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Reviewed, UniProtKB/Swiss-Prot Q15768 (EFNB3_HUMAN)

Last modified July 7, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ephrin-B3
Alternative name(s):
    EPH-related receptor tyrosine kinase ligand 8
      Short name=LERK-8
    EPH-related receptor transmembrane ligand ELK-L3
Gene names
Name: EFNB3
Synonyms: EPLG8, LERK8
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons By similarity.

Subunit structure

Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein. Ref.6 Ref.7

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Sequence similarities

Belongs to the ephrin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.5
Chain28 – 340313Ephrin-B3
PRO_0000008395

Regions

Topological domain28 – 226199Extracellular Potential
Transmembrane227 – 24721 Potential
Topological domain248 – 34093Cytoplasmic Potential
Motif338 – 3403PDZ-binding Potential

Amino acid modifications

Glycosylation2101N-linked (GlcNAc...) Potential
Disulfide bond62 ↔ 104 By similarity
Disulfide bond92 ↔ 156 By similarity

Natural variations

Natural variant1661R → Q
VAR_002356

Experimental info

Mutagenesis124 – 1252LW → YM: Complete loss of Nipah protein G binding. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q15768-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EDFF2A23C2FDE79F

FASTA34035,835
        10         20         30         40         50         60 
MGPPHSGPGG VRVGALLLLG VLGLVSGLSL EPVYWNSANK RFQAEGGYVL YPQIGDRLDL 

        70         80         90        100        110        120 
LCPRARPPGP HSSPNYEFYK LYLVGGAQGR RCEAPPAPNL LLTCDRPDLD LRFTIKFQEY 

       130        140        150        160        170        180 
SPNLWGHEFR SHHDYYIIAT SDGTREGLES LQGGVCLTRG MKVLLRVGQS PRGGAVPRKP 

       190        200        210        220        230        240 
VSEMPMERDR GAAHSLEPGK ENLPGDPTSN ATSRGAEGPL PPPSMPAVAG AAGGLALLLL 

       250        260        270        280        290        300 
GVAGAGGAMC WRRRRAKPSE SRHPGPGSFG RGGSLGLGGG GGMGPREAEP GELGIALRGG 

       310        320        330        340 
GAADPPFCPH YEKVSGDYGH PVYIVQDGPP QSPPNIYYKV 

« Hide

References

« Hide 'large scale' references
[1]Cerretti D.P.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"cDNA cloning, chromosomal localization, and expression pattern of EPLG8, a new member of the EPLG gene family encoding ligands of EPH-related protein-tyrosine kinase receptors."
Tang X.X., Pleasure D.E., Ikegaki N.
Genomics 41:17-24(1997) [PubMed: 9126477] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Elk-L3, a novel transmembrane ligand for the Eph family of receptor tyrosine kinases, expressed in embryonic floor plate, roof plate and hindbrain segments."
Gale N.W., Flenniken A., Compton D.C., Jenkins N.A., Copeland N.G., Gilbert D.J., Davis S., Wilkinson D.G., Yancopoulos G.D.
Oncogene 13:1343-1352(1996) [PubMed: 8808709] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain cortex.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42.
[6]"EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains."
Brueckner K., Pablo Labrador J., Scheiffele P., Herb A., Seeburg P.H., Klein R.
Neuron 22:511-524(1999) [PubMed: 10197531] [Abstract]
Cited for: INTERACTION WITH GRIP1 AND GRIP2.
Tissue: Fetal brain.
[7]"Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus."
Negrete O.A., Wolf M.C., Aguilar H.C., Enterlein S., Wang W., Muehlberger E., Su S.V., Bertolotti-Ciarlet A., Flick R., Lee B.
PLoS Pathog. 2:78-86(2006) [PubMed: 16477309] [Abstract]
Cited for: INTERACTION WITH NIPAH VIRUS PROTEIN G, MUTAGENESIS OF 124-LEU-TRP-125.
+Additional computationally mapped references.

Cross-references

Sequence databases

U57001 mRNA. Translation: AAB05170.1.
U66406 mRNA. Translation: AAC51203.1.
U62775 mRNA. Translation: AAC50707.1.
BC022499 mRNA. Translation: AAH22499.1.
BC042944 mRNA. Translation: AAH42944.1.
IPIIPI00019501.
RefSeqNP_001397.1.
UniGeneHs.26988

3D structure databases

HSSPHSSP built from PDB template 1IKO based on UniProtKB P52800.
ModBaseSearch...

PTM databases

PhosphoSiteQ15768.

Proteomic databases

PRIDEQ15768.

Genome annotation databases

EnsemblENSG00000108947. Homo sapiens. [Contig view]
GeneID1949.
KEGGhsa:1949.
UCSCuc002gis.1. human.

Organism-specific databases

GeneCardsGC17P007549.
H-InvDBHIX0021522.
HGNCHGNC:3228. EFNB3.
HPAHPA001623.
MIM602297. gene.
PharmGKBPA27663.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ15768.
HOVERGENQ15768.
OMAQ15768. MGAPHSG.

Enzyme and pathway databases

Pathway_Interaction_DBephbfwdpathway. EPHB forward signaling.

Gene expression databases

ArrayExpressQ15768.
BgeeQ15768.
CleanExHS_EFNB3.
GermOnlineENSG00000108947. Homo sapiens.

Family and domain databases

InterProIPR008972. Cupredoxin.
IPR001799. Ephrin.
IPR019765. Ephrin_CS.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
PANTHERPTHR11304. Ephrin. 1 hit.
PfamPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSPR01347. EPHRIN.
ProDomPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS01299. EPHRIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio7899.
PMAP-CutDBQ15768.
SOURCESearch...

Entry information

Entry nameEFNB3_HUMAN
AccessionPrimary (citable) accession number: Q15768
Secondary accession number(s): O00680, Q8TBH7, Q92875
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 7, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents