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Q15819

- UB2V2_HUMAN

UniProt

Q15819 - UB2V2_HUMAN

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Protein

Ubiquitin-conjugating enzyme E2 variant 2

Gene
UBE2V2, MMS2, UEV2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.4 Publications

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. protein binding Source: IntAct

GO - Biological processi

  1. cell proliferation Source: ProtInc
  2. DNA double-strand break processing Source: HGNC
  3. negative regulation of neuron apoptotic process Source: Ensembl
  4. positive regulation of DNA repair Source: Ensembl
  5. positive regulation of neuron projection development Source: Ensembl
  6. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
  7. positive regulation of synapse assembly Source: Ensembl
  8. protein polyubiquitination Source: ProtInc
  9. protein ubiquitination Source: HGNC
  10. regulation of DNA repair Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ15819.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 variant 2
Alternative name(s):
DDVit 1
Enterocyte differentiation-associated factor 1
Short name:
EDAF-1
Enterocyte differentiation-promoting factor 1
Short name:
EDPF-1
MMS2 homolog
Vitamin D3-inducible protein
Gene namesi
Name:UBE2V2
Synonyms:MMS2, UEV2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:12495. UBE2V2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HGNC
  2. extracellular vesicular exosome Source: UniProt
  3. nucleus Source: HGNC
  4. UBC13-MMS2 complex Source: HGNC
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37143.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 145144Ubiquitin-conjugating enzyme E2 variant 2PRO_0000082602Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15819.
PaxDbiQ15819.
PRIDEiQ15819.

PTM databases

PhosphoSiteiQ15819.

Expressioni

Tissue specificityi

Detected in placenta, colon, liver and skin. Detected at very low levels in most tissues.2 Publications

Inductioni

Up-regulated in cultured fresh blood cells upon treatment with vitamin D3.1 Publication

Gene expression databases

ArrayExpressiQ15819.
BgeeiQ15819.
CleanExiHS_UBE2V2.
GenevestigatoriQ15819.

Interactioni

Subunit structurei

Heterodimer with UBE2N. Binds CHFR.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBC35Q94A973EBI-714329,EBI-994120From a different organism.
UBE2NP610883EBI-714329,EBI-1052908

Protein-protein interaction databases

BioGridi113184. 47 interactions.
DIPiDIP-29830N.
IntActiQ15819. 19 interactions.
MINTiMINT-1368221.
STRINGi9606.ENSP00000326473.

Structurei

Secondary structure

1
145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2414
Beta strandi29 – 379
Beta strandi45 – 517
Turni57 – 604
Beta strandi62 – 687
Turni71 – 755
Beta strandi79 – 846
Turni93 – 953
Helixi100 – 1023
Helixi104 – 1074
Helixi115 – 12612
Helixi129 – 1324

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J74X-ray1.90A1-145[»]
1J7DX-ray1.85A1-145[»]
1ZGUNMR-A7-145[»]
3VONX-ray3.15B/D/F/I/K/M/P/R/T/W/Y/a/d/f/h/k/m/o6-143[»]
4ORHX-ray4.80A/E/I1-145[»]
ProteinModelPortaliQ15819.
SMRiQ15819. Positions 6-145.

Miscellaneous databases

EvolutionaryTraceiQ15819.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG239185.
HOVERGENiHBG054552.
InParanoidiQ15819.
KOiK10704.
OMAiIPILAKW.
OrthoDBiEOG77M8R5.
PhylomeDBiQ15819.
TreeFamiTF316971.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15819-1 [UniParc]FASTAAdd to Basket

« Hide

MAVSTGVKVP RNFRLLEELE EGQKGVGDGT VSWGLEDDED MTLTRWTGMI    50
IGPPRTNYEN RIYSLKVECG PKYPEAPPSV RFVTKINMNG INNSSGMVDA 100
RSIPVLAKWQ NSYSIKVVLQ ELRRLMMSKE NMKLPQPPEG QTYNN 145
Length:145
Mass (Da):16,363
Last modified:January 23, 2007 - v4
Checksum:i98D632A1AEC0AADE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti36 – 361E → G.
Corresponds to variant rs11557776 [ dbSNP | Ensembl ].
VAR_052431
Natural varianti40 – 401D → H.
Corresponds to variant rs14890 [ dbSNP | Ensembl ].
VAR_052432
Natural varianti78 – 781P → Q.
Corresponds to variant rs11557786 [ dbSNP | Ensembl ].
VAR_052433

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98091 mRNA. Translation: CAA66717.1.
AF049140 mRNA. Translation: AAC05381.1.
U62136 mRNA. Translation: AAB04758.2.
BT006744 mRNA. Translation: AAP35390.1.
CR407628 mRNA. Translation: CAG28556.1.
BC007051 mRNA. Translation: AAH07051.1.
BC016332 mRNA. Translation: AAH16332.1.
BC016710 mRNA. Translation: AAH16710.1.
BC028673 mRNA. Translation: AAH28673.1.
BC062418 mRNA. Translation: AAH62418.1.
CCDSiCCDS43738.1.
PIRiJC5525.
RefSeqiNP_003341.1. NM_003350.2.
UniGeneiHs.491695.
Hs.595400.

Genome annotation databases

EnsembliENST00000523111; ENSP00000428209; ENSG00000169139.
GeneIDi7336.
KEGGihsa:7336.
UCSCiuc003xqm.3. human.

Polymorphism databases

DMDMi51701935.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98091 mRNA. Translation: CAA66717.1 .
AF049140 mRNA. Translation: AAC05381.1 .
U62136 mRNA. Translation: AAB04758.2 .
BT006744 mRNA. Translation: AAP35390.1 .
CR407628 mRNA. Translation: CAG28556.1 .
BC007051 mRNA. Translation: AAH07051.1 .
BC016332 mRNA. Translation: AAH16332.1 .
BC016710 mRNA. Translation: AAH16710.1 .
BC028673 mRNA. Translation: AAH28673.1 .
BC062418 mRNA. Translation: AAH62418.1 .
CCDSi CCDS43738.1.
PIRi JC5525.
RefSeqi NP_003341.1. NM_003350.2.
UniGenei Hs.491695.
Hs.595400.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J74 X-ray 1.90 A 1-145 [» ]
1J7D X-ray 1.85 A 1-145 [» ]
1ZGU NMR - A 7-145 [» ]
3VON X-ray 3.15 B/D/F/I/K/M/P/R/T/W/Y/a/d/f/h/k/m/o 6-143 [» ]
4ORH X-ray 4.80 A/E/I 1-145 [» ]
ProteinModelPortali Q15819.
SMRi Q15819. Positions 6-145.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113184. 47 interactions.
DIPi DIP-29830N.
IntActi Q15819. 19 interactions.
MINTi MINT-1368221.
STRINGi 9606.ENSP00000326473.

PTM databases

PhosphoSitei Q15819.

Polymorphism databases

DMDMi 51701935.

Proteomic databases

MaxQBi Q15819.
PaxDbi Q15819.
PRIDEi Q15819.

Protocols and materials databases

DNASUi 7336.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000523111 ; ENSP00000428209 ; ENSG00000169139 .
GeneIDi 7336.
KEGGi hsa:7336.
UCSCi uc003xqm.3. human.

Organism-specific databases

CTDi 7336.
GeneCardsi GC08P048970.
HGNCi HGNC:12495. UBE2V2.
MIMi 603001. gene.
neXtProti NX_Q15819.
PharmGKBi PA37143.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG239185.
HOVERGENi HBG054552.
InParanoidi Q15819.
KOi K10704.
OMAi IPILAKW.
OrthoDBi EOG77M8R5.
PhylomeDBi Q15819.
TreeFami TF316971.

Enzyme and pathway databases

Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinki Q15819.

Miscellaneous databases

ChiTaRSi UBE2V2. human.
EvolutionaryTracei Q15819.
GeneWikii UBE2V2.
GenomeRNAii 7336.
NextBioi 28718.
PROi Q15819.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q15819.
Bgeei Q15819.
CleanExi HS_UBE2V2.
Genevestigatori Q15819.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular Cloning of a 1alpha,25-dihydroxyvitamin D3 inducible transcript (DDVit 1) in human blood monocytes."
    Fritsche J., Rehli M., Krause S.W., Andreesen R., Kreutz M.
    Biochem. Biophys. Res. Commun. 235:407-412(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
    Tissue: Blood.
  2. "The products of the yeast MMS2 and two human homologs (hMMS2 and CROC-1) define a structurally and functionally conserved Ubc-like protein family."
    Xiao W., Lin S.L., Broomfield S., Chow B.L., Wei Y.-F.
    Nucleic Acids Res. 26:3908-3914(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Colon carcinoma.
  3. "Isolation and characterization of a putative human enterocyte differentiation promoting factor (EDPF-1)."
    Faria J., Wild G.E.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow, Urinary bladder and Uterus.
  7. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Tissue: Platelet.
  8. "Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair."
    Hofmann R.M., Pickart C.M.
    Cell 96:645-653(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UBE2N.
  9. "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains."
    Bothos J., Summers M.K., Venere M., Scolnick D.M., Halazonetis T.D.
    Oncogene 22:7101-7107(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UBE2N AND CHFR.
  10. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  13. "Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13."
    Moraes T.F., Edwards R.A., McKenna S., Pastushok L., Xiao W., Glover J.N.M., Ellison M.J.
    Nat. Struct. Biol. 8:669-673(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  14. "Molecular insights into the function of RING Finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation."
    Campbell S.J., Edwards R.A., Leung C.C., Neculai D., Hodge C.D., Dhe-Paganon S., Glover J.N.
    J. Biol. Chem. 287:23900-23910(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.8 ANGSTROMS) OF 1-144 IN COMPLEX WITH RNF8 AND UBE2N.
  15. Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 25-271 IN COMPLEX WITH UBE2N AND OTUB1.

Entry informationi

Entry nameiUB2V2_HUMAN
AccessioniPrimary (citable) accession number: Q15819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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