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Q15743 (OGR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ovarian cancer G-protein coupled receptor 1

Short name=OGR-1
Alternative name(s):
G-protein coupled receptor 68
GPR12A
Sphingosylphosphorylcholine receptor
Gene names
Name:GPR68
Synonyms:OGR1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca2+ mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer By similarity. Ref.8 Ref.10

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Found at low level in a wide range of tissues, but significantly expressed in lung, kidney, bone and nervous system. Ref.10

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Caution

Was originally (Ref.8) thought to be a receptor for sphingosylphosphorylcholine (SPC). However, this work has been retracted (Ref.9).

Sequence caution

The sequence AAH96071.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH96072.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH96073.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH96074.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH98567.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAT38917.1 differs from that shown. Reason: Erroneous initiation.

The sequence ACG60649.1 differs from that shown. Reason: Erroneous initiation.

The sequence EAW81447.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Ovarian cancer G-protein coupled receptor 1
PRO_0000070113

Regions

Topological domain1 – 2121Extracellular Potential
Transmembrane22 – 4625Helical; Name=1; Potential
Topological domain47 – 5812Cytoplasmic Potential
Transmembrane59 – 8022Helical; Name=2; Potential
Topological domain81 – 9515Extracellular Potential
Transmembrane96 – 11722Helical; Name=3; Potential
Topological domain118 – 13619Cytoplasmic Potential
Transmembrane137 – 15822Helical; Name=4; Potential
Topological domain159 – 18325Extracellular Potential
Transmembrane184 – 20522Helical; Name=5; Potential
Topological domain206 – 22823Cytoplasmic Potential
Transmembrane229 – 24921Helical; Name=6; Potential
Topological domain250 – 26314Extracellular Potential
Transmembrane264 – 28421Helical; Name=7; Potential
Topological domain285 – 36581Cytoplasmic Potential

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation81N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 172 By similarity

Natural variations

Natural variant391N → S Found in a renal cell carcinoma case; somatic mutation. Ref.11
VAR_064716
Natural variant531R → Q.
Corresponds to variant rs2230339 [ dbSNP | Ensembl ].
VAR_058714

Experimental info

Mutagenesis171H → F: Failed to stimulate IP formation at pH 6.8, activity is restored at more acid pH. Ref.10
Mutagenesis201H → F: Failed to stimulate IP formation at pH 6.8, activity is restored at more acid pH. Ref.10
Mutagenesis841H → F: Failed to stimulate IP formation at pH 6.8, activity is restored at more acid pH. Ref.10
Mutagenesis891H → F: No effect on pH-sensing activity. Ref.10
Mutagenesis1591H → F: No effect on pH-sensing activity. Ref.10
Mutagenesis1691H → F: Failed to stimulate IP formation at pH 6.8, activity is restored at more acid pH.
Mutagenesis1751H → F: No effect on pH-sensing activity. Ref.10
Mutagenesis2451H → F: Severe loss pH-sensing activity. Ref.10
Mutagenesis2691H → F: Failed to stimulate IP formation at pH 6.8, activity is restored at more acid pH. Ref.10
Sequence conflict140 – 1423GVS → RVT in AAA79060. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q15743 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 05919AFD5B842CCD

FASTA36541,077
        10         20         30         40         50         60 
MGNITADNSS MSCTIDHTIH QTLAPVVYVT VLVVGFPANC LSLYFGYLQI KARNELGVYL 

        70         80         90        100        110        120 
CNLTVADLFY ICSLPFWLQY VLQHDNWSHG DLSCQVCGIL LYENIYISVG FLCCISVDRY 

       130        140        150        160        170        180 
LAVAHPFRFH QFRTLKAAVG VSVVIWAKEL LTSIYFLMHE EVIEDENQHR VCFEHYPIQA 

       190        200        210        220        230        240 
WQRAINYYRF LVGFLFPICL LLASYQGILR AVRRSHGTQK SRKDQIQRLV LSTVVIFLAC 

       250        260        270        280        290        300 
FLPYHVLLLV RSVWEASCDF AKGVFNAYHF SLLLTSFNCV ADPVLYCFVS ETTHRDLARL 

       310        320        330        340        350        360 
RGACLAFLTC SRTGRAREAY PLGAPEASGK SGAQGEEPEL LTKLHPAFQT PNSPGSGGFP 


TGRLA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and tissue distribution of two related G protein-coupled receptor candidates expressed prominently in human lung tissue."
An S., Tsai C., Goetzl E.J.
FEBS Lett. 375:121-124(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of human OGR1, a novel G protein-coupled receptor that maps to chromosome 14."
Xu Y., Casey G.
Genomics 35:397-402(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Ovarian carcinoma.
[3]"Isolation of cDNA coding for human orphan G protein receptor 68."
Kaighin V.A., Martin A.L., Aronstam R.S.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
King M.M., Aronstam R.S., Sharma S.V.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1."
Xu Y., Zhu K., Hong G., Wu W., Baudhuin L.M., Xiao Y.-J., Damron D.S.
Nat. Cell Biol. 2:261-267(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[9]Erratum
Xu Y., Zhu K., Hong G., Wu W., Baudhuin L.M., Xiao Y.-J., Damron D.S.
Nat. Cell Biol. 8:299-299(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: RETRACTION.
[10]"Proton-sensing G-protein-coupled receptors."
Ludwig M.-G., Vanek M., Guerini D., Gasser J.A., Jones C.E., Junker U., Hofstetter H., Wolf R.M., Seuwen K.
Nature 425:93-98(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-17; HIS-20; HIS-84; HIS-89; HIS-159; HIS-175; HIS-245 AND HIS-269.
[11]"Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma."
Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. expand/collapse author list , Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A.
Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT SER-39.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35398 mRNA. Translation: AAA79060.1.
U48405 Genomic DNA. Translation: AAC50596.1.
EU883575 mRNA. Translation: ACG60649.1. Different initiation.
AY615372 mRNA. Translation: AAT38917.1. Different initiation.
AL135818 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81447.1. Different initiation.
BC067472 mRNA. Translation: AAH67472.1.
BC069592 mRNA. Translation: AAH69592.1.
BC096071 mRNA. Translation: AAH96071.1. Different initiation.
BC096072 mRNA. Translation: AAH96072.1. Different initiation.
BC096073 mRNA. Translation: AAH96073.1. Different initiation.
BC096074 mRNA. Translation: AAH96074.1. Different initiation.
BC098567 mRNA. Translation: AAH98567.1. Different initiation.
PIRS68208.
RefSeqNP_001171147.1. NM_001177676.1.
NP_003476.3. NM_003485.3.
XP_005268167.1. XM_005268110.2.
XP_005268168.1. XM_005268111.1.
XP_005268169.1. XM_005268112.1.
UniGeneHs.8882.

3D structure databases

ProteinModelPortalQ15743.
SMRQ15743. Positions 1-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000238699.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM3024266.

Proteomic databases

PaxDbQ15743.
PRIDEQ15743.

Protocols and materials databases

DNASU8111.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000238699; ENSP00000238699; ENSG00000119714.
ENST00000531499; ENSP00000434045; ENSG00000119714.
ENST00000535815; ENSP00000440797; ENSG00000119714.
GeneID8111.
KEGGhsa:8111.
UCSCuc001xzg.3. human.

Organism-specific databases

CTD8111.
GeneCardsGC14M091699.
HGNCHGNC:4519. GPR68.
MIM601404. gene.
neXtProtNX_Q15743.
PharmGKBPA28911.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149070.
HOGENOMHOG000004801.
InParanoidQ15743.
KOK08408.
OrthoDBEOG7992QK.
PhylomeDBQ15743.
TreeFamTF331803.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ15743.
BgeeQ15743.
CleanExHS_GPR68.
GenevestigatorQ15743.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005389. OGR1_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01564. OGR1RECEPTOR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR68.
GenomeRNAi8111.
NextBio30763.
PROQ15743.
SOURCESearch...

Entry information

Entry nameOGR1_HUMAN
AccessionPrimary (citable) accession number: Q15743
Secondary accession number(s): Q13334, Q4VBB4, Q6IX34
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries