Q15772 (SPEG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Striated muscle preferentially expressed protein kinase
EC=2.7.11.1

Alternative name(s):
Aortic preferentially expressed protein 1
Short name=APEG-1

Gene names

Name:	SPEG
Synonyms:	APEG1, KIAA1297

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	3267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Isoform 3 is found as a monomer or homodimer. Ref.10
Subcellular location	Isoform 3: Nucleus Ref.1.
Tissue specificity	Isoform 1 is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 is predominantly expressed in the aorta. Isoform 3 appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC in vivo. In response to vascular injuries ASMC dedifferentiate and change from a quiescent and contractile phenotype to a proliferative and synthetic phenotype. This proliferation of vascular smooth muscle cells is one of the most prominent features of atherosclerosis. Ref.1 Ref.8
Induction	Isoform 3 is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype. Ref.1
Post-translational modification	May be autophosphorylated.
Miscellaneous	Expression is under the tight control of the locus control region (LCRs).
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 2 fibronectin type-III domains. Contains 9 Ig-like (immunoglobulin-like) domains. Contains 2 protein kinase domains.
Sequence caution	The sequence AAY15052.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABD61734.1 differs from that shown. Reason: Frameshift at positions 31 and 35.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Nucleus
Coding sequence diversity	Alternative promoter usage Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle cell differentiation Inferred from electronic annotation. Source: Compara muscle organ development Traceable author statement Ref.1. Source: ProtInc negative regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	nucleus Traceable author statement Ref.1. Source: ProtInc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 4 (identifier: Q15772-5)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q15772-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-792: Missing.
     961-962: AH → GE
     963-3267: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q15772-4)

Also known as: APEG1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-849: Missing.
     961-962: AH → GE
     963-3267: Missing.

Note: Produced by alternative promoter usage.

Isoform 1 (identifier: Q15772-1)

Also known as: SPEG;

The sequence of this isoform differs from the canonical sequence as follows:
     3209-3267: LQDCLAHPWLQDAYLMKLRRQTLTFTTNRLKEFLGEQRRRRAEAATRHKVLLRSYPGGP → SCLSVCHKEIKMASS

Note: Produced by alternative splicing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 3267

3267

Striated muscle preferentially expressed protein kinase

PRO_0000072666

Regions

Domain

43 – 124

82

Ig-like 1

Domain

722 – 810

89

Ig-like 2

Domain

869 – 958

90

Ig-like 3

Domain

963 – 1057

95

Ig-like 4

Domain

1064 – 1152

89

Ig-like 5

Domain

1188 – 1278

91

Ig-like 6

Domain

1282 – 1377

96

Fibronectin type-III 1

Domain

1384 – 1480

97

Ig-like 7

Domain

1485 – 1573

89

Ig-like 8

Domain

1601 – 1854

254

Protein kinase 1

Domain

2583 – 2673

91

Ig-like 9

Domain

2678 – 2769

92

Fibronectin type-III 2

Domain

2966 – 3218

253

Protein kinase 2

Nucleotide binding

1607 – 1615

9

ATP By similarity

Nucleotide binding

2972 – 2980

9

ATP By similarity

Compositional bias

284 – 345

62

Pro-rich

Compositional bias

525 – 634

110

Pro-rich

Compositional bias

1919 – 1924

6

Poly-Ser

Compositional bias

1925 – 1931

7

Poly-Glu

Compositional bias

2175 – 2316

142

Pro-rich

Compositional bias

2339 – 2503

165

Arg-rich

Compositional bias

2786 – 2965

180

Pro-rich

Sites

Active site

1719

1

Proton acceptor By similarity

Active site

3085

1

Proton acceptor By similarity

Binding site

1630

1

ATP By similarity

Binding site

2995

1

ATP By similarity

Amino acid modifications

Modified residue

313

1

Phosphoserine By similarity

Modified residue

435

1

Phosphoserine By similarity

Modified residue

476

1

Phosphoserine By similarity

Modified residue

1172

1

Phosphoserine By similarity

Disulfide bond

989 ↔ 1041

By similarity

Disulfide bond

2605 ↔ 2657

By similarity

Natural variations

Alternative sequence

1 – 849

849

Missing in isoform 3.

VSP_018258

Alternative sequence

1 – 792

792

Missing in isoform 2.

VSP_018259

Alternative sequence

961 – 962

2

AH → GE in isoform 2 and isoform 3.

VSP_018261

Alternative sequence

963 – 3267

2305

Missing in isoform 2 and isoform 3.

VSP_018262

Alternative sequence

3209 – 3267

59

LQDCL…YPGGP → SCLSVCHKEIKMASS in isoform 1.

VSP_036071

Natural variant

206

1

R → H. Ref.11
Corresponds to variant rs55821435 [ dbSNP | Ensembl ].

VAR_041101

Natural variant

934

1

R → C. Ref.11
Corresponds to variant rs34398769 [ dbSNP | Ensembl ].

VAR_041102

Natural variant

966

1

R → Q. Ref.11
Corresponds to variant rs34861443 [ dbSNP | Ensembl ].

VAR_041103

Natural variant

1103

1

P → L. Ref.11
Corresponds to variant rs56334571 [ dbSNP | Ensembl ].

VAR_041104

Natural variant

1135

1

A → V. Ref.11
Corresponds to variant rs55670811 [ dbSNP | Ensembl ].

VAR_041105

Natural variant

1178

1

E → D in a gastric adenocarcinoma sample; somatic mutation. Ref.11

VAR_041106

Natural variant

1234

1

R → W. Ref.11
Corresponds to variant rs55916864 [ dbSNP | Ensembl ].

VAR_041107

Natural variant

1340

1

R → Q. Ref.11
Corresponds to variant rs34994343 [ dbSNP | Ensembl ].

VAR_041108

Natural variant

1621

1

R → C. Ref.11
Corresponds to variant rs55646900 [ dbSNP | Ensembl ].

VAR_041109

Natural variant

1903

1

R → W in an ovarian mucinous carcinoma sample; somatic mutation. Ref.11

VAR_041110

Natural variant

2189

1

P → L.
Corresponds to variant rs10755037 [ dbSNP | Ensembl ].

VAR_059769

Natural variant

2687

1

P → T. Ref.8 Ref.9 Ref.11
Corresponds to variant rs13026308 [ dbSNP | Ensembl ].

VAR_041111

Natural variant

2742

1

V → M in a gastric adenocarcinoma sample; somatic mutation. Ref.11

VAR_041112

Natural variant

3079

1

H → R. Ref.11
Corresponds to variant rs12464085 [ dbSNP | Ensembl ].

VAR_041113

Experimental info

Sequence conflict

71

1

Q → H in ABD61734. Ref.7

Sequence conflict

73

1

S → I in ABD61734. Ref.7

Sequence conflict

2047

1

S → G in AAT80901. Ref.8

Sequence conflict

2047

1

S → G in BAA92535. Ref.9

Sequence conflict

3005

1

R → P in AAT80901. Ref.8

Sequence conflict

3005

1

R → P in BAA92535. Ref.9

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

3267

Helix Strand Turn

Details...

Beta strand

867 – 873

7

Beta strand

878 – 881

4

Beta strand

886 – 896

11

Beta strand

899 – 904

6

Beta strand

915 – 919

5

Helix

921 – 923

3

Beta strand

924 – 931

8

Helix

934 – 936

3

Beta strand

938 – 946

9

Beta strand

949 – 960

12

Sequences

	Sequence		Length	Mass (Da)
	Isoform 4 [UniParc]. Last modified December 16, 2008. Version 4. Checksum: E67BEB5624144233	FASTA	3,267	354,289
	10 20 30 40 50 60 MQKARGTRGE DAGTRAPPSP GVPPKRAKVG AGGGAPVAVA GAPVFLRPLK NAAVCAGSDV 70 80 90 100 110 120 RLRVVVSGTP QPSLRWFRDG QLLPAPAPEP SCLWLRRCGA QDAGVYSCMA QNERGRASCE 130 140 150 160 170 180 AVLTVLEVGD SETAEDDISD VQGTQRLELR DDGAFSTPTG GSDTLVGTSL DTPPTSVTGT 190 200 210 220 230 240 SEEQVSWWGS GQTVLEQEAG SGGGTRRLPG SPRQAQATGA GPRHLGVEPL VRASRANLVG 250 260 270 280 290 300 ASWGSEDSLS VASDLYGSAF SLYRGRALSI HVSVPQSGLR REEPDLQPQL ASEAPRRPAQ 310 320 330 340 350 360 PPPSKSALLP PPSPRVGKRS PPGPPAQPAA TPTSPHRRTQ EPVLPEDTTT EEKRGKKSKS 370 380 390 400 410 420 SGPSLAGTAE SRPQTPLSEA SGRLSALGRS PRLVRAGSRI LDKLQFFEER RRSLERSDSP 430 440 450 460 470 480 PAPLRPWVPL RKARSLEQPK SERGAPWGTP GASQEELRAP GSVAERRRLF QQKAASLDER 490 500 510 520 530 540 TRQRSPASDL ELRFAQELGR IRRSTSREEL VRSHESLRAT LQRAPSPREP GEPPLFSRPS 550 560 570 580 590 600 TPKTSRAVSP AAAQPPSPSS AEKPGDEPGR PRSRGPAGRT EPGEGPQQEV RRRDQFPLTR 610 620 630 640 650 660 SRAIQECRSP VPPPAADPPE ARTKAPPGRK REPPAQAVRF LPWATPGLEG AAVPQTLEKN 670 680 690 700 710 720 RAGPEAEKRL RRGPEEDGPW GPWDRRGARS QGKGRRARPT SPELESSDDS YVSAGEEPLE 730 740 750 760 770 780 APVFEIPLQN VVVAPGADVL LKCIITANPP PQVSWHKDGS ALRSEGRLLL RAEGERHTLL 790 800 810 820 830 840 LREARAADAG SYMATATNEL GQATCAASLT VRPGGSTSPF SSPITSDEEY LSPPEEFPEP 850 860 870 880 890 900 GETWPRTPTM KPSPSQNRRS SDTGSKAPPT FKVSLMDQSV REGQDVIMSI RVQGEPKPVV 910 920 930 940 950 960 SWLRNRQPVR PDQRRFAEEA EGGLCRLRIL AAERGDAGFY TCKAVNEYGA RQCEARLEVR 970 980 990 1000 1010 1020 AHPESRSLAV LAPLQDVDVG AGEMALFECL VAGPTDVEVD WLCRGRLLQP ALLKCKMHFD 1030 1040 1050 1060 1070 1080 GRKCKLLLTS VHEDDSGVYT CKLSTAKDEL TCSARLTVRP SLAPLFTRLL EDVEVLEGRA 1090 1100 1110 1120 1130 1140 ARFDCKISGT PPPVVTWTHF GCPMEESENL RLRQDGGLHS LHIAHVGSED EGLYAVSAVN 1150 1160 1170 1180 1190 1200 THGQAHCSAQ LYVEEPRTAA SGPSSKLEKM PSIPEEPEQG ELERLSIPDF LRPLQDLEVG 1210 1220 1230 1240 1250 1260 LAKEAMLECQ VTGLPYPTIS WFHNGHRIQS SDDRRMTQYR DVHRLVFPAV GPQHAGVYKS 1270 1280 1290 1300 1310 1320 VIANKLGKAA CYAHLYVTDV VPGPPDGAPQ VVAVTGRMVT LTWNPPRSLD MAIDPDSLTY 1330 1340 1350 1360 1370 1380 TVQHQVLGSD QWTALVTGLR EPGWAATGLR KGVQHIFRVL STTVKSSSKP SPPSEPVQLL 1390 1400 1410 1420 1430 1440 EHGPTLEEAP AMLDKPDIVY VVEGQPASVT VTFNHVEAQV VWRSCRGALL EARAGVYELS 1450 1460 1470 1480 1490 1500 QPDDDQYCLR ICRVSRRDMG ALTCTARNRH GTQTCSVTLE LAEAPRFESI MEDVEVGAGE 1510 1520 1530 1540 1550 1560 TARFAVVVEG KPLPDIMWYK DEVLLTESSH VSFVYEENEC SLVVLSTGAQ DGGVYTCTAQ 1570 1580 1590 1600 1610 1620 NLAGEVSCKA ELAVHSAQTA MEVEGVGEDE DHRGRRLSDF YDIHQEIGRG AFSYLRRIVE 1630 1640 1650 1660 1670 1680 RSSGLEFAAK FIPSQAKPKA SARREARLLA RLQHDCVLYF HEAFERRRGL VIVTELCTEE 1690 1700 1710 1720 1730 1740 LLERIARKPT VCESEIRAYM RQVLEGIHYL HQSHVLHLDV KPENLLVWDG AAGEQQVRIC 1750 1760 1770 1780 1790 1800 DFGNAQELTP GEPQYCQYGT PEFVAPEIVN QSPVSGVTDI WPVGVVAFLC LTGISPFVGE 1810 1820 1830 1840 1850 1860 NDRTTLMNIR NYNVAFEETT FLSLSREARG FLIKVLVQDR LRPTAEETLE HPWFKTQAKG 1870 1880 1890 1900 1910 1920 AEVSTDHLKL FLSRRRWQRS QISYKCHLVL RPIPELLRAP PERVWVTMPR RPPPSGGLSS 1930 1940 1950 1960 1970 1980 SSDSEEEELE ELPSVPRPLQ PEFSGSRVSL TDIPTEDEAL GTPETGAATP MDWQEQGRAP 1990 2000 2010 2020 2030 2040 SQDQEAPSPE ALPSPGQEPA AGASPRRGEL RRGSSAESAL PRAGPRELGR GLHKAASVEL 2050 2060 2070 2080 2090 2100 PQRRSPSPGA TRLARGGLGE GEYAQRLQAL RQRLLRGGPE DGKVSGLRGP LLESLGGRAR 2110 2120 2130 2140 2150 2160 DPRMARAASS EAAPHHQPPL ENRGLQKSSS FSQGEAEPRG RHRRAGAPLE IPVARLGARR 2170 2180 2190 2200 2210 2220 LQESPSLSAL SEAQPSSPAR PSAPKPSTPK SAEPSATTPS DAPQPPAPQP AQDKAPEPRP 2230 2240 2250 2260 2270 2280 EPVRASKPAP PPQALQTLAL PLTPYAQIIQ SLQLSGHAQG PSQGPAAPPS EPKPHAAVFA 2290 2300 2310 2320 2330 2340 RVASPPPGAP EKRVPSAGGP PVLAEKARVP TVPPRPGSSL SSSIENLESE AVFEAKFKRS 2350 2360 2370 2380 2390 2400 RESPLSLGLR LLSRSRSEER GPFRGAEEED GIYRPSPAGT PLELVRRPER SRSVQDLRAV 2410 2420 2430 2440 2450 2460 GEPGLVRRLS LSLSQRLRRT PPAQRHPAWE ARGGDGESSE GGSSARGSPV LAMRRRLSFT 2470 2480 2490 2500 2510 2520 LERLSSRLQR SGSSEDSGGA SGRSTPLFGR LRRATSEGES LRRLGLPHNQ LAAQAGATTP 2530 2540 2550 2560 2570 2580 SAESLGSEAS ATSGSSAPGE SRSRLRWGFS RPRKDKGLSP PNLSASVQEE LGHQYVRSES 2590 2600 2610 2620 2630 2640 DFPPVFHIKL KDQVLLEGEA ATLLCLPAAC PAPHISWMKD KKSLRSEPSV IIVSCKDGRQ 2650 2660 2670 2680 2690 2700 LLSIPRAGKR HAGLYECSAT NVLGSITSSC TVAVARVPGK LAPPEVPQTY QDTALVLWKP 2710 2720 2730 2740 2750 2760 GDSRAPCTYT LERRVDGESV WHPVSSGIPD CYYNVTHLPV GVTVRFRVAC ANRAGQGPFS 2770 2780 2790 2800 2810 2820 NSSEKVFVRG TQDSSAVPSA AHQEAPVTSR PARARPPDSP TSLAPPLAPA APTPPSVTVS 2830 2840 2850 2860 2870 2880 PSSPPTPPSQ ALSSLKAVGP PPQTPPRRHR GLQAARPAEP TLPSTHVTPS EPKPFVLDTG 2890 2900 2910 2920 2930 2940 TPIPASTPQG VKPVSSSTPV YVVTSFVSAP PAPEPPAPEP PPEPTKVTVQ SLSPAKEVVS 2950 2960 2970 2980 2990 3000 SPGSSPRSSP RPEGTTLRQG PPQKPYTFLE EKARGRFGVV RACRENATGR TFVAKIVPYA 3010 3020 3030 3040 3050 3060 AEGKRRVLQE YEVLRTLHHE RIMSLHEAYI TPRYLVLIAE SCGNRELLCG LSDRFRYSED 3070 3080 3090 3100 3110 3120 DVATYMVQLL QGLDYLHGHH VLHLDIKPDN LLLAPDNALK IVDFGSAQPY NPQALRPLGH 3130 3140 3150 3160 3170 3180 RTGTLEFMAP EMVKGEPIGS ATDIWGAGVL TYIMLSGRSP FYEPDPQETE ARIVGGRFDA 3190 3200 3210 3220 3230 3240 FQLYPNTSQS ATLFLRKVLS VHPWSRPSLQ DCLAHPWLQD AYLMKLRRQT LTFTTNRLKE 3250 3260 FLGEQRRRRA EAATRHKVLL RSYPGGP
	Isoform 2 [UniParc]. Checksum: 275949922564F798	FASTA	170	18,673
	10 20 30 40 50 60 MATATNELGQ ATCAASLTVR PGGSTSPFSS PITSDEEYLS PPEEFPEPGE TWPRTPTMKP 70 80 90 100 110 120 SPSQNRRSSD TGSKAPPTFK VSLMDQSVRE GQDVIMSIRV QGEPKPVVSW LRNRQPVRPD 130 140 150 160 170 QRRFAEEAEG GLCRLRILAA ERGDAGFYTC KAVNEYGARQ CEARLEVRGE
	Isoform 3 (APEG1) [UniParc]. Checksum: 04F367263A1397C5	FASTA	113	12,692
	10 20 30 40 50 60 MKPSPSQNRR SSDTGSKAPP TFKVSLMDQS VREGQDVIMS IRVQGEPKPV VSWLRNRQPV 70 80 90 100 110 RPDQRRFAEE AEGGLCRLRI LAAERGDAGF YTCKAVNEYG ARQCEARLEV RGE
	Isoform 1 (SPEG) [UniParc]. Checksum: FBF1188F9F1B883A	FASTA	3,223	348,903
	10 20 30 40 50 60 MQKARGTRGE DAGTRAPPSP GVPPKRAKVG AGGGAPVAVA GAPVFLRPLK NAAVCAGSDV 70 80 90 100 110 120 RLRVVVSGTP QPSLRWFRDG QLLPAPAPEP SCLWLRRCGA QDAGVYSCMA QNERGRASCE 130 140 150 160 170 180 AVLTVLEVGD SETAEDDISD VQGTQRLELR DDGAFSTPTG GSDTLVGTSL DTPPTSVTGT 190 200 210 220 230 240 SEEQVSWWGS GQTVLEQEAG SGGGTRRLPG SPRQAQATGA GPRHLGVEPL VRASRANLVG 250 260 270 280 290 300 ASWGSEDSLS VASDLYGSAF SLYRGRALSI HVSVPQSGLR REEPDLQPQL ASEAPRRPAQ 310 320 330 340 350 360 PPPSKSALLP PPSPRVGKRS PPGPPAQPAA TPTSPHRRTQ EPVLPEDTTT EEKRGKKSKS 370 380 390 400 410 420 SGPSLAGTAE SRPQTPLSEA SGRLSALGRS PRLVRAGSRI LDKLQFFEER RRSLERSDSP 430 440 450 460 470 480 PAPLRPWVPL RKARSLEQPK SERGAPWGTP GASQEELRAP GSVAERRRLF QQKAASLDER 490 500 510 520 530 540 TRQRSPASDL ELRFAQELGR IRRSTSREEL VRSHESLRAT LQRAPSPREP GEPPLFSRPS 550 560 570 580 590 600 TPKTSRAVSP AAAQPPSPSS AEKPGDEPGR PRSRGPAGRT EPGEGPQQEV RRRDQFPLTR 610 620 630 640 650 660 SRAIQECRSP VPPPAADPPE ARTKAPPGRK REPPAQAVRF LPWATPGLEG AAVPQTLEKN 670 680 690 700 710 720 RAGPEAEKRL RRGPEEDGPW GPWDRRGARS QGKGRRARPT SPELESSDDS YVSAGEEPLE 730 740 750 760 770 780 APVFEIPLQN VVVAPGADVL LKCIITANPP PQVSWHKDGS ALRSEGRLLL RAEGERHTLL 790 800 810 820 830 840 LREARAADAG SYMATATNEL GQATCAASLT VRPGGSTSPF SSPITSDEEY LSPPEEFPEP 850 860 870 880 890 900 GETWPRTPTM KPSPSQNRRS SDTGSKAPPT FKVSLMDQSV REGQDVIMSI RVQGEPKPVV 910 920 930 940 950 960 SWLRNRQPVR PDQRRFAEEA EGGLCRLRIL AAERGDAGFY TCKAVNEYGA RQCEARLEVR 970 980 990 1000 1010 1020 AHPESRSLAV LAPLQDVDVG AGEMALFECL VAGPTDVEVD WLCRGRLLQP ALLKCKMHFD 1030 1040 1050 1060 1070 1080 GRKCKLLLTS VHEDDSGVYT CKLSTAKDEL TCSARLTVRP SLAPLFTRLL EDVEVLEGRA 1090 1100 1110 1120 1130 1140 ARFDCKISGT PPPVVTWTHF GCPMEESENL RLRQDGGLHS LHIAHVGSED EGLYAVSAVN 1150 1160 1170 1180 1190 1200 THGQAHCSAQ LYVEEPRTAA SGPSSKLEKM PSIPEEPEQG ELERLSIPDF LRPLQDLEVG 1210 1220 1230 1240 1250 1260 LAKEAMLECQ VTGLPYPTIS WFHNGHRIQS SDDRRMTQYR DVHRLVFPAV GPQHAGVYKS 1270 1280 1290 1300 1310 1320 VIANKLGKAA CYAHLYVTDV VPGPPDGAPQ VVAVTGRMVT LTWNPPRSLD MAIDPDSLTY 1330 1340 1350 1360 1370 1380 TVQHQVLGSD QWTALVTGLR EPGWAATGLR KGVQHIFRVL STTVKSSSKP SPPSEPVQLL 1390 1400 1410 1420 1430 1440 EHGPTLEEAP AMLDKPDIVY VVEGQPASVT VTFNHVEAQV VWRSCRGALL EARAGVYELS 1450 1460 1470 1480 1490 1500 QPDDDQYCLR ICRVSRRDMG ALTCTARNRH GTQTCSVTLE LAEAPRFESI MEDVEVGAGE 1510 1520 1530 1540 1550 1560 TARFAVVVEG KPLPDIMWYK DEVLLTESSH VSFVYEENEC SLVVLSTGAQ DGGVYTCTAQ 1570 1580 1590 1600 1610 1620 NLAGEVSCKA ELAVHSAQTA MEVEGVGEDE DHRGRRLSDF YDIHQEIGRG AFSYLRRIVE 1630 1640 1650 1660 1670 1680 RSSGLEFAAK FIPSQAKPKA SARREARLLA RLQHDCVLYF HEAFERRRGL VIVTELCTEE 1690 1700 1710 1720 1730 1740 LLERIARKPT VCESEIRAYM RQVLEGIHYL HQSHVLHLDV KPENLLVWDG AAGEQQVRIC 1750 1760 1770 1780 1790 1800 DFGNAQELTP GEPQYCQYGT PEFVAPEIVN QSPVSGVTDI WPVGVVAFLC LTGISPFVGE 1810 1820 1830 1840 1850 1860 NDRTTLMNIR NYNVAFEETT FLSLSREARG FLIKVLVQDR LRPTAEETLE HPWFKTQAKG 1870 1880 1890 1900 1910 1920 AEVSTDHLKL FLSRRRWQRS QISYKCHLVL RPIPELLRAP PERVWVTMPR RPPPSGGLSS 1930 1940 1950 1960 1970 1980 SSDSEEEELE ELPSVPRPLQ PEFSGSRVSL TDIPTEDEAL GTPETGAATP MDWQEQGRAP 1990 2000 2010 2020 2030 2040 SQDQEAPSPE ALPSPGQEPA AGASPRRGEL RRGSSAESAL PRAGPRELGR GLHKAASVEL 2050 2060 2070 2080 2090 2100 PQRRSPSPGA TRLARGGLGE GEYAQRLQAL RQRLLRGGPE DGKVSGLRGP LLESLGGRAR 2110 2120 2130 2140 2150 2160 DPRMARAASS EAAPHHQPPL ENRGLQKSSS FSQGEAEPRG RHRRAGAPLE IPVARLGARR 2170 2180 2190 2200 2210 2220 LQESPSLSAL SEAQPSSPAR PSAPKPSTPK SAEPSATTPS DAPQPPAPQP AQDKAPEPRP 2230 2240 2250 2260 2270 2280 EPVRASKPAP PPQALQTLAL PLTPYAQIIQ SLQLSGHAQG PSQGPAAPPS EPKPHAAVFA 2290 2300 2310 2320 2330 2340 RVASPPPGAP EKRVPSAGGP PVLAEKARVP TVPPRPGSSL SSSIENLESE AVFEAKFKRS 2350 2360 2370 2380 2390 2400 RESPLSLGLR LLSRSRSEER GPFRGAEEED GIYRPSPAGT PLELVRRPER SRSVQDLRAV 2410 2420 2430 2440 2450 2460 GEPGLVRRLS LSLSQRLRRT PPAQRHPAWE ARGGDGESSE GGSSARGSPV LAMRRRLSFT 2470 2480 2490 2500 2510 2520 LERLSSRLQR SGSSEDSGGA SGRSTPLFGR LRRATSEGES LRRLGLPHNQ LAAQAGATTP 2530 2540 2550 2560 2570 2580 SAESLGSEAS ATSGSSAPGE SRSRLRWGFS RPRKDKGLSP PNLSASVQEE LGHQYVRSES 2590 2600 2610 2620 2630 2640 DFPPVFHIKL KDQVLLEGEA ATLLCLPAAC PAPHISWMKD KKSLRSEPSV IIVSCKDGRQ 2650 2660 2670 2680 2690 2700 LLSIPRAGKR HAGLYECSAT NVLGSITSSC TVAVARVPGK LAPPEVPQTY QDTALVLWKP 2710 2720 2730 2740 2750 2760 GDSRAPCTYT LERRVDGESV WHPVSSGIPD CYYNVTHLPV GVTVRFRVAC ANRAGQGPFS 2770 2780 2790 2800 2810 2820 NSSEKVFVRG TQDSSAVPSA AHQEAPVTSR PARARPPDSP TSLAPPLAPA APTPPSVTVS 2830 2840 2850 2860 2870 2880 PSSPPTPPSQ ALSSLKAVGP PPQTPPRRHR GLQAARPAEP TLPSTHVTPS EPKPFVLDTG 2890 2900 2910 2920 2930 2940 TPIPASTPQG VKPVSSSTPV YVVTSFVSAP PAPEPPAPEP PPEPTKVTVQ SLSPAKEVVS 2950 2960 2970 2980 2990 3000 SPGSSPRSSP RPEGTTLRQG PPQKPYTFLE EKARGRFGVV RACRENATGR TFVAKIVPYA 3010 3020 3030 3040 3050 3060 AEGKRRVLQE YEVLRTLHHE RIMSLHEAYI TPRYLVLIAE SCGNRELLCG LSDRFRYSED 3070 3080 3090 3100 3110 3120 DVATYMVQLL QGLDYLHGHH VLHLDIKPDN LLLAPDNALK IVDFGSAQPY NPQALRPLGH 3130 3140 3150 3160 3170 3180 RTGTLEFMAP EMVKGEPIGS ATDIWGAGVL TYIMLSGRSP FYEPDPQETE ARIVGGRFDA 3190 3200 3210 3220 FQLYPNTSQS ATLFLRKVLS VHPWSRPSSC LSVCHKEIKM ASS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"APEG-1, a novel gene preferentially expressed in aortic smooth muscle cells, is down-regulated by vascular injury." Hsieh C.-M., Yoshizumi M., Endege W.O., Kho C.-J., Jain M.K., Kashiki S., de Los Santos R., Lee W.-S., Perrella M.A., Lee M.-E. J. Biol. Chem. 271:17354-17359(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cerebellum and Uterus.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[7]	"The human desmin locus: gene organization and LCR-mediated transcriptional control." Tam J.L.Y., Triantaphyllopoulos K., Todd H., Raguz S., de Wit T., Morgan J.E., Partridge T.A., Makrinou E., Grosveld F., Antoniou M. Genomics 87:733-746(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-129 (ISOFORM 1), ALTERNATIVE PROMOTER USAGE.
[8]	"Orthologous relationship of obscurin and Unc-89: phylogeny of a novel family of tandem myosin light chain kinases." Sutter S.B., Raeker M.O., Borisov A.B., Russell M.W. Dev. Genes Evol. 214:352-359(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 130-3267 (ISOFORM 1), TISSUE SPECIFICITY, VARIANT THR-2687.
[9]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 982-3267 (ISOFORM 1), VARIANT THR-2687. Tissue: Brain.
[10]	"X-ray structure of engineered human aortic preferentially expressed protein-1 (APEG-1)." Manjasetty B.A., Niesen F.H., Scheich C., Roske Y., Goetz F., Behlke J., Sievert V., Heinemann U., Bussow K. BMC Struct. Biol. 5:21-21(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.96 ANGSTROMS) OF 864-962, SUBUNIT.
[11]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-206; CYS-934; GLN-966; LEU-1103; VAL-1135; ASP-1178; TRP-1234; GLN-1340; CYS-1621; TRP-1903; THR-2687; MET-2742 AND ARG-3079.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U57099 mRNA. Translation: AAC50599.1.
AK126500 mRNA. Translation: BAC86568.1.
AK289531 mRNA. Translation: BAF82220.1.
CR542201 mRNA. Translation: CAG46998.1.
AC053503 Genomic DNA. Translation: AAY15052.1. Sequence problems.
CH471063 Genomic DNA. Translation: EAW70747.1.
BC006346 mRNA. Translation: AAH06346.1.
DQ395348 mRNA. Translation: ABD61734.1. Frameshift.
AY603755 mRNA. Translation: AAT80901.1.
AB037718 mRNA. Translation: BAA92535.1.

IPI

IPI00178729.
IPI00658151.
IPI00869082.
IPI00915314.

RefSeq

NP_001166947.1. NM_001173476.1.
NP_005867.3. NM_005876.4.

UniGene

Hs.21639.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1U2H	X-ray	0.96	A	864-960	[»]

ProteinModelPortal

Q15772.

SMR

Q15772. Positions 866-961.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15772. 1 interaction.

PTM databases

PhosphoSite

Q15772.

Polymorphism databases

DMDM

218512143.

Proteomic databases

PaxDb

Q15772.

PRIDE

Q15772.

Protocols and materials databases

DNASU

10290.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000312358; ENSP00000311684; ENSG00000072195.
ENST00000396686; ENSP00000379917; ENSG00000072195.
ENST00000396688; ENSP00000379919; ENSG00000072195.
ENST00000396689; ENSP00000379920; ENSG00000072195.
ENST00000396695; ENSP00000379923; ENSG00000072195.

GeneID

10290.

KEGG

hsa:10290.

UCSC

uc002vln.1. human.
uc002vlq.3. human.
uc010fwg.3. human.

Organism-specific databases

CTD

10290.

GeneCards

GC02P220299.

H-InvDB

HIX0002864.

HGNC

HGNC:16901. SPEG.

HPA

HPA018904.

neXtProt

NX_Q15772.

PharmGKB

PA142672598.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOVERGEN

HBG083339.

KO

K08809.

OMA

SAQLYVE.

OrthoDB

EOG4J6RPZ.

Gene expression databases

ArrayExpress

Q15772.

Bgee

Q15772.

CleanEx

HS_SPEG.

Genevestigator

Q15772.

GermOnline

ENSG00000072195. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 11 hits.

InterPro

IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR020675. Myosin_light_ch_kinase-rel.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR015726. Ser/Thr_kin_striated_muscle-sp.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

PANTHER

PTHR22964. PTHR22964. 1 hit.
PTHR22964:SF9. PTHR22964:SF9. 1 hit.

Pfam

PF07679. I-set. 9 hits.
PF00069. Pkinase. 2 hits.
[Graphical view]

SMART

SM00060. FN3. 2 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 6 hits.
SM00220. S_TKc. 2 hits.
[Graphical view]

SUPFAM

SSF49265. FN_III-like. 2 hits.
SSF56112. Kinase_like. 2 hits.

PROSITE

PS50853. FN3. 2 hits.
PS50835. IG_LIKE. 8 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00108. PROTEIN_KINASE_ST. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q15772.

GenomeRNAi

10290.

NextBio

38992.

Entry information

Entry name

SPEG_HUMAN

Accession

Primary (citable) accession number: Q15772
Secondary accession number(s): A8K0G6 , A8MRU0, Q27J74, Q695L1, Q6FGA6, Q6ZQW1, Q6ZTL8, Q9P2P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 16, 2008
Last modified:	April 3, 2013
This is version 125 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.