ID   ADIRF_HUMAN             Reviewed;          76 AA.
AC   Q15847;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Adipogenesis regulatory factor;
DE   AltName: Full=Adipogenesis factor rich in obesity;
DE   AltName: Full=Adipose most abundant gene transcript 2 protein;
DE   AltName: Full=Adipose-specific protein 2;
DE            Short=apM-2;
GN   Name=ADIRF; Synonyms=AFRO, APM2, C10orf116;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Adipose tissue;
RA   Maeda K.;
RL   Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164054; DOI=10.1038/nature02462;
RA   Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA   Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA   Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA   Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA   Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA   Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA   Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA   Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA   Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA   Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA   Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA   Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA   McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA   Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA   Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA   Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA   Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA   Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA   Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA   Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA   Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 10.";
RL   Nature 429:375-381(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   TISSUE SPECIFICITY.
RA   Richardson M.;
RL   Submitted (SEP-2004) to UniProtKB.
RN   [5]
RP   FUNCTION IN RESISTANCE TO CISPLATIN, AND INDUCTION.
RX   PubMed=19444912; DOI=10.1002/ijc.24465;
RA   Scott B.J., Qutob S., Liu Q.Y., Ng C.E.;
RT   "APM2 is a novel mediator of cisplatin resistance in a variety of cancer
RT   cell types regardless of p53 or MMR status.";
RL   Int. J. Cancer 125:1193-1204(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND TISSUE SPECIFICITY.
RX   PubMed=23239344; DOI=10.1007/s10863-012-9492-6;
RA   Ni Y., Ji C., Wang B., Qiu J., Wang J., Guo X.;
RT   "A Novel pro-adipogenesis factor abundant in adipose tissues and over-
RT   expressed in obesity acts upstream of PPARgamma and C/EBPalpha.";
RL   J. Bioenerg. Biomembr. 45:219-228(2013).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: Plays a role in fat cell development; promotes adipogenic
CC       differentiation and stimulates transcription initiation of master
CC       adipogenesis factors like PPARG and CEBPA at early stages of
CC       preadipocyte differentiation. Its overexpression confers resistance to
CC       the anticancer chemotherapeutic drug cisplatin.
CC       {ECO:0000269|PubMed:19444912, ECO:0000269|PubMed:23239344}.
CC   -!- INTERACTION:
CC       Q15847; Q14116: IL18; NbExp=3; IntAct=EBI-7162516, EBI-3910835;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23239344}.
CC   -!- TISSUE SPECIFICITY: Expressed in adipose tissue (at protein level).
CC       Highly expressed in omental and subcutaneous adipose tissues. Expressed
CC       in heart, cornea, liver, kidney and spleen.
CC       {ECO:0000269|PubMed:23239344, ECO:0000269|Ref.4}.
CC   -!- INDUCTION: Up-regulated during pre-adipocyte differentiation. Up-
CC       regulated following DNA damage induced by UV irradiation.
CC       {ECO:0000269|PubMed:19444912, ECO:0000269|PubMed:23239344}.
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DR   EMBL; D45370; BAA08226.1; -; mRNA.
DR   EMBL; AL136982; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC004471; AAH04471.1; -; mRNA.
DR   CCDS; CCDS7381.1; -.
DR   RefSeq; NP_006820.1; NM_006829.2.
DR   AlphaFoldDB; Q15847; -.
DR   SMR; Q15847; -.
DR   BioGRID; 116171; 9.
DR   IntAct; Q15847; 4.
DR   MINT; Q15847; -.
DR   STRING; 9606.ENSP00000361083; -.
DR   GlyCosmos; Q15847; 1 site, 1 glycan.
DR   GlyGen; Q15847; 2 sites, 2 O-linked glycans (2 sites).
DR   iPTMnet; Q15847; -.
DR   PhosphoSitePlus; Q15847; -.
DR   BioMuta; ADIRF; -.
DR   DMDM; 8927989; -.
DR   EPD; Q15847; -.
DR   jPOST; Q15847; -.
DR   MassIVE; Q15847; -.
DR   PaxDb; 9606-ENSP00000361083; -.
DR   PeptideAtlas; Q15847; -.
DR   ProteomicsDB; 60790; -.
DR   Pumba; Q15847; -.
DR   Antibodypedia; 56434; 57 antibodies from 14 providers.
DR   DNASU; 10974; -.
DR   Ensembl; ENST00000372013.8; ENSP00000361083.3; ENSG00000148671.14.
DR   Ensembl; ENST00000561504.1; ENSP00000475582.1; ENSG00000148671.14.
DR   GeneID; 10974; -.
DR   KEGG; hsa:10974; -.
DR   MANE-Select; ENST00000372013.8; ENSP00000361083.3; NM_006829.3; NP_006820.1.
DR   AGR; HGNC:24043; -.
DR   CTD; 10974; -.
DR   DisGeNET; 10974; -.
DR   GeneCards; ADIRF; -.
DR   HGNC; HGNC:24043; ADIRF.
DR   HPA; ENSG00000148671; Tissue enhanced (adipose).
DR   neXtProt; NX_Q15847; -.
DR   OpenTargets; ENSG00000148671; -.
DR   PharmGKB; PA134930409; -.
DR   VEuPathDB; HostDB:ENSG00000148671; -.
DR   eggNOG; ENOG502SYWB; Eukaryota.
DR   GeneTree; ENSGT00390000017446; -.
DR   HOGENOM; CLU_199186_0_0_1; -.
DR   InParanoid; Q15847; -.
DR   OMA; XDQATET; -.
DR   OrthoDB; 4641953at2759; -.
DR   PhylomeDB; Q15847; -.
DR   TreeFam; TF341403; -.
DR   PathwayCommons; Q15847; -.
DR   Reactome; R-HSA-381340; Transcriptional regulation of white adipocyte differentiation.
DR   SignaLink; Q15847; -.
DR   BioGRID-ORCS; 10974; 8 hits in 1148 CRISPR screens.
DR   ChiTaRS; ADIRF; human.
DR   GenomeRNAi; 10974; -.
DR   Pharos; Q15847; Tbio.
DR   PRO; PR:Q15847; -.
DR   Proteomes; UP000005640; Chromosome 10.
DR   RNAct; Q15847; Protein.
DR   Bgee; ENSG00000148671; Expressed in popliteal artery and 200 other cell types or tissues.
DR   ExpressionAtlas; Q15847; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0045600; P:positive regulation of fat cell differentiation; IDA:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   InterPro; IPR034450; ADIRF.
DR   PANTHER; PTHR39227; ADIPOGENESIS REGULATORY FACTOR; 1.
DR   PANTHER; PTHR39227:SF1; ADIPOGENESIS REGULATORY FACTOR; 1.
DR   Genevisible; Q15847; HS.
PE   1: Evidence at protein level;
KW   Differentiation; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..76
FT                   /note="Adipogenesis regulatory factor"
FT                   /id="PRO_0000064637"
SQ   SEQUENCE   76 AA;  7855 MW;  C95B41F13C4BA7B1 CRC64;
     MASKGLQDLK QQVEGTAQEA VSAAGAAAQQ VVDQATEAGQ KAMDQLAKTT QETIDKTANQ
     ASDTFSGIGK KFGLLK
//