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Q15762 (CD226_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD226 antigen
Alternative name(s):
DNAX accessory molecule 1
Short name=DNAM-1
CD_antigen=CD226
Gene names
Name:CD226
Synonyms:DNAM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Ref.1

Subunit structure

Interacts with PVR and PVRL2. Ref.7 Ref.8

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed by peripheral blood T-lymphocytes. Ref.1

Sequence similarities

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Traceable author statement Ref.1. Source: ProtInc

cell recognition

Traceable author statement PubMed 16304049. Source: BHF-UCL

cytokine production

Inferred from electronic annotation. Source: Ensembl

positive regulation of Fc receptor mediated stimulatory signaling pathway

Inferred from direct assay PubMed 16831868. Source: BHF-UCL

positive regulation of immunoglobulin mediated immune response

Inferred from direct assay PubMed 16831868. Source: BHF-UCL

positive regulation of mast cell activation

Inferred from direct assay PubMed 16831868. Source: BHF-UCL

positive regulation of natural killer cell mediated cytotoxicity

Inferred from mutant phenotype PubMed 16304049. Source: BHF-UCL

positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target

Inferred from mutant phenotype PubMed 12913096. Source: BHF-UCL

regulation of immune response

Traceable author statement. Source: Reactome

signal transduction

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentcell surface

Inferred from direct assay PubMed 16831868. Source: BHF-UCL

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

membrane raft

Traceable author statement PubMed 16831868. Source: BHF-UCL

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functioncell adhesion molecule binding

Inferred from physical interaction PubMed 12913096. Source: BHF-UCL

integrin binding

Inferred from physical interaction PubMed 16831868. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 21982860. Source: IntAct

protein kinase binding

Inferred from physical interaction PubMed 16831868. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PVRL2Q926922EBI-4314442,EBI-718419

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Ref.1
Chain19 – 336318CD226 antigen
PRO_0000014665

Regions

Topological domain19 – 254236Extracellular Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 33661Cytoplasmic Potential
Domain19 – 126108Ig-like C2-type 1
Domain135 – 239105Ig-like C2-type 2
Compositional bias277 – 2848Poly-Arg

Amino acid modifications

Modified residue3221Phosphotyrosine Ref.9 Ref.10
Glycosylation321N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Potential
Glycosylation2311N-linked (GlcNAc...) Potential
Disulfide bond37 ↔ 108 Potential
Disulfide bond152 ↔ 222 Potential

Natural variations

Natural variant3071S → G. Ref.1 Ref.2 Ref.6 Ref.10
Corresponds to variant rs763361 [ dbSNP | Ensembl ].
VAR_018632

Sequences

Sequence LengthMass (Da)Tools
Q15762 [UniParc].

Last modified January 11, 2011. Version 2.
Checksum: 5379182CC4695097

FASTA33638,614
        10         20         30         40         50         60 
MDYPTLLLAL LHVYRALCEE VLWHTSVPFA ENMSLECVYP SMGILTQVEW FKIGTQQDSI 

        70         80         90        100        110        120 
AIFSPTHGMV IRKPYAERVY FLNSTMASNN MTLFFRNASE DDVGYYSCSL YTYPQGTWQK 

       130        140        150        160        170        180 
VIQVVQSDSF EAAVPSNSHI VSEPGKNVTL TCQPQMTWPV QAVRWEKIQP RQIDLLTYCN 

       190        200        210        220        230        240 
LVHGRNFTSK FPRQIVSNCS HGRWSVIVIP DVTVSDSGLY RCYLQASAGE NETFVMRLTV 

       250        260        270        280        290        300 
AEGKTDNQYT LFVAGGTVLL LLFVISITTI IVIFLNRRRR RERRDLFTES WDTQKAPNNY 

       310        320        330 
RSPISTSQPT NQSMDDTRED IYVNYPTFSR RPKTRV 

« Hide

References

« Hide 'large scale' references
[1]"DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes."
Shibuya A., Campbell D., Hannum C., Yssel H., Franz-Bacon K., McClanahan T., Kitamura T., Nicholl J., Sutherland G.R., Lanier L.L., Phillips J.H.
Immunity 4:573-581(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-39; 54-72; 204-221 AND 285-294, FUNCTION, PHOSPHORYLATION, GLYCOSYLATION, TISSUE SPECIFICITY, VARIANT GLY-307.
[2]"Receptor involved in the NK cell triggering."
Biassoni R.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-307.
Tissue: Natural killer cell.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-307.
Tissue: Brain.
[7]"Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112)."
Tahara-Hanaoka S., Shibuya K., Onoda Y., Zhang H., Yamazaki S., Miyamoto A., Honda S., Lanier L.L., Shibuya A.
Int. Immunol. 16:533-538(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PVR AND PVRL2.
[8]"PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis."
Pende D., Bottino C., Castriconi R., Cantoni C., Marcenaro S., Rivera P., Spaggiari G.M., Dondero A., Carnemolla B., Reymond N., Mingari M.C., Lopez M., Moretta L., Moretta A.
Mol. Immunol. 42:463-469(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PVR AND PVRL2.
[9]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-322, VARIANT [LARGE SCALE ANALYSIS] GLY-307, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U56102 mRNA. Translation: AAC50560.1.
AJ537553 mRNA. Translation: CAD61184.1.
AK313199 mRNA. Translation: BAG36015.1.
AC011930 Genomic DNA. No translation available.
AC090231 Genomic DNA. No translation available.
AC091137 Genomic DNA. No translation available.
CH471117 Genomic DNA. Translation: EAW66515.1.
BC074787 mRNA. Translation: AAH74787.1.
CCDSCCDS11997.1.
RefSeqNP_006557.2. NM_006566.2.
XP_005266699.1. XM_005266642.1.
XP_006722436.1. XM_006722373.1.
UniGeneHs.660130.

3D structure databases

ProteinModelPortalQ15762.
SMRQ15762. Positions 24-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115908. 5 interactions.
IntActQ15762. 2 interactions.
STRING9606.ENSP00000280200.

PTM databases

PhosphoSiteQ15762.

Polymorphism databases

DMDM317373338.

Proteomic databases

PaxDbQ15762.
PRIDEQ15762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000280200; ENSP00000280200; ENSG00000150637.
ENST00000582621; ENSP00000461947; ENSG00000150637.
GeneID10666.
KEGGhsa:10666.
UCSCuc002lkm.4. human.

Organism-specific databases

CTD10666.
GeneCardsGC18M067528.
H-InvDBHIX0039717.
HGNCHGNC:16961. CD226.
HPAHPA015715.
MIM605397. gene.
neXtProtNX_Q15762.
PharmGKBPA134911130.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG41580.
HOGENOMHOG000059567.
HOVERGENHBG050849.
InParanoidQ15762.
KOK06567.
OMAEDIYVNY.
PhylomeDBQ15762.
TreeFamTF336260.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ15762.
BgeeQ15762.
CleanExHS_CD226.
GenevestigatorQ15762.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCD226.
GenomeRNAi10666.
NextBio40559.
PROQ15762.
SOURCESearch...

Entry information

Entry nameCD226_HUMAN
AccessionPrimary (citable) accession number: Q15762
Secondary accession number(s): B2R818
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries