Q15834 (CC85B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coiled-coil domain-containing protein 85B

Alternative name(s):
Hepatitis delta antigen-interacting protein A
Short name=Delta-interacting protein A

Gene names

Name:	CCDC85B
Synonyms:	DIPA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a transcriptional repressor. May inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. May function in adipocyte differentiation, negatively regulating mitotic clonal expansion. Ref.5 Ref.6
Subunit structure	Interacts with CEBPB By similarity. May interact with CEBPD By similarity. Interacts with MCRS1. Interacts with TCF7L2; competes with CTNNB1. Interacts with the viral phosphoprotein hepatitis delta antigen (HDAG). This interaction affects viral genomic replication in intact cells. Ref.5 Ref.6
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › centrosome Ref.5 Ref.6.
Tissue specificity	Widely expressed including liver.
Induction	Up-regulated by doxorubicin. Ref.6
Miscellaneous	May be the cellular homolog of HDAG. Overexpression inhibited HDV replication, whereas overexpression of HDAG reversed the inhibition, suggesting that HDAG may assist HDV replication by forming a complex with DIPA.
Sequence similarities	Belongs to the CCDC85 family.

Ontologies

Keywords
Biological process	Differentiation Growth regulation Transcription Transcription regulation
Cellular component	Cytoplasm Cytoskeleton Nucleus
Domain	Coiled coil
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell growth Inferred from direct assay Ref.6. Source: UniProtKB negative regulation of fat cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.5. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from direct assay Ref.5. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay Ref.5Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
C19orf25	Q9UFG5	2	EBI-739674,EBI-741214
CHCHD3	Q9NX63	2	EBI-739674,EBI-743375
KANSL1	Q7Z3B3	2	EBI-739674,EBI-740244
KRT17	Q04695	2	EBI-739674,EBI-297873
KRT6A	P02538	2	EBI-739674,EBI-702198
PKN1	Q16512	2	EBI-739674,EBI-602382

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Coiled-coil domain-containing protein 85B		PRO_0000079908
Regions
	Coiled coil	43 – 90	48	Potential
	Coiled coil	118 – 147	30	Potential
Experimental info
	Mutagenesis	131	1	L → A: Loss of interaction with TCF7L2 and loss of suppression of CTNNB1 activity. Loss of cell growth inhibition. Ref.6
	Sequence conflict	34	1	A → T in AAB05928. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q15834 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: EA92D1B712A54047	FASTA	202	22,091
	10 20 30 40 50 60 MEAEAGGLEE LTDEEMAALG KEELVRRLRR EEAARLAALV QRGRLMQEVN RQLQGHLGEI 70 80 90 100 110 120 RELKQLNRRL QAENRELRDL CCFLDSERQR GRRAARQWQL FGTQASRAVR EDLGGCWQKL 130 140 150 160 170 180 AELEGRQEEL LRENLALKEL CLALGEEWGP RGGPSGAGGS GAGPAPELAL PPCGPRDLGD 190 200 GSSSTGSVGS PDQLPLACSP DD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cellular homolog of hepatitis delta antigen: implications for viral replication and evolution." Brazas R., Ganem D. Science 274:90-94(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Promyelocytic leukemia.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"DIPA, which can localize to the centrosome, associates with p78/MCRS1/MSP58 and acts as a repressor of gene transcription." Du X., Wang Q., Hirohashi Y., Greene M.I. Exp. Mol. Pathol. 81:184-190(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MCRS1, SUBCELLULAR LOCATION.
[6]	"Coiled-coil domain containing 85B suppresses the beta-catenin activity in a p53-dependent manner." Iwai A., Hijikata M., Hishiki T., Isono O., Chiba T., Shimotohno K. Oncogene 27:1520-1526(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TCF7L2, INDUCTION BY DOXORUBICIN, MUTAGENESIS OF LEU-131, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63825 mRNA. Translation: AAB05928.1. AK312109 mRNA. Translation: BAG35045.1. CH471076 Genomic DNA. Translation: EAW74468.1. BC008796 mRNA. Translation: AAH08796.1.
IPI	IPI00019976.
RefSeq	NP_006839.2. NM_006848.2.
UniGene	Hs.66713.
3D structure databases
ProteinModelPortal	Q15834.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15834. 125 interactions.
MINT	MINT-1433910.
STRING	9606.ENSP00000311695.
Polymorphism databases
DMDM	92090801.
Proteomic databases
PaxDb	Q15834.
PRIDE	Q15834.
Protocols and materials databases
DNASU	11007.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312579; ENSP00000311695; ENSG00000175602.
GeneID	11007.
KEGG	hsa:11007.
UCSC	uc001ogf.3. human.
Organism-specific databases
CTD	11007.
GeneCards	GC11P065657.
HGNC	HGNC:24926. CCDC85B.
HPA	HPA054415.
MIM	605360. gene.
neXtProt	NX_Q15834.
PharmGKB	PA144596453.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283239.
HOGENOM	HOG000234335.
HOVERGEN	HBG107255.
InParanoid	Q15834.
KO	K16758.
OMA	LVLEEEC.
OrthoDB	EOG4TB4CW.
PhylomeDB	Q15834.
Gene expression databases
Bgee	Q15834.
CleanEx	HS_CCDC85B.
Genevestigator	Q15834.
GermOnline	ENSG00000175602. Homo sapiens.
Family and domain databases
InterPro	IPR019359. DUF2216_coiled-coil. [Graphical view]
Pfam	PF10226. DUF2216. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11007.
NextBio	41813.
SOURCE	Search...

Entry information

Entry name

CC85B_HUMAN

Accession

Primary (citable) accession number: Q15834
Secondary accession number(s): B2R598, Q96HA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.