Q15836 (VAMP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 3
Short name=VAMP-3

Alternative name(s):
Cellubrevin
Short name=CEB
Synaptobrevin-3

Gene names

Name:	VAMP3
Synonyms:	SYB3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Ref.6
Subunit structure	Interacts with BVES (via the C-terminus cytoplasmic tail) By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein Probable. Cell junction › synapse › synaptosome By similarity.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell junction Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane protein transport Inferred from electronic annotation. Source: Compara calcium ion-dependent exocytosis Inferred from electronic annotation. Source: Compara cellular membrane fusion Traceable author statement Ref.1. Source: ProtInc positive regulation of receptor recycling Inferred from sequence or structural similarity. Source: UniProtKB protein complex assembly Traceable author statement Ref.1. Source: ProtInc retrograde transport, endosome to Golgi Inferred from direct assay Ref.6. Source: UniProtKB substrate adhesion-dependent cell spreading Inferred from sequence or structural similarity. Source: UniProtKB vesicle docking involved in exocytosis Traceable author statement Ref.1. Source: ProtInc
Cellular_component	SNARE complex Inferred from electronic annotation. Source: Compara apical plasma membrane Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW clathrin-coated vesicle Inferred from direct assay PubMed 19478182. Source: UniProtKB clathrin-coated vesicle membrane Inferred from electronic annotation. Source: Compara integral to membrane Traceable author statement Ref.1. Source: ProtInc neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB recycling endosome Inferred from direct assay PubMed 19061864. Source: MGI secretory granule Inferred from electronic annotation. Source: Compara synapse Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Picalm	O55012	2	EBI-722343,EBI-915601	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 100	99	Vesicle-associated membrane protein 3		PRO_0000206728
Regions
	Topological domain	2 – 77	76	Cytoplasmic Potential
	Transmembrane	78 – 98	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	99 – 100	2	Vesicular Potential
	Domain	14 – 74	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	44	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15836 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 347E9163157AFF42	FASTA	100	11,309
	10 20 30 40 50 60 MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF 70 80 90 100 ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." Bernstein A.M., Whiteheart S.W. Blood 93:571-579(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Urinary bladder.
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet.
[6]	"A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells." Ganley I.G., Espinosa E., Pfeffer S.R. J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U64520 mRNA. Translation: AAB05814.1. BT007327 mRNA. Translation: AAP35991.1. Z98884 Genomic DNA. Translation: CAB63146.1. BC003570 mRNA. Translation: AAH03570.1. BC007050 mRNA. Translation: AAH07050.1.
IPI	IPI00549343.
RefSeq	NP_004772.1. NM_004781.3.
UniGene	Hs.66708.
3D structure databases
ProteinModelPortal	Q15836.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15836. 6 interactions.
STRING	9606.ENSP00000054666.
PTM databases
PhosphoSite	Q15836.
Polymorphism databases
DMDM	2501082.
Proteomic databases
PaxDb	Q15836.
PRIDE	Q15836.
Protocols and materials databases
DNASU	9341.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000054666; ENSP00000054666; ENSG00000049245.
GeneID	9341.
KEGG	hsa:9341.
UCSC	uc001aol.3. human.
Organism-specific databases
CTD	9341.
GeneCards	GC01P007765.
HGNC	HGNC:12644. VAMP3.
MIM	603657. gene.
neXtProt	NX_Q15836.
PharmGKB	PA37268.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG266115.
HOGENOM	HOG000042711.
HOVERGEN	HBG006675.
InParanoid	Q15836.
KO	K13505.
OMA	MWAIGIV.
OrthoDB	EOG43JC6B.
PhylomeDB	Q15836.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6_traffickingpathway. Arf6 trafficking events.
Gene expression databases
ArrayExpress	Q15836.
Bgee	Q15836.
CleanEx	HS_VAMP3.
Genevestigator	Q15836.
GermOnline	ENSG00000049245. Homo sapiens.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	VAMP3. human.
GenomeRNAi	9341.
NextBio	34987.
PMAP-CutDB	Q15836.
SOURCE	Search...

Entry information

Entry name

VAMP3_HUMAN

Accession

Primary (citable) accession number: Q15836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.