Q15744 (CEBPE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCAAT/enhancer-binding protein epsilon
Short name=C/EBP epsilon

Gene names

Name:

CEBPE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP delta.
Subcellular location	Nucleus.
Tissue specificity	Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Post-translational modification	Phosphorylated.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence AAC51130.1 differs from that shown. Reason: Frameshift at position 4.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cytokine biosynthetic process Inferred from electronic annotation. Source: Compara defense response Traceable author statement Ref.1. Source: ProtInc defense response to bacterium Inferred from electronic annotation. Source: Compara macrophage differentiation Inferred from electronic annotation. Source: Compara phagocytosis Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 281

281

CCAAT/enhancer-binding protein epsilon

PRO_0000076624

Regions

Domain

204 – 267

64

bZIP

Region

208 – 228

21

Basic motif By similarity

Region

230 – 237

8

Leucine-zipper By similarity

Experimental info

Sequence conflict

64

1

P → S in AAC51130. Ref.2

Sequence conflict

68

1

G → R in AAC50708. Ref.1

Sequence conflict

68

1

G → R in AAC50709. Ref.1

Sequence conflict

252

1

Q → E in AAC51130. Ref.2

Secondary structure

1

.

.

.

.

.

281

Helix Strand Turn

Details...

Helix

37 – 47

11

Helix

50 – 57

8

Sequences

	Sequence		Length	Mass (Da)
	Q15744 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: B0A1DFA88C04C3D0	FASTA	281	30,603
	10 20 30 40 50 60 MSHGTYYECE PRGGQQPLEF SGGRAGPGEL GDMCEHEASI DLSAYIESGE EQLLSDLFAV 70 80 90 100 110 120 KPAPEARGLK GPGTPAFPHY LPPDPRPFAY PPHTFGPDRK ALGPGIYSSP GSYDPRAVAV 130 140 150 160 170 180 KEEPRGPEGS RAASRGSYNP LQYQVAHCGQ TAMHLPPTLA APGQPLRVLK APLATAAPPC 190 200 210 220 230 240 SPLLKAPSPA GPLHKGKKAV NKDSLEYRLR RERNNIAVRK SRDKAKRRIL ETQQKVLEYM 250 260 270 280 AENERLRSRV EQLTQELDTL RNLFRQIPEA ANLIKGVGGC S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon, is expressed in cells of lymphoid and myeloid lineages and is localized on chromosome 14q11.2 close to the T-cell receptor alpha/delta locus." Antonson P., Stellan B., Yamanaka R., Xanthopoulos K.G. Genomics 35:30-38(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Cloning of the novel human myeloid-cell-specific C/EBP-epsilon transcription factor." Chumakov A.M., Grillier I., Chumakova E., Chih D., Slater J., Koeffler H.P. Mol. Cell. Biol. 17:1375-1386(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

CEBPEbase

CEBPE mutation db

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U48865 Genomic DNA. Translation: AAC50708.1.
U48866 mRNA. Translation: AAC50709.1.
U80982 Genomic DNA. Translation: AAC51130.1. Frameshift.
BC035797 mRNA. Translation: AAH35797.2.

IPI

IPI00217782.

RefSeq

NP_001796.2. NM_001805.3.

UniGene

Hs.558308.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3T92	X-ray	1.50	A	37-61	[»]

ProteinModelPortal

Q15744.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15744. 7 interactions.

STRING

9606.ENSP00000206513.

PTM databases

PhosphoSite

Q15744.

Polymorphism databases

DMDM

62512175.

Proteomic databases

PRIDE

Q15744.

Protocols and materials databases

DNASU

1053.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000206513; ENSP00000206513; ENSG00000092067.

GeneID

1053.

KEGG

hsa:1053.

UCSC

uc001wiv.2. human.

Organism-specific databases

CTD

1053.

GeneCards

GC14M023586.

HGNC

HGNC:1836. CEBPE.

HPA

CAB005113.
HPA002928.

MIM

600749. gene.

neXtProt

NX_Q15744.

Orphanet

169142. Recurrent infection due to specific granule deficiency.

PharmGKB

PA26379.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG244516.

HOGENOM

HOG000013112.

HOVERGEN

HBG050879.

InParanoid

Q15744.

KO

K10051.

OMA

PAGPLHK.

OrthoDB

EOG4M65JR.

PhylomeDB

Q15744.

Gene expression databases

Bgee

Q15744.

CleanEx

HS_CEBPE.

Genevestigator

Q15744.

GermOnline

ENSG00000092067. Homo sapiens.

Family and domain databases

InterPro

IPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view]

Pfam

PF07716. bZIP_2. 1 hit.
[Graphical view]

PIRSF

PIRSF005879. CCAAT/enhancer-binding. 1 hit.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. False negative.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

1053.

NextBio

4409.

SOURCE

Search...

Entry information

Entry name

CEBPE_HUMAN

Accession

Primary (citable) accession number: Q15744
Secondary accession number(s): Q15745, Q8IYI2, Q99803

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 12, 2005
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.