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Q15853

- USF2_HUMAN

UniProt

Q15853 - USF2_HUMAN

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Protein

Upstream stimulatory factor 2

Gene
USF2, BHLHB12
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functioni

  1. bHLH transcription factor binding Source: BHF-UCL
  2. double-stranded DNA binding Source: Ensembl
  3. protein binding Source: UniProtKB
  4. protein heterodimerization activity Source: BHF-UCL
  5. protein homodimerization activity Source: BHF-UCL
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  7. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  8. sequence-specific DNA binding Source: BHF-UCL
  9. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. lactation Source: Ensembl
  2. late viral transcription Source: BHF-UCL
  3. lipid homeostasis Source: BHF-UCL
  4. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  5. positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  6. regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
  7. transcription, DNA-templated Source: UniProtKB-KW
  8. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ15853.

Names & Taxonomyi

Protein namesi
Recommended name:
Upstream stimulatory factor 2
Alternative name(s):
Class B basic helix-loop-helix protein 12
Short name:
bHLHb12
FOS-interacting protein
Short name:
FIP
Major late transcription factor 2
Upstream transcription factor 2
Gene namesi
Name:USF2
Synonyms:BHLHB12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:12594. USF2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37224.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Upstream stimulatory factor 2PRO_0000127500Add
BLAST

Proteomic databases

MaxQBiQ15853.
PaxDbiQ15853.
PRIDEiQ15853.

PTM databases

PhosphoSiteiQ15853.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

ArrayExpressiQ15853.
BgeeiQ15853.
CleanExiHS_USF2.
GenevestigatoriQ15853.

Organism-specific databases

HPAiHPA046567.

Interactioni

Subunit structurei

Interacts with MAF By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.

Protein-protein interaction databases

BioGridi113238. 18 interactions.
IntActiQ15853. 10 interactions.
STRINGi9606.ENSP00000222305.

Structurei

3D structure databases

ProteinModelPortaliQ15853.
SMRiQ15853. Positions 233-296.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini235 – 29056bHLHAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 32822Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 2010Poly-Ala
Compositional biasi245 – 2484Poly-Arg

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG272240.
HOVERGENiHBG004346.
InParanoidiQ15853.
KOiK09106.
OrthoDBiEOG7GXPBW.
PhylomeDBiQ15853.
TreeFamiTF323338.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform USF2A (identifier: Q15853-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA    50
ITSVQQAAFG DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS 100
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF 150
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM 200
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK 250
INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 300
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ 346
Length:346
Mass (Da):36,955
Last modified:November 1, 1996 - v1
Checksum:i78CEFE97AC4C10CF
GO
Isoform USF2A-delta-H (identifier: Q15853-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-282: Missing.

Show »
Length:338
Mass (Da):36,184
Checksum:i681B0AE58A84042B
GO
Isoform USF2B (identifier: Q15853-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-143: Missing.

Show »
Length:279
Mass (Da):30,570
Checksum:i15C53A2CC946CEA7
GO
Isoform USF2c (identifier: Q15853-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-206: Missing.

Note: Can bind as a homodimer to the E-box of the cathepsin B (CTSB) promoter.

Show »
Length:215
Mass (Da):23,988
Checksum:iE10CB131E46B58D2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei76 – 206131Missing in isoform USF2c. VSP_047804Add
BLAST
Alternative sequencei77 – 14367Missing in isoform USF2B. VSP_002164Add
BLAST
Alternative sequencei275 – 2828Missing in isoform USF2A-delta-H. VSP_002165

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 6419QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV1 PublicationAdd
BLAST
Sequence conflicti93 – 1008GDTAGAVS → EFHSWRRH in M77476. 1 Publication
Sequence conflicti122 – 1221A → V in M77476. 1 Publication
Sequence conflicti308 – 3081Q → R in CAG38742. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90824 mRNA. Translation: CAA62339.1.
X90825 mRNA. Translation: CAA62340.1.
X90826 mRNA. Translation: CAA62341.1.
Y07661 Genomic DNA. Translation: CAA68942.1.
AY147880 mRNA. Translation: AAN63092.1.
CR536504 mRNA. Translation: CAG38742.1.
AD000684 Genomic DNA. Translation: AAB51179.1.
BC049821 mRNA. Translation: AAH49821.1.
S50537 mRNA. Translation: AAB24368.1.
M77476 mRNA. No translation available.
CCDSiCCDS12452.1. [Q15853-1]
CCDS12453.1. [Q15853-3]
PIRiI54074.
RefSeqiNP_003358.1. NM_003367.2. [Q15853-1]
NP_997174.1. NM_207291.1. [Q15853-3]
UniGeneiHs.454534.

Genome annotation databases

EnsembliENST00000222305; ENSP00000222305; ENSG00000105698. [Q15853-1]
ENST00000343550; ENSP00000340633; ENSG00000105698. [Q15853-3]
ENST00000379134; ENSP00000368429; ENSG00000105698. [Q15853-4]
ENST00000595068; ENSP00000471099; ENSG00000105698. [Q15853-2]
GeneIDi7392.
KEGGihsa:7392.
UCSCiuc002nyq.1. human. [Q15853-1]
uc002nyr.1. human. [Q15853-3]

Polymorphism databases

DMDMi2833271.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90824 mRNA. Translation: CAA62339.1 .
X90825 mRNA. Translation: CAA62340.1 .
X90826 mRNA. Translation: CAA62341.1 .
Y07661 Genomic DNA. Translation: CAA68942.1 .
AY147880 mRNA. Translation: AAN63092.1 .
CR536504 mRNA. Translation: CAG38742.1 .
AD000684 Genomic DNA. Translation: AAB51179.1 .
BC049821 mRNA. Translation: AAH49821.1 .
S50537 mRNA. Translation: AAB24368.1 .
M77476 mRNA. No translation available.
CCDSi CCDS12452.1. [Q15853-1 ]
CCDS12453.1. [Q15853-3 ]
PIRi I54074.
RefSeqi NP_003358.1. NM_003367.2. [Q15853-1 ]
NP_997174.1. NM_207291.1. [Q15853-3 ]
UniGenei Hs.454534.

3D structure databases

ProteinModelPortali Q15853.
SMRi Q15853. Positions 233-296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113238. 18 interactions.
IntActi Q15853. 10 interactions.
STRINGi 9606.ENSP00000222305.

PTM databases

PhosphoSitei Q15853.

Polymorphism databases

DMDMi 2833271.

Proteomic databases

MaxQBi Q15853.
PaxDbi Q15853.
PRIDEi Q15853.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000222305 ; ENSP00000222305 ; ENSG00000105698 . [Q15853-1 ]
ENST00000343550 ; ENSP00000340633 ; ENSG00000105698 . [Q15853-3 ]
ENST00000379134 ; ENSP00000368429 ; ENSG00000105698 . [Q15853-4 ]
ENST00000595068 ; ENSP00000471099 ; ENSG00000105698 . [Q15853-2 ]
GeneIDi 7392.
KEGGi hsa:7392.
UCSCi uc002nyq.1. human. [Q15853-1 ]
uc002nyr.1. human. [Q15853-3 ]

Organism-specific databases

CTDi 7392.
GeneCardsi GC19P035759.
HGNCi HGNC:12594. USF2.
HPAi HPA046567.
MIMi 600390. gene.
neXtProti NX_Q15853.
PharmGKBi PA37224.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG272240.
HOVERGENi HBG004346.
InParanoidi Q15853.
KOi K09106.
OrthoDBi EOG7GXPBW.
PhylomeDBi Q15853.
TreeFami TF323338.

Enzyme and pathway databases

SignaLinki Q15853.

Miscellaneous databases

ChiTaRSi USF2. human.
GeneWikii USF2.
GenomeRNAii 7392.
NextBioi 28944.
PROi Q15853.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q15853.
Bgeei Q15853.
CleanExi HS_USF2.
Genevestigatori Q15853.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms."
    Viollet B., Lefrancois-Martinez A.-M., Henrion A., Kahn A., Raymondjean M., Martinez A.
    J. Biol. Chem. 271:1405-1415(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A; USF2B AND USF2A-DELTA-H).
    Tissue: Liver.
  2. "Structure, sequence, and chromosome 19 localization of human USF2 and its rearrangement in a patient with multicystic renal dysplasia."
    Groenen P.M.A., Garcia E., Debeer P., Devriendt K., Fryns J.-P., van de Ven W.J.M.
    Genomics 38:141-148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "Isolation of a novel USF2 isoform: repressor of cathepsin B expression."
    Yan S., Sloane B.F.
    Gene 337:199-206(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM USF2C).
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
  5. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
    Tissue: Blood.
  7. "Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers."
    Sirito M., Walker S., Lin Q., Kozlowski M.T., Klein W.H., Sawadogo M.
    Gene Expr. 2:231-240(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 46-346.
    Tissue: B-cell.
  8. "Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos."
    Blanar M.A., Rutter W.J.
    Science 256:1014-1018(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 93-346.

Entry informationi

Entry nameiUSF2_HUMAN
AccessioniPrimary (citable) accession number: Q15853
Secondary accession number(s): O00671
, O00709, Q05750, Q07952, Q15851, Q15852, Q6FI33, Q6YI47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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