Q15836 (VAMP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 3
Short name=VAMP-3

Alternative name(s):
Cellubrevin
Short name=CEB
Synaptobrevin-3

Gene names

Name:	VAMP3
Synonyms:	SYB3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Ref.7
Subunit structure	Interacts with BVES (via the C-terminus cytoplasmic tail) By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein Probable. Cell junction › synapse › synaptosome By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell junction Membrane Synapse Synaptosome
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular membrane fusion Traceable author statement. Source: ProtInc positive regulation of receptor recycling Inferred from sequence or structural similarity. Source: UniProtKB protein complex assembly Traceable author statement. Source: ProtInc protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from direct assay Ref.7. Source: UniProtKB substrate adhesion-dependent cell spreading Inferred from sequence or structural similarity. Source: UniProtKB vesicle docking involved in exocytosis Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Traceable author statement. Source: ProtInc recycling endosome Inferred from direct assay. Source: MGI synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 100	99	Vesicle-associated membrane protein 3		PRO_0000206728
Regions
	Topological domain	2 – 77	76	Cytoplasmic Potential
	Transmembrane	78 – 98	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	99 – 100	2	Vesicular Potential
	Domain	14 – 74	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	44	1	Phosphoserine By similarity
	Modified residue	58	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q15836 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 347E9163157AFF42	FASTA	100	11,309
	10 20 30 40 50 60 MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF 70 80 90 100 ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." Bernstein A.M., Whiteheart S.W. Blood 93:571-579(1999) [PubMed: 9885218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Urinary bladder.
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Tissue: Platelet.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells." Ganley I.G., Espinosa E., Pfeffer S.R. J. Cell Biol. 180:159-172(2008) [PubMed: 18195106] [Abstract] Cited for: FUNCTION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U64520 mRNA. Translation: AAB05814.1. BT007327 mRNA. Translation: AAP35991.1. Z98884 Genomic DNA. Translation: CAB63146.1. BC003570 mRNA. Translation: AAH03570.1. BC007050 mRNA. Translation: AAH07050.1.
IPI	IPI00549343.
RefSeq	NP_004772.1. NM_004781.3.
UniGene	Hs.66708.
3D structure databases
ProteinModelPortal	Q15836.
SMR	Q15836. Positions 11-75.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15836. 4 interactions.
STRING	Q15836.
PTM databases
PhosphoSite	Q15836.
Polymorphism databases
DMDM	2501082.
Proteomic databases
PRIDE	Q15836.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000054666; ENSP00000054666; ENSG00000049245.
GeneID	9341.
KEGG	hsa:9341.
UCSC	uc001aol.1. human.
Organism-specific databases
CTD	9341.
GeneCards	GC01P007765.
H-InvDB	HIX0199951.
HGNC	HGNC:12644. VAMP3.
MIM	603657. gene.
neXtProt	NX_Q15836.
PharmGKB	PA37268.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21065.
HOGENOM	HBG386095.
HOVERGEN	HBG006675.
InParanoid	Q15836.
OMA	MWAIGIV.
OrthoDB	EOG43JC6B.
PhylomeDB	Q15836.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6_traffickingpathway. Arf6 trafficking events.
Gene expression databases
ArrayExpress	Q15836.
Bgee	Q15836.
CleanEx	HS_VAMP3.
Genevestigator	Q15836.
GermOnline	ENSG00000049245. Homo sapiens.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
KO	K13505.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	34987.
PMAP-CutDB	Q15836.
SOURCE	Search...

Entry information

Entry name

VAMP3_HUMAN

Accession

Primary (citable) accession number: Q15836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.