Q15771 (RAB30_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-30

Gene names

Name:

RAB30

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins. Ref.8
Subcellular location	Membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus › trans-Golgi network. Cytoplasm. Golgi apparatus Ref.8.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cytoplasm Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype Ref.8. Source: UniProtKB protein transport Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi cisterna Inferred from direct assay Ref.8. Source: UniProtKB cis-Golgi network Inferred from direct assay Ref.8. Source: UniProtKB membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Inferred from direct assay Ref.8. Source: UniProtKB
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement PubMed 9792283. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Ras-related protein Rab-30

PRO_0000121230

Propeptide

201 – 203

3

Removed in mature form Potential

PRO_0000370826

Regions

Nucleotide binding

16 – 23

8

GTP

Nucleotide binding

64 – 68

5

GTP

Nucleotide binding

122 – 125

4

GTP

Motif

38 – 46

9

Effector region By similarity

Amino acid modifications

Modified residue

200

1

Cysteine methyl ester Potential

Lipidation

199

1

S-geranylgeranyl cysteine By similarity

Lipidation

200

1

S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict

45

1

D → G in AAC50774. Ref.1

Secondary structure

1

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203

Helix Strand Turn

Details...

Beta strand

7 – 17

11

Helix

22 – 31

10

Beta strand

43 – 53

11

Beta strand

56 – 65

10

Helix

69 – 71

3

Helix

72 – 75

4

Helix

76 – 78

3

Beta strand

83 – 90

8

Helix

94 – 98

5

Helix

100 – 110

11

Beta strand

116 – 122

7

Helix

124 – 129

6

Helix

134 – 144

11

Beta strand

148 – 150

3

Turn

153 – 155

3

Helix

159 – 174

16

Sequences

	Sequence		Length	Mass (Da)
	Q15771 [UniParc]. Last modified October 31, 2003. Version 2. Checksum: 935E56DBD48DD7C2	FASTA	203	23,058
	10 20 30 40 50 60 MSMEDYDFLF KIVLIGNAGV GKTCLVRRFT QGLFPPGQGA TIGVDFMIKT VEINGEKVKL 70 80 90 100 110 120 QIWDTAGQER FRSITQSYYR SANALILTYD ITCEESFRCL PEWLREIEQY ASNKVITVLV 130 140 150 160 170 180 GNKIDLAERR EVSQQRAEEF SEAQDMYYLE TSAKESDNVE KLFLDLACRL ISEARQNTLV 190 200 NNVSSPLPGE GKSISYLTCC NFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of two novel rab genes from human melanocytes." Chen D., Guo J., Miki T., Tachibana M., Gahl W.A. Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Melanocyte.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Rab30 is required for the morphological integrity of the Golgi apparatus." Kelly E.E., Giordano F., Horgan C.P., Jollivet F., Raposo G., McCaffrey M.W. Biol. Cell 104:84-101(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Crystal structure of RAB30 in complex with a GTP analogue." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-184 IN COMPLEX WITH GTP ANALOG.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U57092 mRNA. Translation: AAC50774.1.
AF498956 mRNA. Translation: AAM21104.1.
CR542106 mRNA. Translation: CAG46903.1.
BC014213 mRNA. Translation: AAH14213.1.

IPI

IPI00302030.

PIR

JC4962.

RefSeq

NP_055303.2. NM_014488.3.

UniGene

Hs.40758.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EW1	X-ray	2.00	A	2-184	[»]

ProteinModelPortal

Q15771.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15771. 1 interaction.

PTM databases

PhosphoSite

Q15771.

Polymorphism databases

DMDM

38258937.

Proteomic databases

PaxDb

Q15771.

PRIDE

Q15771.

Protocols and materials databases

DNASU

27314.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000260056; ENSP00000260056; ENSG00000137502.
ENST00000527633; ENSP00000435089; ENSG00000137502.
ENST00000533486; ENSP00000435189; ENSG00000137502.

GeneID

27314.

KEGG

hsa:27314.

UCSC

uc001ozu.3. human.

Organism-specific databases

CTD

27314.

GeneCards

GC11M082692.

HGNC

HGNC:9770. RAB30.

HPA

HPA054514.

MIM

605693. gene.

neXtProt

NX_Q15771.

PharmGKB

PA34121.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

Q15771.

KO

K07917.

OMA

DYKFLFK.

OrthoDB

EOG43XV4G.

PhylomeDB

Q15771.

Gene expression databases

ArrayExpress

Q15771.

Bgee

Q15771.

CleanEx

HS_RAB30.

Genevestigator

Q15771.

GermOnline

ENSG00000137502. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q15771.

GenomeRNAi

27314.

NextBio

50315.

SOURCE

Search...

Entry information

Entry name

RAB30_HUMAN

Accession

Primary (citable) accession number: Q15771
Secondary accession number(s): Q6FGK1, Q96CI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 31, 2003
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.