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Q15771

- RAB30_HUMAN

UniProt

Q15771 - RAB30_HUMAN

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Protein

Ras-related protein Rab-30

Gene

RAB30

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 238GTP
Nucleotide bindingi64 – 685GTP
Nucleotide bindingi122 – 1254GTP

GO - Molecular functioni

  1. GTPase activity Source: ProtInc
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. Golgi organization Source: UniProtKB
  2. GTP catabolic process Source: GOC
  3. protein transport Source: InterPro
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-30
Gene namesi
Name:RAB30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:9770. RAB30.

Subcellular locationi

Membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatustrans-Golgi network 1 Publication. Cytoplasm 1 Publication. Golgi apparatus 1 Publication

GO - Cellular componenti

  1. cis-Golgi network Source: UniProtKB
  2. Golgi cisterna Source: UniProtKB
  3. Golgi stack Source: ProtInc
  4. membrane Source: UniProtKB-KW
  5. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34121.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Ras-related protein Rab-30PRO_0000121230Add
BLAST
Propeptidei201 – 2033Removed in mature formSequence AnalysisPRO_0000370826

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity
Modified residuei200 – 2001Cysteine methyl esterSequence Analysis
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ15771.
PaxDbiQ15771.
PRIDEiQ15771.

PTM databases

PhosphoSiteiQ15771.

Expressioni

Gene expression databases

BgeeiQ15771.
CleanExiHS_RAB30.
ExpressionAtlasiQ15771. baseline and differential.
GenevestigatoriQ15771.

Organism-specific databases

HPAiHPA054514.

Interactioni

Protein-protein interaction databases

BioGridi118132. 3 interactions.
DIPiDIP-60519N.
IntActiQ15771. 1 interaction.

Structurei

Secondary structure

1
203
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 1711
Helixi22 – 3110
Beta strandi43 – 5311
Beta strandi56 – 6510
Helixi69 – 713
Helixi72 – 754
Helixi76 – 783
Beta strandi83 – 908
Helixi94 – 985
Helixi100 – 11011
Beta strandi116 – 1227
Helixi124 – 1296
Helixi134 – 14411
Beta strandi148 – 1503
Turni153 – 1553
Helixi159 – 17416

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EW1X-ray2.00A2-184[»]
ProteinModelPortaliQ15771.
SMRiQ15771. Positions 5-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15771.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ15771.
KOiK07917.
OMAiHALILVY.
OrthoDBiEOG7NGQCK.
PhylomeDBiQ15771.
TreeFamiTF300097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q15771-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSMEDYDFLF KIVLIGNAGV GKTCLVRRFT QGLFPPGQGA TIGVDFMIKT
60 70 80 90 100
VEINGEKVKL QIWDTAGQER FRSITQSYYR SANALILTYD ITCEESFRCL
110 120 130 140 150
PEWLREIEQY ASNKVITVLV GNKIDLAERR EVSQQRAEEF SEAQDMYYLE
160 170 180 190 200
TSAKESDNVE KLFLDLACRL ISEARQNTLV NNVSSPLPGE GKSISYLTCC

NFN
Length:203
Mass (Da):23,058
Last modified:October 31, 2003 - v2
Checksum:i935E56DBD48DD7C2
GO
Isoform 2 (identifier: Q15771-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-164: VGNKIDLAER...ESDNVEKLFL → ATRLTWLKGE...LIMWRNSSLT
     165-203: Missing.

Note: No experimental confirmation available.

Show »
Length:164
Mass (Da):18,994
Checksum:i17BE813BEC1CD455
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451D → G in AAC50774. (PubMed:8863739)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei120 – 16445VGNKI…EKLFL → ATRLTWLKGERFPSSELKNS QKLRTCIIWRPQPRNLIMWR NSSLT in isoform 2. 1 PublicationVSP_055833Add
BLAST
Alternative sequencei165 – 20339Missing in isoform 2. 1 PublicationVSP_055834Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57092 mRNA. Translation: AAC50774.1.
AF498956 mRNA. Translation: AAM21104.1.
CR542106 mRNA. Translation: CAG46903.1.
BX641138 mRNA. Translation: CAE46061.1.
AP000893 Genomic DNA. No translation available.
AP001767 Genomic DNA. No translation available.
BC014213 mRNA. Translation: AAH14213.1.
CCDSiCCDS8264.1. [Q15771-1]
PIRiJC4962.
RefSeqiNP_001272988.1. NM_001286059.1. [Q15771-1]
NP_001272989.1. NM_001286060.1. [Q15771-1]
NP_001272990.1. NM_001286061.1. [Q15771-1]
NP_055303.2. NM_014488.4. [Q15771-1]
UniGeneiHs.40758.
Hs.594773.

Genome annotation databases

EnsembliENST00000260056; ENSP00000260056; ENSG00000137502. [Q15771-1]
ENST00000527633; ENSP00000435089; ENSG00000137502. [Q15771-1]
ENST00000533486; ENSP00000435189; ENSG00000137502. [Q15771-1]
ENST00000534141; ENSP00000434974; ENSG00000137502. [Q15771-2]
ENST00000612684; ENSP00000478702; ENSG00000137502. [Q15771-1]
GeneIDi27314.
KEGGihsa:27314.
UCSCiuc001ozu.3. human. [Q15771-1]

Polymorphism databases

DMDMi38258937.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U57092 mRNA. Translation: AAC50774.1 .
AF498956 mRNA. Translation: AAM21104.1 .
CR542106 mRNA. Translation: CAG46903.1 .
BX641138 mRNA. Translation: CAE46061.1 .
AP000893 Genomic DNA. No translation available.
AP001767 Genomic DNA. No translation available.
BC014213 mRNA. Translation: AAH14213.1 .
CCDSi CCDS8264.1. [Q15771-1 ]
PIRi JC4962.
RefSeqi NP_001272988.1. NM_001286059.1. [Q15771-1 ]
NP_001272989.1. NM_001286060.1. [Q15771-1 ]
NP_001272990.1. NM_001286061.1. [Q15771-1 ]
NP_055303.2. NM_014488.4. [Q15771-1 ]
UniGenei Hs.40758.
Hs.594773.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2EW1 X-ray 2.00 A 2-184 [» ]
ProteinModelPortali Q15771.
SMRi Q15771. Positions 5-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 118132. 3 interactions.
DIPi DIP-60519N.
IntActi Q15771. 1 interaction.

PTM databases

PhosphoSitei Q15771.

Polymorphism databases

DMDMi 38258937.

Proteomic databases

MaxQBi Q15771.
PaxDbi Q15771.
PRIDEi Q15771.

Protocols and materials databases

DNASUi 27314.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000260056 ; ENSP00000260056 ; ENSG00000137502 . [Q15771-1 ]
ENST00000527633 ; ENSP00000435089 ; ENSG00000137502 . [Q15771-1 ]
ENST00000533486 ; ENSP00000435189 ; ENSG00000137502 . [Q15771-1 ]
ENST00000534141 ; ENSP00000434974 ; ENSG00000137502 . [Q15771-2 ]
ENST00000612684 ; ENSP00000478702 ; ENSG00000137502 . [Q15771-1 ]
GeneIDi 27314.
KEGGi hsa:27314.
UCSCi uc001ozu.3. human. [Q15771-1 ]

Organism-specific databases

CTDi 27314.
GeneCardsi GC11M082692.
HGNCi HGNC:9770. RAB30.
HPAi HPA054514.
MIMi 605693. gene.
neXtProti NX_Q15771.
PharmGKBi PA34121.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118841.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi Q15771.
KOi K07917.
OMAi HALILVY.
OrthoDBi EOG7NGQCK.
PhylomeDBi Q15771.
TreeFami TF300097.

Miscellaneous databases

EvolutionaryTracei Q15771.
GenomeRNAii 27314.
NextBioi 50315.
PROi Q15771.
SOURCEi Search...

Gene expression databases

Bgeei Q15771.
CleanExi HS_RAB30.
ExpressionAtlasi Q15771. baseline and differential.
Genevestigatori Q15771.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of two novel rab genes from human melanocytes."
    Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
    Gene 174:129-134(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Melanocyte.
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: B-cell.
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Rab30 is required for the morphological integrity of the Golgi apparatus."
    Kelly E.E., Giordano F., Horgan C.P., Jollivet F., Raposo G., McCaffrey M.W.
    Biol. Cell 104:84-101(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Crystal structure of RAB30 in complex with a GTP analogue."
    Structural genomics consortium (SGC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-184 IN COMPLEX WITH GTP ANALOG.

Entry informationi

Entry nameiRAB30_HUMAN
AccessioniPrimary (citable) accession number: Q15771
Secondary accession number(s): Q6FGK1, Q6MZH2, Q96CI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 31, 2003
Last modified: October 29, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3