENSG00000105698. [Q15853-3]
ENST00000379134; ENSP00000368429; ENSG00000105698. [Q15853-4]
ENST00000595068; ENSP00000471099; ENSG00000105698. [Q15853-2]
GeneID7392.
KEGGhsa:7392.
UCSCuc002nyq.1. human. [Q15853-1]
uc002nyr.1. human. [Q15853-3]

Organism-specific databases

CTD7392.
GeneCardsGC19P035759.
HGNCHGNC:12594. USF2.
HPAHPA046567.
MIM600390. gene.
neXtProtNX_Q15853.
PharmGKBPA37224.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG272240.
HOVERGENHBG004346.
InParanoidQ15853.
KOK09106.
OrthoDBEOG7GXPBW.
PhylomeDBQ15853.
TreeFamTF323338.

Enzyme and pathway databases

SignaLinkQ15853.

Gene expression databases

ArrayExpressQ15853.
BgeeQ15853.
CleanExHS_USF2.
GenevestigatorQ15853.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSF2. human.
GeneWikiUSF2.
GenomeRNAi7392.
NextBio28944.
PROQ15853.
SOURCESearch...

Entry information

Entry nameUSF2_HUMAN
AccessionPrimary (citable) accession number: Q15853
Secondary accession number(s): O00671 expand/collapse secondary AC list , O00709, Q05750, Q07952, Q15851, Q15852, Q6FI33, Q6YI47
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM