ID KCNJ8_HUMAN Reviewed; 424 AA. AC Q15842; O00657; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 24-JAN-2024, entry version 207. DE RecName: Full=ATP-sensitive inward rectifier potassium channel 8; DE AltName: Full=Inward rectifier K(+) channel Kir6.1; DE AltName: Full=Potassium channel, inwardly rectifying subfamily J member 8; DE AltName: Full=uKATP-1; GN Name=KCNJ8; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. RC TISSUE=Lung, and Placenta; RX PubMed=8595887; DOI=10.1006/geno.1995.0018; RA Inagaki N., Inazawa J., Seino S.; RT "cDNA sequence, gene structure, and chromosomal localization of the human RT ATP-sensitive potassium channel, uKATP-1, gene (KCNJ8)."; RL Genomics 30:102-104(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Brain; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP TISSUE SPECIFICITY. RX PubMed=9573340; DOI=10.1016/s0378-1119(98)00086-9; RA Erginel-Unaltuna N., Yang W.P., Blanar M.A.; RT "Genomic organization and expression of KCNJ8/Kir6.1, a gene encoding a RT subunit of an ATP-sensitive potassium channel."; RL Gene 211:71-78(1998). RN [4] RP REVIEW ON J-WAVE SYNDROMES. RX PubMed=20153265; DOI=10.1016/j.hrthm.2009.12.006; RA Antzelevitch C., Yan G.X.; RT "J wave syndromes."; RL Heart Rhythm 7:549-558(2010). RN [5] RP POSSIBLE INVOLVEMENT IN SUSCEPTIBILITY TO J-WAVE SYNDROMES, VARIANT RP LEU-422, AND CHARACTERIZATION OF VARIANT LEU-422. RX PubMed=20558321; DOI=10.1016/j.hrthm.2010.06.016; RA Medeiros-Domingo A., Tan B.H., Crotti L., Tester D.J., Eckhardt L., RA Cuoretti A., Kroboth S.L., Song C., Zhou Q., Kopp D., Schwartz P.J., RA Makielski J.C., Ackerman M.J.; RT "Gain-of-function mutation S422L in the KCNJ8-encoded cardiac K(ATP) RT channel Kir6.1 as a pathogenic substrate for J-wave syndromes."; RL Heart Rhythm 7:1466-1471(2010). RN [6] RP POSSIBLE INVOLVEMENT IN HTOCD, VARIANT HTOCD SER-176, AND CHARACTERIZATION RP OF VARIANT HTOCD SER-176. RX PubMed=24700710; DOI=10.1002/humu.22555; RA Cooper P.E., Reutter H., Woelfle J., Engels H., Grange D.K., RA van Haaften G., van Bon B.W., Hoischen A., Nichols C.G.; RT "Cantu syndrome resulting from activating mutation in the KCNJ8 gene."; RL Hum. Mutat. 35:809-813(2014). RN [7] RP FUNCTION, CHARACTERIZATION OF VARIANT HTOCD MET-65, AND MUTAGENESIS OF RP VAL-65. RX PubMed=28842488; DOI=10.1074/jbc.m117.804971; RA Cooper P.E., McClenaghan C., Chen X., Stary-Weinzinger A., Nichols C.G.; RT "Conserved functional consequences of disease-associated mutations in the RT slide-helix of Kir6.1 and Kir6.2 subunits of the ATP-sensitive potassium RT channel."; RL J. Biol. Chem. 292:17387-17398(2017). RN [8] RP VARIANTS SIDS GLU-332 DEL AND ILE-346, AND CHARACTERIZATION OF VARIANTS RP SIDS GLU-332 DEL AND ILE-346. RX PubMed=21836131; DOI=10.1161/circgenetics.111.960195; RA Tester D.J., Tan B.H., Medeiros-Domingo A., Song C., Makielski J.C., RA Ackerman M.J.; RT "Loss-of-function mutations in the KCNJ8-encoded Kir6.1 KATP channel and RT sudden infant death syndrome."; RL Circ. Cardiovasc. Genet. 4:510-515(2011). RN [9] RP VARIANT HTOCD MET-65. RX PubMed=24176758; DOI=10.1016/j.ejmg.2013.09.009; RA Brownstein C.A., Towne M.C., Luquette L.J., Harris D.J., Marinakis N.S., RA Meinecke P., Kutsche K., Campeau P.M., Yu T.W., Margulies D.M., RA Agrawal P.B., Beggs A.H.; RT "Mutation of KCNJ8 in a patient with Cantu syndrome with unique vascular RT abnormalities - support for the role of K(ATP) channels in this RT condition."; RL Eur. J. Med. Genet. 56:678-682(2013). CC -!- FUNCTION: This potassium channel is controlled by G proteins. Inward CC rectifier potassium channels are characterized by a greater tendency to CC allow potassium to flow into the cell rather than out of it. Their CC voltage dependence is regulated by the concentration of extracellular CC potassium; as external potassium is raised, the voltage range of the CC channel opening shifts to more positive voltages. The inward CC rectification is mainly due to the blockage of outward current by CC internal magnesium. Can be blocked by external barium (By similarity). CC {ECO:0000250|UniProtKB:Q63664, ECO:0000269|PubMed:28842488}. CC -!- INTERACTION: CC Q15842; P50402: EMD; NbExp=3; IntAct=EBI-17440235, EBI-489887; CC Q15842; Q9NV12: TMEM140; NbExp=3; IntAct=EBI-17440235, EBI-2844246; CC Q15842; Q969K7: TMEM54; NbExp=3; IntAct=EBI-17440235, EBI-3922833; CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein CC {ECO:0000305}. CC -!- TISSUE SPECIFICITY: Predominantly detected in fetal and adult heart. CC {ECO:0000269|PubMed:9573340}. CC -!- DISEASE: Note=Defects in KCNJ8 may be associated with susceptibility to CC J-wave syndromes, a group of heart disorders characterized by early CC repolarization events as indicated by abnormal J-wave manifestation on CC electrocardiogram (ECG). The J point denotes the junction of the QRS CC complex and the ST segment on the ECG, marking the end of CC depolarization and the beginning of repolarization. An abnormal J wave CC is a deflection with a dome or hump morphology immediately following CC the QRS complex of the surface ECG. Examples of J-wave disorders are CC arrhythmias associated with an early repolarization pattern in the CC inferior or mid to lateral precordial leads, Brugada syndrome, some CC cases of idiopathic ventricular fibrillation (VF) with an early CC repolarization pattern in the inferior, inferolateral or global leads, CC as well as arrhythmias associated with hypothermia. CC {ECO:0000269|PubMed:20558321}. CC -!- DISEASE: Sudden infant death syndrome (SIDS) [MIM:272120]: SIDS is the CC sudden death of an infant younger than 1 year that remains unexplained CC after a thorough case investigation, including performance of a CC complete autopsy, examination of the death scene, and review of CC clinical history. Pathophysiologic mechanisms for SIDS may include CC respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory CC instability, and inborn errors of metabolism, but definitive pathogenic CC mechanisms precipitating an infant sudden death remain elusive. CC {ECO:0000269|PubMed:21836131}. Note=Disease susceptibility is CC associated with variants affecting the gene represented in this entry. CC -!- DISEASE: Hypertrichotic osteochondrodysplasia (HTOCD) [MIM:239850]: A CC rare disorder characterized by congenital hypertrichosis, neonatal CC macrosomia, a distinct osteochondrodysplasia, and cardiomegaly. The CC hypertrichosis leads to thick scalp hair, which extends onto the CC forehead, and a general increase in body hair. In addition, CC macrocephaly and coarse facial features, including a broad nasal CC bridge, epicanthal folds, a wide mouth, and full lips, can be CC suggestive of a storage disorder. About half of affected individuals CC are macrosomic and edematous at birth, whereas in childhood they CC usually have a muscular appearance with little subcutaneous fat. CC Thickened calvarium, narrow thorax, wide ribs, flattened or ovoid CC vertebral bodies, coxa valga, osteopenia, enlarged medullary canals, CC and metaphyseal widening of long bones have been reported. Cardiac CC manifestations such as patent ductus arteriosus, ventricular CC hypertrophy, pulmonary hypertension, and pericardial effusions are CC present in approximately 80% of cases. Motor development is usually CC delayed due to hypotonia. Most patients have a mild speech delay, and a CC small percentage have learning difficulties or intellectual disability. CC {ECO:0000269|PubMed:24176758, ECO:0000269|PubMed:24700710, CC ECO:0000269|PubMed:28842488}. Note=The disease may be caused by CC variants affecting distinct genetic loci, including the gene CC represented in this entry. CC -!- SIMILARITY: Belongs to the inward rectifier-type potassium channel CC (TC 1.A.2.1) family. KCNJ8 subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D50312; BAA08851.1; -; mRNA. DR EMBL; D50315; BAA08852.1; -; Genomic_DNA. DR EMBL; BC000544; AAH00544.1; -; mRNA. DR CCDS; CCDS8692.1; -. DR RefSeq; NP_004973.1; NM_004982.3. DR RefSeq; XP_005253415.1; XM_005253358.4. DR RefSeq; XP_016874772.1; XM_017019283.1. DR RefSeq; XP_016874773.1; XM_017019284.1. DR AlphaFoldDB; Q15842; -. DR SMR; Q15842; -. DR BioGRID; 109966; 42. DR ELM; Q15842; -. DR IntAct; Q15842; 16. DR STRING; 9606.ENSP00000499300; -. DR ChEMBL; CHEMBL4770; -. DR DrugBank; DB11148; Butamben. DR DrugBank; DB01251; Gliquidone. DR DrugBank; DB01289; Glisoxepide. DR DrugBank; DB01016; Glyburide. DR DrugBank; DB00922; Levosimendan. DR DrugBank; DB00914; Phenformin. DR DrugBank; DB00867; Ritodrine. DR DrugBank; DB01154; Thiamylal. DR DrugBank; DB01392; Yohimbine. DR DrugCentral; Q15842; -. DR TCDB; 1.A.2.1.13; the inward rectifier k(+) channel (irk-c) family. DR iPTMnet; Q15842; -. DR PhosphoSitePlus; Q15842; -. DR BioMuta; KCNJ8; -. DR DMDM; 2493600; -. DR jPOST; Q15842; -. DR MassIVE; Q15842; -. DR PaxDb; 9606-ENSP00000240662; -. DR PeptideAtlas; Q15842; -. DR ProteomicsDB; 60787; -. DR Antibodypedia; 4014; 279 antibodies from 31 providers. DR DNASU; 3764; -. DR Ensembl; ENST00000240662.3; ENSP00000240662.2; ENSG00000121361.5. DR Ensembl; ENST00000665145.1; ENSP00000499300.1; ENSG00000121361.5. DR Ensembl; ENST00000667884.1; ENSP00000499462.1; ENSG00000121361.5. DR GeneID; 3764; -. DR KEGG; hsa:3764; -. DR MANE-Select; ENST00000240662.3; ENSP00000240662.2; NM_004982.4; NP_004973.1. DR UCSC; uc001rff.4; human. DR AGR; HGNC:6269; -. DR CTD; 3764; -. DR DisGeNET; 3764; -. DR GeneCards; KCNJ8; -. DR GeneReviews; KCNJ8; -. DR HGNC; HGNC:6269; KCNJ8. DR HPA; ENSG00000121361; Tissue enhanced (heart). DR MalaCards; KCNJ8; -. DR MIM; 239850; phenotype. DR MIM; 272120; phenotype. DR MIM; 600935; gene. DR neXtProt; NX_Q15842; -. DR OpenTargets; ENSG00000121361; -. DR Orphanet; 130; Brugada syndrome. DR Orphanet; 1517; Cantu syndrome. DR PharmGKB; PA30050; -. DR VEuPathDB; HostDB:ENSG00000121361; -. DR eggNOG; KOG3827; Eukaryota. DR GeneTree; ENSGT00990000203615; -. DR HOGENOM; CLU_022738_4_0_1; -. DR InParanoid; Q15842; -. DR OMA; ISEEIQW; -. DR OrthoDB; 4126787at2759; -. DR PhylomeDB; Q15842; -. DR TreeFam; TF313676; -. DR PathwayCommons; Q15842; -. DR Reactome; R-HSA-1296025; ATP sensitive Potassium channels. DR SignaLink; Q15842; -. DR SIGNOR; Q15842; -. DR BioGRID-ORCS; 3764; 8 hits in 1161 CRISPR screens. DR GeneWiki; KCNJ8; -. DR GenomeRNAi; 3764; -. DR Pharos; Q15842; Tbio. DR PRO; PR:Q15842; -. DR Proteomes; UP000005640; Chromosome 12. DR RNAct; Q15842; Protein. DR Bgee; ENSG00000121361; Expressed in heart right ventricle and 158 other cell types or tissues. DR ExpressionAtlas; Q15842; baseline and differential. DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl. DR GO; GO:0008282; C:inward rectifying potassium channel; IEA:Ensembl. DR GO; GO:0030016; C:myofibril; IEA:Ensembl. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0031004; C:potassium ion-transporting ATPase complex; ISS:ARUK-UCL. DR GO; GO:0048787; C:presynaptic active zone membrane; IEA:Ensembl. DR GO; GO:0042383; C:sarcolemma; IEA:Ensembl. DR GO; GO:0008076; C:voltage-gated potassium channel complex; TAS:ProtInc. DR GO; GO:0005524; F:ATP binding; ISS:ARUK-UCL. DR GO; GO:0015272; F:ATP-activated inward rectifier potassium channel activity; IDA:BHF-UCL. DR GO; GO:0019829; F:ATPase-coupled monoatomic cation transmembrane transporter activity; ISS:ARUK-UCL. DR GO; GO:0005242; F:inward rectifier potassium channel activity; TAS:ProtInc. DR GO; GO:0017098; F:sulfonylurea receptor binding; IEA:Ensembl. DR GO; GO:0099508; F:voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential; IDA:SynGO. DR GO; GO:1902282; F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization; IMP:BHF-UCL. DR GO; GO:0002250; P:adaptive immune response; IEA:Ensembl. DR GO; GO:0006915; P:apoptotic process; IEA:Ensembl. DR GO; GO:0060922; P:atrioventricular node cell differentiation; IEA:Ensembl. DR GO; GO:0070588; P:calcium ion transmembrane transport; IEA:Ensembl. DR GO; GO:0061762; P:CAMKK-AMPK signaling cascade; IEA:Ensembl. DR GO; GO:0060976; P:coronary vasculature development; IEA:Ensembl. DR GO; GO:0051607; P:defense response to virus; IEA:Ensembl. DR GO; GO:0008340; P:determination of adult lifespan; IEA:Ensembl. DR GO; GO:0030010; P:establishment of cell polarity; IEA:Ensembl. DR GO; GO:0045444; P:fat cell differentiation; IEA:Ensembl. DR GO; GO:0015908; P:fatty acid transport; IEA:Ensembl. DR GO; GO:0048144; P:fibroblast proliferation; IEA:Ensembl. DR GO; GO:0010467; P:gene expression; IEA:Ensembl. DR GO; GO:0061535; P:glutamate secretion, neurotransmission; IEA:Ensembl. DR GO; GO:0003007; P:heart morphogenesis; IEA:Ensembl. DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISS:ARUK-UCL. DR GO; GO:0001822; P:kidney development; IEA:Ensembl. DR GO; GO:0098915; P:membrane repolarization during ventricular cardiac muscle cell action potential; IMP:BHF-UCL. DR GO; GO:0001774; P:microglial cell activation; IEA:Ensembl. DR GO; GO:0050905; P:neuromuscular process; IEA:Ensembl. DR GO; GO:0044546; P:NLRP3 inflammasome complex assembly; IEA:Ensembl. DR GO; GO:0038066; P:p38MAPK cascade; IEA:Ensembl. DR GO; GO:1990573; P:potassium ion import across plasma membrane; IDA:BHF-UCL. DR GO; GO:0071805; P:potassium ion transmembrane transport; NAS:ARUK-UCL. DR GO; GO:0006813; P:potassium ion transport; TAS:ProtInc. DR GO; GO:0009306; P:protein secretion; IEA:Ensembl. DR GO; GO:0150103; P:reactive gliosis; IEA:Ensembl. DR GO; GO:0008217; P:regulation of blood pressure; IEA:Ensembl. DR GO; GO:0002027; P:regulation of heart rate; IEA:Ensembl. DR GO; GO:0034765; P:regulation of monoatomic ion transmembrane transport; IBA:GO_Central. DR GO; GO:0033198; P:response to ATP; IEA:Ensembl. DR GO; GO:0034097; P:response to cytokine; IEA:Ensembl. DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IEA:Ensembl. DR GO; GO:0043330; P:response to exogenous dsRNA; IEA:Ensembl. DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl. DR GO; GO:0032868; P:response to insulin; IEA:Ensembl. DR GO; GO:0002931; P:response to ischemia; IEA:Ensembl. DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl. DR GO; GO:1904638; P:response to resveratrol; IEA:Ensembl. DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl. DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IEA:Ensembl. DR GO; GO:0019226; P:transmission of nerve impulse; IEA:Ensembl. DR GO; GO:0150104; P:transport across blood-brain barrier; NAS:ARUK-UCL. DR GO; GO:0042311; P:vasodilation; IEA:Ensembl. DR GO; GO:0003229; P:ventricular cardiac muscle tissue development; IEA:Ensembl. DR Gene3D; 1.10.287.70; -; 1. DR Gene3D; 2.60.40.1400; G protein-activated inward rectifier potassium channel 1; 1. DR InterPro; IPR014756; Ig_E-set. DR InterPro; IPR041647; IRK_C. DR InterPro; IPR016449; K_chnl_inward-rec_Kir. DR InterPro; IPR003278; K_chnl_inward-rec_Kir6.1. DR InterPro; IPR013518; K_chnl_inward-rec_Kir_cyto. DR InterPro; IPR040445; Kir_TM. DR PANTHER; PTHR11767:SF11; ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8; 1. DR PANTHER; PTHR11767; INWARD RECTIFIER POTASSIUM CHANNEL; 1. DR Pfam; PF01007; IRK; 1. DR Pfam; PF17655; IRK_C; 1. DR PIRSF; PIRSF005465; GIRK_kir; 1. DR PRINTS; PR01331; KIR61CHANNEL. DR PRINTS; PR01320; KIRCHANNEL. DR SUPFAM; SSF81296; E set domains; 1. DR SUPFAM; SSF81324; Voltage-gated potassium channels; 1. DR Genevisible; Q15842; HS. PE 1: Evidence at protein level; KW Disease variant; Ion channel; Ion transport; Membrane; Phosphoprotein; KW Potassium; Potassium transport; Reference proteome; Transmembrane; KW Transmembrane helix; Transport; Voltage-gated channel. FT CHAIN 1..424 FT /note="ATP-sensitive inward rectifier potassium channel 8" FT /id="PRO_0000154947" FT TOPO_DOM 1..69 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT TRANSMEM 70..94 FT /note="Helical; Name=M1" FT /evidence="ECO:0000250" FT TOPO_DOM 95..126 FT /note="Extracellular" FT /evidence="ECO:0000250" FT INTRAMEM 127..138 FT /note="Helical; Pore-forming; Name=H5" FT /evidence="ECO:0000250" FT INTRAMEM 139..145 FT /note="Pore-forming" FT /evidence="ECO:0000250" FT TOPO_DOM 146..154 FT /note="Extracellular" FT /evidence="ECO:0000250" FT TRANSMEM 155..176 FT /note="Helical; Name=M2" FT /evidence="ECO:0000250" FT TOPO_DOM 177..424 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT REGION 375..424 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 140..145 FT /note="Selectivity filter" FT /evidence="ECO:0000250" FT COMPBIAS 390..424 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT SITE 170 FT /note="Role in the control of polyamine-mediated channel FT gating and in the blocking by intracellular magnesium" FT /evidence="ECO:0000250" FT MOD_RES 6 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P97794" FT VARIANT 65 FT /note="V -> M (in HTOCD; uncertain significance; displays FT gain of function; increased open state stability, reduced FT sensitivity to ATP inhibition and increased channel FT activity; almost completely abolishes high affinity FT sensitivity to glibenclamide, an inhibitor of ATP-sensitive FT potassium channels; dbSNP:rs606231263)" FT /evidence="ECO:0000269|PubMed:24176758, FT ECO:0000269|PubMed:28842488" FT /id="VAR_079518" FT VARIANT 176 FT /note="C -> S (in HTOCD; uncertain significance; displays FT gain of function; displays reduced ATP sensitivity; FT dbSNP:rs606231264)" FT /evidence="ECO:0000269|PubMed:24700710" FT /id="VAR_075226" FT VARIANT 332 FT /note="Missing (in SIDS; the mutant channel displays FT reduced potassium currents compared to wild type)" FT /evidence="ECO:0000269|PubMed:21836131" FT /id="VAR_065878" FT VARIANT 334 FT /note="V -> A (in dbSNP:rs34811413)" FT /id="VAR_049670" FT VARIANT 346 FT /note="V -> I (in SIDS; the mutant channel displays reduced FT potassium currents compared to wild type; FT dbSNP:rs147316959)" FT /evidence="ECO:0000269|PubMed:21836131" FT /id="VAR_065879" FT VARIANT 422 FT /note="S -> L (found in Brugada syndrome and other J-wave FT syndromes; uncertain significance; the mutant channel FT displays an increase in glibenclamide-sensitive potassium FT currents compared to wild type; dbSNP:rs72554071)" FT /evidence="ECO:0000269|PubMed:20558321" FT /id="VAR_065225" FT MUTAGEN 65 FT /note="V->L: No effect on channel activity." FT /evidence="ECO:0000269|PubMed:28842488" SQ SEQUENCE 424 AA; 47968 MW; 973EAA5900C6447C CRC64; MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI REQGRFLQDI FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD IYAYMEKSGM EKSGLESTVC VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE ECPLAITVLI LQNIVGLIIN AVMLGCIFMK TAQAHRRAET LIFSRHAVIA VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV VPIHQLDIPV DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLANQDL EVIVILEGVV ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVKVAA PRCSARELDE KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRNNSIRR NNSSLMVPKV QFMTPEGNQN TSES //