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Protein

Upstream stimulatory factor 2

Gene

USF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

Caution

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • DNA binding transcription factor activity Source: ProtInc
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: BHF-UCL

GO - Biological processi

  • lactation Source: Ensembl
  • late viral transcription Source: BHF-UCL
  • lipid homeostasis Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  • transcription by RNA polymerase II Source: BHF-UCL

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiQ15853
SIGNORiQ15853

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 2
Alternative name(s):
Class B basic helix-loop-helix protein 12
Short name:
bHLHb12
FOS-interacting protein
Short name:
FIP
Major late transcription factor 2
Upstream transcription factor 2
Gene namesi
Name:USF2
Synonyms:BHLHB12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105698.15
HGNCiHGNC:12594 USF2
MIMi600390 gene
neXtProtiNX_Q15853

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi7392
OpenTargetsiENSG00000105698
PharmGKBiPA37224

Polymorphism and mutation databases

BioMutaiUSF2
DMDMi2833271

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275001 – 346Upstream stimulatory factor 2Add BLAST346

Proteomic databases

EPDiQ15853
MaxQBiQ15853
PaxDbiQ15853
PeptideAtlasiQ15853
PRIDEiQ15853

PTM databases

iPTMnetiQ15853
PhosphoSitePlusiQ15853

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000105698
CleanExiHS_USF2
ExpressionAtlasiQ15853 baseline and differential
GenevisibleiQ15853 HS

Organism-specific databases

HPAiHPA029764
HPA046567

Interactioni

Subunit structurei

Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • bHLH transcription factor binding Source: BHF-UCL
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

BioGridi11323828 interactors.
CORUMiQ15853
IntActiQ15853 28 interactors.
MINTiQ15853
STRINGi9606.ENSP00000222305

Structurei

3D structure databases

ProteinModelPortaliQ15853
SMRiQ15853
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini235 – 290bHLHPROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 328Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 20Poly-Ala10
Compositional biasi245 – 248Poly-Arg4

Phylogenomic databases

eggNOGiENOG410INSC Eukaryota
ENOG4111DV1 LUCA
GeneTreeiENSGT00390000004498
HOGENOMiHOG000294174
HOVERGENiHBG004346
InParanoidiQ15853
KOiK09106
PhylomeDBiQ15853
TreeFamiTF323338

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.101 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform USF2A (identifier: Q15853-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ
Length:346
Mass (Da):36,955
Last modified:November 1, 1996 - v1
Checksum:i78CEFE97AC4C10CF
GO
Isoform USF2A-delta-H (identifier: Q15853-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-282: Missing.

Show »
Length:338
Mass (Da):36,184
Checksum:i681B0AE58A84042B
GO
Isoform USF2B (identifier: Q15853-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Show »
Length:279
Mass (Da):30,570
Checksum:i15C53A2CC946CEA7
GO
Isoform USF2c (identifier: Q15853-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-206: Missing.

Note: Can bind as a homodimer to the E-box of the cathepsin B (CTSB) promoter.
Show »
Length:215
Mass (Da):23,988
Checksum:iE10CB131E46B58D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46 – 64QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV (PubMed:1450663).CuratedAdd BLAST19
Sequence conflicti93 – 100GDTAGAVS → EFHSWRRH in M77476 (PubMed:1589769).Curated8
Sequence conflicti122A → V in M77476 (PubMed:1589769).Curated1
Sequence conflicti308Q → R in CAG38742 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04780476 – 206Missing in isoform USF2c. 1 PublicationAdd BLAST131
Alternative sequenceiVSP_00216477 – 143Missing in isoform USF2B. 1 PublicationAdd BLAST67
Alternative sequenceiVSP_002165275 – 282Missing in isoform USF2A-delta-H. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90824 mRNA Translation: CAA62339.1
X90825 mRNA Translation: CAA62340.1
X90826 mRNA Translation: CAA62341.1
Y07661 Genomic DNA Translation: CAA68942.1
AY147880 mRNA Translation: AAN63092.1
CR536504 mRNA Translation: CAG38742.1
AD000684 Genomic DNA Translation: AAB51179.1
BC049821 mRNA Translation: AAH49821.1
S50537 mRNA Translation: AAB24368.1
M77476 mRNA No translation available.
CCDSiCCDS12452.1 [Q15853-1]
CCDS12453.1 [Q15853-3]
CCDS82329.1 [Q15853-4]
PIRiI54074
RefSeqiNP_001308079.1, NM_001321150.1 [Q15853-4]
NP_003358.1, NM_003367.3 [Q15853-1]
NP_997174.1, NM_207291.2 [Q15853-3]
UniGeneiHs.454534

Genome annotation databases

EnsembliENST00000222305; ENSP00000222305; ENSG00000105698 [Q15853-1]
ENST00000343550; ENSP00000340633; ENSG00000105698 [Q15853-3]
ENST00000379134; ENSP00000368429; ENSG00000105698 [Q15853-4]
ENST00000595068; ENSP00000471099; ENSG00000105698 [Q15853-2]
GeneIDi7392
KEGGihsa:7392
UCSCiuc002nyq.2 human [Q15853-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUSF2_HUMAN
AccessioniPrimary (citable) accession number: Q15853
Secondary accession number(s): O00671
, O00709, Q05750, Q07952, Q15851, Q15852, Q6FI33, Q6YI47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome