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Protein

Vesicle-associated membrane protein 3

Gene

VAMP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.1 Publication

GO - Molecular functioni

GO - Biological processi

  • calcium ion-dependent exocytosis Source: Ensembl
  • exocytosis Source: ProtInc
  • Golgi to plasma membrane protein transport Source: Ensembl
  • membrane fusion Source: ProtInc
  • negative regulation of secretion by cell Source: UniProtKB
  • positive regulation of receptor recycling Source: UniProtKB
  • protein complex assembly Source: ProtInc
  • retrograde transport, endosome to Golgi Source: UniProtKB
  • SNARE complex assembly Source: Ensembl
  • substrate adhesion-dependent cell spreading Source: UniProtKB
  • vesicle docking involved in exocytosis Source: ProtInc
  • vesicle fusion Source: GO_Central
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 3
Short name:
VAMP-3
Alternative name(s):
Cellubrevin
Short name:
CEB
Synaptobrevin-3
Gene namesi
Name:VAMP3
Synonyms:SYB3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:12644. VAMP3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 7776CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei78 – 9821Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini99 – 1002VesicularSequence Analysis

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • clathrin-coated vesicle Source: UniProtKB
  • clathrin-coated vesicle membrane Source: Ensembl
  • cytosol Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • intracellular Source: UniProtKB
  • intracellular membrane-bounded organelle Source: UniProtKB
  • neuron projection Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: MGI
  • secretory granule Source: Ensembl
  • SNARE complex Source: UniProtKB
  • synaptic vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37268.

Polymorphism and mutation databases

BioMutaiVAMP3.
DMDMi2501082.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 10099Vesicle-associated membrane protein 3PRO_0000206728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15836.
PaxDbiQ15836.
PRIDEiQ15836.

PTM databases

PhosphoSiteiQ15836.

Miscellaneous databases

PMAP-CutDBQ15836.

Expressioni

Gene expression databases

BgeeiQ15836.
CleanExiHS_VAMP3.
ExpressionAtlasiQ15836. baseline and differential.
GenevisibleiQ15836. HS.

Interactioni

Subunit structurei

Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with BCAP31; involved in VAMP3 export from the endoplasmic reticulum.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2L13Q9BXK53EBI-722343,EBI-747430
PicalmO550122EBI-722343,EBI-915601From a different organism.
STX4Q128466EBI-722343,EBI-744942

Protein-protein interaction databases

BioGridi114747. 32 interactions.
IntActiQ15836. 14 interactions.
STRINGi9606.ENSP00000054666.

Structurei

3D structure databases

ProteinModelPortaliQ15836.
SMRiQ15836. Positions 11-75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 7461v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG266115.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ15836.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiQ15836.
TreeFamiTF313666.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA
60 70 80 90 100
DALQAGASQF ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS
Length:100
Mass (Da):11,309
Last modified:January 23, 2007 - v3
Checksum:i347E9163157AFF42
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771K → E in AAH05941 (PubMed:16710414).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64520 mRNA. Translation: AAB05814.1.
BT007327 mRNA. Translation: AAP35991.1.
Z98884 Genomic DNA. Translation: CAB63146.1.
BC003570 mRNA. Translation: AAH03570.1.
BC005941 mRNA. Translation: AAH05941.1.
BC007050 mRNA. Translation: AAH07050.1.
CCDSiCCDS88.1.
RefSeqiNP_004772.1. NM_004781.3.
UniGeneiHs.66708.

Genome annotation databases

EnsembliENST00000054666; ENSP00000054666; ENSG00000049245.
GeneIDi9341.
KEGGihsa:9341.
UCSCiuc001aol.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64520 mRNA. Translation: AAB05814.1.
BT007327 mRNA. Translation: AAP35991.1.
Z98884 Genomic DNA. Translation: CAB63146.1.
BC003570 mRNA. Translation: AAH03570.1.
BC005941 mRNA. Translation: AAH05941.1.
BC007050 mRNA. Translation: AAH07050.1.
CCDSiCCDS88.1.
RefSeqiNP_004772.1. NM_004781.3.
UniGeneiHs.66708.

3D structure databases

ProteinModelPortaliQ15836.
SMRiQ15836. Positions 11-75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114747. 32 interactions.
IntActiQ15836. 14 interactions.
STRINGi9606.ENSP00000054666.

PTM databases

PhosphoSiteiQ15836.

Polymorphism and mutation databases

BioMutaiVAMP3.
DMDMi2501082.

Proteomic databases

MaxQBiQ15836.
PaxDbiQ15836.
PRIDEiQ15836.

Protocols and materials databases

DNASUi9341.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000054666; ENSP00000054666; ENSG00000049245.
GeneIDi9341.
KEGGihsa:9341.
UCSCiuc001aol.3. human.

Organism-specific databases

CTDi9341.
GeneCardsiGC01P007765.
HGNCiHGNC:12644. VAMP3.
MIMi603657. gene.
neXtProtiNX_Q15836.
PharmGKBiPA37268.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG266115.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ15836.
KOiK13505.
OMAiFTHACAF.
OrthoDBiEOG7MSMRJ.
PhylomeDBiQ15836.
TreeFamiTF313666.

Miscellaneous databases

ChiTaRSiVAMP3. human.
GeneWikiiVAMP3.
GenomeRNAii9341.
NextBioi34987.
PMAP-CutDBQ15836.
PROiQ15836.
SOURCEiSearch...

Gene expression databases

BgeeiQ15836.
CleanExiHS_VAMP3.
ExpressionAtlasiQ15836. baseline and differential.
GenevisibleiQ15836. HS.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets."
    Bernstein A.M., Whiteheart S.W.
    Blood 93:571-579(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Urinary bladder.
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14.
    Tissue: Platelet.
  6. "A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells."
    Ganley I.G., Espinosa E., Pfeffer S.R.
    J. Cell Biol. 180:159-172(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiVAMP3_HUMAN
AccessioniPrimary (citable) accession number: Q15836
Secondary accession number(s): Q9BRV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.