Q15849 (UT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urea transporter 2

Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney

Gene names

Name:	SLC14A2
Synonyms:	HUT2, UT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Ref.1 Ref.2
Subunit structure	Isoform 1 interacts with SNAPIN which may be important for recruitment to the plasma membrane. Ref.6
Subcellular location	Apical cell membrane; Multi-pass membrane protein.
Tissue specificity	Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Ref.1 Ref.2
Induction	Isoform 1 is induced by low protein in the diet. Isoform 2 is induced by dehydration.
Sequence similarities	Belongs to the urea transporter family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement Ref.1. Source: ProtInc plasma membrane Traceable author statement. Source: Reactome
Molecular_function	urea transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SNAPIN	O95295	5	EBI-1633392,EBI-296723

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15849-1)

Also known as: UT-A1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15849-2)

Also known as: UT-A2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-523: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Urea transporter 2		PRO_0000065739
Regions
	Transmembrane	151 – 170	20	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Transmembrane	204 – 224	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	272 – 291	20	Helical; Potential
	Transmembrane	302 – 322	21	Helical; Potential
	Transmembrane	346 – 366	21	Helical; Potential
	Transmembrane	370 – 390	21	Helical; Potential
	Transmembrane	392 – 412	21	Helical; Potential
	Transmembrane	600 – 620	21	Helical; Potential
	Transmembrane	638 – 658	21	Helical; Potential
	Transmembrane	666 – 686	21	Helical; Potential
	Transmembrane	695 – 715	21	Helical; Potential
	Transmembrane	764 – 784	21	Helical; Potential
	Transmembrane	803 – 823	21	Helical; Potential
	Transmembrane	832 – 852	21	Helical; Potential
	Transmembrane	854 – 874	21	Helical; Potential
Amino acid modifications
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	54	1	Phosphoserine By similarity
	Modified residue	55	1	Phosphoserine By similarity
	Glycosylation	733	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 523	523	Missing in isoform 2.		VSP_031171
	Natural variant	37	1	T → I. Corresponds to variant rs34461862 [ dbSNP | Ensembl ].		VAR_057016
	Natural variant	132	1	I → V. Ref.2 Ref.3 Ref.5 Corresponds to variant rs1484873 [ dbSNP | Ensembl ].		VAR_060255
	Natural variant	443	1	G → S. Corresponds to variant rs35245152 [ dbSNP | Ensembl ].		VAR_057017
	Natural variant	510	1	R → Q. Ref.2 Ref.3 Ref.5 Corresponds to variant rs9960464 [ dbSNP | Ensembl ].		VAR_060256
	Natural variant	750	1	V → I. Corresponds to variant rs1123617 [ dbSNP | Ensembl ].		VAR_057018
	Natural variant	880	1	A → T. Ref.5 Corresponds to variant rs3745009 [ dbSNP | Ensembl ].		VAR_038690
Experimental info
	Sequence conflict	195	1	V → A in AAL08485. Ref.2
	Sequence conflict	515	1	T → I in BAF85111. Ref.3
	Sequence conflict	723	1	T → A in AAL08485. Ref.2
	Sequence conflict	905	1	N → D in BAF85111. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (UT-A1) [UniParc]. Last modified March 23, 2010. Version 3. Checksum: 23BF379DC0767619	FASTA	920	101,209
	10 20 30 40 50 60 MSDPHSSPLL PEPLSSRYKL YEAEFTSPSW PSTSPDTHPA LPLLEMPEEK DLRSSNEDSH 70 80 90 100 110 120 IVKIEKLNER SKRKDDGVAH RDSAGQRCIC LSKAVGYLTG DMKEYRIWLK DKHLALQFID 130 140 150 160 170 180 WVLRGTAQVM FINNPLSGLI IFIGLLIQNP WWTITGGLGT VVSTLTALAL GQDRSAIASG 190 200 210 220 230 240 LHGYNGMLVG LLMAVFSEKL DYYWWLLFPV TFTAMSCPVL SSALNSIFSK WDLPVFTLPF 250 260 270 280 290 300 NIAVTLYLAA TGHYNLFFPT TLVEPVSSVP NITWTEMEMP LLLQAIPVGV GQVYGCDNPW 310 320 330 340 350 360 TGGVFLVALF ISSPLICLHA AIGSIVGLLA ALSVATPFET IYTGLWSYNC VLSCIAIGGM 370 380 390 400 410 420 FYALTWQTHL LALICALFCA YMEAAISNIM SVVGVPPGTW AFCLATIIFL LLTTNNPAIF 430 440 450 460 470 480 RLPLSKVTYP EANRIYYLTV KSGEEEKAPS GGGGEHPPTA GPKVEEGSEA VLSKHRSVFH 490 500 510 520 530 540 IEWSSIRRRS KVFGKGEHQE RQNKDPFPYR YRKPTVELLD LDTMEESSEI KVETNISKTS 550 560 570 580 590 600 WIRSSMAASG KRVSKALSYI TGEMKECGEG LKDKSPVFQF FDWVLRGTSQ VMFVNNPLSG 610 620 630 640 650 660 ILIILGLFIQ NPWWAISGCL GTIMSTLTAL ILSQDKSAIA AGFHGYNGVL VGLLMAVFSD 670 680 690 700 710 720 KGDYYWWLLL PVIIMSMSCP ILSSALGTIF SKWDLPVFTL PFNITVTLYL AATGHYNLFF 730 740 750 760 770 780 PTTLLQPASA MPNITWSEVQ VPLLLRAIPV GIGQVYGCDN PWTGGIFLIA LFISSPLICL 790 800 810 820 830 840 HAAIGSTMGM LAALTIATPF DSIYFGLCGF NSTLACIAIG GMFYVITWQT HLLAIACALF 850 860 870 880 890 900 AAYLGAALAN MLSVFGLPPC TWPFCLSALT FLLLTTNNPA IYKLPLSKVT YPEANRIYYL 910 920 SQERNRRASI ITKYQAYDVS
	Isoform 2 (UT-A2) [UniParc]. Checksum: 310AD64B1DFA9623	FASTA	397	43,395
	10 20 30 40 50 60 MEESSEIKVE TNISKTSWIR SSMAASGKRV SKALSYITGE MKECGEGLKD KSPVFQFFDW 70 80 90 100 110 120 VLRGTSQVMF VNNPLSGILI ILGLFIQNPW WAISGCLGTI MSTLTALILS QDKSAIAAGF 130 140 150 160 170 180 HGYNGVLVGL LMAVFSDKGD YYWWLLLPVI IMSMSCPILS SALGTIFSKW DLPVFTLPFN 190 200 210 220 230 240 ITVTLYLAAT GHYNLFFPTT LLQPASAMPN ITWSEVQVPL LLRAIPVGIG QVYGCDNPWT 250 260 270 280 290 300 GGIFLIALFI SSPLICLHAA IGSTMGMLAA LTIATPFDSI YFGLCGFNST LACIAIGGMF 310 320 330 340 350 360 YVITWQTHLL AIACALFAAY LGAALANMLS VFGLPPCTWP FCLSALTFLL LTTNNPAIYK 370 380 390 LPLSKVTYPE ANRIYYLSQE RNRRASIITK YQAYDVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a new urea transporter in the human kidney." Olives B., Sonia M., Mattei M.-G., Matassi G., Rousselet G., Ripoche P., Cartron J.-P., Bailly P. FEBS Lett. 386:156-160(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"Cloning and characterization of the human urea transporter UT-A1 and mapping of the human Slc14a2 gene." Bagnasco S.M., Peng T., Janech M.G., Karakashian A., Sands J.M. Am. J. Physiol. 281:F400-F406(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANTS VAL-132 AND GLN-510. Tissue: Kidney.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-132 AND GLN-510. Tissue: Testis.
[4]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-132; GLN-510 AND THR-880.
[6]	"The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein." Mistry A.C., Mallick R., Froehlich O., Klein J.D., Rehm A., Chen G., Sands J.M. J. Biol. Chem. 282:30097-30106(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNAPIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96969 mRNA. Translation: CAA65657.1. AF349446 mRNA. Translation: AAL08485.1. AK292422 mRNA. Translation: BAF85111.1. AC023421 Genomic DNA. No translation available. BC110446 mRNA. Translation: AAI10447.1.
IPI	IPI00177820. IPI00885172.
PIR	S71339.
RefSeq	NP_001229621.1. NM_001242692.1. NP_009094.3. NM_007163.3.
UniGene	Hs.710927.
3D structure databases
ProteinModelPortal	Q15849.
SMR	Q15849. Positions 96-438, 559-900.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15849. 2 interactions.
STRING	9606.ENSP00000255226.
PTM databases
PhosphoSite	Q15849.
Polymorphism databases
DMDM	292495053.
Proteomic databases
PaxDb	Q15849.
PRIDE	Q15849.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000255226; ENSP00000255226; ENSG00000132874. ENST00000586448; ENSP00000465953; ENSG00000132874. ENST00000589658; ENSP00000465349; ENSG00000132874.
GeneID	8170.
KEGG	hsa:8170.
UCSC	uc002lbe.3. human.
Organism-specific databases
CTD	8170.
GeneCards	GC18P043194.
H-InvDB	HIX0014422.
HGNC	HGNC:10919. SLC14A2.
HPA	HPA050069.
MIM	601611. gene.
neXtProt	NX_Q15849.
PharmGKB	PA35811.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4413.
HOGENOM	HOG000168437.
HOVERGEN	HBG073255.
InParanoid	Q15849.
KO	K08716.
OMA	GTWAFCL.
OrthoDB	EOG48D0VG.
PhylomeDB	Q15849.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_20679. Amine compound SLC transporters.
Gene expression databases
ArrayExpress	Q15849.
Bgee	Q15849.
CleanEx	HS_SLC14A2.
Genevestigator	Q15849.
GermOnline	ENSG00000132874. Homo sapiens.
Family and domain databases
InterPro	IPR004937. Urea_transporter. [Graphical view]
PANTHER	PTHR10464. PTHR10464. 1 hit.
Pfam	PF03253. UT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC14A2. human.
GenomeRNAi	8170.
NextBio	30845.
SOURCE	Search...

Entry information

Entry name

UT2_HUMAN

Accession

Primary (citable) accession number: Q15849
Secondary accession number(s): A8K8Q7, Q2TBD6, Q96PH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.