Reviewed, UniProtKB/Swiss-Prot Q15836 (VAMP3_HUMAN)

Names and origin

Protein names

Recommended name:
    Vesicle-associated membrane protein 3
      Short name=VAMP-3
Alternative name(s):
    Synaptobrevin-3
    Cellubrevin
      Short name=CEB

Gene names

Name:	VAMP3
Synonyms:	SYB3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Trafficking protein from a constitutively recycling pathway By similarity.
Subcellular location	Membrane; Single-pass type IV membrane proteinProbable. Cell junction › synapse › synaptosomeBy similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	membrane fusion Ref.1 Traceable author statement. Source: ProtInc protein complex assembly Ref.1 Traceable author statement. Source: ProtInc vesicle docking during exocytosis Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Ref.1 Traceable author statement. Source: ProtInc synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 100	99	Vesicle-associated membrane protein 3		PRO_0000206728
Regions
	Topological domain	2 – 77	76	Cytoplasmic Potential
	Transmembrane	78 – 98	21	Anchor for type IV membrane protein Potential
	Topological domain	99 – 100	2	Vesicular Potential
	Domain	14 – 74	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	58	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q15836-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 347E9163157AFF42	FASTA	100	11,309
	10 20 30 40 50 60 MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF 70 80 90 100 ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." Bernstein A.M., Whiteheart S.W. Blood 93:571-579(1999) [PubMed: 9885218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Urinary bladder.
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U64520 mRNA. Translation: AAB05814.1. BT007327 mRNA. Translation: AAP35991.1. Z98884 Genomic DNA. Translation: CAB63146.1. BC003570 mRNA. Translation: AAH03570.1. BC007050 mRNA. Translation: AAH07050.1.
RefSeq	NP_004772.1.
UniGene	Hs.66708
3D structure databases
HSSP	HSSP built from PDB template 1F83 based on UniProtKB P19065.
SMR	Q15836. Positions 11-78.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15836.
PTM databases
PhosphoSite	Q15836.
Genome annotation databases
Ensembl	ENSG00000049245. Homo sapiens. [Contig view]
GeneID	9341.
KEGG	hsa:9341.
Organism-specific databases
H-InvDB	HIX0000093.
HGNC	HGNC:12644. VAMP3.
MIM	603657. gene.
PharmGKB	PA37268.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q15836.
HOVERGEN	Q15836.
Gene expression databases
ArrayExpress	Q15836.
CleanEx	HS_VAMP3.
GermOnline	ENSG00000049245. Homo sapiens.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
ProDom	PD001229. Synaptobrevin. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	34987.
SOURCE	Search...

Entry information

Entry name

VAMP3_HUMAN

Accession

Primary (citable) accession number: Q15836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)