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Q15853

- USF2_HUMAN

UniProt

Q15853 - USF2_HUMAN

Protein

Upstream stimulatory factor 2

Gene

USF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

    GO - Molecular functioni

    1. bHLH transcription factor binding Source: BHF-UCL
    2. double-stranded DNA binding Source: Ensembl
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: BHF-UCL
    5. protein homodimerization activity Source: BHF-UCL
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
    7. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    8. sequence-specific DNA binding Source: BHF-UCL
    9. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. lactation Source: Ensembl
    2. late viral transcription Source: BHF-UCL
    3. lipid homeostasis Source: BHF-UCL
    4. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    5. positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
    6. regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
    7. transcription, DNA-templated Source: UniProtKB-KW
    8. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiQ15853.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Upstream stimulatory factor 2
    Alternative name(s):
    Class B basic helix-loop-helix protein 12
    Short name:
    bHLHb12
    FOS-interacting protein
    Short name:
    FIP
    Major late transcription factor 2
    Upstream transcription factor 2
    Gene namesi
    Name:USF2
    Synonyms:BHLHB12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:12594. USF2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37224.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Upstream stimulatory factor 2PRO_0000127500Add
    BLAST

    Proteomic databases

    MaxQBiQ15853.
    PaxDbiQ15853.
    PRIDEiQ15853.

    PTM databases

    PhosphoSiteiQ15853.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiQ15853.
    BgeeiQ15853.
    CleanExiHS_USF2.
    GenevestigatoriQ15853.

    Organism-specific databases

    HPAiHPA046567.

    Interactioni

    Subunit structurei

    Interacts with MAF By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.By similarity

    Protein-protein interaction databases

    BioGridi113238. 18 interactions.
    IntActiQ15853. 10 interactions.
    STRINGi9606.ENSP00000222305.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15853.
    SMRiQ15853. Positions 233-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini235 – 29056bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni307 – 32822Leucine-zipperAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 2010Poly-Ala
    Compositional biasi245 – 2484Poly-Arg

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG272240.
    HOVERGENiHBG004346.
    InParanoidiQ15853.
    KOiK09106.
    OrthoDBiEOG7GXPBW.
    PhylomeDBiQ15853.
    TreeFamiTF323338.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform USF2A (identifier: Q15853-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA    50
    ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS 100
    VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF 150
    SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM 200
    TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK 250
    INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 300
    FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ 346
    Length:346
    Mass (Da):36,955
    Last modified:November 1, 1996 - v1
    Checksum:i78CEFE97AC4C10CF
    GO
    Isoform USF2A-delta-H (identifier: Q15853-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         275-282: Missing.

    Show »
    Length:338
    Mass (Da):36,184
    Checksum:i681B0AE58A84042B
    GO
    Isoform USF2B (identifier: Q15853-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         77-143: Missing.

    Show »
    Length:279
    Mass (Da):30,570
    Checksum:i15C53A2CC946CEA7
    GO
    Isoform USF2c (identifier: Q15853-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         76-206: Missing.

    Note: Can bind as a homodimer to the E-box of the cathepsin B (CTSB) promoter.

    Show »
    Length:215
    Mass (Da):23,988
    Checksum:iE10CB131E46B58D2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 6419QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV(PubMed:1450663)CuratedAdd
    BLAST
    Sequence conflicti93 – 1008GDTAGAVS → EFHSWRRH in M77476. (PubMed:1589769)Curated
    Sequence conflicti122 – 1221A → V in M77476. (PubMed:1589769)Curated
    Sequence conflicti308 – 3081Q → R in CAG38742. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei76 – 206131Missing in isoform USF2c. 1 PublicationVSP_047804Add
    BLAST
    Alternative sequencei77 – 14367Missing in isoform USF2B. 1 PublicationVSP_002164Add
    BLAST
    Alternative sequencei275 – 2828Missing in isoform USF2A-delta-H. 1 PublicationVSP_002165

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90824 mRNA. Translation: CAA62339.1.
    X90825 mRNA. Translation: CAA62340.1.
    X90826 mRNA. Translation: CAA62341.1.
    Y07661 Genomic DNA. Translation: CAA68942.1.
    AY147880 mRNA. Translation: AAN63092.1.
    CR536504 mRNA. Translation: CAG38742.1.
    AD000684 Genomic DNA. Translation: AAB51179.1.
    BC049821 mRNA. Translation: AAH49821.1.
    S50537 mRNA. Translation: AAB24368.1.
    M77476 mRNA. No translation available.
    CCDSiCCDS12452.1. [Q15853-1]
    CCDS12453.1. [Q15853-3]
    PIRiI54074.
    RefSeqiNP_003358.1. NM_003367.2. [Q15853-1]
    NP_997174.1. NM_207291.1. [Q15853-3]
    UniGeneiHs.454534.

    Genome annotation databases

    EnsembliENST00000222305; ENSP00000222305; ENSG00000105698. [Q15853-1]
    ENST00000343550; ENSP00000340633; ENSG00000105698. [Q15853-3]
    ENST00000379134; ENSP00000368429; ENSG00000105698. [Q15853-4]
    ENST00000595068; ENSP00000471099; ENSG00000105698. [Q15853-2]
    GeneIDi7392.
    KEGGihsa:7392.
    UCSCiuc002nyq.1. human. [Q15853-1]
    uc002nyr.1. human. [Q15853-3]

    Polymorphism databases

    DMDMi2833271.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90824 mRNA. Translation: CAA62339.1 .
    X90825 mRNA. Translation: CAA62340.1 .
    X90826 mRNA. Translation: CAA62341.1 .
    Y07661 Genomic DNA. Translation: CAA68942.1 .
    AY147880 mRNA. Translation: AAN63092.1 .
    CR536504 mRNA. Translation: CAG38742.1 .
    AD000684 Genomic DNA. Translation: AAB51179.1 .
    BC049821 mRNA. Translation: AAH49821.1 .
    S50537 mRNA. Translation: AAB24368.1 .
    M77476 mRNA. No translation available.
    CCDSi CCDS12452.1. [Q15853-1 ]
    CCDS12453.1. [Q15853-3 ]
    PIRi I54074.
    RefSeqi NP_003358.1. NM_003367.2. [Q15853-1 ]
    NP_997174.1. NM_207291.1. [Q15853-3 ]
    UniGenei Hs.454534.

    3D structure databases

    ProteinModelPortali Q15853.
    SMRi Q15853. Positions 233-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113238. 18 interactions.
    IntActi Q15853. 10 interactions.
    STRINGi 9606.ENSP00000222305.

    PTM databases

    PhosphoSitei Q15853.

    Polymorphism databases

    DMDMi 2833271.

    Proteomic databases

    MaxQBi Q15853.
    PaxDbi Q15853.
    PRIDEi Q15853.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000222305 ; ENSP00000222305 ; ENSG00000105698 . [Q15853-1 ]
    ENST00000343550 ; ENSP00000340633 ; ENSG00000105698 . [Q15853-3 ]
    ENST00000379134 ; ENSP00000368429 ; ENSG00000105698 . [Q15853-4 ]
    ENST00000595068 ; ENSP00000471099 ; ENSG00000105698 . [Q15853-2 ]
    GeneIDi 7392.
    KEGGi hsa:7392.
    UCSCi uc002nyq.1. human. [Q15853-1 ]
    uc002nyr.1. human. [Q15853-3 ]

    Organism-specific databases

    CTDi 7392.
    GeneCardsi GC19P035759.
    HGNCi HGNC:12594. USF2.
    HPAi HPA046567.
    MIMi 600390. gene.
    neXtProti NX_Q15853.
    PharmGKBi PA37224.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG272240.
    HOVERGENi HBG004346.
    InParanoidi Q15853.
    KOi K09106.
    OrthoDBi EOG7GXPBW.
    PhylomeDBi Q15853.
    TreeFami TF323338.

    Enzyme and pathway databases

    SignaLinki Q15853.

    Miscellaneous databases

    ChiTaRSi USF2. human.
    GeneWikii USF2.
    GenomeRNAii 7392.
    NextBioi 28944.
    PROi Q15853.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15853.
    Bgeei Q15853.
    CleanExi HS_USF2.
    Genevestigatori Q15853.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms."
      Viollet B., Lefrancois-Martinez A.-M., Henrion A., Kahn A., Raymondjean M., Martinez A.
      J. Biol. Chem. 271:1405-1415(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A; USF2B AND USF2A-DELTA-H).
      Tissue: Liver.
    2. "Structure, sequence, and chromosome 19 localization of human USF2 and its rearrangement in a patient with multicystic renal dysplasia."
      Groenen P.M.A., Garcia E., Debeer P., Devriendt K., Fryns J.-P., van de Ven W.J.M.
      Genomics 38:141-148(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    3. "Isolation of a novel USF2 isoform: repressor of cathepsin B expression."
      Yan S., Sloane B.F.
      Gene 337:199-206(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM USF2C).
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
    5. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
      Tissue: Blood.
    7. "Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers."
      Sirito M., Walker S., Lin Q., Kozlowski M.T., Klein W.H., Sawadogo M.
      Gene Expr. 2:231-240(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 46-346.
      Tissue: B-cell.
    8. "Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos."
      Blanar M.A., Rutter W.J.
      Science 256:1014-1018(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 93-346.

    Entry informationi

    Entry nameiUSF2_HUMAN
    AccessioniPrimary (citable) accession number: Q15853
    Secondary accession number(s): O00671
    , O00709, Q05750, Q07952, Q15851, Q15852, Q6FI33, Q6YI47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3