Reviewed,
UniProtKB/Swiss-Prot Q15800 (ERG25_HUMAN)
Last modified
January 19, 2010.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: C-4 methylsterol oxidase EC=1.14.13.72 Alternative name(s): Methylsterol monooxygenase | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 293 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | 4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O. |
| Cofactor | Iron Probable. |
| Pathway | Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6. |
| Subcellular location | Endoplasmic reticulum membrane; Multi-pass membrane protein Probable. |
| Domain | The histidine box domains may contain the active site and/or be involved in metal ion binding. |
| Sequence similarities | Belongs to the sterol desaturase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Transmembrane |
| Ligand | Iron NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Ref.1 Inferred from Experiment. Source: Reactome integral to membrane Ref.1Traceable author statement. Source: ProtInc plasma membrane Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | C-4 methylsterol oxidase activity Ref.1 Inferred from Experiment. Source: Reactome iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 293 | 293 | C-4 methylsterol oxidase | PRO_0000117033 | |||||
Regions | |||||||||
| Transmembrane | 55 – 75 | 21 | Potential | ||||||
| Transmembrane | 100 – 120 | 21 | Potential | ||||||
| Transmembrane | 199 – 219 | 21 | Potential | ||||||
| Motif | 157 – 161 | 5 | Histidine box-1 | ||||||
| Motif | 170 – 174 | 5 | Histidine box-2 | ||||||
| Motif | 249 – 255 | 7 | Histidine box-3 | ||||||
Natural variations | |||||||||
| Natural variant | 124 | 1 | N → S: dbSNP rs34499452. | VAR_048898 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue." Li L., Kaplan J. J. Biol. Chem. 271:16927-16933(1996) [PubMed: 8663358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine. |
| [2] | Herrmann K. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye. |
| [6] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U60205 mRNA. Translation: AAC50587.1. U93162 mRNA. Translation: AAB81566.1. AK292418 mRNA. Translation: BAF85107.1. CH471056 Genomic DNA. Translation: EAX04820.1. BC010653 mRNA. Translation: AAH10653.1. BC107879 mRNA. Translation: AAI07880.1. |
| IPI | IPI00019899. |
| RefSeq | NP_001017369.1. NP_006736.1. |
| UniGene | Hs.105269 Hs.593050 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q15800. 1 interaction. |
| STRING | Q15800. |
Proteomic databases | |
| PRIDE | Q15800. |
Genome annotation databases | |
| Ensembl | ENST00000261507; ENSP00000261507; ENSG00000052802; Homo sapiens. [Genome view] |
| GeneID | 6307. |
| KEGG | hsa:6307. |
| UCSC | uc003ire.1. human. |
Organism-specific databases | |
| CTD | 6307. |
| GeneCards | GC04P166468. |
| H-InvDB | HIX0004617. |
| HGNC | HGNC:10545. SC4MOL. |
| MIM | 607545. gene. |
| PharmGKB | PA34955. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG10938. |
| HOGENOM | HBG325679. |
| HOVERGEN | Q15800. |
| InParanoid | Q15800. |
| OMA | PLNPLHL. |
| PhylomeDB | Q15800. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.72. 247. |
| Reactome | REACT_602. Metabolism of lipids and lipoproteins. |
Gene expression databases | |
| ArrayExpress | Q15800. |
| Bgee | Q15800. |
| CleanEx | HS_SC4MOL. |
| Genevestigator | Q15800. |
| GermOnline | ENSG00000052802. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR006694. Fatty_acid_hydroxylase. [Graphical view] |
| Pfam | PF04116. FA_hydroxylase. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00157. NADH. |
| NextBio | 24483. |
| SOURCE | Search... |
Entry information
| Entry name | ERG25_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15800 Secondary accession number(s): A8K8Q3, Q32Q24 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


