Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q15771

- RAB30_HUMAN

UniProt

Q15771 - RAB30_HUMAN

Protein

Ras-related protein Rab-30

Gene

RAB30

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (31 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 238GTP
    Nucleotide bindingi64 – 685GTP
    Nucleotide bindingi122 – 1254GTP

    GO - Molecular functioni

    1. GTPase activity Source: ProtInc
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. Golgi organization Source: UniProtKB
    2. GTP catabolic process Source: GOC
    3. protein transport Source: InterPro
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-30
    Gene namesi
    Name:RAB30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:9770. RAB30.

    Subcellular locationi

    Membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatustrans-Golgi network 1 Publication. Cytoplasm 1 Publication. Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. cis-Golgi network Source: UniProtKB
    2. Golgi cisterna Source: UniProtKB
    3. Golgi stack Source: ProtInc
    4. membrane Source: UniProtKB-SubCell
    5. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34121.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Ras-related protein Rab-30PRO_0000121230Add
    BLAST
    Propeptidei201 – 2033Removed in mature formSequence AnalysisPRO_0000370826

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity
    Modified residuei200 – 2001Cysteine methyl esterSequence Analysis
    Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiQ15771.
    PaxDbiQ15771.
    PRIDEiQ15771.

    PTM databases

    PhosphoSiteiQ15771.

    Expressioni

    Gene expression databases

    ArrayExpressiQ15771.
    BgeeiQ15771.
    CleanExiHS_RAB30.
    GenevestigatoriQ15771.

    Organism-specific databases

    HPAiHPA054514.

    Interactioni

    Protein-protein interaction databases

    BioGridi118132. 3 interactions.
    DIPiDIP-60519N.
    IntActiQ15771. 1 interaction.

    Structurei

    Secondary structure

    1
    203
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 1711
    Helixi22 – 3110
    Beta strandi43 – 5311
    Beta strandi56 – 6510
    Helixi69 – 713
    Helixi72 – 754
    Helixi76 – 783
    Beta strandi83 – 908
    Helixi94 – 985
    Helixi100 – 11011
    Beta strandi116 – 1227
    Helixi124 – 1296
    Helixi134 – 14411
    Beta strandi148 – 1503
    Turni153 – 1553
    Helixi159 – 17416

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EW1X-ray2.00A2-184[»]
    ProteinModelPortaliQ15771.
    SMRiQ15771. Positions 5-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ15771.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 469Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiQ15771.
    KOiK07917.
    OMAiHALILVY.
    OrthoDBiEOG7NGQCK.
    PhylomeDBiQ15771.
    TreeFamiTF300097.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q15771-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSMEDYDFLF KIVLIGNAGV GKTCLVRRFT QGLFPPGQGA TIGVDFMIKT    50
    VEINGEKVKL QIWDTAGQER FRSITQSYYR SANALILTYD ITCEESFRCL 100
    PEWLREIEQY ASNKVITVLV GNKIDLAERR EVSQQRAEEF SEAQDMYYLE 150
    TSAKESDNVE KLFLDLACRL ISEARQNTLV NNVSSPLPGE GKSISYLTCC 200
    NFN 203
    Length:203
    Mass (Da):23,058
    Last modified:October 31, 2003 - v2
    Checksum:i935E56DBD48DD7C2
    GO
    Isoform 2 (identifier: Q15771-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         120-164: VGNKIDLAER...ESDNVEKLFL → ATRLTWLKGE...LIMWRNSSLT
         165-203: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:164
    Mass (Da):18,994
    Checksum:i17BE813BEC1CD455
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451D → G in AAC50774. (PubMed:8863739)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei120 – 16445VGNKI…EKLFL → ATRLTWLKGERFPSSELKNS QKLRTCIIWRPQPRNLIMWR NSSLT in isoform 2. 1 PublicationVSP_055833Add
    BLAST
    Alternative sequencei165 – 20339Missing in isoform 2. 1 PublicationVSP_055834Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57092 mRNA. Translation: AAC50774.1.
    AF498956 mRNA. Translation: AAM21104.1.
    CR542106 mRNA. Translation: CAG46903.1.
    BX641138 mRNA. Translation: CAE46061.1.
    AP000893 Genomic DNA. No translation available.
    AP001767 Genomic DNA. No translation available.
    BC014213 mRNA. Translation: AAH14213.1.
    CCDSiCCDS8264.1.
    PIRiJC4962.
    RefSeqiNP_001272988.1. NM_001286059.1.
    NP_001272989.1. NM_001286060.1.
    NP_001272990.1. NM_001286061.1.
    NP_055303.2. NM_014488.4.
    UniGeneiHs.40758.
    Hs.594773.

    Genome annotation databases

    EnsembliENST00000260056; ENSP00000260056; ENSG00000137502. [Q15771-1]
    ENST00000527633; ENSP00000435089; ENSG00000137502. [Q15771-1]
    ENST00000533486; ENSP00000435189; ENSG00000137502. [Q15771-1]
    ENST00000534141; ENSP00000434974; ENSG00000137502. [Q15771-2]
    GeneIDi27314.
    KEGGihsa:27314.
    UCSCiuc001ozu.3. human.

    Polymorphism databases

    DMDMi38258937.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57092 mRNA. Translation: AAC50774.1 .
    AF498956 mRNA. Translation: AAM21104.1 .
    CR542106 mRNA. Translation: CAG46903.1 .
    BX641138 mRNA. Translation: CAE46061.1 .
    AP000893 Genomic DNA. No translation available.
    AP001767 Genomic DNA. No translation available.
    BC014213 mRNA. Translation: AAH14213.1 .
    CCDSi CCDS8264.1.
    PIRi JC4962.
    RefSeqi NP_001272988.1. NM_001286059.1.
    NP_001272989.1. NM_001286060.1.
    NP_001272990.1. NM_001286061.1.
    NP_055303.2. NM_014488.4.
    UniGenei Hs.40758.
    Hs.594773.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EW1 X-ray 2.00 A 2-184 [» ]
    ProteinModelPortali Q15771.
    SMRi Q15771. Positions 5-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118132. 3 interactions.
    DIPi DIP-60519N.
    IntActi Q15771. 1 interaction.

    PTM databases

    PhosphoSitei Q15771.

    Polymorphism databases

    DMDMi 38258937.

    Proteomic databases

    MaxQBi Q15771.
    PaxDbi Q15771.
    PRIDEi Q15771.

    Protocols and materials databases

    DNASUi 27314.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260056 ; ENSP00000260056 ; ENSG00000137502 . [Q15771-1 ]
    ENST00000527633 ; ENSP00000435089 ; ENSG00000137502 . [Q15771-1 ]
    ENST00000533486 ; ENSP00000435189 ; ENSG00000137502 . [Q15771-1 ]
    ENST00000534141 ; ENSP00000434974 ; ENSG00000137502 . [Q15771-2 ]
    GeneIDi 27314.
    KEGGi hsa:27314.
    UCSCi uc001ozu.3. human.

    Organism-specific databases

    CTDi 27314.
    GeneCardsi GC11M082692.
    HGNCi HGNC:9770. RAB30.
    HPAi HPA054514.
    MIMi 605693. gene.
    neXtProti NX_Q15771.
    PharmGKBi PA34121.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi Q15771.
    KOi K07917.
    OMAi HALILVY.
    OrthoDBi EOG7NGQCK.
    PhylomeDBi Q15771.
    TreeFami TF300097.

    Miscellaneous databases

    EvolutionaryTracei Q15771.
    GenomeRNAii 27314.
    NextBioi 50315.
    PROi Q15771.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15771.
    Bgeei Q15771.
    CleanExi HS_RAB30.
    Genevestigatori Q15771.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of two novel rab genes from human melanocytes."
      Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
      Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Melanocyte.
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Uterus.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: B-cell.
    7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Rab30 is required for the morphological integrity of the Golgi apparatus."
      Kelly E.E., Giordano F., Horgan C.P., Jollivet F., Raposo G., McCaffrey M.W.
      Biol. Cell 104:84-101(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "Crystal structure of RAB30 in complex with a GTP analogue."
      Structural genomics consortium (SGC)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-184 IN COMPLEX WITH GTP ANALOG.

    Entry informationi

    Entry nameiRAB30_HUMAN
    AccessioniPrimary (citable) accession number: Q15771
    Secondary accession number(s): Q6FGK1, Q6MZH2, Q96CI8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 31, 2003
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3