Reviewed, UniProtKB/Swiss-Prot Q15762 (CD226_HUMAN)

Names and origin

Protein names

Recommended name:
    CD226 antigen
Alternative name(s):
    DNAX accessory molecule 1
      Short name=DNAM-1
    CD_antigen=CD226

Gene names

Name:	CD226
Synonyms:	DNAM1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
Subunit structure	Interacts with PVR and PVRL2.
Subcellular location	Membrane; Single-pass type I membrane proteinPotential.
Tissue specificity	Expressed by peripheral blood T-lymphocytes.
Post-translational modification	Phosphorylated.
Sequence similarities	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Ref.1 Traceable author statement. Source: ProtInc signal transduction Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 336	318	CD226 antigen		PRO_0000014665
Regions
	Topological domain	19 – 254	236	Extracellular Potential
	Transmembrane	255 – 275	21	Potential
	Topological domain	276 – 336	61	Cytoplasmic Potential
	Domain	19 – 126	108	Ig-like C2-type 1
	Domain	135 – 239	105	Ig-like C2-type 2
	Compositional bias	277 – 284	8	Poly-Arg
Amino acid modifications
	Modified residue	290	1	Phosphoserine
	Modified residue	322	1	Phosphotyrosine
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Disulfide bond	37 ↔ 108		Potential
	Disulfide bond	152 ↔ 222		Potential
Natural variations
	Natural variant	307	1	G → S: dbSNP rs763361.		VAR_018632

Sequences

	Sequence		Length	Mass (Da)
	Q15762-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5379182CC47C11C3	FASTA	336	38,584
	10 20 30 40 50 60 MDYPTLLLAL LHVYRALCEE VLWHTSVPFA ENMSLECVYP SMGILTQVEW FKIGTQQDSI 70 80 90 100 110 120 AIFSPTHGMV IRKPYAERVY FLNSTMASNN MTLFFRNASE DDVGYYSCSL YTYPQGTWQK 130 140 150 160 170 180 VIQVVQSDSF EAAVPSNSHI VSEPGKNVTL TCQPQMTWPV QAVRWEKIQP RQIDLLTYCN 190 200 210 220 230 240 LVHGRNFTSK FPRQIVSNCS HGRWSVIVIP DVTVSDSGLY RCYLQASAGE NETFVMRLTV 250 260 270 280 290 300 AEGKTDNQYT LFVAGGTVLL LLFVISITTI IVIFLNRRRR RERRDLFTES WDTQKAPNNY 310 320 330 RSPISTGQPT NQSMDDTRED IYVNYPTFSR RPKTRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes." Shibuya A., Campbell D., Hannum C., Yssel H., Franz-Bacon K., McClanahan T., Kitamura T., Nicholl J., Sutherland G.R., Lanier L.L., Phillips J.H. Immunity 4:573-581(1996) [PubMed: 8673704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-39; 54-72; 204-221 AND 285-294, FUNCTION, PHOSPHORYLATION, GLYCOSYLATION, TISSUE SPECIFICITY.
[2]	"Receptor involved in the NK cell triggering." Biassoni R. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Natural killer cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112)." Tahara-Hanaoka S., Shibuya K., Onoda Y., Zhang H., Yamazaki S., Miyamoto A., Honda S., Lanier L.L., Shibuya A. Int. Immunol. 16:533-538(2004) [PubMed: 15039383] [Abstract] Cited for: INTERACTION WITH PVR AND PVRL2.
[5]	"PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis." Pende D., Bottino C., Castriconi R., Cantoni C., Marcenaro S., Rivera P., Spaggiari G.M., Dondero A., Carnemolla B., Reymond N., Mingari M.C., Lopez M., Moretta L., Moretta A. Mol. Immunol. 42:463-469(2005) [PubMed: 15607800] [Abstract] Cited for: INTERACTION WITH PVR AND PVRL2.
[6]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290 AND TYR-322, MASS SPECTROMETRY. Tissue: Platelet.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U56102 mRNA. Translation: AAC50560.1. AJ537553 mRNA. Translation: CAD61184.1. BC074787 mRNA. Translation: AAH74787.1.
RefSeq	NP_006557.2.
UniGene	Hs.660130
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q15762.
Genome annotation databases
Ensembl	ENSG00000150637. Homo sapiens. [Contig view]
GeneID	10666.
KEGG	hsa:10666.
Organism-specific databases
H-InvDB	HIX0039717.
HGNC	HGNC:16961. CD226.
HPA	HPA015715.
MIM	605397. gene.
PharmGKB	PA134911130.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q15762.
HOVERGEN	Q15762.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune System.
Gene expression databases
ArrayExpress	Q15762.
CleanEx	HS_CD226.
GermOnline	ENSG00000150637. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003598. Ig_sub2. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF07686. V-set. 2 hits. [Graphical view]
SMART	SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40559.
SOURCE	Search...

Entry information

Entry name

CD226_HUMAN

Accession

Primary (citable) accession number: Q15762

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)