Upstream stimulatory factor 2 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Upstream stimulatory factor 2
Alternative name(s):
    Upstream transcription factor 2
    FOS-interacting protein
      Short name=FIP
    Major late transcription factor 2
    Class B basic helix-loop-helix protein 12
      Short name=bHLHb12

Gene names

Name:	USF2
Synonyms:	BHLHB12

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Subunit structure	Interacts with MAF By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	late viral mRNA transcription Inferred by curator. Source: UniProtKB lipid homeostasis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of gene-specific transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter by glucose Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	RNA polymerase II transcription factor activity Ref.7 Traceable author statement. Source: ProtInc RNA polymerase II transcription factor activity, enhancer binding Inferred from mutant phenotype. Source: UniProtKB bHLH transcription factor binding Ref.1 Inferred from physical interaction. Source: UniProtKB protein heterodimerization activity Ref.1 Inferred from physical interaction. Source: UniProtKB protein homodimerization activity Ref.1 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Ref.1 Inferred from direct assay. Source: UniProtKB transcription factor activity Ref.2 Ref.7 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-1055994,EBI-1055994
BIRC5	O15392	1	EBI-1055994,EBI-518823
FUS	P35637	1	EBI-1055994,EBI-400434
HSPA4L	O95757	1	EBI-1055994,EBI-358652
NPM1	P06748	1	EBI-1055994,EBI-78579
PTP4A2	Q12974	1	EBI-1055994,EBI-1046324
S100A8	P05109	1	EBI-1055994,EBI-355281
S100A9	P06702	1	EBI-1055994,EBI-1055001
SERPINB3	P29508	1	EBI-1055994,EBI-359110
SERPINB4	P48594	1	EBI-1055994,EBI-1055490
USF1	P22415	1	EBI-1055994,EBI-1054489

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform USF2A (identifier: Q15853-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform USF2A-delta-H (identifier: Q15853-2) The sequence of this isoform differs from the canonical sequence as follows: 275-282: Missing.

Isoform USF2B (identifier: Q15853-3) The sequence of this isoform differs from the canonical sequence as follows: 77-143: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Upstream stimulatory factor 2

PRO_0000127500

Regions

Domain

249 – 291

43

Helix-loop-helix motif

Domain

307 – 328

22

Leucine-zipper

DNA binding

235 – 248

14

Basic motif

Compositional bias

11 – 20

10

Poly-Ala

Compositional bias

245 – 248

4

Poly-Arg

Amino acid modifications

Modified residue

293

1

Phosphothreonine Ref.8

Natural variations

Alternative sequence

77 – 143

67

Missing in isoform USF2B.

VSP_002164

Alternative sequence

275 – 282

8

Missing in isoform USF2A-delta-H.

VSP_002165

Experimental info

Sequence conflict

46 – 64

19

QTAVA…GDHNI → GGGTSGGRGSGIQTRVQHV Ref.6

Sequence conflict

93 – 100

8

GDTAGAVS → EFHSWRRH in M77476. Ref.7

Sequence conflict

122

1

A → V in M77476. Ref.7

Sequence conflict

308

1

Q → R in CAG38742. Ref.3

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Sequences

Sequence		Length	Mass (Da)
Isoform USF2A [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 78CEFE97AC4C10CF Show »	FASTA	346	36,955
10 20 30 40 50 60 MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG 70 80 90 100 110 120 DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD 130 140 150 160 170 180 GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA 190 200 210 220 230 240 VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH 250 260 270 280 290 300 NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 310 320 330 340 FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GEGTRQ « Hide
Isoform USF2A-delta-H. Checksum: 681B0AE58A84042B Show »	FASTA	338	36,184
10 20 30 40 50 60 MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG 70 80 90 100 110 120 DHNIQYQFRT ETNGGQVTYR VVQVTDGQLD GQGDTAGAVS VVSTAAFAGG QQAVTQVGVD 130 140 150 160 170 180 GAAQRPGPAA ASVPPGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA 190 200 210 220 230 240 VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH 250 260 270 280 290 300 NEVERRRRDK INNWIVQLSK IIPDCNADNS KTGAACDYIR ELRQTNQRMQ ETFKEAERLQ 310 320 330 MDNELLRQQI EELKNENALL RAQLQQHNLE MVGEGTRQ « Hide
Isoform USF2B. Checksum: 15C53A2CC946CEA7 Show »	FASTA	279	30,570
10 20 30 40 50 60 MDMLDPGLDP AASATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA ITSVQQAAFG 70 80 90 100 110 120 DHNIQYQFRT ETNGGQAVIQ NPFSNGGSPA AEAVSGEARF AYFPASSVGD TTAVSVQTTD 130 140 150 160 170 180 QSLQAGGQFY VMMTPQDVLQ TGTQRTIAPR THPYSPKIDG TRTPRDERRR AQHNEVERRR 190 200 210 220 230 240 RDKINNWIVQ LSKIIPDCNA DNSKTGASKG GILSKACDYI RELRQTNQRM QETFKEAERL 250 260 270 QMDNELLRQQ IEELKNENAL LRAQLQQHNL EMVGEGTRQ « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms." Viollet B., Lefrancois-Martinez A.-M., Henrion A., Kahn A., Raymondjean M., Martinez A. J. Biol. Chem. 271:1405-1415(1996) [PubMed: 8576131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS USF2A; USF2B AND USF2A-DELTA-H). Tissue: Liver.
[2]	"Structure, sequence, and chromosome 19 localization of human USF2 and its rearrangement in a patient with multicystic renal dysplasia." Groenen P.M.A., Garcia E., Debeer P., Devriendt K., Fryns J.-P., van de Ven W.J.M. Genomics 38:141-148(1996) [PubMed: 8954795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A).
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM USF2A). Tissue: Blood.
[6]	"Members of the USF family of helix-loop-helix proteins bind DNA as homo- as well as heterodimers." Sirito M., Walker S., Lin Q., Kozlowski M.T., Klein W.H., Sawadogo M. Gene Expr. 2:231-240(1992) [PubMed: 1450663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 46-346. Tissue: B-cell.
[7]	"Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos." Blanar M.A., Rutter W.J. Science 256:1014-1018(1992) [PubMed: 1589769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 93-346.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-293, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	X90824 mRNA. Translation: CAA62339.1. X90825 mRNA. Translation: CAA62340.1. X90826 mRNA. Translation: CAA62341.1. Y07661 Genomic DNA. Translation: CAA68942.1. CR536504 mRNA. Translation: CAG38742.1. AD000684 Genomic DNA. Translation: AAB51179.1. BC049821 mRNA. Translation: AAH49821.1. S50537 mRNA. Translation: AAB24368.1. M77476 mRNA. No translation available.
IPI	IPI00020037. IPI00216376. IPI00216377.
PIR	I54074.
RefSeq	NP_003358.1. NP_997174.1.
UniGene	Hs.454534
3D structure databases
SMR	Q15853. Positions 233-296, 234-322.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15853. 16 interactions.
STRING	Q15853.
PTM databases
PhosphoSite	Q15853.
Genome annotation databases
Ensembl	ENST00000222305; ENSP00000222305; ENSG00000105698; Homo sapiens. [Genome view]
GeneID	7392.
KEGG	hsa:7392.
UCSC	uc002nyq.1. human. uc002nyr.1. human. uc002nyv.1. human.
Organism-specific databases
CTD	7392.
GeneCards	GC19P040451.
H-InvDB	HIX0027525.
HGNC	HGNC:12594. USF2.
MIM	600390. gene.
PharmGKB	PA37224.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15978.
HOVERGEN	Q15853.
InParanoid	Q15853.
OMA	MVGESAR.
PhylomeDB	Q15853.
Gene expression databases
ArrayExpress	Q15853.
Bgee	Q15853.
CleanEx	HS_USF2.
Genevestigator	Q15853.
GermOnline	ENSG00000105698. Homo sapiens.
Family and domain databases
InterPro	IPR001092. HLH_DNA-bd_dom. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	28944.
SOURCE	Search...

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Entry information

Entry name

USF2_HUMAN

Accession

Primary (citable) accession number: Q15853
Secondary accession number(s): O00671

Q6FI33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents