Q15758 (AAAT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutral amino acid transporter B(0)
Short name=ATB(0)

Alternative name(s):
Baboon M7 virus receptor
RD114/simian type D retrovirus receptor
Sodium-dependent neutral amino acid transporter type 2
Solute carrier family 1 member 5

Gene names

Name:	SLC1A5
Synonyms:	ASCT2, M7V1, RDR, RDRC

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. Act as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses. Ref.2 Ref.3
Subcellular location	Membrane; Multi-pass membrane protein Probable. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.10
Tissue specificity	Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A5 subfamily. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative initiation Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Host cell receptor for virus entry Receptor
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular nitrogen compound metabolic process Traceable author statement. Source: Reactome
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA integral to plasma membrane Traceable author statement. Source: ProtInc melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Traceable author statement. Source: ProtInc
Molecular function	neutral amino acid transmembrane transporter activity Traceable author statement. Source: ProtInc receptor activity Traceable author statement. Source: ProtInc sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPDU1	O75352	1	EBI-356576,EBI-1046501
NEK6	Q9HC98	1	EBI-356576,EBI-740364

Alternative products

This entry describes 1 isoform produced by alternative initiation. [Select]

Note: A number of isoforms are produced by alternative initiation. Isoforms start at multiple alternative CUG and GUG codons.

Isoform 1 (identifier: Q15758-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	Neutral amino acid transporter B(0)		PRO_0000202082
Regions
	Topological domain	1 – 52	52	Cytoplasmic Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	99 – 119	21	Helical; Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Topological domain	154 – 224	71	Extracellular Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Transmembrane	377 – 397	21	Helical; Potential
	Transmembrane	399 – 419	21	Helical; Potential
	Transmembrane	426 – 446	21	Helical; Potential
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.12
	Modified residue	503	1	Phosphoserine Ref.14
	Modified residue	532	1	Phosphothreonine Ref.12
	Modified residue	535	1	Phosphoserine Ref.9 Ref.11 Ref.14
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Ref.13
Natural variations
	Natural variant	17	1	P → A. Corresponds to variant rs3027956 [ dbSNP | Ensembl ].		VAR_020439
	Natural variant	512	1	V → L. Corresponds to variant rs3027961 [ dbSNP | Ensembl ].		VAR_013517
Experimental info
	Sequence conflict	18 – 24	7	TANGGLA → PPTGAWQ in AAC50629. Ref.1
	Sequence conflict	44	1	Q → L in AAC50629. Ref.1
	Sequence conflict	84 – 87	4	ERLS → GALE in AAC50629. Ref.1
	Sequence conflict	453	1	I → V in AAD09812. Ref.2
	Sequence conflict	460	1	D → G in AAD09812. Ref.2
	Sequence conflict	463	1	V → A in AAD09812. Ref.2
	Sequence conflict	508	1	D → G in AAD09812. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: AD61C789CCFFE934	FASTA	541	56,598
	10 20 30 40 50 60 MVADPPRDSK GLAAAEPTAN GGLALASIED QGAAAGGYCG SRDQVRRCLR ANLLVLLTVV 70 80 90 100 110 120 AVVAGVALGL GVSGAGGALA LGPERLSAFV FPGELLLRLL RMIILPLVVC SLIGGAASLD 130 140 150 160 170 180 PGALGRLGAW ALLFFLVTTL LASALGVGLA LALQPGAASA AINASVGAAG SAENAPSKEV 190 200 210 220 230 240 LDSFLDLARN IFPSNLVSAA FRSYSTTYEE RNITGTRVKV PVGQEVEGMN ILGLVVFAIV 250 260 270 280 290 300 FGVALRKLGP EGELLIRFFN SFNEATMVLV SWIMWYAPVG IMFLVAGKIV EMEDVGLLFA 310 320 330 340 350 360 RLGKYILCCL LGHAIHGLLV LPLIYFLFTR KNPYRFLWGI VTPLATAFGT SSSSATLPLM 370 380 390 400 410 420 MKCVEENNGV AKHISRFILP IGATVNMDGA ALFQCVAAVF IAQLSQQSLD FVKIITILVT 430 440 450 460 470 480 ATASSVGAAG IPAGGVLTLA IILEAVNLPV DHISLILAVD WLVDRSCTVL NVEGDALGAG 490 500 510 520 530 540 LLQNYVDRTE SRSTEPELIQ VKSELPLDPL PVPTEEGNPL LKHYRGPAGD ATVASEKESV M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line." Kekuda R., Prasad P.D., Fei Y.-J., Torres-Zamorano V., Sinha S., Yang-Feng T.L., Leibach F.H., Ganapathy V. J. Biol. Chem. 271:18657-18661(1996) [PubMed: 8702519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Choriocarcinoma.
[2]	"The RD114/simian type D retrovirus receptor is a neutral amino acid transporter." Rasko J.E.J., Battini J.L., Gottschalk R.J., Mazo I., Miller A.D. Proc. Natl. Acad. Sci. U.S.A. 96:2129-2134(1999) [PubMed: 10051606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION AS A VIRAL RECEPTOR.
[3]	"A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses." Tailor C.S., Nouri A., Zhao Y., Takeuchi Y., Kabat D. J. Virol. 73:4470-4474(1999) [PubMed: 10196349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION AS A VIRAL RECEPTOR.
[4]	"Cloning of ASCT2 cDNA from MCF-7 cells and its expresison in B16 cells." Ouyang D.-Y., Xu L.-H., He X.-H., Zha Q.-B., Guo H., Gao Q., Zhang Y.-T. Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[8]	"Truncated forms of the dual function human ASCT2 neutral amino acid transporter/retroviral receptor are translationally initiated at multiple alternative CUG and GUG codons." Tailor C.S., Marin M., Nouri A., Kavanaugh M.P., Kabat D. J. Biol. Chem. 276:27221-27230(2001) [PubMed: 11350958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-541, ALTERNATIVE INITIATION.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-535, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-532, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[13]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-212, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-503 AND SER-535, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U53347 mRNA. Translation: AAC50629.1. AF102826 mRNA. Translation: AAD09812.1. AF105423 mRNA. Translation: AAD27806.1. GQ919058 mRNA. Translation: ACX53626.1. AK292690 mRNA. Translation: BAF85379.1. CH471126 Genomic DNA. Translation: EAW57446.1. BC000062 mRNA. Translation: AAH00062.1. AF334818 mRNA. Translation: AAK77026.1.
IPI	IPI00019472.
RefSeq	NP_005619.1. NM_005628.2.
UniGene	Hs.631582.
3D structure databases
ProteinModelPortal	Q15758.
SMR	Q15758. Positions 54-486.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15758. 4 interactions.
MINT	MINT-5001314.
STRING	Q15758.
Protein family/group databases
TCDB	2.A.23.3.3. dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family.
PTM databases
PhosphoSite	Q15758.
Polymorphism databases
DMDM	21542389.
Proteomic databases
PeptideAtlas	Q15758.
PRIDE	Q15758.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306894; ENSP00000303623; ENSG00000105281.
GeneID	6510.
KEGG	hsa:6510.
UCSC	uc002pfs.1. human.
Organism-specific databases
CTD	6510.
GeneCards	GC19M047278.
H-InvDB	HIX0015261. HIX0015262.
HGNC	HGNC:10943. SLC1A5.
HPA	HPA035239. HPA035240.
MIM	109190. gene.
neXtProt	NX_Q15758.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11378.
HOVERGEN	HBG000080.
InParanoid	Q15758.
OMA	LLKMIIL.
OrthoDB	EOG4ZKJMB.
PhylomeDB	Q15758.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	Q15758.
Bgee	Q15758.
CleanEx	HS_SLC1A5.
Genevestigator	Q15758.
GermOnline	ENSG00000105281. Homo sapiens.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
KO	K05616.
PANTHER	PTHR11958. Na/diCO_symport. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00174. L-Asparagine. DB00130. L-Glutamine.
NextBio	25315.
SOURCE	Search...

Entry information

Entry name

AAAT_HUMAN

Accession

Primary (citable) accession number: Q15758
Secondary accession number(s): A8K9H5 , D0EYG6, O95720, Q96RL9, Q9BWQ3, Q9UNP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 20, 2002
Last modified:	January 25, 2012
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.