Reviewed,
UniProtKB/Swiss-Prot Q15800 (ERG25_HUMAN)
Last modified
November 25, 2008.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: C-4 methylsterol oxidase EC=1.14.13.72 Alternative name(s): Methylsterol monooxygenase | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 293 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)(+) + H(2)O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)(+) + 2 H(2)O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O(2) = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)(+) + H(2)O. |
| Cofactor | Iron Probable. |
| Pathway | Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6. |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane proteinProbable. |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding. |
| Sequence similarities | Belongs to the sterol desaturase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | Iron NAD |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | fatty acid metabolic process Ref.1 Traceable author statement. Source: ProtInc oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Ref.1 Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Ref.1Traceable author statement. Source: ProtInc plasma membrane Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | C-4 methylsterol oxidase activity Ref.1 Traceable author statement. Source: ProtInc iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 293 | 293 | C-4 methylsterol oxidase | PRO_0000117033 | |||||
Regions | |||||||||
| Transmembrane | 55 – 75 | 21 | Potential | ||||||
| Transmembrane | 100 – 120 | 21 | Potential | ||||||
| Transmembrane | 199 – 219 | 21 | Potential | ||||||
| Motif | 157 – 161 | 5 | Histidine box-1 | ||||||
| Motif | 170 – 174 | 5 | Histidine box-2 | ||||||
| Motif | 249 – 255 | 7 | Histidine box-3 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue." Li L., Kaplan J. J. Biol. Chem. 271:16927-16933(1996) [PubMed: 8663358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine. |
| [2] | Herrmann K. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U60205 mRNA. Translation: AAC50587.1. U93162 mRNA. Translation: AAB81566.1. BC010653 mRNA. Translation: AAH10653.1. BC107879 mRNA. Translation: AAI07880.1. | |
| RefSeq | NP_001017369.1. NP_006736.1. |
| UniGene | Hs.105269 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q15800. |
Genome annotation databases | |
| Ensembl | ENSG00000052802. Homo sapiens. [Contig view] |
| GeneID | 6307. |
| KEGG | hsa:6307. |
Organism-specific databases | |
| H-InvDB | HIX0004617. |
| HGNC | HGNC:10545. SC4MOL. |
| MIM | 607545. gene. |
| PharmGKB | PA34955. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | Q15800. |
| HOVERGEN | Q15800. |
Enzyme and pathway databases | |
| Reactome | REACT_602. Lipid and lipoprotein metabolism. |
Gene expression databases | |
| ArrayExpress | Q15800. |
| CleanEx | HS_SC4MOL. |
| GermOnline | ENSG00000052802. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR006088. Sterol_desatur. [Graphical view] |
| Pfam | PF01598. Sterol_desat. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00157. NADH. |
| LinkHub | Q15800. |
| NextBio | 24483. |
| SOURCE | Search... |
Entry information
| Entry name | ERG25_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15800 Secondary accession number(s): Q32Q24 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


