Reviewed, UniProtKB/Swiss-Prot Q15744 (CEBPE_HUMAN)

Names and origin

Protein names

Recommended name:
    CCAAT/enhancer-binding protein epsilon
      Short name=C/EBP epsilon

Gene names

Name:

CEBPE

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit structure	Binds DNA as a dimer and can form stable heterodimers with C/EBP delta.
Subcellular location	Nucleus.
Tissue specificity	Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Post-translational modification	Phosphorylated.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.
Sequence caution	The sequence AAC51130.1 differs from that shown. Reason: Frameshift at position 4.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: InterPro
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	CCAAT/enhancer-binding protein epsilon		PRO_0000076624
Regions
	Domain	239 – 267	29	Leucine-zipper
	DNA binding	208 – 228	21	Basic motif
Experimental info
	Sequence conflict	64	1	P → S in AAC51130. Ref.2
	Sequence conflict	68	1	G → R in AAC50708 and AAC50709. Ref.1
	Sequence conflict	252	1	Q → E in AAC51130. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q15744-1 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: B0A1DFA88C04C3D0	FASTA	281	30,603
	10 20 30 40 50 60 MSHGTYYECE PRGGQQPLEF SGGRAGPGEL GDMCEHEASI DLSAYIESGE EQLLSDLFAV 70 80 90 100 110 120 KPAPEARGLK GPGTPAFPHY LPPDPRPFAY PPHTFGPDRK ALGPGIYSSP GSYDPRAVAV 130 140 150 160 170 180 KEEPRGPEGS RAASRGSYNP LQYQVAHCGQ TAMHLPPTLA APGQPLRVLK APLATAAPPC 190 200 210 220 230 240 SPLLKAPSPA GPLHKGKKAV NKDSLEYRLR RERNNIAVRK SRDKAKRRIL ETQQKVLEYM 250 260 270 280 AENERLRSRV EQLTQELDTL RNLFRQIPEA ANLIKGVGGC S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human CCAAT/enhancer binding protein gene, C/EBPepsilon, is expressed in cells of lymphoid and myeloid lineages and is localized on chromosome 14q11.2 close to the T-cell receptor alpha/delta locus." Antonson P., Stellan B., Yamanaka R., Xanthopoulos K.G. Genomics 35:30-38(1996) [PubMed: 8661101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Cloning of the novel human myeloid-cell-specific C/EBP-epsilon transcription factor." Chumakov A.M., Grillier I., Chumakova E., Chih D., Slater J., Koeffler H.P. Mol. Cell. Biol. 17:1375-1386(1997) [PubMed: 9032264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

CEBPEbase

CEBPE mutation db

Cross-references

Sequence databases
EMBLGenBankDDBJ	U48865 Genomic DNA. Translation: AAC50708.1. U48866 mRNA. Translation: AAC50709.1. U80982 Genomic DNA. Translation: AAC51130.1. Frameshift. BC035797 mRNA. Translation: AAH35797.2.
RefSeq	NP_001796.2.
UniGene	Hs.558308
3D structure databases
HSSP	HSSP built from PDB template 1H89 based on UniProtKB P17676.
SMR	Q15744. Positions 198-267.
ModBase	Search...
PTM databases
PhosphoSite	Q15744.
Genome annotation databases
Ensembl	ENSG00000092067. Homo sapiens. [Contig view]
GeneID	1053.
KEGG	hsa:1053.
Organism-specific databases
H-InvDB	HIX0037738.
HGNC	HGNC:1836. CEBPE.
HPA	CAB005113. HPA002928.
MIM	600749. gene.
PharmGKB	PA26379.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q15744.
HOVERGEN	Q15744.
Gene expression databases
ArrayExpress	Q15744.
CleanEx	HS_CEBPE.
GermOnline	ENSG00000092067. Homo sapiens.
Family and domain databases
InterPro	IPR011700. bZIP_2. IPR016468. CCAAT/enhancer-binding. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q15744.
NextBio	4409.
SOURCE	Search...

Entry information

Entry name

CEBPE_HUMAN

Accession

Primary (citable) accession number: Q15744
Secondary accession number(s): Q15745, Q8IYI2, Q99803

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	April 12, 2005
Last modified:	November 25, 2008
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)