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Protein

Neutral amino acid transporter B(0)

Gene

SLC1A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids (PubMed:8702519). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).3 Publications
(Microbial infection) Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses.2 Publications

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: Reactome
  • L-glutamine transmembrane transporter activity Source: BHF-UCL
  • L-serine transmembrane transporter activity Source: Ensembl
  • neutral amino acid transmembrane transporter activity Source: ProtInc
  • receptor activity Source: ProtInc
  • symporter activity Source: UniProtKB-KW
  • virus receptor activity Source: UniProtKB-KW

GO - Biological processi

  • amino acid transport Source: Reactome
  • glutamine secretion Source: Ensembl
  • glutamine transport Source: BHF-UCL
  • L-glutamine import across plasma membrane Source: Ensembl
  • neutral amino acid transport Source: ProtInc

Keywordsi

Molecular functionHost cell receptor for virus entry, Receptor
Biological processAmino-acid transport, Host-virus interaction, Symport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.23.3.3. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral amino acid transporter B(0)
Short name:
ATB(0)
Alternative name(s):
Baboon M7 virus receptor
RD114/simian type D retrovirus receptor
Sodium-dependent neutral amino acid transporter type 2
Solute carrier family 1 member 5
Gene namesi
Name:SLC1A5
Synonyms:ASCT2, M7V1, RDR, RDRC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105281.12.
HGNCiHGNC:10943. SLC1A5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52CytoplasmicSequence analysisAdd BLAST52
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 224ExtracellularSequence analysisAdd BLAST71
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei306 – 326HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6510.
OpenTargetsiENSG00000105281.
PharmGKBiPA35830.

Chemistry databases

ChEMBLiCHEMBL3562162.
DrugBankiDB00174. L-Asparagine.
DB00130. L-Glutamine.
GuidetoPHARMACOLOGYi874.

Polymorphism and mutation databases

BioMutaiSLC1A5.
DMDMi21542389.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020821 – 541Neutral amino acid transporter B(0)Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi212N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei493PhosphoserineCombined sources1
Modified residuei494PhosphothreonineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei535PhosphoserineCombined sources1
Modified residuei539PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ15758.
MaxQBiQ15758.
PaxDbiQ15758.
PeptideAtlasiQ15758.
PRIDEiQ15758.

PTM databases

iPTMnetiQ15758.
PhosphoSitePlusiQ15758.
SwissPalmiQ15758.

Expressioni

Tissue specificityi

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Gene expression databases

BgeeiENSG00000105281.
CleanExiHS_SLC1A5.
ExpressionAtlasiQ15758. baseline and differential.
GenevisibleiQ15758. HS.

Organism-specific databases

HPAiHPA035239.
HPA035240.

Interactioni

Subunit structurei

Homotrimer (Probable). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization (PubMed:23492904).1 Publication1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF5Q999424EBI-356576,EBI-348482

Protein-protein interaction databases

BioGridi112401. 64 interactors.
IntActiQ15758. 48 interactors.
MINTiMINT-5001314.
STRINGi9606.ENSP00000444408.

Chemistry databases

BindingDBiQ15758.

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi180 – 191Combined sources12
Helixi196 – 199Combined sources4
Beta strandi202 – 204Combined sources3
Beta strandi225 – 228Combined sources4
Helixi231 – 246Combined sources16
Helixi249 – 288Combined sources40
Helixi304 – 318Combined sources15
Helixi320 – 329Combined sources10
Helixi333 – 339Combined sources7
Helixi341 – 350Combined sources10
Turni353 – 355Combined sources3
Helixi357 – 365Combined sources9
Turni366 – 368Combined sources3
Helixi372 – 384Combined sources13
Helixi388 – 402Combined sources15
Helixi411 – 427Combined sources17
Helixi434 – 437Combined sources4
Helixi439 – 446Combined sources8
Helixi450 – 452Combined sources3
Helixi453 – 483Combined sources31
Helixi488 – 493Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LLMX-ray3.25A158-230[»]
5LLUX-ray3.32A158-230[»]
5LM4X-ray3.10A158-230[»]
5MJUX-ray3.71A158-230[»]
ProteinModelPortaliQ15758.
SMRiQ15758.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiQ15758.
KOiK05616.
OMAiLVRNIFP.
OrthoDBiEOG091G0UCE.
PhylomeDBiQ15758.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiView protein in InterPro
IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
PfamiView protein in Pfam
PF00375. SDF. 1 hit.
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiView protein in PROSITE
PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Note: A number of isoforms are produced by alternative initiation. Isoforms start at multiple alternative CUG and GUG codons.1 Publication
Isoform 1 (identifier: Q15758-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVADPPRDSK GLAAAEPTAN GGLALASIED QGAAAGGYCG SRDQVRRCLR
60 70 80 90 100
ANLLVLLTVV AVVAGVALGL GVSGAGGALA LGPERLSAFV FPGELLLRLL
110 120 130 140 150
RMIILPLVVC SLIGGAASLD PGALGRLGAW ALLFFLVTTL LASALGVGLA
160 170 180 190 200
LALQPGAASA AINASVGAAG SAENAPSKEV LDSFLDLARN IFPSNLVSAA
210 220 230 240 250
FRSYSTTYEE RNITGTRVKV PVGQEVEGMN ILGLVVFAIV FGVALRKLGP
260 270 280 290 300
EGELLIRFFN SFNEATMVLV SWIMWYAPVG IMFLVAGKIV EMEDVGLLFA
310 320 330 340 350
RLGKYILCCL LGHAIHGLLV LPLIYFLFTR KNPYRFLWGI VTPLATAFGT
360 370 380 390 400
SSSSATLPLM MKCVEENNGV AKHISRFILP IGATVNMDGA ALFQCVAAVF
410 420 430 440 450
IAQLSQQSLD FVKIITILVT ATASSVGAAG IPAGGVLTLA IILEAVNLPV
460 470 480 490 500
DHISLILAVD WLVDRSCTVL NVEGDALGAG LLQNYVDRTE SRSTEPELIQ
510 520 530 540
VKSELPLDPL PVPTEEGNPL LKHYRGPAGD ATVASEKESV M
Length:541
Mass (Da):56,598
Last modified:June 20, 2002 - v2
Checksum:iAD61C789CCFFE934
GO
Isoform 2 (identifier: Q15758-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-203: MVADPPRDSK...SNLVSAAFRS → M

Note: No experimental confirmation available.
Show »
Length:339
Mass (Da):36,636
Checksum:iCFF2D2673EDD4FC9
GO
Isoform 3 (identifier: Q15758-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-228: Missing.

Note: No experimental confirmation available.
Show »
Length:313
Mass (Da):33,710
Checksum:iA2CF681DC2A2F24E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18 – 24TANGGLA → PPTGAWQ in AAC50629 (PubMed:8702519).Curated7
Sequence conflicti44Q → L in AAC50629 (PubMed:8702519).Curated1
Sequence conflicti84 – 87ERLS → GALE in AAC50629 (PubMed:8702519).Curated4
Sequence conflicti341V → A in BAH14917 (PubMed:14702039).Curated1
Sequence conflicti453I → V in AAD09812 (PubMed:10051606).Curated1
Sequence conflicti460D → G in AAD09812 (PubMed:10051606).Curated1
Sequence conflicti463V → A in AAD09812 (PubMed:10051606).Curated1
Sequence conflicti508D → G in AAD09812 (PubMed:10051606).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02043917P → A. Corresponds to variant dbSNP:rs3027956Ensembl.1
Natural variantiVAR_013517512V → LCombined sources1 PublicationCorresponds to variant dbSNP:rs3027961Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0468511 – 228Missing in isoform 3. 1 PublicationAdd BLAST228
Alternative sequenceiVSP_0463541 – 203MVADP…AAFRS → M in isoform 2. 1 PublicationAdd BLAST203

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53347 mRNA. Translation: AAC50629.1.
AF102826 mRNA. Translation: AAD09812.1.
AF105423 mRNA. Translation: AAD27806.1.
GQ919058 mRNA. Translation: ACX53626.1.
AK292690 mRNA. Translation: BAF85379.1.
AK299137 mRNA. Translation: BAG61189.1.
AK301661 mRNA. Translation: BAG63136.1.
AK316546 mRNA. Translation: BAH14917.1.
AC008622 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57446.1.
BC000062 mRNA. Translation: AAH00062.1.
AF334818 mRNA. Translation: AAK77026.1.
CCDSiCCDS12692.1. [Q15758-1]
CCDS46125.1. [Q15758-2]
CCDS46126.1. [Q15758-3]
RefSeqiNP_001138616.1. NM_001145144.1. [Q15758-3]
NP_001138617.1. NM_001145145.1. [Q15758-2]
NP_005619.1. NM_005628.2. [Q15758-1]
UniGeneiHs.631582.

Genome annotation databases

EnsembliENST00000412532; ENSP00000397924; ENSG00000105281. [Q15758-3]
ENST00000434726; ENSP00000406532; ENSG00000105281. [Q15758-2]
ENST00000542575; ENSP00000444408; ENSG00000105281. [Q15758-1]
GeneIDi6510.
KEGGihsa:6510.
UCSCiuc002pfr.4. human. [Q15758-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAAAT_HUMAN
AccessioniPrimary (citable) accession number: Q15758
Secondary accession number(s): A8K9H5
, B4DR77, B4DWS4, B7ZB81, D0EYG6, E9PC01, O95720, Q96RL9, Q9BWQ3, Q9UNP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 20, 2002
Last modified: September 27, 2017
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families