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Q15784 (NDF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurogenic differentiation factor 2

Short name=NeuroD2
Alternative name(s):
Class A basic helix-loop-helix protein 1
Short name=bHLHa1
NeuroD-related factor
Short name=NDRF
Gene names
Name:NEUROD2
Synonyms:BHLHA1, NDRF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter By similarity.

Subunit structure

Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein By similarity.

Subcellular location

Nucleus By similarity.

Domain

The C-terminus region is necessary for depolarization-induced and calcium-dependent transcription activation By similarity.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processassociative learning

Inferred from electronic annotation. Source: Ensembl

behavioral fear response

Inferred from electronic annotation. Source: Ensembl

cellular response to calcium ion

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to electrical stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

cerebellar cortex development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of synapse maturation

Inferred from sequence or structural similarity. Source: UniProtKB

nervous system development

Traceable author statement Ref.1. Source: ProtInc

neuron development

Inferred from electronic annotation. Source: Ensembl

positive regulation of calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of synapse maturation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of synaptic plasticity

Inferred from sequence or structural similarity. Source: UniProtKB

protein ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Traceable author statement Ref.1. Source: ProtInc

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionE-box binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein heterodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Traceable author statement Ref.1. Source: ProtInc

transcription corepressor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Neurogenic differentiation factor 2
PRO_0000127387

Regions

Domain121 – 17353bHLH
Motif107 – 1137Nuclear localization signal Potential
Compositional bias37 – 4610Poly-Pro
Compositional bias82 – 9110Poly-Glu
Compositional bias282 – 2854Poly-Gly

Experimental info

Sequence conflict1041R → G in AAH22481. Ref.4
Sequence conflict337 – 3382PT → H in BAA77569. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q15784 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: E33AA5C0BE371B47

FASTA38241,361
        10         20         30         40         50         60 
MLTRLFSEPG LLSDVPKFAS WGDGEDDEPR SDKGDAPPPP PPAPGPGAPG PARAAKPVPL 

        70         80         90        100        110        120 
RGEEGTEATL AEVKEEGELG GEEEEEEEEE EGLDEAEGER PKKRGPKKRK MTKARLERSK 

       130        140        150        160        170        180 
LRRQKANARE RNRMHDLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI WALSEILRSG 

       190        200        210        220        230        240 
KRPDLVSYVQ TLCKGLSQPT TNLVAGCLQL NSRNFLTEQG ADGAGRFHGS GGPFAMHPYP 

       250        260        270        280        290        300 
YPCSRLAGAQ CQAAGGLGGG AAHALRTHGY CAAYETLYAA AGGGGASPDY NSSEYEGPLS 

       310        320        330        340        350        360 
PPLCLNGNFS LKQDSSPDHE KSYHYSMHYS ALPGSRPTGH GLVFGSSAVR GGVHSENLLS 

       370        380 
YDMHLHHDRG PMYEELNAFF HN 

« Hide

References

« Hide 'large scale' references
[1]"NeuroD2 and neuroD3: distinct expression patterns and transcriptional activation potentials within the neuroD gene family."
McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D., Tapscott S.J.
Mol. Cell. Biol. 16:5792-5800(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Tapscott S.J., Tamimi R.T., McCormick B.M.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 336-338.
[3]"Identification and analysis of the promoter region of the human NeuroD-related factor (NDRF)."
Kitamura T., Miyachi T., Nakamura S., Kawakami H.
Biochim. Biophys. Acta 1445:142-147(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U58681 Genomic DNA. Translation: AAC26058.1.
AB021742 mRNA. Translation: BAA77569.1.
BC022481 mRNA. Translation: AAH22481.1.
PIRG02668.
RefSeqNP_006151.3. NM_006160.3.
UniGeneHs.322431.

3D structure databases

ProteinModelPortalQ15784.
SMRQ15784. Positions 122-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110834. 2 interactions.
IntActQ15784. 1 interaction.
MINTMINT-6774369.
STRING9606.ENSP00000306754.

PTM databases

PhosphoSiteQ15784.

Polymorphism databases

DMDM6226655.

Proteomic databases

PaxDbQ15784.
PRIDEQ15784.

Protocols and materials databases

DNASU4761.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302584; ENSP00000306754; ENSG00000171532.
GeneID4761.
KEGGhsa:4761.
UCSCuc002hry.3. human.

Organism-specific databases

CTD4761.
GeneCardsGC17M037760.
HGNCHGNC:7763. NEUROD2.
HPAHPA060457.
MIM601725. gene.
neXtProtNX_Q15784.
PharmGKBPA31565.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG268401.
HOGENOMHOG000049256.
HOVERGENHBG000250.
InParanoidQ15784.
KOK09078.
OMACINGNFS.
OrthoDBEOG722J8S.
PhylomeDBQ15784.
TreeFamTF315153.

Gene expression databases

BgeeQ15784.
CleanExHS_NEUROD2.
GenevestigatorQ15784.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR022575. Neurogenic_DUF.
IPR016637. TF_bHLH_NeuroD.
[Graphical view]
PfamPF00010. HLH. 1 hit.
PF12533. Neuro_bHLH. 1 hit.
[Graphical view]
PIRSFPIRSF015618. bHLH_NeuroD. 1 hit.
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNEUROD2.
GenomeRNAi4761.
NextBio18340.
PROQ15784.
SOURCESearch...

Entry information

Entry nameNDF2_HUMAN
AccessionPrimary (citable) accession number: Q15784
Secondary accession number(s): Q8TBI7, Q9UQC6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM