Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q15771 (RAB30_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-30
Gene names
Name:RAB30
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins. Ref.8

Subcellular location

Membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatustrans-Golgi network. Cytoplasm. Golgi apparatus Ref.8.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Ras-related protein Rab-30
PRO_0000121230
Propeptide201 – 2033Removed in mature form Potential
PRO_0000370826

Regions

Nucleotide binding16 – 238GTP
Nucleotide binding64 – 685GTP
Nucleotide binding122 – 1254GTP
Motif38 – 469Effector region By similarity

Amino acid modifications

Modified residue2001Cysteine methyl ester Potential
Lipidation1991S-geranylgeranyl cysteine By similarity
Lipidation2001S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict451D → G in AAC50774. Ref.1

Secondary structure

............................... 203
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q15771 [UniParc].

Last modified October 31, 2003. Version 2.
Checksum: 935E56DBD48DD7C2

FASTA20323,058
        10         20         30         40         50         60 
MSMEDYDFLF KIVLIGNAGV GKTCLVRRFT QGLFPPGQGA TIGVDFMIKT VEINGEKVKL 

        70         80         90        100        110        120 
QIWDTAGQER FRSITQSYYR SANALILTYD ITCEESFRCL PEWLREIEQY ASNKVITVLV 

       130        140        150        160        170        180 
GNKIDLAERR EVSQQRAEEF SEAQDMYYLE TSAKESDNVE KLFLDLACRL ISEARQNTLV 

       190        200 
NNVSSPLPGE GKSISYLTCC NFN 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of two novel rab genes from human melanocytes."
Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Melanocyte.
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: B-cell.
[5]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Rab30 is required for the morphological integrity of the Golgi apparatus."
Kelly E.E., Giordano F., Horgan C.P., Jollivet F., Raposo G., McCaffrey M.W.
Biol. Cell 104:84-101(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Crystal structure of RAB30 in complex with a GTP analogue."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-184 IN COMPLEX WITH GTP ANALOG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57092 mRNA. Translation: AAC50774.1.
AF498956 mRNA. Translation: AAM21104.1.
CR542106 mRNA. Translation: CAG46903.1.
BC014213 mRNA. Translation: AAH14213.1.
PIRJC4962.
RefSeqNP_001272988.1. NM_001286059.1.
NP_001272989.1. NM_001286060.1.
NP_001272990.1. NM_001286061.1.
NP_055303.2. NM_014488.4.
UniGeneHs.40758.
Hs.594773.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EW1X-ray2.00A2-184[»]
ProteinModelPortalQ15771.
SMRQ15771. Positions 5-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118132. 3 interactions.
DIPDIP-60519N.
IntActQ15771. 1 interaction.

PTM databases

PhosphoSiteQ15771.

Polymorphism databases

DMDM38258937.

Proteomic databases

PaxDbQ15771.
PRIDEQ15771.

Protocols and materials databases

DNASU27314.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260056; ENSP00000260056; ENSG00000137502.
ENST00000527633; ENSP00000435089; ENSG00000137502.
ENST00000533486; ENSP00000435189; ENSG00000137502.
GeneID27314.
KEGGhsa:27314.
UCSCuc001ozu.3. human.

Organism-specific databases

CTD27314.
GeneCardsGC11M082692.
HGNCHGNC:9770. RAB30.
HPAHPA054514.
MIM605693. gene.
neXtProtNX_Q15771.
PharmGKBPA34121.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidQ15771.
KOK07917.
OMAHALILVY.
OrthoDBEOG7NGQCK.
PhylomeDBQ15771.
TreeFamTF300097.

Gene expression databases

ArrayExpressQ15771.
BgeeQ15771.
CleanExHS_RAB30.
GenevestigatorQ15771.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ15771.
GenomeRNAi27314.
NextBio50315.
PROQ15771.
SOURCESearch...

Entry information

Entry nameRAB30_HUMAN
AccessionPrimary (citable) accession number: Q15771
Secondary accession number(s): Q6FGK1, Q96CI8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 31, 2003
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM